Back to Search
Start Over
MicroRNA-936/ERBB4/Akt axis exhibits anticancer properties of gastric cancer through inhibition of cell proliferation, migration, and invasion
- Source :
- Kaohsiung Journal of Medical Sciences, Vol 37, Iss 2, Pp 111-120 (2021)
- Publication Year :
- 2020
-
Abstract
- Gastric cancer is one of the most common cancers globally and has a poor prognosis. MiR‐936 has been reported to regulate cell activity and tumor progression in non‐small cell lung cancer, glioma, and epithelial ovarian cancer. However, the specific role and mechanism of miR‐936 in gastric cancer have not been explored. In present study, gastric cancer cells were transfected with miR‐936 mimic, and cell proliferation, cell cycle distribution, cell apoptosis, migration and invasion were assessed via cell‐counting kit‐8, flow cytometry, wound healing, and transwell assay, respectively. Dual luciferase reporter assay was used to check miR‐936 binding to its downstream target. It was shown that miR‐936 was downregulated in gastric cancer tissues and cells. Erb‐B2 Receptor Tyrosine Kinase 4 (ERBB4) was confirmed as a direct target of miR‐936 and negatively regulated its expression by miR‐936. Overexpression of miR‐936 suppressed cell proliferation, cell cycle progression, cell migration and invasion, and enhanced cell apoptosis in gastric cancer cells, which could be reversed by further ERBB4 overexpression. Western blot results showed that miR‐936/ERBB4 axis regulated Akt‐related pathways to control gastric cancer cell activities. Therefore, our data suggest that miR‐936 overexpression inhibits cell proliferation and invasion and promotes cell apoptosis through Akt‐related pathways by targeting ERBB4, which provides novel insight to target miR‐936 or miR‐936/ERBB4 axis for the treatment of gastric cancer.
- Subjects :
- Receptor, ErbB-4
miR‐936
Down-Regulation
Apoptosis
03 medical and health sciences
0302 clinical medicine
Cell Movement
Stomach Neoplasms
Cell Line, Tumor
medicine
Humans
Neoplasm Invasiveness
Protein kinase B
ERBB4
Cell Proliferation
lcsh:R5-920
Base Sequence
business.industry
Cell growth
gastric cancer
Cancer
Cell migration
General Medicine
Cell cycle
medicine.disease
Up-Regulation
Gene Expression Regulation, Neoplastic
MicroRNAs
Tumor progression
030220 oncology & carcinogenesis
Cancer cell
Cancer research
030211 gastroenterology & hepatology
business
lcsh:Medicine (General)
Proto-Oncogene Proteins c-akt
Signal Transduction
Subjects
Details
- ISSN :
- 24108650
- Volume :
- 37
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- The Kaohsiung journal of medical sciencesREFERENCES
- Accession number :
- edsair.doi.dedup.....1fe23c76ee7e74be0788060d10e0b014