54 results on '"Lucia Anemona"'
Search Results
2. Macrophage Activation and M2 Polarization in Wound Bed of Diabetic Patients Treated by Dermal/Epidermal Substitute Nevelia
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Marco Meloni, Alessandro Mauriello, Francesca Servadei, Manuela Montanaro, Manuel Scimeca, Laura Giurato, Artem Smirnov, Eleonora Candi, Valentina Izzo, Luigi Uccioli, and Lucia Anemona
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Wound Healing ,0303 health sciences ,business.industry ,Macrophages ,Macrophage polarization ,General Medicine ,M2 polarization ,Macrophage Activation ,Pharmacology ,medicine.disease ,Diabetic foot ,Diabetic Foot ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,Diabetes Mellitus ,Humans ,Medicine ,Macrophage ,Surgery ,business ,Wound healing ,030304 developmental biology - Abstract
Clinical evidences have shown good results using dermal/epidermal substitutes (DESs) to treat diabetic foot ulcers. Recent studies suggest that, in addition to their scaffold action, DESs may favor wound healing by influencing wound bed inflammatory cells. This study aims to investigate whether DES may influence the inflammatory infiltrate and macrophages polarization toward a reparative phenotype. Fifteen diabetic patients with chronic foot ulcers have been randomly enrolled: 5 treated only by standard of care, served as control group (CG), and 10 treated with DES composed of type 1 bovin collagen (Nevelia, SYMATESE) considered as test group (TG). A biopsy was taken at baseline (T0) and after 30 days (T1). From bioptic paraffin specimen histological, immunohistochemical, and immunofluorescence analysis was performed. Immunohistochemistry reactions evaluated the number of M1 macrophage (CD38+) and M2 macrophage (CD163+). TG patients displayed general macrophage activation and their greater polarization toward M2 subpopulation 30 days after DES implant, compared with CG. From T0 to T1 there was a significant decrease of CD38+ (230 ± 42 and 135 ± 48 mm2, respectively; P < .001) and significant increase of CD163+ (102 ± 21 positive cells/mm2 and 366 ± 42 positive cells/mm2, respectively; P < .001). Confocal microscopy confirmed an increase of M2 cells as expressed by the reduced CD68+/CD163+ ratio. After 6 months of observation 6 patients (60%) of the TG completely healed, while only 1 patient (20%) healed in the CG ( P < .01). The tested DES makes possible to treat diabetic foot ulcers inducing tissue reparative processes through macrophage activation and M2 reparative polarization.
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- 2020
3. Panniculitis-Like Presentation of Extracavitary Primary Effusion Lymphoma
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Monia Di Prete, Lucia Anemona, Simone Facchetti, Vito Mario Rapisarda, and Andrea Saggini
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Adult ,Male ,Pathology ,medicine.medical_specialty ,Panniculitis ,Skin Neoplasms ,HIV Infections ,Dermatology ,Pathology and Forensic Medicine ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Immunophenotyping ,immune system diseases ,Antiretroviral Therapy, Highly Active ,Lymphoma, Primary Effusion ,hemic and lymphatic diseases ,medicine ,Humans ,integumentary system ,business.industry ,virus diseases ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,medicine.disease ,Lymphoma ,Serous fluid ,Effusion ,Primary effusion lymphoma ,Presentation (obstetrics) ,Differential diagnosis ,business - Abstract
Primary effusion lymphoma (PEL) is defined as a HHV-8-associated large B-cell lymphoma, which favors HIV-infected young adults, typically presenting as a serous (pleural, pericardial, or peritoneal) effusion with no identifiable tumor mass. Uncommon instances of lymphoid proliferations with the same morphology, immunophenotype, and molecular features as PEL, but occurring as a solid tumor mass without serous cavities involvement, have been termed extracavitary (or solid) variant of PEL. We hereby report the exceptional case of a HIV-associated extracavitary PEL primarily localized to the skin and exhibiting a panniculitis-like presentation. Primary cutaneous presentation of extracavitary PEL is exceedingly uncommon, with only 6 cases previously described in the literature. In light of its atypical immunophenotype, the differential diagnosis in case of skin involvement by extracavitary PEL is challenging: demonstration of HHV-8 infection in neoplastic cells is of pivotal importance. Our case is further atypical in that the lymphoid proliferation underwent complete and protracted regression solely by establishment of highly active antiretroviral therapy.
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- 2020
4. A Prospective Evaluation of Tru-Cut Biopsy and Fine-needle Aspiration Cytology in Male Breast Cancer Detection
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Feliciana Lamacchia, Giulia Claroni, Francesca Servadei, Chiara Adriana Pistolese, Alberto Collura, Marco Pellicciaro, Lucia Anemona, Marco Materazzo, Michela Censi, Gianluca Vanni, and Tommaso Perretta
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Male ,Cancer Research ,medicine.medical_specialty ,Cytodiagnosis ,Biopsy, Fine-Needle ,Breast Neoplasms ,Malignancy ,Sensitivity and Specificity ,General Biochemistry, Genetics and Molecular Biology ,Prospective evaluation ,Breast Neoplasms, Male ,03 medical and health sciences ,0302 clinical medicine ,Fine needle aspiration cytology ,Biopsy ,medicine ,Humans ,Prospective Studies ,skin and connective tissue diseases ,Trucut biopsy ,Pharmacology ,medicine.diagnostic_test ,business.industry ,Carcinoma ,Ductal carcinoma ,medicine.disease ,Aspiration cytology ,body regions ,030220 oncology & carcinogenesis ,Male breast cancer ,Radiology ,business ,Research Article - Abstract
Background: Male breast-cancer (MBC) is often diagnosed late. Our purpose was to evaluate fine-needle aspiration cytology (FNAC) versus Tru-Cut biopsy (TCNB) in MBC diagnosis. Patients and Methods: Men with suspicious breast lesions were prospectively enrolled; 54 met the inclusion criteria and underwent FNAC and TCNB. FNAC, TCNB and gold-standard results were compared. Results: Unsatisfactory results were 11.1% after FNAC and none after TCNB (p=0.027). After gold-standard evaluation, the diagnosis of FNAC and TCNB was confirmed, respectively, in 63.0% and 98.1% and changed in 37.0% and 1.9% (p
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- 2020
5. Does Age Matter? Estimating Risks of Locoregional Recurrence After Breast-conservative Surgery
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Rosaria Meucci, Agostino Chiaravalloti, Marco Materazzo, Paolo Orsaria, Gianluca Vanni, Ljuba Morando, Jonathan Caspi, Marco Pellicciaro, Lucia Anemona, Rosaria Barbarino, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Ilaria Portarena, Camilla Deiana, Maria Rolando D'Angelillo, and Tommaso Perretta
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Risk ,Cancer Research ,medicine.medical_specialty ,Breast Neoplasms ,Subgroup analysis ,Mastectomy, Segmental ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Biomarkers, Tumor ,medicine ,Humans ,Operation time ,In patient ,Aged ,Retrospective Studies ,Aged, 80 and over ,Postoperative Care ,Pharmacology ,business.industry ,Incidence (epidemiology) ,Middle Aged ,Prognosis ,Tailored treatment ,medicine.disease ,Surgery ,Breast conservative surgery ,Receptors, Estrogen ,Case-Control Studies ,030220 oncology & carcinogenesis ,Ki67 index ,Female ,Neoplasm Recurrence, Local ,business ,Research Article - Abstract
Background/Aim: In 2016, in the United States, more than 50% of breast cancer (BC) cases were diagnosed in patients older than 60 years of age. Our study aimed to estimate the risk of locoregional recurrence (LR) in patients who underwent breast-conservative treatment (BCT), according to age. Patients and Methods: This retrospective monocentric study analyzed 613 cases of patients who underwent BCT between 2003 and 2014. Patients were divided into groups according to age: Under70 (under 70 years old) and Over70 (above 70 years old). Margins width, histology results, prognostic and predictive factors were compared. Subgroup analysis was performed for patients who experienced LR. Results: LR Incidence among Under70 and Over70 was 5.4% and 1.7%, respectively (p
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- 2020
6. High Sensitivity C-Reactive Protein Increases the Risk of Carotid Plaque Instability in Male Dyslipidemic Patients
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Francesca Servadei, Marina Cardellini, Rita Bonfiglio, Manuela Montanaro, Roberto Bei, Viviana Casagrande, Arnaldo Ippoliti, Alessandro Mauriello, Vittorio Manzari, Erica Giacobbi, Lucia Anemona, Rossella Menghini, Orazio Schillaci, Eugenio Martelli, Nicoletta Urbano, Massimo Federici, Manuel Scimeca, and Elena Bonanno
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cardiovascular risk factors ,Medicine (General) ,medicine.medical_specialty ,Plaque instability ,carotid plaque ,medicine.medical_treatment ,Clinical Biochemistry ,Settore MED/08 ,Carotid endarterectomy ,Disease ,dyslipidemic patients ,Settore MED/04 ,Asymptomatic ,Article ,R5-920 ,Internal medicine ,Diabetes mellitus ,Medicine ,Kidney ,biology ,business.industry ,C-reactive protein ,medicine.disease ,hs-CRP ,Settore MED/46 ,medicine.anatomical_structure ,Cardiology ,biology.protein ,medicine.symptom ,atherosclerosis ,business ,Lipoprotein - Abstract
Background: The aim of this study was to evaluate how the high sensitivity C-reactive protein (hs-CRP) values influence the risk of carotid plaque instability in association with other cardiovascular risk factors. Methods: One hundred and fifty-six carotid plaques from both symptomatic and asymptomatic patients requiring surgical carotid endarterectomy were retrospectively collected. According to the modified American Heart Association, atherosclerosis plaques have been histologically distinguished into unstable and stable. The following anamnestic and hematochemical data were also considered: age, gender, hypertension, diabetes mellitus, smoking habit, therapy, low-density lipoprotein (LDL)-C, kidney failure and hs-CRP. Results: The results of our study clearly show that high levels of hs-CRP significantly increase the carotid plaque instability in dyslipidemic patients. Specifically, a 67% increase of the risk of carotid plaque instability was observed in patients with high LDL-C. Therefore, the highest risk was observed in male dyslipidemic patients 2333 (95% CI 0.73–7.48) and in aged female patients 2713 (95% CI 0.14–53.27). Discussion: These data strongly suggest a biological relationship between the hs-CRP values and the alteration of lipidic metabolism mostly in male patients affected by carotid atherosclerosis. The measurement of hs-CRP might be useful as a potential screening tool in the prevention of atheroscletotic disease.
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- 2021
7. The risk of carotid plaque instability in patients with metabolic syndrome is higher in women with hypertriglyceridemia
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Massimo Federici, Manuel Scimeca, Alessandro Mauriello, Manuela Montanaro, Rita Bonfiglio, Annalisa Noce, Arnaldo Ippoliti, Valentina Rovella, Nicola Di Daniele, Erica Giacobbi, Marina Cardellini, Francesca Di Daniele, P. Borboni, Lucia Anemona, Francesca Servadei, and Iacopo Maria Legramante
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Carotid Artery Diseases ,Male ,Time Factors ,Endocrinology, Diabetes and Metabolism ,Settore MED/09 ,030204 cardiovascular system & hematology ,0302 clinical medicine ,Risk Factors ,Original Investigation ,Aged, 80 and over ,Hypertriglyceridemia ,Incidence ,Incidence (epidemiology) ,Middle Aged ,Prognosis ,Metabolic syndrome ,Plaque, Atherosclerotic ,Italy ,Disease Progression ,Cardiology ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,medicine.medical_specialty ,Histology ,Risk Assessment ,Asymptomatic ,03 medical and health sciences ,Sex Factors ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Diseases of the circulatory (Cardiovascular) system ,In patient ,Triglycerides ,Aged ,Retrospective Studies ,Carotid ,Angiology ,Rupture, Spontaneous ,business.industry ,Odds ratio ,medicine.disease ,RC666-701 ,Post‐menopause ,business ,Biomarkers ,030217 neurology & neurosurgery - Abstract
Background Metabolic syndrome certainly favors growth of carotid plaque; however, it is uncertain if it determines plaque destabilization. Furthermore, it is likely that only some components of metabolic syndrome are associated with increased risk of plaque destabilization. Therefore, we evaluated the effect of different elements of metabolic syndrome, individually and in association, on carotid plaques destabilization. Methods A total of 186 carotid endarterectomies from symptomatic and asymptomatic patients were histologically analysed and correlated with major cardiovascular risk factors. Results Metabolic syndrome, regardless of the cluster of its components, is not associated with a significant increase in risk of plaque destabilization, rather with the presence of stable plaques. The incidence of unstable plaques in patients with metabolic syndrome is quite low (43.9 %), when compared with that seen in the presence of some risk factors, but significantly increases in the subgroup of female patients with hypertriglyceridemia, showing an odds ratio of 3.01 (95% CI, 0.25–36.30). Conclusions Our data may help to identify patients with real increased risk of acute cerebrovascular diseases thus supporting the hypothesis that the control of hypertriglyceridemia should be a key point on prevention of carotid atherosclerotic plaque destabilization, especially in post-menopausal female patients.
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- 2021
8. Oral manifestation of indolent T‐cell lymphoproliferative disorder of the gastrointestinal tract: A potential diagnostic pitfall
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Francesca Baciorri, Monia Di Prete, Anna Giulia Zizzari, Lucia Anemona, and Andrea Saggini
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Male ,Pathology ,medicine.medical_specialty ,Gastrointestinal tract ,Histology ,Gastrointestinal Diseases ,business.industry ,Biopsy ,T-Lymphocytes ,T cell ,Remission Induction ,Aftercare ,Dermatology ,CD8-Positive T-Lymphocytes ,medicine.disease ,Lymphoproliferative Disorders ,Peripheral T-cell lymphoma ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,Tongue ,Adrenal Cortex Hormones ,medicine ,Humans ,business ,Aged - Published
- 2020
9. Timp3 deficiency affects the progression of DEN-related hepatocellular carcinoma during diet-induced obesity in mice
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Viviana Casagrande, Mara D'Onofrio, Ivan Arisi, Giulia Iuliani, Alessandro Mauriello, Maria Mavilio, Lorenzo De Angelis, Lucia Anemona, Massimo Federici, and Rossella Menghini
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Male ,medicine.medical_specialty ,Carcinoma, Hepatocellular ,TIMP3 ,Settore MED/09 - Medicina Interna ,Hepatocellular carcinoma ,Endocrinology, Diabetes and Metabolism ,Settore MED/08 ,030209 endocrinology & metabolism ,Inflammation ,Endogeny ,030204 cardiovascular system & hematology ,Diet, High-Fat ,Obesity ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Mediator ,Internal medicine ,Diabetes mellitus ,Internal Medicine ,medicine ,Animals ,Diethylnitrosamine ,Mice, Knockout ,Tissue Inhibitor of Metalloproteinase-3 ,2. Zero hunger ,business.industry ,Forkhead Box Protein M1 ,Liver Neoplasms ,Cancer ,General Medicine ,Tissue inhibitor of metalloproteinase ,medicine.disease ,3. Good health ,Fatty Liver ,Mice, Inbred C57BL ,Disease Progression ,FOXM1 ,medicine.symptom ,business ,Signal Transduction - Abstract
Obesity and low-grade inflammation are associated with an increased risk of hepatocellular carcinoma (HCC), a leading cause of cancer-related death worldwide. The tissue inhibitor of metalloproteinase (TIMP) 3, an endogenous inhibitor of protease activity that represents a key mediator of inflammation, is reduced in inflammatory metabolic disorders and cancer. In contrast, Timp3-deficient mice (Timp3−/−) are highly resistant to developing HCC in response to a diethylnitrosamine (DEN); therefore, we aimed to elucidate the biological role of genetic loss of Timp3 in obesity-related hepatocarcinogenesis. Fourteen-day-old male wild-type (wt) and Timp3−/− mice were injected with 25 mg/kg DEN or an equal volume of saline. After 4 weeks, mice were randomized into two dietary groups and fed either normal or high-fat diet and allowed to grow until 32 weeks of age. Liver histological features were analyzed, and differentially expressed genes in the liver were quantified. In Timp3−/− mice fed with the obesogenic diet, despite the increase in liver steatosis and inflammation, both the number of tumors and the total tumor size are significantly reduced 30 weeks post-DEN injection, compared to control mice. Moreover, Timp3 deletion in hepatocarcinogenesis during obesity is associated with a reduction in FoxM1 transcriptional activity through H19/miR-675/p53 pathway. This study suggests that Timp3 ablation leads to cell cycle perturbation, at least in part by repressing FoxM1 transcriptional activity through H19/miR-675/p53 pathway.
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- 2019
10. Persistence of SARS-CoV-2 Viral RNA in Nasopharyngeal Swabs after Death: An Observational Study
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Orazio Schillaci, Manuela Montanaro, Manuel Scimeca, Lucia Anemona, Erica Giacobbi, Sergio Bernardini, Francesca Servadei, Luigi Tonino Marsella, Nicoletta Urbano, Marco Ciotti, Bartolo Caggiano, Michele Treglia, Alessandro Mauriello, and Silvestro Mauriello
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0301 basic medicine ,Microbiology (medical) ,viruses ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Settore MED/08 ,Autopsy ,Microbiology ,Virus ,Persistence (computer science) ,03 medical and health sciences ,0302 clinical medicine ,autopsy ,Virology ,Medicine ,Viral rna ,030216 legal & forensic medicine ,lcsh:QH301-705.5 ,medico-legal procedures ,biology ,SARS-CoV-2 ,business.industry ,allergology ,Communication ,pandemic ,030104 developmental biology ,lcsh:Biology (General) ,biology.protein ,Observational study ,Antibody ,business ,Viral load - Abstract
Background: The aim of this study was to investigate the persistence of SARS-CoV-2 in post-mortem swabs of subjects who died from SARS-CoV-2 infection. Methods: The presence of the virus was evaluated post-mortem from airways of 27 SARS-CoV-2 positive patients at three different time points (T1 2 hours; T2 12 hours – T3 24 hours) by real-time PCR. Detection of antibodies to SARS-CoV-2 was performed by Maglumi 2019-nCoV IgM/IgG chemiluminescence assay. Results: SARS-CoV-2 viral RNA was still detectable in 70,3% of cases within 2 hours after death and in 66,6% of cases up to 24 hours after death. Our data showed an increase of the viral load in 78,6% of positive individuals 24 hours post-mortem (T3) in comparison to that evaluated 2 hours after death (T1). Noteworthy, we detected a positive T3 post-mortem swab (24 hours after death) from 4 subjects who were negative at T1 (2 hours after death). Conclusion: The results of our study may have an important value in the management of deceased subjects not only with a suspected or confirmed diagnosis of SARS-CoV-2, but also for unspecified causes and in the absence of clinical documentation or medical assistance.
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- 2021
11. New immunological potential markers for triple negative breast cancer: IL18R1, CD53, TRIM, Jaw1, LTB, PTPRCAP
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Paolo Marchetti, Gerry Melino, Alexey Antonov, Andrea Botticelli, M. Valeria Catani, Manuela Montanaro, Lucia Anemona, Chaitania Vangapandou, Alessandro Mauriello, Marchetti, Paolo [0000-0001-5170-9579], Anemona, Lucia [0000-0002-3711-2714], Botticelli, Andrea [0000-0002-6425-9893], Mauriello, Alessandro [0000-0002-7351-5676], Melino, Gerry [0000-0001-9428-5972], Catani, M. Valeria [0000-0002-7088-9242], Apollo - University of Cambridge Repository, and Catani, M Valeria [0000-0002-7088-9242]
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0301 basic medicine ,Cancer Research ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Cancer immunity ,Jaw1 ,Prognostic markers ,03 medical and health sciences ,TRIM ,0302 clinical medicine ,Endocrinology ,Immune system ,Breast cancer ,cancer immunity ,cd53 ,il18r1 ,jaw1 ,ltb ,precision oncology ,prognostic markers ,ptprcap ,trim ,triple negative breast cancer ,Surgical oncology ,medicine ,Triple negative breast cancer ,Settore BIO/10 ,IL18R1 ,Triple-negative breast cancer ,Endocrine and Autonomic Systems ,Genetic heterogeneity ,business.industry ,Research ,Precision oncology ,PTPRCAP ,medicine.disease ,Molecular medicine ,Radiation therapy ,030104 developmental biology ,Oncology ,LTB ,030220 oncology & carcinogenesis ,Cancer cell ,Cancer research ,CD53 ,business - Abstract
Breast cancer (BC) is the second leading cause of cancer death in women worldwide, and settings of specific prognostic factors and efficacious therapies are made difficult by phenotypic heterogeneity of BC subtypes. Therefore, there is a current urgent need to define novel predictive genetic predictors that may be useful for stratifying patients with distinct prognostic outcomes. Here, we looked for novel molecular signatures for triple negative breast cancers (TNBCs). By a bioinformatic approach, we identified a panel of genes, whose expression was positively correlated with disease-free survival in TNBC patients, namely IL18R1, CD53, TRIM, Jaw1, LTB, and PTPRCAP, showing specific immune expression profiles linked to survival prediction; most of these genes are indeed expressed in immune cells and are required for productive lymphocyte activation. According to our hypothesis, these genes were not, or poorly, expressed in different TNBC cell lines, derived from either primary breast tumours or metastatic pleural effusions. This conclusion was further supported in vivo, as immuno-histochemical analysis on biopsies of TNBC invasive ductal carcinomas highlighted differential expression of these six genes in cancer cells, as well as in intra- and peri-tumoral infiltrating lymphocytes. Our data open to the possibility that inter-tumour heterogeneity of immune markers might have predictive value; further investigations are recommended in order to establish the real power of cancer-related immune profiles as prognostic factors. Supplementary Information The online version contains supplementary material available at 10.1007/s12672-021-00401-0.
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- 2021
12. Case Report: Early Breast Cancer Recurrence Mimicking BIA-ALCL in a Patient With Multiple Breast Procedures
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Rosaria Meucci, Andrea Farinaccio, Gianluca Vanni, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Francesca Santori, Amir Sadri, Valentina Bruno, Marcello Chiocchi, Francesca Servadei, Federico Tacconi, Marco Pellicciaro, Lucia Anemona, Camilla Di Pasquali, Marco Materazzo, Erika Giacobbi, Tommaso Perretta, Adriano De Majo, and Maria Cotesta
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medicine.medical_specialty ,locoregional recurrence ,medicine.medical_treatment ,lcsh:Surgery ,Breast procedures ,residual breast tissue ,Disease ,030230 surgery ,breast seroma ,law.invention ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,breast cancer ,law ,hemic and lymphatic diseases ,medicine ,case report ,skin and connective tissue diseases ,medicine.diagnostic_test ,business.industry ,Large-cell lymphoma ,lcsh:RD1-811 ,immediate breast reconstruction ,medicine.disease ,Fine-needle aspiration ,macro textured breast implants ,breast implant associated-anaplastic large cell lymphoma ,030220 oncology & carcinogenesis ,Breast implant ,Surgery ,Radiology ,Breast reconstruction ,business ,Mastectomy - Abstract
Breast reconstruction plays a fundamental role in the therapeutic process of breast cancer treatment and breast implants represents the leading breast reconstruction strategy. Breast Implant Associated-Anaplastic Large Cell Lymphoma (BIA-ALCL), locoregional recurrence in the skin flap, and skin flap necrosis are well-known complications following mastectomy and immediate breast reconstruction (IBR). We report a case of locoregional cancer recurrence in the mastectomy flap mimicking BIA-ALCL, in a patient who underwent 6 breast procedures in four facilities across 15 years including immediate breast reconstruction with macrotextured breast implants. Despite the rate and onset of the disease, clinicians should be aware of BIA-ALCL. Due to the risk of false negative results of fine needle aspiration, clinical suspicion of BIA-ALCL should drive clinicians' choices, aside from cytological results. In the present case, surgical capsulectomy of the abnormal periprosthesic tissue revealed locoregional recurrence.
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- 2021
13. Unilateral Maculopapular Eruption in a Child: Challenge
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Andrea Saggini, Maria Vittoria Cannizzaro, Luca Bianchi, Lucia Anemona, and Augusto Orlandi
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medicine.medical_specialty ,Text mining ,Settore MED/35 ,business.industry ,medicine ,Dermatology ,General Medicine ,business ,Pathology and Forensic Medicine - Published
- 2021
14. Persistence of SARS-CoV-2 viral RNA in nasopharyngeal swabs after death
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Manuel Scimeca, Michele Treglia, Silvestro Mauriello, Orazio Schillaci, Bartolo Caggiano, Alessandro Mauriello, Manuela Montanaro, Erica Giacobbi, Lucia Anemona, Luigi Tonino Marsella, Sergio Bernardini, Marco Ciotti, and Francesca Servadei
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Microbiology (medical) ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Reverse Transcriptase Polymerase Chain Reaction ,SARS-CoV-2 ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,RNA ,COVID-19 ,Viral Load ,Virology ,Persistence (computer science) ,Infectious Diseases ,Viral genetics ,Nasopharynx ,Quarantine ,Settore MED/43 ,Medicine ,Humans ,RNA, Viral ,Viral rna ,business ,Letter to the Editor - Published
- 2021
15. Juan Rosai as master of our comprehensive understanding of thymus and thymoma
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Hans Konrad Müller-Hermelink, Alexander Marx, Mirella Marino, Lucia Anemona, Libero Lauriola, and Philipp Ströbel
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Male ,Pathology ,medicine.medical_specialty ,Thymoma ,Thymic Tumors ,Settore MED/08 ,Review ,Diagnostic tools ,World health ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,thymus ,Disease patterns ,medicine ,Humans ,Neoplasms, Glandular and Epithelial ,Thymic carcinoma ,030304 developmental biology ,0303 health sciences ,business.industry ,Mediastinum ,Thymus Neoplasms ,medicine.disease ,Immunohistochemistry ,3. Good health ,medicine.anatomical_structure ,classification ,030220 oncology & carcinogenesis ,pathology ,business ,Mediastinal Diseases - Abstract
Summary In this study, the authors report on the activity of Juan Rosai, one of the pathologists most engaged in the definition of cells, diseases and tumors occurring in the thymus and in the mediastinum during the last 60 years. With his morphological skills and tireless interest in clarification of disease patterns, he contributed extraordinarily to expand our knowledge of the mediastinal diseases and to improve our diagnostic approach. He determined extraordinary advances also in trasmission electron microscopy and in immunohistochemistry as powerful diagnostic tools. Moreover, he proposed and promoted, together with an international panel of Pathologists, the World Health Classification of Thymic tumors as a definite progress in our comprehension and diagnostics of thymic epithelial tumors (TET). Our purpose is to review J. Rosai’s achievements in thymic normal structure, in TET and particularly in the entity now definied as “thymoma”, in distinction from the thymic carcinoma. To do this, our narrative will also be based on personal memories, longstanding collaborations and/or friendship with J. Rosai.
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- 2021
16. Breast Trauma and Triple-Negative Hemorrhagic Cystic Carcinoma: Management and Treatment
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Maria Cotesta, Erica Giacobbi, Alessandra Vittoria Granai, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Francesca Servadei, Susanna Finocchiaro, Marco Pellicciaro, Lucia Anemona, Francesca Santori, Adriano De Majo, Marco Materazzo, and Gianluca Vanni
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medicine.medical_specialty ,medicine.medical_treatment ,Breast Neoplasms ,Triple Negative Breast Neoplasms ,030204 cardiovascular system & hematology ,Modified Radical Mastectomy ,Breast cysts ,03 medical and health sciences ,0302 clinical medicine ,Breast Cyst ,Biopsy ,Carcinoma ,Humans ,Medicine ,Cyst ,Mastectomy ,medicine.diagnostic_test ,Cysts ,business.industry ,Cancer ,Articles ,General Medicine ,Middle Aged ,medicine.disease ,030220 oncology & carcinogenesis ,Female ,Histopathology ,Radiology ,Neoplasm Recurrence, Local ,business - Abstract
Patient: Female, 59-year-old Final Diagnosis: Invasive breast carcinomas of no special type (NST) Symptoms: Recurrent hemorrhagic cystic lesion Medication: — Clinical Procedure: — Specialty: Surgery Objective: Challenging differential diagnosis Background: Breast trauma can always have diagnostic pitfalls. In the presence of a recurrent hemorrhagic cysts, cancer should always be suspected. Case Report: A 59-year-old woman noted a palpable mass after breast trauma from falling at home. Radiological exams showed a breast cyst with well-defined margins, with corpuscular and dense fluid components. First, a conservative approach was implemented. One week later, a fine-needle aspiration cytology (FNAC) of the mass showed bloody fluid without atypical cells. Three weeks later, the patient was emergently evaluated due to increased size of the lesion and anemia. To avoid further blood loss and due to suspected malignancy, an urgent surgical excision biopsy was planned. Histopathology revealed a poorly-differentiated carcinoma and the patient was treated with left modified radical mastectomy with axillary dissection. Adjuvant chemotherapy was administered. At 6-month follow-up, the patient was free from recurrences. Conclusions: Recurrent hemorrhagic cysts should always be investigated and considered as a possible cancer lesion. Sonography and cytological exam are the first steps in case of suspicious cysts, but false-negative results are common. In such cases, resection of the cyst should be considered. Immediate resection is valid in cases of diagnostic uncertainty or inability to assess the cyst with imaging or biopsy.
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- 2020
17. Effects of Risk Factors on In Situ Expression of Proinflammatory Markers Correlated to Carotid Plaque Instability
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Manuela Montanaro, Nicola Toschi, Francesca Servadei, Arnaldo Ippoliti, Lucia Anemona, Alessandro Mauriello, Elena Bonanno, Manuel Scimeca, Giuseppe Santeusanio, and Erica Giacobbi
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Carotid Artery Diseases ,Male ,Pathology ,medicine.medical_specialty ,Histology ,Asymptomatic ,Pathology and Forensic Medicine ,Proinflammatory cytokine ,Sex Factors ,Antigens, CD ,Risk Factors ,medicine ,Humans ,Stroke ,Aged ,Aged, 80 and over ,Tissue microarray ,business.industry ,Interleukin ,Middle Aged ,medicine.disease ,Plaque, Atherosclerotic ,Settore MED/22 ,Medical Laboratory Technology ,Carotid Arteries ,Gene Expression Regulation ,Immunohistochemistry ,Cytokines ,Female ,medicine.symptom ,business ,CD163 ,CD8 ,Biomarkers - Abstract
BACKGROUND AND AIMS Several studies demonstrated a role of active chronic inflammatory infiltrate in carotid plaques progression suggesting a possible link between cardiovascular risk factors and inflammation-related plaque instability. The aim of this study is therefore to evaluate the possible effects of cardiovascular risk factors on in situ expression of proinflammatory markers associated with carotid plaque instability. METHODS AND RESULTS A tissue microarray containing carotid plaques from 36 symptomatic (major stroke or transient ischemic attack) and 37 asymptomatic patients was built. Serial sections were employed to evaluate the expression of some inflammatory markers by immunohistochemistry [CD3, CD4a, CD8, CD20, CD86, CD163, interleukin (IL)-2, IL-6, IL-17]. Immunohistochemical data were analyzed to study the possible associations between in situ expression of inflammatory biomarker and the main cardiovascular risk factors. Our data demonstrated that plaque instability is associated with the high in situ expression of some cytokines, such as IL-2, IL-6, IL-17. Besides the female sex, none of the risk factors analyzed showed a significant association between the in situ expression of these markers and unstable plaques. A significant increase of IL-6-positive and IL-17-positive cells was observed in unstable atheromatous plaques of female patients, as compared with unstable plaques of male patients. CONCLUSIONS Plaque destabilization is certainly correlated with the presence of the major cardiovascular risk factors, however, our results showed that, with the exception of sex, their action in the evolutive process of plaque instability seems rather nonspecific, favoring a general release of proinflammatory cytokines.
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- 2020
18. Colonic Adenoma with Chronic Lymphocytic Leukemia Infiltration
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B. Neri, M. Giannelli, Federico Meconi, Lucia Anemona, and Marco Pocci
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Pathology ,medicine.medical_specialty ,Adenoma ,business.industry ,Chronic lymphocytic leukemia ,Colonic Adenoma ,Gastroenterology ,Medicine ,business ,medicine.disease ,Infiltration (medical) - Published
- 2020
19. Evaluation of Concordance Between Histopathological, Radiological and Biomolecular Variables in Breast Cancer Neoadjuvant Treatment
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Ilaria Portarena, Rosaria Meucci, Ljuba Morando, Camilla Deiana, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Lucia Anemona, Andrea Grasso, Gianluca Vanni, and Marco Materazzo
- Subjects
Oncology ,Cancer Research ,medicine.medical_specialty ,medicine.medical_treatment ,Concordance ,Breast Neoplasms ,Disease-Free Survival ,Immunophenotyping ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Settore MED/36 ,Neoadjuvant treatment ,Internal medicine ,Receptors ,medicine ,Humans ,complete pathological response ,skin and connective tissue diseases ,Progesterone ,Histological examination ,Chemotherapy ,business.industry ,histological examination ,General Medicine ,Gold standard (test) ,Middle Aged ,medicine.disease ,MRI accuracy ,Estrogen ,Neoadjuvant Therapy ,Ki-67 Antigen ,Receptors, Estrogen ,neoadjuvant chemotherapy ,Female ,Receptors, Progesterone ,030220 oncology & carcinogenesis ,Radiological weapon ,Immunohistochemistry ,business - Abstract
Background/aim Neoadjuvant chemotherapy (NAC) for breast cancer (BC) is the gold standard treatment for locally advanced tumors (LABC) that aims at achieving a complete pathological response (pCR). Studies have been conducted to evaluate and identify te concordance between radiological, histopathological and biological variables of BC and final response to therapy, verified by definitive histological examination after surgery. Patients and methods Ninety-five BC patients were examined and subjected to NAC. Immunohistochemical markers including oestrogen-receptor (ER), progesterone-receptor (PR), Ki67 index, and human epidermal growth factor receptor 2 (HER2) score were examined before and after neoadjuvant treatment. Results Younger age and a significant decrease in ER expression were associated with better prognosis. Triple Negative (TN) and Her2-type breast cancers benefited most from neoadjuvant chemotherapy with higher frequency of pCR. Conclusion HER2-type and TN BC are correlated with best response to NAC. A statistically significant correlation between radiological images and definitive histological examination was not observed.
- Published
- 2020
20. Reducing the Number of Unnecessary Percutaneous Biopsies: The Role of Second Opinion by Expert Breast Center Radiologists
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Alberto Collura, Daniela Tosti, Feliciana Lamacchia, Francesca Ricci, Marco Materazzo, Michela Censi, Gianluca Vanni, Tommaso Perretta, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Francesca Di Giuliano, and Lucia Anemona
- Subjects
Core needle ,Data Analysis ,Image-Guided Biopsy ,Cancer Research ,medicine.medical_specialty ,Percutaneous ,Biopsy ,Biopsy, Fine-Needle ,Clinical Decision-Making ,breast imaging reporting and data system ,Breast Neoplasms ,Fine needle aspiration cytology ,breast lesions ,Tru-Cut needle biopsy ,breast cancer ,cancer ,multidisciplinary team ,tertiary care center ,Biopsy, Large-Core Needle ,Disease Management ,Expert Testimony ,Female ,Humans ,Mammography ,Radiologists ,Referral and Consultation ,Workflow ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,Settore MED/36 ,medicine ,Family history ,skin and connective tissue diseases ,medicine.diagnostic_test ,business.industry ,Second opinion ,Cancer ,General Medicine ,medicine.disease ,Fine-needle aspiration ,Oncology ,030220 oncology & carcinogenesis ,Fine-Needle ,Large-Core Needle ,Radiology ,business - Abstract
BACKGROUND/AIM The research objectives of this study were the estimation of the number of misdiagnosed breast lesions by non-expert-center-breast-radiologists (NEBR) and the investigation of the discordant rate (DR) calculated between initial and second opinion. Moreover, this study evaluated the impact of second opinion and the factors associated with DR. MATERIALS AND METHODS A total of 399 patients were sent to our Tertiary Breast Cancer (BC) Center to perform fine needle aspiration/core needle biopsy (FNAC/CNB) after external examination. Lesions were reclassified according to Breast Imaging-Reporting and Data System (BI-RADS). External examinations were classified as breast-expert, not-breast-expert and physicians as expert-center-breast-radiologists (EBR), NEBR, and non-radiologists (NR). Personal/family history of breast cancer (BC), breast-density and presence of prior imaging were collected. RESULTS DR was 74.3%. After second opinion, FNAC/CNB was proposed in 25.7% of cases and 2 additional cancers were detected. About 59.5% of unnecessary FNAC/CNB were avoided. Dense breast, no prior imaging examination and BC family-history were associated with higher DR (p-value
- Published
- 2019
21. Unilateral Maculopapular Eruption in a Child: Answer
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Maria Vittoria Cannizzaro, Andrea Saggini, Luca Bianchi, Augusto Orlandi, and Lucia Anemona
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medicine.medical_specialty ,Settore MED/35 ,Text mining ,business.industry ,medicine ,Dermatology ,General Medicine ,business ,Pathology and Forensic Medicine - Published
- 2021
22. Histological features of bone marrow in paediatric patients during the asymptomatic phase of early-stage Black African sickle cell anaemia
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Simone Facchetti, Antonella Isgrò, Alessandro Mauriello, Lucia Anemona, Andrea Saggini, and Erica Giacobbi
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Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Myeloid ,Adolescent ,Settore MED/08 ,Bone Marrow Cells ,Anemia, Sickle Cell ,Asymptomatic ,Pathology and Forensic Medicine ,Sickle cell anaemia ,electron microscopy ,pathology ,Bone Marrow ,Child ,Child, Preschool ,Female ,Hematopoietic Stem Cell Transplantation ,Humans ,Myelodysplastic Syndromes ,Spleen ,03 medical and health sciences ,medicine ,Preschool ,business.industry ,Anemia ,Erythroid Hyperplasia ,Pathophysiology ,Sickle Cell ,Transplantation ,Haematopoiesis ,030104 developmental biology ,medicine.anatomical_structure ,Bone marrow ,Stem cell ,medicine.symptom ,business - Abstract
Bone marrow histological features of sickle cell anaemia (SCA) patients during early stages and in the asymptomatic phase of the disease appear an interesting area of study, representing early-stage consequences of SCA with a close relation to its pathophysiology. Unfortunately, this field of research has never been specifically addressed before. Bone marrow biopsies from 26 consecutive Black African SCA patients (M:F=1.6:1; age 2-17 years), free of clinical signs of chronic bone marrow damage, with no recent history of symptomatic vaso-occlusive episodes, and waiting for haematopoietic stem cell transplantation (HSCT), underwent morphological, immunohistochemical and electron microscopy evaluation. Additional comparison with three bone marrow specimens from post-HSCT SCA patients and 10 bone marrow specimens from AS healthy carriers was performed. Bone marrow of SCA patients was normocellular or slighly hypercellular in all cases. Erythroid hyperplasia was a common feature. Myeloid lineage was slightly decreased with normal to slightly diminished neutrophilic granulocytes; CD68 positive monocytic-macrophagic cells appeared slightly increased, with a predominant CD163 positive M2/M(Hb) phenotype. A positive correlation was found between haemoglobin values and number of bone marrow erythroid cells (R2=0.15, p=0.05). Intravascular and interstitial clusters of erythroid sickle cells were found in bone marrow of pre-HSCT homozygous SS SCA patients, as well as heterozygous AS healthy carriers, and the single post-HSCT patient matched to an AS health carrier donor.
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- 2017
23. p63 Is a Promising Marker in the Diagnosis of Unusual Skin Cancer
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Artem Smirnov, Eleonora Candi, Flavia Novelli, Margherita Annicchiarico-Petruzzelli, Lucia Anemona, Cristina M. Piro, and Gerry Melino
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0301 basic medicine ,Oncology ,squamous cell carcinoma ,medicine.medical_specialty ,Skin Neoplasms ,Early detection ,Review ,Catalysis ,lcsh:Chemistry ,Inorganic Chemistry ,03 medical and health sciences ,0302 clinical medicine ,Merkel cell carcinoma ,basal cell carcinoma ,Internal medicine ,medicine ,Biomarkers, Tumor ,Humans ,Basal cell carcinoma ,Settore BIO/10 ,Physical and Theoretical Chemistry ,lcsh:QH301-705.5 ,Molecular Biology ,Melanoma ,Spectroscopy ,p63 ,integumentary system ,skin cancer ,business.industry ,Tumor Suppressor Proteins ,Organic Chemistry ,p40 ,Cancer ,General Medicine ,medicine.disease ,Prognosis ,Immunohistochemistry ,Computer Science Applications ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,Carcinoma, Basal Cell ,030220 oncology & carcinogenesis ,Carcinoma, Squamous Cell ,Skin cancer ,business ,Transcription Factors - Abstract
Skin cancer is the most common type of cancer worldwide. Ozone depletion and climate changes might cause a further increase in the incidence rate in the future. Although the early detection of skin cancer enables it to be treated successfully, some tumours can evolve and become more aggressive, especially in the case of melanoma. Therefore, good diagnostic and prognostic markers are needed to ensure correct detection and treatment. Transcription factor p63, a member of the p53 family of proteins, plays an essential role in the development of stratified epithelia such as skin. In this paper, we conduct a comprehensive review of p63 expression in different types of skin cancer and discuss its possible use in the diagnosis and prognosis of cutaneous tumours.
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- 2019
24. The Efficacy and Safety of Lenalidomide plus Rituximab in an Orbital Relapse of Diffuse Large B-Cell Lymphoma
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G. Paterno, Lucia Anemona, Federico Meconi, Daniela Nasso, Vito Mario Rapisarda, Sara Vaccarini, Maria Cantonetti, Livio Pupo, Fabiana Esposito, and Raffaele Palmieri
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Oncology ,medicine.medical_specialty ,medicine.medical_treatment ,Case Report ,03 medical and health sciences ,0302 clinical medicine ,Maintenance therapy ,Blurred vision ,Internal medicine ,hemic and lymphatic diseases ,medicine ,Stage (cooking) ,Lenalidomide ,lcsh:RC633-647.5 ,business.industry ,lcsh:Diseases of the blood and blood-forming organs ,General Medicine ,Settore MED/15 ,medicine.disease ,Lymphoma ,Radiation therapy ,030220 oncology & carcinogenesis ,Rituximab ,medicine.symptom ,business ,Diffuse large B-cell lymphoma ,030215 immunology ,medicine.drug - Abstract
A 74-year-old male with diffuse large B-cell lymphoma, with an Ann Arbor stage IV-A, was submitted to immune-chemotherapy in 2014, with complete remission of the disease. Two years later, he presented with a left eye swelling leading to exophthalmos and blurred vision. A core biopsy was performed and revealed a local relapse of the disease. He was considered unfit for intensive salvage chemotherapy and was treated with a combination of rituximab and lenalidomide. After six courses of rituximab plus lenalidomide, the patient showed complete remission and was submitted to maintenance therapy with lenalidomide. After 24 months since the start of lenalidomide monotherapy, we did not observe any progression. In this experience, rituximab plus lenalidomide, without radiotherapy, was a safe and effective therapeutic combination in an elderly patient with a localized relapse of DLBCL who was unfit to receive more aggressive therapies.
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- 2019
25. Morphological evaluation of sympathetic renal innervation in patients with autosomal dominant polycystic kidney disease
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Nicola Di Daniele, Alessandro Mauriello, Adriana D'Ercole, Pierluigi Bove, Giuseppe Santeusanio, Silvestro Mauriello, Annalisa Noce, Elena Bonanno, Erica Giacobbi, Manuel Scimeca, Gabriele D'Urso, Alessandro Anselmo, Maurizio Casasco, Valentina Rovella, Lucia Anemona, and Elena Giannini
- Subjects
ADPKD ,Histology ,Renal denervation ,Sympathetic renal hyperactivity ,Adult ,Male ,medicine.medical_specialty ,Adventitia ,Sympathetic Nervous System ,medicine.medical_treatment ,030232 urology & nephrology ,Urology ,Autosomal dominant polycystic kidney disease ,Kidney Volume ,Settore MED/08 - Anatomia Patologica ,030204 cardiovascular system & hematology ,urologic and male genital diseases ,Nephrectomy ,03 medical and health sciences ,0302 clinical medicine ,Renal Artery ,medicine.artery ,medicine ,Humans ,In patient ,Renal artery ,Aged ,Aged, 80 and over ,Kidney ,business.industry ,Middle Aged ,medicine.disease ,Polycystic Kidney, Autosomal Dominant ,medicine.anatomical_structure ,Nephrology ,Case-Control Studies ,Hypertension ,Immunohistochemistry ,Female ,business - Abstract
Several evidences support the hypothesis that patients affected by autosomal dominant polycystic kidney disease (ASPKD) show a sympathetic renal hyperactivity. Nevertheless, no morphological evidences are available yet. Therefore, the aim of the study was to demonstrate that an increase in sympathetic renal artery innervation was present in the ADPKD patients by using histological methods. In addition, here we correlated the sympathetic renal artery innervation with the evolutionary state of ADPKD (increase in volume of kidney, onset of chronic renal failure and hypertension). To this end, peri-adventitial innervation of renal arteries was studied using morphological methods from 49 patients in total: 29 underwent surgical nephrectomies for ADPKD and 20 non-dialysis patients (CTRL group) undergoing nephrectomy for other diseases. Nerve density (number of nerves per mm2) was evaluated in the peri-adventitial tissue in a concentric ring that was located within 2 mm from the beginning of the adventitia by using immunohistochemistry. The total nerve density was significantly increased in the ADPKD group (1.26 ± 0.82 × mm2) as compared to controls (0.78 ± 0.40 × mm2) (p = 0.02). Hypertensive patients with ADPKD showed a greater nerve density than control hypertensives. However, the increase in renal sympathetic innervation in the ADPKD patients was found to be independent of hypertension, resistance to antihypertensive therapy, age, sex and kidney volume, as demonstrated by the uni and multivariate analysis. In conclusion, our study better clarifies the effect of sympathetic hyperactivity in the progression of polycystic disease.
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- 2019
26. Chronic kidney disease is linked to carotid nodular calcification, an unstable plaque not correlated to inflammation
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Andrea Saggini, Giuseppe Santeusanio, Massimo Federici, Manuel Scimeca, Lucia Anemona, Francesca Davato, Arnaldo Ippoliti, Marina Cardellini, Marta Ballanti, Simone Bischetti, Elena Bonanno, Rossella Menghini, Valentina Rovella, Sara Casella, Alessandro Mauriello, and Nicola Di Daniele
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Pathology ,Settore MED/09 - Medicina Interna ,medicine.medical_treatment ,carotid, nodular calcification,chronic kidney disease, histopathology ,Settore MED/08 ,Settore MED/09 ,Asymptomatic ,Orginal Article ,Settore MED/22 - Chirurgia Vascolare ,Pathology and Forensic Medicine ,Pathogenesis ,03 medical and health sciences ,carotid ,chronic kidney disease ,histopathology ,nodular calcification ,0302 clinical medicine ,Diabetes mellitus ,medicine ,Clinical significance ,Endarterectomy ,business.industry ,Cell Biology ,medicine.disease ,030104 developmental biology ,Histopathology ,Neurology (clinical) ,Geriatrics and Gerontology ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Kidney disease ,Calcification - Abstract
The incidence and the different type of carotid calcifications, nodular and non-nodular, and their role in the acute cerebrovascular disease has not yet been defined. Various studies have correlated the presence of specific risk factors, in particular the chronic kidney disease, with the presence of calcification, but not with the type of calcification. Since it is likely that carotid nodular calcifications rather than those with non-nodular aspect may represent a plaque at high risk of rupture, the purpose of our study was to evaluate the role of nodular calcification in the pathogenesis of cerebrovascular syndromes and their possible correlation with specific risk factors. A total of 168 carotid plaques from symptomatic and asymptomatic patients submitted to endarterectomy, whom complete clinical and laboratory assessment of major cardiovascular risk factors was available, were studied. In 21 endarterectomies (5 from symptomatic and 16 from asymptomatic patients) an eruptive calcified nodule, consisting of calcified plates associated to a small amount of fibrous tissue without extracellular lipids and inflammatory cells, was found protruding into the lumen. Nodular calcifications were significantly observed in patients affected by chronic kidney disease (with GFR
- Published
- 2019
27. Transglutaminase 3 is expressed in basal cell carcinoma of the skin
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Manuela Montanaro, Lucia Anemona, Elena Campione, Gerry Melino, Margherita Annicchiarico-Petruzzelli, Artem Smirnov, Eleonora Candi, and Alessandro Mauriello
- Subjects
0301 basic medicine ,Skin Neoplasms ,Tissue transglutaminase ,Protein Array Analysis ,Fluorescent Antibody Technique ,Settore MED/08 ,Dermatology ,03 medical and health sciences ,0302 clinical medicine ,Biomarkers, Tumor ,medicine ,Humans ,Basal cell carcinoma ,RNA, Messenger ,Settore BIO/10 ,Microscopy, Confocal ,Transglutaminases ,integumentary system ,biology ,business.industry ,Settore BIO/11 ,Melanoma ,medicine.disease ,Staining ,030104 developmental biology ,medicine.anatomical_structure ,Carcinoma, Basal Cell ,Cytoplasm ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Immunohistochemistry ,Epidermis ,Skin cancer ,business - Abstract
Transglutaminase 3 (TG3) belongs to a family of Ca2+-dependent enzymes which catalyse protein crosslinking. TG3 is important for proper development of the skin and hair shaft, and knock-out mice for the Tgm3 gene are sensitive to UVB-induced photodamage due to aberrations in cornified envelope formation. Loss of TG3 is reported in head and neck and oesophageal squamous cell carcinoma, yet, its expression in skin cancer has not been studied. The aim of the present study was to analyse the expression pattern of TG3 in skin cancer. TG3 expression was investigated based on immunohistochemical staining of a tissue micro-array of different types of skin cancer, as well as meta-analysis of public gene array data. Our findings demonstrated that TG3 is normally expressed in spinous/granular layers of the epidermis, but is absent in melanocytes as well as melanoma samples. As expected, its expression was absent in poorly differentiated squamous cell carcinoma of the skin. Surprisingly, we show that samples of basal cell carcinoma demonstrated strong staining for TG3 both in the cytoplasm and nucleus. Furthermore, at the mRNA level, the expression pattern of TGM3 was crucially altered in BCC, but not other types of skin cancer. These findings lead to new questions regarding TG3 involvement in basal cell carcinoma tumourigenesis. Moreover, the expression pattern of TG3 renders it a potential specific marker for basal cell carcinoma diagnosis.
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- 2019
28. Prostate cancer and inflammation: A new molecular imaging challenge in the era of personalized medicine
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Virginia Tancredi, Elena Bonanno, Agostino Chiaravalloti, Donata Trivigno, Rita Bonfiglio, Alessandro Mauriello, Nicoletta Urbano, Lucia Anemona, Manuel Scimeca, Simone Facchetti, Giuseppe Santeusanio, and Orazio Schillaci
- Subjects
Oncology ,Male ,Cancer Research ,medicine.medical_specialty ,Molecular imaging ,Context (language use) ,Settore MED/08 - Anatomia Patologica ,030218 nuclear medicine & medical imaging ,Management of prostate cancer ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Settore MED/36 - Diagnostica per Immagini e Radioterapia ,Prostate ,Internal medicine ,medicine ,Pathology ,Humans ,Radiology, Nuclear Medicine and imaging ,Precision Medicine ,Inflammation ,business.industry ,Cancer ,Prostatic Neoplasms ,Anatomical pathology ,medicine.disease ,Prognosis ,Personalized medicine ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Positron-Emission Tomography ,Molecular Medicine ,Biomarkers ,business - Abstract
The relationship between cancer and inflammation is one of the most important fields for both clinical and translational research. Despite numerous studies reported interesting and solid data about the prognostic value of the presence of inflammatory infiltrate in cancers, the biological role of inflammation in prostate cancer development is not yet fully clarified. The characterization of molecular pathways that connect altered inflammatory response and prostate cancer progression can provide the scientific rationale for the identification of new prognostic and predictive biomarkers. Specifically, the detection of infiltrating immune cells or related-cytokines by histology and/or by molecular imaging techniques could profoundly change the management of prostate cancer patients. In this context, the anatomic pathology and imaging diagnostic teamwork can provide a valuable support for the validation of new targets for diagnosis and therapy of prostate cancer lesions associated to the inflammatory infiltrate. The aim of this review is to summarize the current literature about the role of molecular imaging technique and anatomic pathology in the study of the mutual interaction occurring between prostate cancer and inflammation. Specifically, we reported the more recent advances in molecular imaging and histological methods for the early detection of prostate lesions associated to the inflammatory infiltrate.
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- 2019
29. Plaque calcification is driven by different mechanisms of mineralization associated with specific cardiovascular risk factors
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Silvestro Mauriello, Manuel Scimeca, Annarita Granaglia, Rita Bonfiglio, Alessandro Mauriello, Giuseppe Santeusanio, Orazio Schillaci, Nicoletta Urbano, Elena Bonanno, Nicola Toschi, Stefania Schiaroli, Virginia Tancredi, and Lucia Anemona
- Subjects
Male ,Pathology ,medicine.medical_specialty ,Mineralization ,Endocrinology, Diabetes and Metabolism ,Settore MED/08 ,Medicine (miscellaneous) ,030209 endocrinology & metabolism ,Inflammation ,030204 cardiovascular system & hematology ,Calcitriol receptor ,Atherosclerosis ,BMP-2 ,Plaque calcification ,Risk factors ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Risk Factors ,medicine ,Humans ,Carotid Stenosis ,Osteopontin ,Vascular Calcification ,Aged ,Aged, 80 and over ,Nutrition and Dietetics ,Tissue microarray ,biology ,business.industry ,Settore FIS/07 ,Hypertriglyceridemia ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Plaque, Atherosclerotic ,Carotid Arteries ,chemistry ,RANKL ,Tissue Array Analysis ,biology.protein ,Sclerostin ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,business ,Biomarkers ,Calcification - Abstract
Background and aims The aim of this study was to investigate possible associations among markers of mineralization, plaque instability and the main risk factors of atherosclerosis. Methods and results A Tissue MicroArray containing 52 samples of calcified carotid plaques from 52 symptomatic and asymptomatic patients were built. TMA serial sections were used to study the expression of inflammatory and mineralization markers (BMP-2, BMP-4, VDR, RANKL, Osteopontin, Sclerostin, β-catenin and calmodulin) by immunohistochemistry. Our data clearly demonstrated the expression of mineralization markers in atheromatic plaques. Indeed, with the exception of RANKL, all investigated markers were expressed in at least 60% of cases. Specifically, multivariate analysis displayed significant associations between both the expression of BMP-2 and the presence of unstable plaques as well as between the expression of β-catenin and the presence of stable plaques. We also found a significant inverse association between both a) the presence of hypertension and VDR and b) smoking habits and calmodulin expression. Finally, we noted a higher density of RANKL positive cells in plaques from diabetic patients as compared to non-diabetic ones and a significant positive association between hypertriglyceridemia and BMP-4 expression. Conclusion Our results support the hypothesis that the process of atherosclerotic plaque calcification presents a number of similarities with the physiological processes that occur in bone, involving both osteoblasts- and osteoclasts-like arterial cells. Finally, the present study suggests that risk factors, such as hypertension, cigarette smoke and diabetes, can cause the destabilization of the atheromatic plaque acting on calcification process as well as inflammation.
- Published
- 2018
30. The Paradox Effect of Calcification in Carotid Atherosclerosis: Microcalcification Is Correlated with Plaque Instability
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Elena Bonanno, Francesca Servadei, Erica Giacobbi, Manuela Montanaro, Giuseppe Santeusanio, Arnaldo Ippoliti, Manuel Scimeca, Lucia Anemona, Alessandro Mauriello, Rita Bonfiglio, Orazio Schillaci, and Nicoletta Urbano
- Subjects
Carotid Artery Diseases ,Male ,0301 basic medicine ,Carotid atherosclerosis ,Pathology ,carotid plaque ,030204 cardiovascular system & hematology ,lcsh:Chemistry ,0302 clinical medicine ,Risk Factors ,lcsh:QH301-705.5 ,Spectroscopy ,plaque instability ,macrocalcification ,General Medicine ,Middle Aged ,Immunohistochemistry ,Inflammatory biomarkers ,Plaque, Atherosclerotic ,Computer Science Applications ,Carotid Arteries ,Female ,Microcalcification ,medicine.symptom ,medicine.medical_specialty ,Plaque instability ,macrophage polarization ,Macrophage polarization ,Asymptomatic ,Article ,Catalysis ,Inorganic Chemistry ,03 medical and health sciences ,medicine ,Humans ,Physical and Theoretical Chemistry ,Vascular Calcification ,Molecular Biology ,Aged ,inflammatory biomarkers ,Inflammation ,business.industry ,Macrophages ,Organic Chemistry ,medicine.disease ,Settore MED/22 ,microcalcification ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,atherosclerosis ,business ,Biomarkers ,Calcification - Abstract
Background: this study aims to investigate the possible association among the histopathologic features of carotid plaque instability, the presence of micro- or macrocalcifications, the expression of in situ inflammatory biomarkers, and the occurrence of the major risk factors in this process in a large series of carotid plaques. Methods: a total of 687 carotid plaques from symptomatic and asymptomatic patients were collected. Histological evaluation was performed to classify the calcium deposits in micro or macrocalcifications according to their morphological features (location and size). Immunohistochemistry was performed to study the expression of the main inflammatory biomarkers. Results: results here reported demonstrated that calcifications are very frequent in carotid plaques, with a significant difference between the presence of micro- and macrocalcifications. Specifically, microcalcifications were significantly associated to high inflamed unstable plaques. Paradoxically, macrocalcifications seem to stabilize the plaque and are associated to a M2 macrophage polarization instead. Discussion: the characterization of mechanisms involved in the formation of carotid calcifications can lay the foundation for developing new strategies for the management of patients affected by carotid atherosclerosis. Data of this study could provide key elements for an exhaustive evaluation of carotid plaque calcifications allowing to establish the risk of associated clinical events.
- Published
- 2021
31. Ultrasound-Guided Laser Ablation After Excisional Vacuum-Assisted Breast Biopsy for Small Malignant Breast Lesions: Preliminary Results
- Author
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Gianluca Vanni, Donatella Ferrari, Vincenzo De Stasio, Rosaria Meucci, Guglielmo Manenti, Lucia Anemona, Feliciana Lamacchia, Chiara Adriana Pistolese, Oreste Claudio Buonomo, Carla Di Stefano, and Tommaso Perretta
- Subjects
Breast biopsy ,Cancer Research ,medicine.medical_specialty ,Neoplasm, Residual ,Vacuum ,Biopsy ,medicine.medical_treatment ,Breast Neoplasms ,lcsh:RC254-282 ,ablation ,030218 nuclear medicine & medical imaging ,law.invention ,03 medical and health sciences ,Mini invasive surgery ,0302 clinical medicine ,Settore MED/36 ,law ,Humans ,Minimally Invasive Surgical Procedures ,Medicine ,Breast ,Ultrasonography, Interventional ,Aged ,mini-invasive ,Laser ablation ,medicine.diagnostic_test ,business.industry ,Carcinoma, Ductal, Breast ,Ultrasound ,Middle Aged ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Ablation ,Laser ,excisional biopsy ,Ultrasound guided ,Tumor Burden ,laser ,Oncology ,030220 oncology & carcinogenesis ,Vacuum-assisted breast biopsy ,Feasibility Studies ,Female ,Original Article ,Laser Therapy ,Radiology ,business ,Mammography - Abstract
Background: The purpose of this preliminary study is to evaluate the feasibility of the excisional ultrasound (US) guided vacuum-assisted breast biopsy (VAE), followed by US-guided Laser Interstitial Thermal Therapy (LITT) in the treatment of unifocal ductal breast carcinomas ≤ 1 cm and estimate the ablation rate analyzing the final histopathological results after subsequent surgical excision. Methods: In a single session 11 female patients with unifocal less than a centimeter breast cancer underwent 2 different minimally invasive percutaneous US-guided techniques: a VAE breast biopsy with an 8 G needle to remove the lesion and, immediately after, a LITT ablation in the biopsy site. Four weeks later, all patients underwent radiological follow-up. Afterward, a systematic surgery was performed, the ablation rate was calculated, and iconographic and histological features were correlated. Results: Average maximum diameter of the lesions was 7.6 mm (5-10 mm). No patient reported pain or discomfort during procedure. 1/11 patient (9.1%) reported an early minor complication (a small superficial skin burn). After surgical excision, the histopathological evaluation reported in 10/11 cases (90.9%) complete ablation of the target lesion. In only one case (9.1%) residual cancer was detected. The necrotic-hemorrhagic cavities showed a mean maximum diameter of 27.3 mm (20-35 mm). Conclusions: Laser ablation performed after excisional biopsy could be considered a valid alternative to surgical excision for the treatment of lesions ≤ 1 cm, if carried out by expert radiologists. The association of these minimally invasive percutaneous methods has proven to be reliable, fast, and safe with an ablation rate of 90.9% and excellent aesthetic results. RM and CESM are potentially able to quantifying treatment results and to follow-up the ablation effects.
- Published
- 2021
32. Evaluation of the Usefulness of FDG-PET/CT for Nodal Staging of Breast Cancer
- Author
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Ilaria Portarena, Emanuele Caredda, Paola Valeria Marchese, Oreste Claudio Buonomo, Leonardo Palombi, Lucia Anemona, Paolo Orsaria, Orazio Schillaci, Giuseppe Petrella, Agostino Chiaravalloti, and Brisida Titka
- Subjects
Adult ,Male ,Cancer Research ,medicine.medical_specialty ,nodal staging ,Breast Neoplasms ,Sensitivity and Specificity ,030218 nuclear medicine & medical imaging ,Metastasis ,Breast Neoplasms, Male ,03 medical and health sciences ,Basal (phylogenetics) ,0302 clinical medicine ,Breast cancer ,Settore MED/36 - Diagnostica per Immagini e Radioterapia ,Fluorodeoxyglucose F18 ,Predictive Value of Tests ,Positron Emission Tomography Computed Tomography ,Progesterone receptor ,medicine ,80 and over ,metabolic pathways ,Humans ,Lymph node ,Mastectomy ,prognostic microenvironment ,Aged ,Neoplasm Staging ,FDG-PET/CT ,Aged, 80 and over ,Axilla ,Female ,Lymph Nodes ,Lymphatic Metastasis ,Middle Aged ,medicine.diagnostic_test ,business.industry ,General Medicine ,medicine.disease ,medicine.anatomical_structure ,Oncology ,Positron emission tomography ,030220 oncology & carcinogenesis ,Immunohistochemistry ,Histopathology ,Nuclear medicine ,business - Abstract
BACKGROUND/AIM: Positron emission tomography/computed tomography (PET/CT) with 18F-fluorodeoxyglucose (18F-FDG) has recently been used to investigate lymph node (LN) metastases and several predictive features in patients with breast cancer (BC). The aim of this study was to assess the value of this non-invasive imaging procedure for axillary staging. PATIENTS AND METHODS: Fifty patients with early primary unilateral, locally advanced, or recurrent invasive operable BC were enrolled. All patients underwent preoperative 18F-FDG PET/CT, and the results were compared with the histopathology of dissected axillary LNs and their biological and immunohistochemical characteristics. The diagnostic performance of 18F-FDG PET/CT in detecting LN metastases from primary or recurrent BC was analyzed. The mean values of the initial PET/CT parameters, including the primary tumour (SUV T) and ipsilateral axillary LNs (SUV LN), were compared with the clinicopathological features of patients to determine their usefulness for predicting clinical interactions. RESULTS: The sensitivity, specificity, overall accuracy, positive predictive value, and negative predictive value of 18F-FDG PET/CT for axillary LN staging were 87%, 90%, 88%, 93%, and 82%, respectively. Bivariate analyses showed strong interactions of nuclear grade (p=0.05), progesterone receptor expression (p=0.001), Ki-67 index (0.027), and local relapse with the SUV T. A high SUV LN value was significantly correlated with a higher nuclear grade score (p=0.05), oestrogen receptor negativity (p=0.001), progesterone receptor negativity (p=0.014), a high Ki-67 index (>20%; p=0.048), LN metastasis (p
- Published
- 2018
33. Recurrent Sweet's syndrome in a patient with multiple myeloma
- Author
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Maria Cantonetti, Ida Provenzano, Francesca Passarelli, Livio Pupo, Daniela Nasso, Carmelo Gurnari, Sara Vaccarini, Luca Franceschini, Lucia Anemona, and Manuela Rizzo
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Oncology ,medicine.medical_specialty ,Sweet's syndrome ,Case Report ,Case Reports ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,stomatognathic system ,immune system diseases ,Internal medicine ,hemic and lymphatic diseases ,medicine ,multiple myeloma ,Multiple myeloma ,business.industry ,digestive, oral, and skin physiology ,food and beverages ,General Medicine ,medicine.disease ,Settore MED/15 ,030220 oncology & carcinogenesis ,business ,DISEASE RELAPSE - Abstract
Key Clinical Message We report on a case of Sweet's syndrome associated with multiple myeloma, as harbinger for disease relapse.
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- 2018
34. The role of obesity in carotid plaque instability: Interaction with age, gender, and cardiovascular risk factors
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Manuela Montanaro, Massimo Federici, Manuel Scimeca, Arnaldo Ippoliti, Lucia Anemona, Marina Cardellini, Elena Bonanno, Alessandro Mauriello, Iacopo Maria Legramante, Valentina Rovella, Nicola Di Daniele, Andrea Saggini, and Giuseppe Santeusanio
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Male ,lcsh:Diseases of the circulatory (Cardiovascular) system ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Rome ,Comorbidity ,030204 cardiovascular system & hematology ,Body Mass Index ,0302 clinical medicine ,Risk Factors ,Medicine ,Carotid Stenosis ,Stroke ,Original Investigation ,Endarterectomy ,Plaque ,Atherosclerotic ,Age Factors ,Middle Aged ,Prognosis ,Metabolic syndrome ,Plaque, Atherosclerotic ,Carotid Arteries ,Female ,medicine.symptom ,Cardiology and Cardiovascular Medicine ,Obesity paradox ,medicine.medical_specialty ,Histology ,Settore MED/08 - Anatomia Patologica ,Asymptomatic ,Risk Assessment ,03 medical and health sciences ,Age ,Sex Factors ,Internal medicine ,Humans ,Obesity ,Risk factor ,Carotid ,Aged ,Rupture ,Rupture, Spontaneous ,business.industry ,Spontaneous ,Gender ,Odds ratio ,medicine.disease ,Cerebrovascular Disorders ,lcsh:RC666-701 ,business ,Body mass index ,030217 neurology & neurosurgery - Abstract
Background In the last decade, several studies have reported an unexpected and seemingly paradoxical inverse correlation between BMI and incidence of cardiovascular diseases. This so called “obesity paradox effect” has been mainly investigated through imaging methods instead of histologic evaluation, which is still the best method to study the instability of carotid plaque. Therefore, the purpose of our study was to evaluate by histology the role of obesity in destabilization of carotid plaques and the interaction with age, gender and other major cerebrovascular risk factors. Methods A total of 390 carotid plaques from symptomatic and asymptomatic patients submitted to endarterectomy, for whom complete clinical and laboratory assessment of major cardiovascular risk factors was available, were studied by histology. Patients with a BMI ≥ 30.0 kg/m2 were considered as obese. Data were analyzed by multivariate logistic regression and for each variable in the equation the estimated odds ratio (OR) was calculated. Results Unstable carotid plaque OR for obese patients with age
- Published
- 2018
35. Dysplasia of Granulocytes in a Patient with HPV Disease, Recurrent Infections, and B Lymphopenia: A Novel Variant of WHIM Syndrome?
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Viviana Moschese, Giusella M. F. Moscato, Erica Giacobbi, Lucia Anemona, Silvia Di Cesare, Gigliola Di Matteo, Massimo Andreoni, and Alessandro Mauriello
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0301 basic medicine ,Hypersegmented neutrophil ,Pathology ,medicine.medical_specialty ,Settore MED/17 - Malattie Infettive ,WHIM ,Settore MED/08 ,Case Report ,Gene mutation ,B lymphopenia ,CXCR4 ,Pediatrics ,HPV disease ,dysplasia of granulocytes ,myelokathexis ,03 medical and health sciences ,medicine ,Immunodeficiency ,Myelokathexis ,business.industry ,lcsh:RJ1-570 ,lcsh:Pediatrics ,medicine.disease ,030104 developmental biology ,Dysplasia ,Immunology ,Pediatrics, Perinatology and Child Health ,Myelopoiesis ,business ,WHIM syndrome - Abstract
WHIM syndrome is a condition for which affected persons have chronic peripheral neutropenia, lymphopenia, abnormal susceptibility to human papilloma virus infection and myelokathexis. Myelokathexis refers to the retention of mature neutrophils in the bone marrow which accounts for degenerative changes and hypersegmentation. Most patients present heterozygous autosomal dominant mutations of the gene encoding CXCR4. Consequently, aberrant CXCL12/CXCR4 signalling impairs the receptor down-regulation causing hyperactivation (gain of function) that affects bone marrow homing for myelopoiesis and lymphopoiesis and the release of neutrophils in the bloodstream. We report the case of a 26-yr-old female with severe foot and hand cutaneous warts since childhood, recalcitrant genital condylomatas, bacterial infections, and intraepithelial cervical neoplasia. Laboratory tests revealed severe B lymphopenia and HPV high and low risk types. HIV testing was negative. Not only CXCR4, but also GATA2, NEMO and CD40L gene mutations were excluded. BM smears revealed, in the presence of a normal cellularity, hyperplasia of myeloid cells (MPO positive) and karyorrhexis, especially in neutrophils and eosinophils. Of note, neutrophils with altered lobation of nuclei connected by long thin chromatin filaments were observed. Our patient presented a clinical and histological picture reminiscent of WHIM in the presence of normal peripheral neutrophil counts and wild-type CXCR4 gene. Although the BM did not reveal a classical pattern of myelokathexis, the observation of consistent signs of neutrophil dysplasia has fuelled the hypothesis of a novel WHIM variant or a novel immunodeficiency. We speculate that abnormalities that affect CXCR4/CXCL12 pair, including GRK levels or activity, might be responsible for this WHIM-like disorder.
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- 2017
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36. Hypertension in kidney transplantation is associated with an early renal nerve sprouting
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Lucia Anemona, Manfredi Tesauro, Elena Giannini, Nicola Di Daniele, Filippo Borri, Pierluigi Bove, D’Urso Gabriele, Andrea Saggini, Alessandro Anselmo, Gerry Melino, Alessandro Mauriello, Valentina Rovella, Erica Giacobbi, Giampiero Palmieri, and Guardini Valentina
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Male ,medicine.medical_specialty ,hypertension ,Sympathetic Nervous System ,030232 urology & nephrology ,kidney transplantation ,sympathetic renal innervation ,Settore MED/08 ,Blood Pressure ,Settore MED/09 ,030204 cardiovascular system & hematology ,Artificial kidney ,Kidney ,Kidney Failure ,03 medical and health sciences ,0302 clinical medicine ,Renal Artery ,medicine.artery ,Internal medicine ,Medicine ,Humans ,Renal artery ,Chronic ,Renal stem cell ,Kidney transplantation ,Aged ,Denervation ,Transplantation ,business.industry ,Original Articles ,medicine.disease ,Nerve Regeneration ,medicine.anatomical_structure ,Blood pressure ,Settore MED/24 ,Nephrology ,Cardiology ,Kidney Failure, Chronic ,Female ,nerve sprouting ,Hypertension ,Kidney Transplantation ,business - Abstract
Background. Normalization of arterial pressure occurs in just a few patients with hypertensive chronic kidney disease undergoing kidney transplantation. Hypertension in kidney transplant recipients may be related to multiple factors. We aimed to assess whether hypertension in kidney-transplanted patients may be linked to reinnervation of renal arteries of the transplanted kidney. Methods. We investigated renal arteries innervation from native and transplanted kidneys in three patients 5 months, 2 years and 11 years after transplantation, respectively. Four transplanted kidneys from non-hypertensive patients on immunosuppressive treatment without evidence of hypertensive arteriolar damage were used as controls. Results . Evidence of nerve sprouting was observed as early as 5 months following transplantation, probably originated from ganglions of recipient patient located near the arterial anastomosis and was associated with mild hypertensive arteriolar damage. Regeneration of periadventitial nerves was already complete 2 years after transplantation. Nerve density tended to reach values observed in native kidney arteries and was associated with hypertension-related arteriolar lesions in transplanted kidneys. Control kidneys, albeit on an immunosuppressive regimen, presented only a modest regeneration of sympathetic nerves. Conclusions . Our results suggest that the considerable increase in sympathetic nerves, as found in patients with severe arterial damage, may be correlated to hypertension rather than to immunosuppressive therapy, thus providing a morphological basis for hypertension recurrence despite renal denervation.
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- 2017
37. Programmed death ligand 1 expression in prostate cancer cells is associated with deep changes of the tumor inflammatory infiltrate composition
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Rita Bonfiglio, Elena Bonanno, Nicoletta Urbano, Manuel Scimeca, Sara Fazi, Lucia Anemona, Orazio Schillaci, Chiara Cerroni, Manuela Montanaro, and Alessandro Mauriello
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PD-L1 ,Male ,Settore MED/06 - Oncologia Medica ,Urology ,Population ,030232 urology & nephrology ,Settore MED/08 ,CD38 ,cancer immune-escape ,M2 polarization ,macrophages ,prostate cancer ,PTX3 ,B7-H1 Antigen ,Cancer immune-escape ,Macrophages ,Prostate cancer ,03 medical and health sciences ,0302 clinical medicine ,Settore MED/36 - Diagnostica per Immagini e Radioterapia ,TIGIT ,medicine ,Humans ,education ,Retrospective Studies ,Inflammation ,CD20 ,education.field_of_study ,biology ,CD68 ,business.industry ,Prostatic Neoplasms ,Middle Aged ,medicine.disease ,Serum Amyloid P-Component ,C-Reactive Protein ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,Immunohistochemistry ,business - Abstract
Background The main aim of this study was to investigate the putative correlation between the composition of intratumoral inflammatory infiltrate and the expression of programmed death ligand 1 (PD-L1) by prostate cancer cells. In addition, we evaluated the correlation between the expression of PD-L1 and PTX3. Methods We enrolled 100 patients from which we collected one surgical sample each. Paraffin serial sections were obtained to perform histological classifications and tissues microarray construction. Serial tissues microarray paraffin sections were also used for PD-L1 analysis and intratumoral inflammatory infiltrate characterization (CD4, CD8, CD57, CD3, PD1, PSGL-1, TIGIT, CD20, CD38, CD68, CD163, and PTX3) by immunohistochemistry . Results Our result showed a significant increase of the number of both PD-L1 and PTX3 positive cells in prostate tumors respect to benign lesions. Inflammatory infiltrate of PD-L1 positive prostate cancer lesions was characterized by a decrease of both PD1 positive lymphocytes and tumor-infiltrated macrophages, mainly M2 subpopulation. Also, PTX3 expression showed an inverse correlation with the number of PD-L1 positive prostate cancer cells. Conclusions If confirmed, our data could be useful to predict the variations of the inflammatory population related to PD-L1 expression in prostate cancer. This can lay the foundation to establish therapeutic protocols able to inhibit the PD-L1 activity and, at the same time, to reactivate the antitumor inflammatory process.
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- 2019
38. Elemental analysis of histological specimens: a method to unmask nano asbestos fibers
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Augusto Orlandi, Luigi Tonino Marsella, Manuel Scimeca, Elena Bonanno, Antonio Pietroiusti, Filippo Milano, and Lucia Anemona
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Male ,0301 basic medicine ,Pathology ,medicine.medical_specialty ,Lung Neoplasms ,Histology ,occupational exposure ,Nanofibers ,Biophysics ,Settore MED/08 - Anatomia Patologica ,Asbestos fibers ,medicine.disease_cause ,Asbestos ,03 medical and health sciences ,0302 clinical medicine ,Through transmission ,Technical Note ,medicine ,Humans ,Lung cancer ,lcsh:QH301-705.5 ,Close contact ,business.industry ,Cell Biology ,medicine.disease ,3. Good health ,lung cancer ,030104 developmental biology ,lcsh:Biology (General) ,EDX microanalysis ,030220 oncology & carcinogenesis ,Female ,Transmission Electron Microscopy ,Occupational exposure ,business - Abstract
There is recent mounting evidence that nanoparticles may have enhanced toxicological potential in comparison to the same material in the bulk form. The aim of this study was to develop a new method for unmask asbestos nanofibers from Formalin-Fixed, Paraffin-Embedded tissue. There is an increasing amount of evidence that nanoparticles may enhance toxicological potential in comparison to the same material in the bulk form. The aim of this study was to develop a new method to unmask asbestos nanofibers from Formalin-Fixed Paraffin-Embedded (FFPE) tissue. For the first time, in this study we applied Energy Dispersive X-ray (EDX) microanalysis through transmission electron microscopy to demonstrate the presence of asbestos nanofibers in histological specimens of patients with possible occupational exposure to asbestos. The diagnostic protocol was applied to 10 randomly selected lung cancer patients with no history of previous asbestos exposure. We detected asbestos nanofibers in close contact with lung cancer cells in two lung cancer patients with previous possible occupational exposure to asbestos. We were also able to identify the specific asbestos iso-type, which in one of the cases was the same rare variety used in the workplace of the affected patient. By contrast, asbestos nanofibers were not detected in lung cancer patients with no history of occupational asbestos exposure. The proposed technique can represent a potential useful tool for linking the disease to previous workplace exposure in uncertain cases. Furthermore, Formalin-Fixed Paraffin-Embedded (FFPE) tissues stored in the pathology departments might be re-evaluated for possible etiological attribution to asbestos in the case of plausible exposure. Since diseases acquired through occupational exposure to asbestos are generally covered by workers’ insurance in most countries, the application of the protocol used in this study may have also relevant social and economic implications.Â
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- 2016
39. A case of oral mycosis fungoides successfully treated by combination of alemtuzumab and chemotherapy
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Francesco Buccisano, Luca Franceschini, E. Bruno, Massimiliano Postorino, Daniela Nasso, Ida Provenzano, I. Onnis, M. I. Del Principe, S. Mauramati, S. Amadori, Federico Meconi, Manuela Rizzo, Lucia Anemona, Sara Vaccarini, Livio Pupo, Annagiulia Zizzari, and Maria Cantonetti
- Subjects
Mouth neoplasm ,Oncology ,medicine.medical_specialty ,Chemotherapy ,Mycosis fungoides ,Pathology ,Vincristine ,Cyclophosphamide ,business.industry ,medicine.medical_treatment ,Hematology ,General Medicine ,medicine.disease ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Internal medicine ,Prednisolone ,Medicine ,Alemtuzumab ,business ,Settore MED/15 - Malattie del Sangue ,Etoposide ,medicine.drug - Published
- 2016
40. HIV-associated primary cutaneous anaplastic large cell lymphoma: a clinicopathological subset with more aggressive behavior? Case report and review of the literature
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Luca Bianchi, Andrea Saggini, Alessandro Di Stefani, Sergio Chimenti, Claudia Torti, Lucia Anemona, and Loredana Sarmati
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education.field_of_study ,Pathology ,medicine.medical_specialty ,Histology ,business.industry ,Incidence (epidemiology) ,medicine.medical_treatment ,Population ,Primary cutaneous anaplastic large cell lymphoma ,Immunosuppression ,Dermatology ,medicine.disease ,Cutaneous lymphoma ,Pathology and Forensic Medicine ,Acquired immunodeficiency syndrome (AIDS) ,hemic and lymphatic diseases ,medicine ,Immunohistochemistry ,education ,business ,Anaplastic large-cell lymphoma - Abstract
Human immunodeficiency virus (HIV)-infected patients carry an increased risk of lymphomagenesis. Although the majority of HIV-related lymphomas have a B-cell phenotype, the incidence of peripheral T-cell lymphomas (PTCL), including primary cutaneous subtypes, may be up to 15-fold higher than in the general population, with anaplastic large cell lymphomas (ALCL) accounting for 18-28% of HIV-associated PTCL. In contrast to systemic ALCL, the relation between HIV infection and primary cutaneous ALCL has been relatively neglected in the literature. We report the case of a primary cutaneous ALCL occurring in a 76-year-old patient with advanced HIV infection, and showing unusually aggressive course. Neither ALK1 immunohistochemical positivity nor evidence of EBV infection were detected; staging procedures at initial presentation ruled out systemic involvement. We provide a summary of the literature regarding primary cutaneous ALCL in HIV-infected patients. We draw attention to clinicopathological features, prognostic implications and therapeutic quandaries of HIV-related primary cutaneous ALCL. Further, we propose that a significant fraction of HIV-associated cases might represent a more aggressive subset of primary cutaneous ALCL.
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- 2012
41. Increased Sympathetic Renal Innervation in Hemodialysis Patients Is the Anatomical Substrate of Sympathetic Hyperactivity in End-Stage Renal Disease
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Valentina Rovella, Alessandro Anselmo, Pierluigi Bove, Francesca Servadei, Nicola Di Daniele, Gerry Melino, Alessandro Mauriello, Lucia Anemona, and Elena Giannini
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Male ,lcsh:Diseases of the circulatory (Cardiovascular) system ,medicine.medical_specialty ,medicine.medical_treatment ,sympathetic renal innervation ,Settore MED/08 ,Autopsy ,Kidney ,End stage renal disease ,Settore MED/24 - Urologia ,histology ,Renal Dialysis ,Adventitia ,Internal medicine ,medicine ,Humans ,Sympathectomy ,end‐stage renal disease ,Dialysis ,Original Research ,Aged ,Denervation ,hemodialysis ,Kidney in Cardiovascular Disease ,business.industry ,Middle Aged ,Surgery ,medicine.anatomical_structure ,lcsh:RC666-701 ,Case-Control Studies ,Hypertension ,Cardiology ,Kidney Failure, Chronic ,Female ,Hemodialysis ,Adrenergic Fibers ,Cardiology and Cardiovascular Medicine ,business ,Catheter Ablation and Implantable Cardioverter-Defibrillator - Abstract
Background Renal denervation represents an emerging treatment for resistant hypertension in patients with end‐stage renal disease, but data about the anatomic substrate of this treatment are lacking. Therefore, the aim of this study was to investigate the morphological basis of sympathetic hyperactivity in the setting of hemodialysis patients to identify an anatomical substrate that could warrant the use of this new therapeutic approach. Methods and Results The distribution of sympathetic nerves was evaluated in the adventitia of 38 renal arteries that were collected at autopsy or during surgery from 25 patients: 9 with end‐stage renal disease on dialysis ( DIAL group) and 16 age‐matched control nondialysis patients ( CTRL group). Patients in the DIAL group showed a significant increase in nerve density in the internal area of the peri‐adventitial tissue (within the first 0.5 mm of the beginning of the adventitia) compared with the CTRL group (4.01±0.30 versus 2.87±0.28×mm 2 , P =0.01). Regardless of dialysis, hypertensive patients with signs of severe arteriolar damage had a greater number of nerve endings in the most internal adventitia, and this number was significantly higher than in patients without hypertensive arteriolar damage (3.90±0.36 versus 2.87±0.41×mm 2 , P =0.04), showing a correlation with hypertensive arteriolar damage rather than with hypertensive clinical history. Conclusions The findings from this study provide a morphological basis underlying sympathetic hyperactivity in patients with end‐stage renal disease and might offer useful information to improve the use of renal denervation in this group of patients.
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- 2015
42. Diffuse and Active Inflammation Occurs in Both Vulnerable and Stable Plaques of the Entire Coronary Tree
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Robert S. Schwartz, Alessandro Mauriello, Giampiero Palmieri, Stefano Fratoni, Lucia Anemona, Luigi Giusto Spagnoli, Elena Bonanno, and Giuseppe Sangiorgi
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medicine.medical_specialty ,business.industry ,Inflammation ,Anatomical pathology ,medicine.disease ,Culprit ,medicine.anatomical_structure ,Internal medicine ,Circulatory system ,medicine ,Cardiology ,Myocardial infarction ,medicine.symptom ,Active inflammation ,business ,Cardiology and Cardiovascular Medicine ,Cause of death ,Artery - Abstract
Objectives This study was undertaken to define and compare geographic coronary artery inflammation in patients who were dying of acute myocardial infarction (AMI), chronic stable angina (SA), and noncardiac causes (CTRL). Background Biochemical markers and flow cytometry provide indirect evidence of diffuse coronary inflammation in patients dying of acute coronary syndromes. Yet no histopathologic studies have corroborated these findings. A key unanswered question is whether the inflammatory burden involves the entire coronary tree or is limited to a few plaques. Methods We examined 544 coronary artery segments from 16 patients with AMI, 109 segments from 5 patients with SA, and 304 coronary segments from 9 patients with CTRL. Results An average of 6.8 ± 0.5 vulnerable segments per patient were found in the AMI group (in addition to culprit lesions) compared with an average of 0.8 ± 0.3 and 1.4 ± 0.3 vulnerable lesions/patient in the SA and CTRL groups, respectively. The AMI group, independent of the type of plaque observed, showed significantly more inflammatory infiltrates compared with the SA and CTRL groups (121.6 ± 12.4 cell × mm2vs. 37.3 ± 11.9 cell × mm2vs. 26.6 ± 6.8 cell × mm2, p = 0.0001). In AMI patients, active inflammation was not only evident within the culprit lesion and vulnerable plaques but also involved stable plaques. These showed a three- to four-fold higher inflammation than vulnerable and stable plaques from the SA and CTRL groups, respectively. Conclusions This histopathologic study found that both vulnerable and stable coronary plaques of patients dying of AMI are diffusely infiltrated by inflammatory cells.
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- 2005
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43. Myopericytoma of the liver hilus: a case report
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Lucia Anemona, Giuseppe Tisone, Amedeo Ferlosio, Tommaso Maria Manzia, and Augusto Orlandi
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Male ,Pathology ,medicine.medical_specialty ,business.industry ,Myopericytoma ,Liver Neoplasms ,Soft Tissue Neoplasms ,Soft tissue pathology ,Biological potential ,Settore MED/08 - Anatomia Patologica ,Middle Aged ,medicine.disease ,Immunohistochemistry ,Middle age ,Patient care ,Pathology and Forensic Medicine ,medicine.anatomical_structure ,Smooth muscle ,Biomarkers, Tumor ,Medicine ,Humans ,Differential diagnosis ,business ,Pericytes ,Subcutaneous tissue - Abstract
Pericytic tumours is a new category recently introduced in soft tissue pathology describing those entities sharing evidence of myoid differentiation and a tendency of spindle or more rounded cells to grow in a perivascular fashion. The great majority of pericytic tumours are benign but recurrence has been described as well as very rare malignant cases with an aggressive behaviour. Although pericytic tumours arise most commonly in the subcutaneous tissue of distal extremities in middle age patients, additional cases have been recently described in many other organs. Here we describe a case of a 57-year-old man with an 8.5 cm mass of the liver hilus diagnosed as myopericytoma, with particular reference to its biological potential and differential diagnosis. It is important for the pathologists to take this diagnosis into consideration as well for a better management of patient care.
- Published
- 2014
44. CSF biomarkers in superficial siderosis: a new tool for diagnosis and evaluation of therapeutic efficacy of deferiprone--a case report
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Giuseppe Sancesario, Giulia Maria Sancesario, Tommaso Schirinzi, Antonio Pisani, and Lucia Anemona
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medicine.medical_specialty ,Neurology ,business.industry ,Dermatology ,General Medicine ,medicine.disease ,Superficial siderosis ,Surgery ,Psychiatry and Mental health ,chemistry.chemical_compound ,chemistry ,Csf biomarkers ,medicine ,Settore MED/26 - Neurologia ,Neurology (clinical) ,Neurosurgery ,Intensive care medicine ,business ,Deferiprone ,Neuroradiology - Published
- 2014
45. Rituximab single agent in age-related Epstein-Barr virus associated B cell disorder complicated by autoimmune anemia and pure red cell aplasia
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Mariagiovanna Cefalo, Luigi Di Caprio, Lucia Anemona, Giovanna De Santis, Massimiliano Postorino, Giovanni Del Poeta, Sergio Amadori, Adriano Venditti, Francesco Buccisano, Maria Ilaria Del Principe, Chiara Sarlo, Luca Maurillo, and Erica Giacobbi
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Male ,Murine-Derived ,Aging ,Epstein-Barr Virus Infections ,Red-Cell Aplasia, Pure ,Humans ,Middle Aged ,Lymphoproliferative Disorders ,B-Lymphocytes ,Anemia, Hemolytic, Autoimmune ,Antibodies, Monoclonal, Murine-Derived ,Anemia ,Pure red cell aplasia ,medicine.disease_cause ,Antibodies ,Monoclonal ,medicine ,Pure ,B cell ,biology ,business.industry ,Hematology ,General Medicine ,medicine.disease ,Virology ,Epstein–Barr virus ,medicine.anatomical_structure ,Immunology ,biology.protein ,Rituximab ,Red-Cell Aplasia ,Autoimmune hemolytic anemia ,Antibody ,business ,Hemolytic ,Settore MED/15 - Malattie del Sangue ,medicine.drug ,Autoimmune - Published
- 2014
46. Coronary calcification identifies the vulnerable patient rather than the vulnerable Plaque
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Elena Bonanno, Erica Giacobbi, Alessandro Mauriello, Francesca Servadei, Giuseppe Biondi Zoccai, Sara Casella, and Lucia Anemona
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Male ,Vasculitis ,medicine.medical_specialty ,Multivariate analysis ,Population ,Myocardial Infarction ,Lumen (anatomy) ,Coronary Artery Disease ,Settore MED/08 - Anatomia Patologica ,medicine.disease_cause ,Risk Assessment ,coronary calcification ,histology ,plaque vulnerability ,vulnerable patient ,Risk Factors ,Internal medicine ,medicine ,80 and over ,Humans ,Inverse correlation ,education ,Vascular Calcification ,Plaque ,Atherosclerotic ,Aged ,Aged, 80 and over ,education.field_of_study ,business.industry ,Coronary Thrombosis ,Middle Aged ,medicine.disease ,Vulnerable plaque ,Coronary Vessels ,Female ,Multivariate Analysis ,Plaque, Atherosclerotic ,Coronary artery calcification ,Cardiology ,Cardiology and Cardiovascular Medicine ,business ,Cardiovascular outcomes ,Calcification - Abstract
Objective Presence of coronary artery calcium (CAC) is associated with a high risk of adverse cardiovascular outcomes. Nevertheless, although CAC is a marker of atherosclerosis it is still uncertain whether CAC is a marker of plaque vulnerability. Therefore, the aim of this study was to verify if calcification identifies a vulnerable patient rather than the vulnerable plaque. Methods A morphologic and morphometric study on 960 coronary segments (CS) of 2 groups of patients was performed: (i) 17 patients who died from AMI (510 CS); (ii) 15 age-matched control patients without cardiac history (CTRL, 450 CS). Results Calcification was found in 47% CS of AMI and in 24.5% CS of CTRL. The area of calcification was significantly higher in AMI compared to CTRL ( p = 0.001). An inverse correlation was found between the extension of calcification and cap inflammation ( r 2 = 0.017; p = 0.003). Multivariate regression analysis demonstrated that the calcification was not correlated with the presence of unstable plaques ( p = 0.65). Similarly, the distance of calcification from the lumen did not represent an instability factor ( p = 0.68). Conclusion The present study suggests that CAC score evaluation represents a valid method to define the generic risk of acute coronary events in a population, but it is not useful to identify the vulnerable plaque that need to be treated in order to prevent an acute event.
- Published
- 2012
47. Asymptomatic carotid plaque rupture with unexpected thrombosis over a non-canonical vulnerable lesion
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Lucia Anemona, Alessandro Mauriello, Francesca Servadei, Doriana Liotti, Erica Giacobbi, Luigi Giusto Spagnoli, and Giuseppe Sangiorgi
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Male ,medicine.medical_specialty ,Pathology ,carotid plaque ,Settore MED/08 - Anatomia Patologica ,medicine.disease_cause ,Asymptomatic ,Lesion ,Necrosis ,Ischemia ,Risk Factors ,Internal medicine ,morphology ,medicine ,Humans ,Stroke ,carotid plaque, morphology ,Aged ,Inflammation ,Metabolic Syndrome ,Endarterectomy, Carotid ,Framingham Risk Score ,business.industry ,Thrombosis ,Middle Aged ,medicine.disease ,Atherosclerosis ,Vulnerable plaque ,Lipids ,Plaque, Atherosclerotic ,Carotid Arteries ,Acute Disease ,Cardiology ,Disease Progression ,Female ,medicine.symptom ,Metabolic syndrome ,Cardiology and Cardiovascular Medicine ,business ,Calcification - Abstract
Objective Several studies have demonstrated that carotid plaque rupture and thrombosis represent the most important factors correlated with the onset of acute cerebrovascular symptoms. Nevertheless, ruptured thrombotic plaques have been described also in asymptomatic patients. What still needs to be clarified is why a plaque rupture leads either to an acute ischemic syndrome or, in a minor group of patients, remains asymptomatic. The purpose of this study was to systematically compare the histologic features of thrombotic plaques both in asymptomatic and symptomatic patients in order to identify specific findings that could explain the peculiar clinical behavior that characterizes each of the clinical settings. Methods A total of 157 thrombotic plaques from 60 asymptomatic patients and 97 with major stroke who consecutively underwent CEA were serially sectioned and studied by histology. Results A minute cap disruption very frequently characterizes thrombotic plaques of asymptomatic patients and it was always smaller than large ulcers observed in thrombotic symptomatic plaques (651±687μm vs. 4150±3526, p =0.001). In asymptomatics this typical feature was associated with fewer inflammatory cells (20.1±8.8 vs. 33.9±26.1cells×hpf, p =0.001), smaller lipidic–necrotic core (33.9%±2.9% vs. 42.0%±2.4%; p =0.04) and larger calcification (16.2±12.8% vs. 8.1±12.2%, p =0.02). Symptomatic patients with thrombotic plaques showed higher incidence of metabolic syndrome ( p =0.002) and moderate-high Framingham risk scores ( p =0.001) comparing to asymptomatic individuals. Conclusion The transformation from a stable to a vulnerable plaque is a gradual process in the natural history of the disease and plaque rupture is an event not necessarily occurring at a late phase but also at earlier one. In this case, the rupture will be most likely smaller and clinically asymptomatic.
- Published
- 2011
48. Pegylated Liposomal Doxorubicin, Bleomycin, Vinblastine and Dacarbazine (CBVD) in the treatment of advanced primary cutaneous lymphomas
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Maria Cantonetti, Giuseppe Alfonso Lombardo, Manuela Rizzo, Sara Vaccarini, Malgorzata Monika Trawinska, Livio Pupo, Elisabetta Abruzzese, Lucia Anemona, Massimiliano Postorino, Roberta Cannarsa, Micol Quaresima, Alessandra Spagnoli, Tullio Faraggiana, and Daniela Renzi
- Subjects
medicine.medical_specialty ,Mycosis fungoides ,business.industry ,Dacarbazine ,Immunology ,macromolecular substances ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Gastroenterology ,Lymphoma ,Vinblastine ,Transplantation ,Median follow-up ,Internal medicine ,medicine ,Rituximab ,business ,Progressive disease ,medicine.drug - Abstract
Abstract 3717 Poster Board III-653 Introduction Primary cutaneous lymphomas (PCL) present in the majority of histologic variants an indolent behaviour and a good prognosis with a prolonged survival. In a very small number of patients (pts), PCL is aggressive at the onset while in pts who are resistant or have relapsed after repeated topic or systemic therapies an advanced disease is more frequently observed. In these patients no therapy is capable of inducing a stable remission of the disease. The efficacy and low toxicity of Pegylated Liposomal Doxorubicin (PLD) as a single agent in second-line therapy of PCL was recently demonstrated. In our study we tested the safety and efficacy of PLD (Caelyx®) (C) in association with three drugs of proven effectiveness in nodal lymphoprolipherative and other primary cutaneous neoplastic disorders: Bleomycin (B), Vinblastine (B) and Dacarbazine (D) (CBVD). Patients and Methods From February 2003 to December 2008 we observed 37 consecutive pts with advanced PCL: 19 Cutaneous T-Cell Lymphomas (CTCL) and 18 cutaneous B-Cell Lymphomas (CBCL). The CTCL pts were: 15 males and 4 females, with median age 59 (27-86) years, of which 7 pts with Anaplastic Large Cell Lymphoma (ALCL) CD30 positive, 7 pts with transformed Mycosis Fungoides (tMF), 3 pts with CD30 negative ALCL, 1pt with Panniculitis-like (Pl) Lymphoma and 1 pt with transformed Sezary Syndrome (tSS), according to the WHO-EORTC consensus classification. Nine pts presented with a nodal involvement and 10 pts were resistant or relapsed after 1 or more systemic treatments. Among the CBCL pts there were: 8 males and 10 females, with median age 61 (42-84) years, 12 pts had a Follicular Centre Lymphoma (FCL), 3 a Marginal Zone Lymphoma (MZL) and 3 a Diffuse Large B-Cell Lymphoma Leg Type (LT). Five pts presented a nodal involvement and 11 pts had received 1 or more previous systemic therapy. All 37 pts received CBVD therapy at following dosage: C: 20 mg/m2, B: 10 mg/m2, V: 6 mg/m2, D: 325 mg/m2 at days 1 and 15, administered intravenously every 4 weeks for a maximum of 6 cycles. Rituximab (R) at dosage of 375 mg/m2 was administered to CBCL pts at 1st day of each cycle. Before the treatment, pts were submitted to a complete staging of disease including TC-scan, bone marrow biopsy, and immunophenotyping of peripheral blood cells. Results In the CTCL group 4 pts received 4 CBVD cycles, 14 pts received 6 CBVD cycles and 1 pt presented a progressive disease after the 1st cycle. Overall Response Rate (ORR) was 94.7% (18/19 pts). Sixteen out of 18 pts (88.8%) had a Complete Remission (CR) with disappearance of nodal involvement and cutaneous lesions, 2/18 pts obtained a Partial Remission (PR) with disappearance of nodal involvement and 75% of cutaneous lesions. In the CBCL group 3 pts received 2 R-CBVD cycles, 3 pts 4 cycles and 11 pts 6 cycles: ORR was 100% and all pts obtained a CR. The occurrence of palmoplantar erytrodisesthesia in 5 pts and grade 2-3 granulocytopenia in 12 pts did not modify the therapy program. Two CTCL pts (1 tSS, 1Pl) and 1 CBCL (LT) pt in CR after therapy received an allogeneic Hemopoietic Stem Cells (HCS) transplant and 1 CBCL(FCL) pt a syngeneic HCS transplant. In CTCL group 11 (61%) pts maintained their response after a median follow up of 15.5 (9-71) months. Among CBCL pts 14 (77.7%) pts are still in CR after a median follow up of 15 (4-36) months. Conclusions Our experience demonstrates that the CBVD association is an effective and safe therapy for advanced PCL in inducing an important tumour burden reduction with a high CR rate. The assessment of response duration requires a longer observation. A larger number of patients in a multicentric trial are needed to confirm our promising results. Disclosures: Off Label Use: liposomial doxorubicin has a peculiar cutaneous tropism and low cardiac toxicity compared with other anthracyclines.
- Published
- 2009
49. Intravenous Administration of Rituximab in the Treatment of Primary Cutaneous B-Cell Lymphomas (PCBCLs): A Retrospective Study
- Author
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Ida Provenzano, Livio Pupo, Massimiliano Postorino, Lucia Anemona, Cecilia Angeloni, Daniela Nasso, Sara Vaccarini, Annagiulia Zizzari, Giuseppe Alfonso Lombardo, Giovangiacinto Paterno, Manuela Rizzo, Maria Cantonetti, Alessandro Mauriello, Enrico Scala, Luca Franceschini, Laura Giannì, and Lorenzo Tonialini
- Subjects
medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,Immunology ,Follicular lymphoma ,Retrospective cohort study ,Cell Biology ,Hematology ,medicine.disease ,Biochemistry ,Gastroenterology ,Lymphoma ,Surgery ,Radiation therapy ,Internal medicine ,Medicine ,Rituximab ,Stage (cooking) ,business ,Adverse effect ,Diffuse large B-cell lymphoma ,medicine.drug - Abstract
Introduction. Rituximab has been demonstrated to be effective either as intralesional or as systemic therapy in PCBCL, We report our experience in the treatment of PCBCL with intravenous Rituximab. P atients and Methods From February 1999 to February 2014 we treated 75 patients: 47 Follicle Center Lymphoma, 23 Marginal Zone Lymphoma, 5 Diffuse Large Lymphoma Leg type. The stage was T1 in 38 pts, T2 in 22 pts and T3 in 15 pts. In 24 patient prior treatment included: CHT (11), Radiotherapy (4), Surgery (4) or alpha2Interferon (IFN) (5). Rituximab at dosage of 375 mg/m2 for a minimum of 4 cycles, was administered alone (51 patients) or in association with CHT (13). RT (2) or IFN (3). Results. No patient presented adverse effects during the Rituximab infusion. A reduction of circulating B lymphocytes was observed for 11 months, on the average, without an increased risk of infections. No added toxicity was observed in patients treated with Rituximab plus CHT. Overall response rate was 97,3% (CR 82,6 %, PR 14,6 %). Five–years Overall Survival (OS) was 86,9% with Disease Free Survival of 57%. According to stage OS was in T1 94,3%, in T2 90,5%, in T3 73,6%. (T1+T2 vs T3: p Conclusions. Rituximab is effective and safe in the treatment of PCBCL even in heavily-treated or elderly patients. In our patients only the stage of disease was significant for the prognosis. A higher number of patients are necessary to indicate Rituximab in biological and clinical subsets of patients as a front-line therapy. Disclosures No relevant conflicts of interest to declare.
- Published
- 2014
50. Mycosis Fungoides in the Setting of T-cell Large Granular Lymphocyte Proliferative Disorder
- Author
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Alessandro Di Stefani, Sergio Chimenti, Lucia Anemona, Rosita Saraceno, and Andrea Saggini
- Subjects
Leukemia ,Mycosis fungoides ,Pathology ,medicine.medical_specialty ,T-Cell Large Granular Lymphocyte ,business.industry ,Medicine ,Dermatology ,General Medicine ,business ,medicine.disease ,Ultraviolet therapy - Published
- 2012
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