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The aim of this study was to evaluate whether the RI and PI values would help in choosing the best embryo recipient, and observe whether CL vascularization would influence P4 production. During the breeding season 2018/2019, the study was conducted using 35 mares, which is used for reference to collect data for the project on the day of embryo transfer. The utilized mares were divided into five groups followed by the day after ovulation, with D0 being the day of ovulation. Therefore, the five groups are as follows: D4-mares that were on the 4th post-ovulation day; D5-mares that were on the 5th post-ovulation day; and doing so successively for D6, D7 and D8. On the day of embryo transfer, the CL of the mares that selected as recipients was evaluated by B-mode and power flow mode ultrasonography and the right and left dorsal branches of the uterine arteries by spectral Doppler ultrasonography. Blood samples were taken on the day of the embryo transfer for a dosage of P4 concentration by radioimmunoassay. No statistical difference was found between the variables when the mares were separated into pregnant and non-pregnant mares, or when they were separated by age groups. When the groups of mares were compared by the day of embryo transfer, the statistical difference was found between the groups D5 × D6 (p = .0053) and D6 × D8 (p = .0036) in RI variable. In PI variable, the statistical difference was found between the groups D4 × D8 (p = .049), D5 × D6 (p = .0446) and D6 × D8 (p = .0024). We conclude that the mares with RI measurement of uterine arteries near 1.0 are correlated to mares with high CL vascularization and elevated P4 concentration.

The aim of this study was to evaluate the impact of successive bovine testicular punctures using different needle sizes. Fifteen bulls were submitted to testicular needle aspiration (TNA) in the left and right testis using 18-gauge (40×12mm) or 22-gauge (25×7mm) needles, respectively, once every 30 days. Animals were randomly divided into three groups, which were submitted to bilateral orchiectomy two days after the last puncture. Group 1 (G1): only one puncture (n=5); Group 2 (G2): three consecutive punctures in a period of three months (n=5); Group 3 (G3): six consecutive punctures in a period of 6 months (n=5). Fragments from the medial portion of the testicular parenchyma were excised and fixed in Bouin’s fluid for histological analysis. No differences were observed in the percentage of seminiferous tubules degeneration between G1, G2 and G3 (P>0.05). Higher amounts of erythrocyte were found in G1 and G2 groups compared to G3, in the intra- and intertubular tissue (P

Made available in DSpace on 2019-10-06T16:57:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-02-01 The aim of this study was to investigate the influence of sperm concentration, sperm quality, and semen deposition site on uterine inflammatory response in mares. Twenty-five mares with healthy reproductive tracts were used for artificial inseminations (AIs) using frozen-thawed semen and divided into 5 experimental groups: Control—insemination with 800 × 106 total spermatozoa in the body of the uterus (n = 5); G2—insemination with 40 × 106 total motile sperm (TMS) into uterine body (n = 5); G3—insemination with 40 × 106 TMS at the uterotubal junction by hysteroscopy (HI) (n = 5); G4—insemination with 40 × 106 TMS at the uterotubal junction by HI, with frozen-thawed semen selected by Percoll gradient (n = 5); G5—insemination with 40 × 106 total motility sperm into uterine body, using frozen-thawed semen selected by Percoll gradient (n = 5). Number of polymorphonuclear neutrophils in uterine cytology was observed at 8 and 24 hours after AI. A reduced inflammatory endometrial reaction (P .05) regardless of the type of insemination (conventional or hysteroscopic). In addition, mares inseminated by hysteroscopy showed a greater local inflammatory reaction (P

Resumo Dentre os diferentes fatores etiológicos, a endometrite fúngica representa uma causa frequente de infertilidade na égua. Nesse sentido, dois experimentos foram realizados para avaliar a sensibilidade do Dermatobac®, sistema originalmente desenvolvido para o isolamento de fungos de interesse em Medicina Humana. No Experimento 1, cepas padrão de Candida (n=4) e Aspergyllus (n=1) foram semeadas assepticamente e em duplicata no sistema Dermatobac®, acompanhando-se o crescimento fúngico a cada 24 horas por 7 dias. Para o Experimento 2, foram selecionadas 34 éguas, submetidas à colheita de material uterino para o plaqueamento em Dermatobac® e realização de esfregaços para pesquisa direta de fungos (Controle). No Experimento 1, foi possível a observação macroscópica de todas as cepas de Candida e Aspergyllus a partir de 24 horas do início do cultivo microbiológico. No Experimento 2, foi constatada a presença de leveduras nas lâminas citológicas de cinco animais (14,71%), resultados também confirmados na semeadura em Dermatobac® (sensibilidade de 100%). Foram observados cinco casos falso-positivos caracterizados pelo crescimento microbiológico em sistema Dermatobac® não confirmados através da citologia endometrial, resultados que determinaram a especificidade de 85%. No entanto, todos os casos falso-positivos foram atribuídos a microrganismos contaminantes que apresentaram morfologia diferenciada e tempo de crescimento superior (P

Objective: The castration of male beef cattle is a routine practice in many bovine herds, but this procedure is stressful and affects profitability by reducing average of weight daily gain and increasing susceptibility to diseases. The aim of this study was to evaluate the effectiveness and security of nylon cable ties of electric usage as an alternative method for hemostasis in bovine orchiectomy. Methods: For the study, 22 animals were randomly divided into 2 groups according to the hemostasis method: Control-Group (CG, n=11) using Chromic Catgut; and Nylon Cable Ties Group (CTG, n=11). For the evaluation of the hemostasis was considered the healing time, incidence of postoperative complications and the results of hematological exams performed on days 0 (D0), 2 (D2) and 7 (D7) from the postoperative period. Results: Differences were not observed when comparing the hematocrit, total protein and plasma fibrinogen of animals castrated with nylon cable ties or Catgut in any of the evaluated moments (P>0.05). CG or CTG animals did not show alterations in any of the hematological variables serially evaluated between the moments D0 and D7 (P>0.05), except for the leukocytes count which presented significant increase, regardless of the experimental group (CG, P=0.0125; CTG, P=0.0080), event related to the transitory inflammatory process as a result of surgical procedure. Conclusion: It was concluded that the use of nylon cable ties represents an economic and effective alternative for hemostasis in bovine orchiectomy.