Can Sperm Selection, Inseminating Dose, and Artificial Insemination Technique Influence Endometrial Inflammatory Response in Mares?

Made available in DSpace on 2019-10-06T16:57:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-02-01 The aim of this study was to investigate the influence of sperm concentration, sperm quality, and semen deposition site on uterine inflammatory response in mares. Twenty-five mares with healthy reproductive tracts were used for artificial inseminations (AIs) using frozen-thawed semen and divided into 5 experimental groups: Control—insemination with 800 × 106 total spermatozoa in the body of the uterus (n = 5); G2—insemination with 40 × 106 total motile sperm (TMS) into uterine body (n = 5); G3—insemination with 40 × 106 TMS at the uterotubal junction by hysteroscopy (HI) (n = 5); G4—insemination with 40 × 106 TMS at the uterotubal junction by HI, with frozen-thawed semen selected by Percoll gradient (n = 5); G5—insemination with 40 × 106 total motility sperm into uterine body, using frozen-thawed semen selected by Percoll gradient (n = 5). Number of polymorphonuclear neutrophils in uterine cytology was observed at 8 and 24 hours after AI. A reduced inflammatory endometrial reaction (P .05) regardless of the type of insemination (conventional or hysteroscopic). In addition, mares inseminated by hysteroscopy showed a greater local inflammatory reaction (P