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The present work aimed to examine the association between physical activity (PA) and sedentary behaviour with maximal fat oxidation (MFO) in young individuals. A total of 77 active adults (30 women; 22.8 ± 4.5 years) were included in this cross-sectional study in which PA and sedentary behaviour were measured using accelerometers for 7 consecutive days. PA was classified into different intensities (i.e. light, moderate, vigorous, and moderate-to-vigorous) and sedentary behaviour into sedentary time (i.e. time, and the number of bouts) and sedentary breaks (i.e. time, and the number of bouts). MFO was determined using a graded cycloergometer test through indirect calorimetry and relativized to lean mass (MFOLM) and lean leg mass (MFOLL). Positive associations were found for light and vigorous PA in relation with MFO, MFOLM and MFOLL, independently of cofounders (P≤0.01). Moreover, a negative association was found between MFO and MFOLM and the length of sedentary behaviour which was accentuated after adjusting by cardiorespiratory fitness (P≤0.05). No associations were found between sedentary time and MFOLM or MFOLL (P>0.05). These results suggest that light and vigorous PA and sedentary behaviour are related to MFO during exercise. Despite this, further interventional studies are needed to clarify if increments of light and vigorous PA could enhance MFO in different populations.

Background: According to the current physical activity (PA) recommendations, children should accumulate 60 minutes of moderate to vigorous PA (MVPA) throughout the day, 30 minutes of MVPA during the school hours, and 50% of the recess time in MVPA. Our aim was to examine the temporal trends of accelerometer-based PA during the previously mentioned day segments and the proportion of children who met the PA recommendations. Methods: This was a cross-sectional study with 2 independent samples: 499 fourth graders (49.2% females) in 2011–2012 and 364 fourth graders (46.9% females) in 2017–2018. Hip-worn accelerometers were used to assess PA. Results: A decline in light PA, moderate PA, vigorous PA, MVPA, and total PA during whole day, and in the rate of compliance with daily MVPA recommendations in males (P P P P Conclusions: Interventions are needed to attenuate the temporal decrease in PA levels in children.

The aim was to examine the bidirectional association between cardiorespiratory fitness (CRF) and fatness in a 2-year longitudinal study conducted in young people. A total of 1082 children (512 females) and 727 adolescents (342 females) with complete data at baseline and follow-up were included. CRF was determined by the 20-m shuttle run test. Height and weight were measured, and body mass index and fat mass index (kg/m2 ) were calculated. Triceps and subscapular skinfolds were assessed to compute body fat percentage. Bidirectionality was tested by including CRF and each fatness index as exposures and outcomes, alternatively, in different regression models. CRF was prospectively associated with fatness index levels (s from -0.186 to -0.528; P < .001), remaining significant in children after adjusting for baseline fatness indices. Fatness indices were prospectively associated with CRF (s from -0.207 to -0.479; P < .001), with no substantial changes observed in children and female adolescents when baseline CRF was considered. Changes in CRF were prospectively associated with several fatness indices, regardless of body fatness at baseline (s from -0.062 to -0.220; P < .05). Body fatness changes were associated with future CRF levels, independently of baseline CRF, especially in children of both sexes and male adolescents (s from -0.079 to -0.260; P < .05). Overall, a bidirectional association was observed between CRF and fatness in children and adolescents. The attainment of optimal CRF and fatness levels in early ages should be promoted since it could result in favorable future fatness and CRF levels, respectively, which are two key determinants of health status.

The aim was to determine the independent associations of muscular fitness (MF), cardiorespiratory fitness (CRF) and waist circumference (WC) with blood pressure (BP) levels over 2 years in children and adolescents.1089 children (517 females) and 787 adolescents (378 females) with complete data on fitness, WC and BP (systolic [SBP] and diastolic [DBP]) were included. Upper MF was assessed through the handgrip strength test, and lower MF using the standing long jump test. The 20-m shuttle run test was used to assess CRF. WC was obtained following standardized methods. Different regression models were fitted by introducing fitness and WC at baseline and their changes as exposures and BP at follow-up and their changes as outcomes.WC at baseline was positively and independently associated with each BP variable at follow-up in children and adolescents (β=0.094-0.260; p≤0.05), and CRF was negatively associated with DBP in adolescents (β=-0.096; p=0.034). WC changes were associated with BP variables 2 years later in children (β=0.121-0.142; p0.01). In adolescents, changes in upper MF (β=-0.116; p=0.001) and WC (β=0.080-0.098; p0.05) were associated with SBP at follow-up. WC changes were independently associated with changes in each BP variable in children (β=0.111-0.145; all p0.05) and SBP changes in adolescents (β=0.103 to 0.117; all p0.01).WC, but neither MF nor CRF, is independently associated with BP and its changes over 2 years. The attainment or maintenance of optimal fatness levels in the pediatric population should be highly encouraged for the prevention of future hypertension.

New findings What is the central question of this study? In some studies, biopsies have been performed of the subcutaneous adipose tissue in the abdomen, and they failed to find browning markers. Is the abdomen the right place to take biopsies? What is the main finding and its importance? For first time, we observed that the glucose uptake in the dorsocervical subcutaneous adipose tissue is higher in comparison to other areas of subcutaneous adipose tissue. Abstract Neonates have subcutaneous brown adipose tissue (BAT) in the dorsocervical area, and it is thought that these depots gradually disappear with age. Here, we determined that young adults have high 18 F-flurodeoxyglucose (18 F-FDG) uptake in the subcutaneous adipose tissue (SAT) of the dorsocervical area. A total of 133 young adults (age 22 ± 2 years; body mass index 25 ± 5 kg m2 ) were included in the study. We performed a shivering threshold test for every participant. Later, we performed 2 h of personalized cold exposure, immediately before a positron emission tomography/computed tomography scan. We showed that 23 of 133 participants had 18 F-FDG uptake in the dorsocervical area that achieved the criteria to be considered BAT, mainly in women (96%, n = 22 of 23). In the whole sample, the glucose uptake in the SAT of the dorsocervical area was positively correlated with BAT volume and activity located in the supraclavicular area. We showed that the 18 F-FDG uptake of the SAT of the dorsocervical area in humans is different from that of other SAT areas. Future studies are warranted to confirm the brown signature of this tissue.

The aims of the present study were: i) to examine the associations of total accelerometer-based sedentary time (ST) and specific-domain self-reported ST (i.e., screen-based, educational-based, social-based, and other-based ST) with adiposity and physical fitness in youth; and ii) to analyse the mediation effect of physical activity (PA) on associations. This study was conducted with 415 children (9.1 ± 0.4 years) and 853 adolescents (13.6 ± 1.6 years) in Spain during 2011-2012. Total ST and PA were assessed by accelerometry. Leisure-time spent in twelve sedentary behaviours was self-reported. Adiposity and physical fitness was measured following the ALPHA battery for youth. Total accelerometer-based ST was positively associated with global adiposity score in children, and negatively associated with global physical fitness score in children and adolescents; but relationships were not independent of PA. PA mediated all associations of accelerometer-based and self-reported ST with adiposity or physical fitness in children. Conversely, screen-, educational-, social-, and other-based ST were negatively related to physical fitness in adolescents, independently of PA. These findings give an impetus to developing effective strategies for specifically promoting PA in children and for increasing PA while reducing ST in adolescents in order to produce improvements on adiposity and physical fitness.

[Background]: The main objective of this study was to describe the inflammatory status of adolescents with Down syndrome (DS) and their relationship with adiposity., [Methods]: Ninety‐five adolescents with DS (44.2% girls) and a control group of 113 adolescents (47.8% girls), aged between 11 and 18 years old, from the UP & DOWN study were included in this substudy. Serum C‐reactive protein, C3 and C4 complement factors, total proteins, interleukin‐6, tumour necrosis factor‐α, insulin, cortisol, leptin, adiponectin, galactin‐3 and visfatin were analysed; homeostatic model assessment index was calculated. In order to evaluate adiposity, we measured the following body fat variables: weight, height, waist circumference and skinfold thicknesses. Birth weight was obtained by questionnaire. In addition, body mass index, waist‐to‐height ratio (WHtR) and body fat percentage (BF%) were calculated., [Results]: Down syndrome group showed higher levels of body mass index, WHtR, waist circumference, BF% and lower birth weight than controls (P < 0.001). In the general linear model in the total sample, WHtR was positively associated with C3 and C4 (P < 0.001) as well as with leptin levels (P = 0.015). BF% was positively associated with total proteins (P = 0.093) and leptin levels (P < 0.001). DS was positively associated with total proteins (P < 0.001), C3 (P = 0.047) and C4 (P = 0.019). Despite the higher levels of adiposity found in DS group, no direct association was found between BF% and leptin levels, comparing with the control group., [Conclusions]: These findings suggest that abdominal obesity should be controlled in adolescents because of its relationship with acute phase‐inflammatory biomarkers but especially in DS adolescents who may show a peculiar metabolic status according to their relationship between adiposity and inflammatory biomarkers., We appreciate the following financial support: DEP 2010‐21662‐C04‐00 grants from the National Plan for Research, Development and Innovation (R + D + i) MICINN.

Introduction Unhealthy lifestyle and inadequate diet could influence the development of future cardiometabolic disease. The main aim of this study was to determine the association between aerobic fitness and cardiometabolic risk factors in adults, whether this relation is depends of adherence to Mediterranean diet (MD). A secondary aim was to study the combined effect of aerobic capacity and adherence to MD on global cardiometabolic risk score (CMRS). Method A total of 79 adults (38% women) enrolled between 18–40 year from Cadiz. We measured adiposity indicators, blood pressure, triglycerides, glucose and inflammatory profile (interleukin-6 and tumor necrosis factor) and was computed (CMRS). Aerobic fitness was measured by maximal oxygen comsuption through an incremental stress test by cycleergometer. The MD patterns was measured using the questionnaire of adherence to MD. The association between aerobic fitness and cardiometabolic risk factors was examined using a lineal regression and it was adjusted for different confounders. CMRS on the lifestyle was analyzed using the ANOVA test, with statistical significance level of p Results Linear regression showed inverse association between aerobic fitness and cardiometabolic risk factors (all p ≤ 0.05) in the model without adjustment. Blood pressure and triglycerides lost the association after adjust model for sex, age, and adherence to MD. Participants with high aerobic fitness and high adherence to MD show a lowest CMRS (−1.083 ± 2.325 vs. 2.802 ± 1.759). Conclusions Aerobic fitness was inversely associated with fatness risk factors, that relationship is independent to adherence to MD. A high adherence to MD could modulate blood pressure. A combination of high aerobic capacity and high adherence to MD could reduce the adverse consecuence of a low adherencie to MD.

We are grateful to Ms. Carmen Sainz-Quinn for assistance with the English language. We are grateful to Alberto Quesada-Aranda for helping with the development of the Temperatus software (Free trial in http://profth.ugr.es/temperatus)., This study is part of a Ph.D. Thesis conducted in the Biomedicine Doctoral Studies of the University of Granada, Spain., Brown adipose tissue (BAT) thermogenic activity is commonly assessed with a positron emission tomography with computed tomography scan (PET/CT). This technique has several limitations and alternative techniques are needed. Supraclavicular skin temperature measured with iButtons and infrared thermography (IRT) has been proposed as an indirect marker of BAT activity. We studied the concurrent validity of skin temperature measured with iButtons vs. IRT and the association of supraclavicular skin temperature measured with iButtons and IRT with BAT. We measured skin temperature upon a shivering threshold test with iButtons and IRT in 6 different regions in 12 participants (n = 2 men). On a separate day, we determined supraclavicular skin temperature with an iButton and IRT after 2 h of a personalized cooling protocol. Thereafter, we quantified BAT volume and activity by PET/CT. We observed that the absolute differences between the devices were statistically different from 0 (all P, This study was supported by the Spanish Ministry of Economy and Competitiveness via the Fondo de Investigación Sanitaria del Instituto de Salud Carlos III (PI13/01393), Retos de la Sociedad (DEP2016-79512-R), PTA 12264-I, and European Regional Development Fund (ERDF), the Spanish Ministry of Education (FPU 13/04365, FPU14/04172, FPU15/05337, and FPU15/04059), by the Spanish Ministry of Science and Innovation-MINECO (RYC-2014-16938), the Fundación Iberoamericana de Nutrición (FINUT), the Redes Temáticas de Investigación Cooperativa RETIC (Red SAMID RD16/0022), the AstraZeneca HealthCare Foundation, the University of Granada Plan Propio de Investigación 2016 -Excellence actions: Unit of Excellence on Exercise and Health (UCEES) - and Plan Propio de Investigación 2018 - Programa Contratos-Puente, and the Junta de Andalucía, Consejería de Conocimiento, Investigación y Universidades (ERDF, ref. SOMM17/6107/UGR). This study is part of a Ph.D.

[Background]: The combined effect of cardiorespiratory fitness (CRF) and body mass index (BMI) on cardiovascular disease (CVD) risk in young people remains to be fully determined. We examined the individual and combined associations of CRF and BMI with clustered CVD risk factors, and the mediator role of BMI in the association between CRF and clustered CVD risk factors in children and adolescents., [Methods]: 237 children (111 girls) and 260 adolescents (120 girls) were included in this cross‐sectional study. Height and weight were assessed and BMI was calculated. A CVD risk factor index (CVDRF‐I) was computed from: waist circumference, systolic blood pressure, triglycerides, high‐density lipoprotein cholesterol and glucose. CRF was assessed using the 20‐m shuttle run test. Regression analysis, analysis of covariance and mediation analysis (Baron and Kenny procedures) were used to test the independent and combined effect of CRF and BMI on CVDRF‐I, and to test mediation hypothesis, respectively., [Results]: CRF was negatively associated with CVDRF‐I (all P, [Conclusions]: BMI is an independent predictor of CVDRF‐I and a mediator of the association between CRF and CVDRF‐I in children and adolescents. These results help to clarify the associations between CRF, weight status and cardiovascular health, suggesting that future CVD health would benefit from maintaining an optimal weight status., This work was supported by the National Plan for Research, Development and Innovation (R + D + i) from the Spanish Ministry of Science and Innovation [DEP 2010‐21662‐C04‐00 (DEP 2010‐21662‐C04‐01: DEP 2010‐21662‐C04‐02: DEP 2010‐21662‐C04‐03: DEP 2010‐21662‐C04‐04)]; and by the Spanish Ministry of Education [FPU15/05337]. IEC is supported by a grant from the Alicia Koplowitz Foundation.

UP&DOWN Study Group additional members: Coordinator: Ascension Marcos; Principal Investigators: Ascension Marcos, Jose Castro-Piñero, Oscar L. Veiga, and Fernando Bandres; Scientific Coordinators: David Martinez-Gomez (chair), Jonatan R. Ruiz (co-chair), Ana Carbonell-Baeza, Sonia Gomez-Martinez, and Catalina Santiago; Spanish National Research Council: Ascension Marcos, Sonia Gomez-Martinez, Esther Nova, Ligia-Esperanza Diaz, Belen Zapatera, Ana M. Veses, Aurora Hernandez, and Alina Gheorghe; University of Cadiz: José Castro-Piñero, Jesus Mora-Vicente, Jose L. Gonzalez-Montesinos, Julio Conde-Caveda, Jonatan R. Ruiz (University of Granada [UGR]), Francisco B. Ortega (UGR), Carmen Padilla Moledo, Ana Carbonell Baeza, Palma Chillon (UGR), Jorge del Rosario Fernandez, Ana Gonzalez Galo, Gonzalo Bellvis Guerra, Alvaro Delgado Alfonso, Fernando Parrilla, Roque Gomez, and Juan Gavala; Autonomous University of Madrid: Oscar L. Veiga, H. Ariel Villagra, Juan del-Campo, Carlos Cordente (UPM), Mario Diaz, Carlos M. Tejero, Aitor Acha, Jose M. Moya, Alberto Sanz, David Martinez-Gomez, Veronica Cabanas-Sanchez, Gabriel RodriguezRomo (UPM), Rocio Izquierdo, Laura Garcia-Cervantes, and Irene Esteban-Cornejo; Complutense University of Madrid: Fernando Bandres, Alejandro Lucia (European University of Madrid [UEM]), Catalina Santiago (UEM), and Felix Gomez-Gallego (UEM)., [Objectives]: To study the cross-sectional and longitudinal (2-year follow-up) association between muscle fitness and cardiovascular disease (CVD) risk factors in youth; whether there are muscle fitness cut points associated with CVD risk (cross-sectional); and whether the health-related muscle fitness cut points identified at baseline are associated with CVD risk 2 years later. [Study design]: In total, 237 children (110 girls) aged 6-10 years and 274 adolescents (131 girls) aged 12-16 years with complete data were included in the study (10.3% drop out). The handgrip strength and the standing long jump tests were used to assess muscle fitness. CVD risk score was computed with sum of 2 skinfolds, systolic blood pressure, insulin, glucose, triglycerides, and total cholesterol/high density lipoprotein cholesterol. [Results]: Muscle fitness at baseline was associated inversely with single CVD risk factors and CVD risk score at baseline and 2-year follow-up (all P, Supported by the DEP 2010-21662-C04-00 (DEP 2010-21662-C04-01: DEP 2010-21662-C04-02: DEP 2010-21662-C04-03: DEP 2010-21662-C04-04) grant from the National Plan for Research, Development and Innovation (R + D + i) MICINN, by the FPU15/05337 grant from the Spanish Ministry of Education, and by Plan Propio de Investigación 2016 of the University of Granada (Spain), Excellence actions: Units of Excellence; Scientific Unit of Excellence on Exercise and Health (UCEES).

[Background]: We aimed to examine the independent and combined associations of cardiorespiratory fitness, muscular fitness, and motor ability with single and clustered inflammatory biomarkers in children and adolescents., [Methods]: This study included 503 children and adolescents. Cardiorespiratory fitness, upper- and lower-muscular fitness, and motor ability were assessed using field-based tests. Fasting blood samples were obtained to determine the levels of a set of inflammatory biomarkers. Global physical fitness and clustered inflammatory biomarker scores were computed. Associations between physical fitness and inflammatory biomarkers were analyzed through linear regression. Differences in inflammatory biomarker levels between physical fitness tertiles were tested., [Results]: Global physical fitness was inversely associated with single and clustered inflammatory biomarkers in children (p, [Conclusion]: Physical fitness was negatively associated with single and clustered inflammatory biomarkers, independently of body mass index. Increasing physical fitness levels in youth might contribute to reduce the cardiovascular risk., This work was supported by the National Plan for Research, Development, and Innovation (R + D + i) from the Spanish Ministry of Science and Innovation [DEP 2010-21662-C04-00 (DEP 2010-21662-C04-01: DEP 2010-21662-C04-02: DEP 2010-21662-C04-03: DEP 2010-21662-C04-04)]; and by the Spanish Ministry of Education [FPU15/05337].

Portions of this study were presented at the 22nd Annual Congress of the European College of Sport Science, July 5-8, 2017, Essen, Germany., [Objectives]: To test the associations of muscular fitness and body mass index (BMI), individually and combined, with clustered cardiovascular disease risk factors in children and adolescents and to analyze the mediator role of BMI in the association between muscular fitness and clustered cardiovascular disease risk factors., [Study design]: In total, 239 children (113 girls) and 270 adolescents (128 girls) participated in this cross-sectional study. Height and weight were assessed, and BMI was calculated. A cardiovascular disease risk factors index (CVDRF-I) was created from the combination of the following variables: waist circumference, systolic blood pressure, triglycerides, high-density lipoprotein cholesterol, and glucose. Handgrip strength/weight and standing long jump tests were used to assess muscular fitness. A muscular fitness index was computed from the combination of both tests., [Results]: Muscular fitness index was associated with CVDRF-I in children of both sexes and adolescent boys; however, these associations disappeared after accounting for BMI. BMI was associated with CVDRF-I in both children and adolescents, even after adjusting for muscular fitness (all P, [Conclusions]: BMI is an independent predictor of CVDRF-I in children and adolescents of both sexes. Conversely, the effect of muscular fitness on CVDRF-I seems to be fully mediated by BMI levels in male and female children and in adolescent boys., Supported by the National Plan for Research, Development and Innovation (R + D + i) MICINN DEP 2010-21662-C04-00 (DEP 2010-21662-C04-01; DEP 2010-21662-C04-02; DEP 2010-21662-C04-03; DEP 2010-21662-C04-04) and by the Spanish Ministry of Education (FPU15/05337).