1. Osteostatin-loaded bioceramics stimulate osteoblastic growth and differentiation.
- Author
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Lozano D, Manzano M, Doadrio JC, Salinas AJ, Vallet-Regí M, Gómez-Barrena E, and Esbrit P
- Subjects
- 3T3 Cells, Animals, Cell Differentiation drug effects, Cell Proliferation drug effects, Coated Materials, Biocompatible chemistry, Coated Materials, Biocompatible pharmacology, Crystallization methods, Materials Testing, Mice, Osteoblasts drug effects, Osteogenesis drug effects, Osteogenesis physiology, Surface Properties, Tissue Engineering methods, Bone Substitutes chemistry, Bone Substitutes pharmacology, Ceramics chemistry, Osteoblasts cytology, Osteoblasts physiology, Parathyroid Hormone-Related Protein administration & dosage, Parathyroid Hormone-Related Protein chemistry
- Abstract
Parathyroid hormone-related protein (PTHrP) is an important regulator of bone remodeling. Recent studies show that this protein can induce osteogenic features through its N- and C-terminal domains. Silica-based ordered mesoporous bioceramics with an SBA-15 structure - known to be bioactive and biocompatible - have recently been evaluated for their capacity to uptake and deliver L-tryptophan. This amino acid corresponds to the end position of the 107-111 domain (called osteostatin) of the native C-terminal PTHrP (107-139) fragment, whose true action in bone metabolism is still ill-defined. In the present study, we assessed some effects of the aforementioned biomaterials pressed into disks, loaded or not with osteostatin, in osteoblastic cell cultures. Our data demonstrate that both unmodified and organically modified SBA-15 loaded with this peptide increase cell growth and the expression of several osteoblastic products (alkaline phosphatase, osteocalcin, collagen, osteoprotegerin, receptor activator of nuclear factor-kappaB ligand and vascular endothelial growth factor) in osteoblastic cells. These findings support the notion that osteostatin coating confers osteogenic features to silica-based ordered mesoporous materials, which make them suitable biomaterials for bone repair., (Copyright 2009 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2010
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