1. Acetylene-sourced CVD-synthesised catalytically active graphene for electrochemical biosensing.
- Author
-
Osikoya AO, Parlak O, Murugan NA, Dikio ED, Moloto H, Uzun L, Turner AP, and Tiwari A
- Subjects
- Acetylene chemistry, Aspergillus niger chemistry, Biosensing Techniques methods, Electrochemical Techniques methods, Electrodes, Equipment Design, Glucose analysis, Limit of Detection, Models, Molecular, Surface-Active Agents chemistry, Volatilization, Wettability, Aspergillus niger enzymology, Biosensing Techniques instrumentation, Electrochemical Techniques instrumentation, Enzymes, Immobilized chemistry, Glucose Oxidase chemistry, Graphite chemistry
- Abstract
In this study, we have demonstrated the use of chemical vapour deposition (CVD) grown-graphene to develop a highly-ordered graphene-enzyme electrode for electrochemical biosensing. The graphene sheets were deposited on 1.00mm thick copper sheet at 850°C using acetylene (C
2 H2 ) as carbon source in an argon (Ar) and nitrogen (N2 ) atmosphere. An anionic surfactant was used to increase wettability and hydrophilicity of graphene; thereby facilitating the assembly of biomolecules on the electrode surface. Meanwhile, the theoretical calculations confirmed the successful modification of hydrophobic nature of graphene through the anionic surface assembly, which allowed high-ordered immobilisation of glucose oxidase (GOx) on the graphene. The electrochemical sensing activities of the graphene-electrode was explored as a model for bioelectrocatalysis. The bioelectrode exhibited a linear response to glucose concentration ranging from 0.2 to 9.8mM, with sensitivity of 0.087µA/µM/cm2 and a detection limit of 0.12µM (S/N=3). This work sets the stage for the use of acetylene-sourced CVD-grown graphene as a fundamental building block in the fabrication of electrochemical biosensors and other bioelectronic devices., (Copyright © 2016 Elsevier B.V. All rights reserved.)- Published
- 2017
- Full Text
- View/download PDF