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Ultrasensitive detection of human liver hepatocellular carcinoma cells using a label-free aptasensor.
- Source :
-
Analytical chemistry [Anal Chem] 2014 May 20; Vol. 86 (10), pp. 4956-60. Date of Electronic Publication: 2014 May 08. - Publication Year :
- 2014
-
Abstract
- Liver cancer is one of the most common cancers in the world and has no effective cure, especially in later stages. The development of a tangible protocol for early diagnosis of this disease remains a major challenge. In the present manuscript, an aptamer-based, label-free electrochemical biosensor for the sensitive detection of HepG2, a hepatocellular carcinoma cell line, is described. The target cells are captured in a sandwich architecture using TLS11a aptamer covalently attached to a gold surface and a secondary TLS11a aptamer. The application of TLS11a aptamer as a recognition layer resulted in a sensor with high affinity for HepG2 cancer cells in comparison with control cancer cells of human prostate, breast, and colon tumors. The aptasensor delivered a wide linear dynamic range over 1 × 10(2) to 1 × 10(6) cells/mL, with a detection limit of 2 cells/mL. This protocol provides a precise method for sensitive detection of liver cancer with significant advantages in terms of simplicity, low cost, and stability.
Details
- Language :
- English
- ISSN :
- 1520-6882
- Volume :
- 86
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Analytical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 24754473
- Full Text :
- https://doi.org/10.1021/ac500375p