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1. A SOE-PCR method of introducing multiple mutations into Mycoplasma gallisepticum neuraminidase

Author

Suibin He, Xusheng Qiu, Shilei Zhang, Meirong Hu, Danqing Chen, Cuiping Song, Lei Tan, Hongjun Chen, Xinyue Shen, Fanqing Zhang, Chan Ding, and Shengqing Yu

Subjects
Microbiology (medical), Mycoplasma gallisepticum, biology, Neuraminidase, biology.organism_classification, Microbiology, Virology, Polymerase Chain Reaction, law.invention, Bacterial Proteins, law, RNA splicing, biology.protein, Recombinant DNA, Mutagenesis, Site-Directed, Point Mutation, Pcr method, Overlap extension polymerase chain reaction, Molecular Biology, Gene
Abstract

A modified splicing with overlap extension PCR (SOE-PCR) was generated to introduce 21 TGA to TGG at Mycoplasma gallisepticum MGA_0329 gene. The recombinant protein was successfully expressed and retained neuraminidase activities, indicating that SOE-PCR is a rapid and highly efficient method of introducing multiple mutations into large M. gallisepticum genes.

Published
2013

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