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A SOE-PCR method of introducing multiple mutations into Mycoplasma gallisepticum neuraminidase
- Source :
- Journal of microbiological methods. 94(2)
- Publication Year :
- 2013
-
Abstract
- A modified splicing with overlap extension PCR (SOE-PCR) was generated to introduce 21 TGA to TGG at Mycoplasma gallisepticum MGA_0329 gene. The recombinant protein was successfully expressed and retained neuraminidase activities, indicating that SOE-PCR is a rapid and highly efficient method of introducing multiple mutations into large M. gallisepticum genes.
- Subjects :
- Microbiology (medical)
Mycoplasma gallisepticum
biology
Neuraminidase
biology.organism_classification
Microbiology
Virology
Polymerase Chain Reaction
law.invention
Bacterial Proteins
law
RNA splicing
biology.protein
Recombinant DNA
Mutagenesis, Site-Directed
Point Mutation
Pcr method
Overlap extension polymerase chain reaction
Molecular Biology
Gene
Subjects
Details
- ISSN :
- 18728359
- Volume :
- 94
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of microbiological methods
- Accession number :
- edsair.doi.dedup.....fb30af580f8e1e21a42af4e13f44f0d7