1. Biosynthesis of cell wall mannan in the conidium and the mycelium ofAspergillusfumigatus
- Author
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Yanan Zhao, Anne Beauvais, David S. Perlin, Marie-Christine Prévost, Rémi Beau, Thierry Fontaine, Vishukumar Aimanianda, Jean-Paul Latgé, Christine Henry, Pauline Robinet, Arnaud Fekkar, Christoph Heddergott, and Isabelle Mouyna
- Subjects
0301 basic medicine ,biology ,Immunology ,Fungal genetics ,Mannose ,Mannosyltransferases ,bacterial infections and mycoses ,biology.organism_classification ,Microbiology ,Yeast ,Aspergillus fumigatus ,carbohydrates (lipids) ,Cell wall ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,chemistry ,Biochemistry ,Virology ,skin and connective tissue diseases ,Mycelium ,Mannan - Abstract
The galactomannan is a major cell wall molecule of Aspergillus fumigatus. This molecule is composed of a linear mannan with a repeating unit composed of four α1,6 and α1,2 linked mannose with side chains of galactofuran. To obtain a better understanding of the mannan biosynthesis in A. fumigatus, it was decided to undertake the successive deletion of the 11 genes which are putative orthologs of the mannosyltransferases responsible for establishing α1,6 and α1,2 mannose linkages in yeast. These deletions did not lead to a reduction of the mannan content of the cell wall of the mycelium of A. fumigatus. In contrast, the mannan content of the conidial cell wall was reduced and this reduction was associated with a partial disorganization of the cell wall leading to defects in conidial survival both in vitro and in vivo.
- Published
- 2016