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A unique virus release mechanism in the Archaea
- Source :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2009, epub ahead of print. ⟨10.1073/pnas.0901238106⟩, Proceedings of the National Academy of Sciences of the United States of America, 2009, epub ahead of print. ⟨10.1073/pnas.0901238106⟩
- Publication Year :
- 2009
- Publisher :
- HAL CCSD, 2009.
-
Abstract
- Little is known about the infection cycles of viruses infecting cells from Archaea, the third domain of life. Here, we demonstrate that the virions of the archaeal Sulfolobus islandicus rod-shaped virus 2 (SIRV2) are released from the host cell through a mechanism, involving the formation of specific cellular structures. Large pyramidal virus-induced protrusions transect the cell envelope at several positions, rupturing the S-layer; they eventually open out, thus creating large apertures through which virions escape the cell. We also demonstrate that massive degradation of the host chromosomes occurs because of virus infection, and that virion assembly occurs in the cytoplasm. Furthermore, intracellular viral DNA is visualized by flow cytometry. The results show that SIRV2 is a lytic virus, and that the host cell dies as a consequence of elaborated mechanisms orchestrated by the virus. The generation of specific cellular structures for a distinct step of virus life cycle is known in eukaryal virus-host systems but is unprecedented in cells from other domains.
- Subjects :
- Archaeal Viruses
Time Factors
viruses
Virus
Chromosomes
Sulfolobus
03 medical and health sciences
Viral life cycle
[SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology
030304 developmental biology
Cell Proliferation
0303 health sciences
Multidisciplinary
biology
030306 microbiology
Biological Sciences
biology.organism_classification
Flow Cytometry
Virology
Virus Release
Cell biology
Kinetics
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Lytic cycle
Virion assembly
Rudivirus
Subjects
Details
- Language :
- English
- ISSN :
- 00278424 and 10916490
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America, Proceedings of the National Academy of Sciences of the United States of America, National Academy of Sciences, 2009, epub ahead of print. ⟨10.1073/pnas.0901238106⟩, Proceedings of the National Academy of Sciences of the United States of America, 2009, epub ahead of print. ⟨10.1073/pnas.0901238106⟩
- Accession number :
- edsair.doi.dedup.....27c53afd6463a8eb56c10e0c142c5af3