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2 results on '"test cutané"'

1. Mosquito Feeding Assays to Determine the Infectiousness of Naturally Infected Plasmodium falciparum Gametocyte Carriers

Author

Yimin Wu, Ashley J. Birkett, Patricia M. Graves, Mouctar Diallo, Louis Clément Gouagna, Thomas S. Churcher, Merribeth J. Morin, Mamadou B. Coulibaly, Bert Mulder, Teun Bousema, Robert W. Sauerwein, Chris Drakeley, Ogobara K. Doumbo, Will Roeffen, Rhoel R. Dinglasan, Isabelle Morlais, Vincent Robert, Yeya T. Touré, G. A. T. Targett, Parfait Awono-Ambene, Emily Locke, Timoléon Tchuinkam, Sarah Bonnet, Colin J. Sutherland, Travis van Warmerdam, London School of Hygiene and Tropical Medicine (LSHTM), Radboud university [Nijmegen], Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), Institut de Recherche Agricole pour le Développement [Yaoundé] (IRAD), Institut de Recherche pour le Développement, Partenaires INRAE, Institut de Recherche pour le Développement (IRD), Malaria Res Inst, Biologie Moléculaire et Immunologie Parasitaires et Fongiques, École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Université des sciences, des techniques et des technologies de Bamako (USTTB), Laboratorium Microbiologie Twente, James Cook University (JCU), PATH Malaria Vaccine Initiat, NIAID, Imperial College London, Bill & Melinda Gates Foundation, European FP7 framework (REDMAL) [242079], PATH Malaria Vaccine Initiative (MVI), MVI, Bloomberg Family Foundation, Johns Hopkins Malaria Research Institute, Institut de Recherche pour le Developpement, TMRC, NIAID/NIH, Division of Intramural Research, National Institute of Allergy and Infectious Diseases, EC FP7 Collaborative project TransMalariaBloc [HEALTH-F3-2008-223736], Radboud University [Nijmegen], École nationale vétérinaire - Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)-Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), and Université des Sciences, des Techniques et des Technologies de Bamako (USTTB)

Subjects
Male, moustique, Epidemiology, [SDV]Life Sciences [q-bio], lcsh:Medicine, test cutané, Skin infection, Plasmodium, 0302 clinical medicine, Poverty-related infectious diseases Infection and autoimmunity [N4i 3], Blood plasma, Malaria, Falciparum, lcsh:Science, Child, Whole blood, 0303 health sciences, Multidisciplinary, biology, Transmission (medicine), Anopheles, Middle Aged, maladie parasitaire, 3. Good health, skin infections, Infectious Diseases, Child, Preschool, Medicine, Female, Research Article, Adult, Adolescent, Plasmodium falciparum, 030231 tropical medicine, malaria, gametocyte, Microbiology, Infectious Disease Epidemiology, Young Adult, 03 medical and health sciences, parasitic diseases, Parasitic Diseases, medicine, Gametocyte, sang, Animals, Humans, Biology, 030304 developmental biology, Population Biology, lcsh:R, Infant, plasma sanguin, medicine.disease, biology.organism_classification, Virology, infection, Insect Vectors, paludisme, Immunology, lcsh:Q, Parasitology, Malaria
Abstract

Contains fulltext : 108727.pdf (Publisher’s version ) (Open Access) INTRODUCTION: In the era of malaria elimination and eradication, drug-based and vaccine-based approaches to reduce malaria transmission are receiving greater attention. Such interventions require assays that reliably measure the transmission of Plasmodium from humans to Anopheles mosquitoes. METHODS: WE COMPARED TWO COMMONLY USED MOSQUITO FEEDING ASSAY PROCEDURES: direct skin feeding assays and membrane feeding assays. Three conditions under which membrane feeding assays are performed were examined: assays with i) whole blood, ii) blood pellets resuspended with autologous plasma of the gametocyte carrier, and iii) blood pellets resuspended with heterologous control serum. RESULTS: 930 transmission experiments from Cameroon, The Gambia, Mali and Senegal were included in the analyses. Direct skin feeding assays resulted in higher mosquito infection rates compared to membrane feeding assays (odds ratio 2.39, 95% confidence interval 1.94-2.95) with evident heterogeneity between studies. Mosquito infection rates in membrane feeding assays and direct skin feeding assays were strongly correlated (p

Published
2012

2. Thermal processing and allergenic potential of egg for children

Author

Chantal Brossard, Marie‐Anne Legoux, Julie Chabauty, Fabienne Rancé, Agnès Juchet, Martine Drouet, Evelyne Paty, Sandrine Legoué‐Morillon, Françoise Nau, Olivier Tranquet, Unité de recherche sur les Biopolymères, Interactions Assemblages (BIA), Institut National de la Recherche Agronomique (INRA), Pédiatrie-pneumologie, allergologie, Centre Hospitalier Universitaire de Toulouse, Pédiatrie, pneumologie, allergologie, Département pneumologie, unité allergologie générale, Centre Hospitalier Universitaire d'Angers (CHU Angers), PRES Université Nantes Angers Le Mans (UNAM)-PRES Université Nantes Angers Le Mans (UNAM), Pneumo Allergologie Infantile, CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Centre de recherche et d'éducation en allergie alimentaire, Centre hospitalier universitaire de Nantes (CHU Nantes), Science et Technologie du Lait et de l'Oeuf (STLO), Institut National de la Recherche Agronomique (INRA)-AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), ANR-07-PNRA-023 OVONUTRIAL, and The European Academy of Allergy and Clinical Immunology (EAACI). Zurich, CHE.

Subjects
Pulmonary and Respiratory Medicine, Allergy, food.ingredient, Immunology, Ingénierie des aliments, Pasteurization, test cutané, Immunoglobulin E, law.invention, Toxicology, 030207 dermatology & venereal diseases, 03 medical and health sciences, 0302 clinical medicine, food, law, Food allergy, Yolk, [SDV.IDA]Life Sciences [q-bio]/Food engineering, medicine, Immunology and Allergy, Food engineering, Food science, Food allergens, protéine de choc thermique, allergie alimentaire, analyse in vivo, biology, medicine.disease, réaction allergique, food.food, allergène, ovotransferrine, ovalbumine, 030228 respiratory system, Boiled egg, Poster Presentation, embryonic structures, nutrition infantile, oeuf, biology.protein, anticorps ige, Egg white, traitement thermique
Abstract

Egg belongs to the list of ingredients that must be labeled because of its frequent involvement in food allergies especially in childhood. Several thermal processes are commonly applied to egg and it is known that some egg-allergic people can tolerate well-cooked egg. Food allergens are characterized by their ability to elicit IgE antibodies (sensitization) in susceptible individuals and to display several IgE-binding epitopes. Allergenic potential influenced by different structural levels (sequence, 2D/3D structures as well as supra-molecular organization) can be greatly impacted by all modifications of these structures due to processing. Our work aimed to analyze the impact of pasteurization and boiling of egg and its fractions on in vivo reactivity and its relation to IgE-binding profiles to egg allergens/fractions in a cohort of children allergic to egg. Allergic children (n=52, 18 months - 7 years old) were recruited, all had positive skin prick-tests (SPT) to raw whole egg and egg white and 80% to raw egg yolk. Reactivity to pasteurized and boiled fractions was evaluated by SPT. Sera samples taken at inclusion were used to draw profiles of IgE-reactivity against known allergens and proteins of egg white and against fractions of egg yolk by ELISA. Relative STP data and IgE-binding profiles were analyzed by hierarchical classification to evidence typical profiles of reactivity in this cohort. Pasteurization had very limited impact on structure of egg and only 10% and 5% of children STP became negative with pasteurized (66°C, 6 min) whole egg or egg white. Boiling (100°C, 10 min) greatly changed structure of egg products and had a higher effect than pasteurization since 55%, 27% and 67% of children SPT remained positive to boiled egg and yolk and white fractions. Two typical STP behaviors were evidenced by classification in this cohort. IgE-binding profiles largely differed among children but three typical profiles where extracted from data. Contingency analysis revealed links between typical STP and IgE-binding profiles with possible explanation of tolerance to cooked egg.

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