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Mosquito Feeding Assays to Determine the Infectiousness of Naturally Infected Plasmodium falciparum Gametocyte Carriers
- Source :
- PLoS One, 7, PLoS ONE, PLoS ONE, Public Library of Science, 2012, 7 (8), ⟨10.1371/journal.pone.0042821⟩, Plos One 8 (7), . (2012), PLoS ONE; Vol 7, PLoS ONE, 2012, 7 (8), ⟨10.1371/journal.pone.0042821⟩, PLoS One, 7, 8, PLoS ONE, Vol 7, Iss 8, p e42821 (2012)
- Publication Year :
- 2012
-
Abstract
- Contains fulltext : 108727.pdf (Publisher’s version ) (Open Access) INTRODUCTION: In the era of malaria elimination and eradication, drug-based and vaccine-based approaches to reduce malaria transmission are receiving greater attention. Such interventions require assays that reliably measure the transmission of Plasmodium from humans to Anopheles mosquitoes. METHODS: WE COMPARED TWO COMMONLY USED MOSQUITO FEEDING ASSAY PROCEDURES: direct skin feeding assays and membrane feeding assays. Three conditions under which membrane feeding assays are performed were examined: assays with i) whole blood, ii) blood pellets resuspended with autologous plasma of the gametocyte carrier, and iii) blood pellets resuspended with heterologous control serum. RESULTS: 930 transmission experiments from Cameroon, The Gambia, Mali and Senegal were included in the analyses. Direct skin feeding assays resulted in higher mosquito infection rates compared to membrane feeding assays (odds ratio 2.39, 95% confidence interval 1.94-2.95) with evident heterogeneity between studies. Mosquito infection rates in membrane feeding assays and direct skin feeding assays were strongly correlated (p
- Subjects :
- Male
moustique
Epidemiology
[SDV]Life Sciences [q-bio]
lcsh:Medicine
test cutané
Skin infection
Plasmodium
0302 clinical medicine
Poverty-related infectious diseases Infection and autoimmunity [N4i 3]
Blood plasma
Malaria, Falciparum
lcsh:Science
Child
Whole blood
0303 health sciences
Multidisciplinary
biology
Transmission (medicine)
Anopheles
Middle Aged
maladie parasitaire
3. Good health
skin infections
Infectious Diseases
Child, Preschool
Medicine
Female
Research Article
Adult
Adolescent
Plasmodium falciparum
030231 tropical medicine
malaria
gametocyte
Microbiology
Infectious Disease Epidemiology
Young Adult
03 medical and health sciences
parasitic diseases
Parasitic Diseases
medicine
Gametocyte
sang
Animals
Humans
Biology
030304 developmental biology
Population Biology
lcsh:R
Infant
plasma sanguin
medicine.disease
biology.organism_classification
Virology
infection
Insect Vectors
paludisme
Immunology
lcsh:Q
Parasitology
Malaria
Subjects
Details
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS One, 7, PLoS ONE, PLoS ONE, Public Library of Science, 2012, 7 (8), ⟨10.1371/journal.pone.0042821⟩, Plos One 8 (7), . (2012), PLoS ONE; Vol 7, PLoS ONE, 2012, 7 (8), ⟨10.1371/journal.pone.0042821⟩, PLoS One, 7, 8, PLoS ONE, Vol 7, Iss 8, p e42821 (2012)
- Accession number :
- edsair.doi.dedup.....a121ea62038a0d5c68c4baba63b9f981