1. Particle Bombardment of Ex Vivo Skin to Deliver DNA and Express Proteins
- Author
-
Sokol, Ena, Nijenhuis, Miranda, Sjollema, Klaas A, Jonkman, Marcel F, Pas, Hendri H, Giepmans, Ben N G, Clausen, Björn E., Laman, Jon D., Translational Immunology Groningen (TRIGR), Microbes in Health and Disease (MHD), Center for Liver, Digestive and Metabolic Diseases (CLDM), and Basic and Translational Research and Imaging Methodology Development in Groningen (BRIDGE)
- Subjects
0301 basic medicine ,Human skin ,Transfection ,Biology ,Molecular biology ,Green fluorescent protein ,030207 dermatology & venereal diseases ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,Plasmid ,chemistry ,Live cell imaging ,Complementary DNA ,Biophysics ,Ex vivo ,DNA - Abstract
Particle bombardment of gold microparticles coated with plasmids, which are accelerated to high velocity, is used for transfection of cells within tissue. Using this method, cDNA encoding proteins of interest introduced into ex vivo living human skin enables studying of proteins of interest in real time. Here, technical aspects of particle bombardment of ex vivo skin are described using green fluorescent protein (GFP) as readout for efficiency. This method can be applied on numerous tissues, including in living model animals.
- Published
- 2017