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Efforts to reduce organic sulfur in coal are taken through biodesulfurization by using desulfurization bacteria to release covalently-bound sulfur from the coal matrix. Coal is a complex hydrocarbon material that requires collaboration from more than one type of bacteria in a consortium for desulfurization. The current study shows how the individual members of a bacterial consortium obtained directly from coal samples grew on the coal. Mineral medium containing sub-bituminous coal with a concentration of 10%, 15%, and 20% served as a carbon source and the only sulfur to support the consortium's growth. The examination included growth patterns, concentrations of dibenzothiophene as an organic sulfur representative, pH, and sulfate concentration as the sulfur product released into the medium. The growth of individual members of the consortium was observed for 336 h. The consortium grew in all three coal concentrations with slightly different cell growth patterns and the release of dibenzothiophene. Members of the consortium grew alternately and overlapped, which showed possible linkages or dependence on products and existence from the growth of other members. The existence of the primary strain Moraxella osloensis COK1 indicated that they played a role in the activities and growth of other members. The alternating growth is discussed to produce a hypothetical illustration of how several other members play in using sulfur in a well-known desulfurization pathway. In conclusion, this study provides a deeper insight into the value of consortium members individually but growing together while swarming coal as a complex resource to become low-sulfur coal.

Tanaman cabai merupakan jenis sayuran penting yang banyak dibutuhkan oleh masyarakat. Namun, berdasarkan data Badan Pusat Statistik menunjukkan bahwa produksi cabai mengalami penurunan yang salah satunya disebabkan oleh Colletotrichum capsici . Penelitian ini bertujuan untuk mengetahui pengaruh pemberian ekstrak daun sirih terhadap pertumbuhan C. capsici. Penelitian ini dilakukan menggunakan Rancangan Acak Lengkap (RAL) dengan 3 kali pengulangan. Proses ekstraksi daun sirih dilakukan dengan metode maserasi dengan pelarut etanol 96% dan uji antifungi dilakukan dengan metode difusi Kirby-Bauer. Konsentrasi ekstrak daun sirih yang digunakan adalah 5%, 10%, 15%, 20%, dan kontrol negatif menggunakan dimetil sulfoksida (DMSO). Data yang diperoleh dianalisis secara statistik menggunakan uji One-Way Anova dan uji lanjut Least Significant Difference (LSD). Hasil penelitian ini menunjukkan bahwa konsentrasi ekstrak daun sirih 20% menghasilkan zona hambat terbesar, yaitu 0,84 mm. Berdasarkan hasil pengujian disimpulkan bahwa ekstrak daun sirih memiliki aktivitas antifungi dalam menekan pertumbuhan C. capsici . Abstract Chili plants are important vegetables normally used by the community. However, based on the data of Badan Pusat Statistik , chili production is decreasing because of many factors, including Colletotrichum c apsici pathogen . This study was aimed to determine the effect of betel leaf extract on the growth of C. capsici. This research was conducted using a completely randomized design with 3 replications . The extraction process was carried out by the maceration method with 96% ethanol solvent and the antifungal test was performed by the Kirby-Bau e r diffusion method. The concentration of betel leaf extract used was 5%, 10%, 15%, 20% , and dimethyl sulfoxide ( DMSO ) was used as the negative control . The data obtained were analyzed statistically using the One-Way Anova test and the Least Significant Difference (LSD) posthoc test . The results of this study showed that the concentration of 20% betel leaf extract produced the largest inhibition zone of 0.84 mm. Based on the test results, it is concluded that betel leaf extract had antifungal activity in suppressing the growth of C. capsici.

This study was aimed to prepare polyeugenol with high molecular weight and to evaluate its antibacterial and antioxidant activities. First, polyeugenol was synthesized from eugenol in the presence of H2SO4-CH3COOH (4:1) as catalyst. The synthesized polyeugenol was weighed by using viscometer, revealing its high molecular weight of (7.76–21.9) × 105 g/mol. Furthermore, the antibacterial activity of the polyeugenol was conducted against Staphylococcus aureus and Escherichia coli bacteria. It was conducted by applying well diffusion method at 1, 2, 3, 4 and 5% concentrations to observe inhibition zones, in which the tests showed that the antibacterial activity of the polyeugenol against S. aureus were 17.42, 17.76, 18.79, 21.42 and 22.55 mm, while those against E. coli were 15.87, 17.23, 17.56, 18.24 and 19.21 mm, respectively. In short, these results indicated a strong antibacterial activity. Then, tests on antioxidant activity against free radical DPPH (2,2-diphenyl-1-pycrylhydrazyl) gave the IC50 value of 80.47 µg/mL, indicating a strong antioxidant activity. Therefore, the polymer synthesized in this work has a high potential to be applied in various biomedical applications.

Three stigmastane steroids: 6-hydroxystigmast-4-en-3-one (1), stigmast-4-en-3-one (2), and 3-hydroxystigmast-5-en-7-one (3) were successfully isolated from the acetone extract of Dryobalanps oblongifolia stem bark. The structural determination of isolated compounds was carried out on the basis of data analysis of NMR and MS spectra. In order to identify the antiplasmodial activity, the isolated compound was put to test against Plasmodium falciparum 3D7. Antiplasmodial activity of the isolated compounds showed that the IC50 values of 6-hydroxystigmast-4-en-3-one were 37.29 μg/mL while the IC50 values of stigmast-4-en-3-one were 43.54 μg/mL and the IC50 values of 3-hydroxystigmast-5-en-7-one were 13.34 μg/mL (chloroquine phosphate was used as a positive control, IC50 0.006 μg/mL). Judging from the results, the isolated compounds were proven to demonstrate mediocre antiplasmodial activity. Compound (3) indicated a better antimalarial activity than compound (1) and (2), even though there was no satisfactory activity that indicated its ability to combat chloroquine. Therefore, it might not be developed as an antimalarial drug.

Hyperglycemia, a primary symptom in diabetes mellitus, is associated with difficulties in wound healing and regeneration. This condition is due to the length of the inflammatory phase and free radi...

Introduction: Acute toxicity effects appear within a short time following the oral administration of either a single dose or repeated doses of toxin within 24 hours. Acute toxicity testing involves the administration of a range of doses across several groups of experimental animals with one dose administered per group, followed by the observation of toxic effects and mortality. Objectives: The purpose of this study was to determine the lethal dose 50 (LD50) and acute toxicity of an ethanol extract of Syzygium myrtifolium leaves in white mice. Methods: Exposed groups consisted of a negative control group (carboxymethylcellulose sodium) and four treatment groups (500, 1000, 2000, and 4000 mg/kg body weight (bw)). Mortality was observed for 14 days following oral administration. Results: The results demonstrated an LD50 of 1995 mg/kg bw, categorised as moderately toxic. Observed toxic effects included white lesions in the lungs, blackened liver, organ swelling, and fluid accumulation in the abdominal cavity and thorax.

Purwoceng ( Pimpinella pruatjan Molk) is an original Indonesian herb which is known to have an aphrodisiac effect. The active compounds in Purwoceng potentially have hepatotoxic and nephrotoxic effect s .This study was aimed to analyze the effect of subchronic administration of Purwoceng roots ethanol extract to Wistar Strain of Rattus norvegicus rats . The method of this subchronic toxicity study was an experimental post test only with control group design . Forty male Rattus norvegicus were randomly divided into four groups and get 28-days treatment. Group A as control received a quad e s t and 1% CMC ( carboxymethyl cellulose ) , group B, C, and D were given Purwoceng roots ethanol extract of 42, 84, and 168 mg/KgBW/day and 1% CMC. Parameters tested were the levels of u rea, creatinine, SGPT ( serum glutamic pyruvic transaminase ) , SGOT ( serum oxaloacetic pyruvic transaminase ) , kidney and hepar histo pato logy.The results showed a statistically significant for the liver histopathological in group B, creatinine, urea, and kidney his t opathology in group C. Our study concluded that s ubchronic administration of Purwoceng ( Pimpinella pruatjan Molk) roots ethanol extract could induce hepatotoxicity at the 42 mg/Kg BW /day dose level and nephrotoxicity at the 84 mg/KgBW/day dose level.

Soybeans are the third most important commodity after rice and corn. Soybeans are also a commodity rich in protein. Soy serves as a very important source of vegetable protein to improve people's nutrition because in addition to being safe for health is also relatively cheap compared to animal protein sources. Ultisol has a wide distribution but has constraints to be used as a medium of cultivation plants. The addition of some types of ameliorating or soil reformers such as chicken manure, cow manure, and composting oil palm bunches (OPB) is expected to overcome ultisol fertility. The purpose of this study was to compare the influence of varieties on the growth and component of soybean crop yields in Ultisol, compare the influence of ameliorating type on the growth and component of soybean crop yields in Ultisol, and get the interaction between the type of varieties with the type of amelioration to the growth and components of soybean crop yields in Ultisol. The research was conducted from February to May 2019 in the experimental garden of Integrated Agricultural Zone of the Faculty of Agriculture UNIB Medan Baru, Bengkulu City. This study used a Complete Randomized Design (CRD) that was compiled factorially with 3 times. The first factor is the variety type Anjasmoro and Gepak Kuning, the second factor of amelioration type is control (without amelioration), chicken manure 15 tons ha-1, cow manure 15 tons ha-1, husks burn 15 tons ha-1, and compost oil palm bunches (OPB) 15 tons ha-1. The results showed that the Gepak Kuning variety showed better growth than the Anjasmoro variety while the Anjasmoro variety showed better results than the Gepak Kuning variety. Chicken manure is the best type of amelioration compared to cow manure, rice husks, and compost OPB against the growth and component of soybean yields in Ultisol, and there is interaction in the Gepak Kuning variety with chicken manure amelioration and compost OPB.

to chemical contamination due to human activities. Chemical contaminants can come from heavy metals such as lead (Pb). Catfish can be used as a source of fish oil which may also be contaminated with lead metals. This study aims to determine the levels of lead (Pb) heavy metal contamination in the oil extract of catfish (Pangasius hypophthalmus). This research is an experimental laboratory research and the results are presented in descriptive form. The method used is the Atomic Absorption Spectrophotometry (AAS) method. The result of the determination of lead metal content in the catfish oil extract was 0 mg/kg.

Evaluation of compatibility on sweetpotato is very important to determine the crossing parents to increase the compatibility of controlled cross-pollination. This research was aimed to study the level of compatibility in controlled cross-pollination based on percentage of normal seedlings obtained from crosses. The research was conducted at Kendalpayak Experimental Station of ILETRI, Malang, Indonesia then followed by breaking seed dormancy and germination of seeds obtained. The genetic materials used were 17 accessions with high yielding potential and 3 accessions as tester for crossing. The results showed that there were differences in the compatibility levels of crossing combination and their compatibilities as male or female parents. Clone C-011 (Tester 2) was the most compatible as male parent, while clone C-141, C-007 and C-131 were the most compatible as female parents. Clone C-127 (Tester 3) was the most compatible as both male and female parents. Clone C-001 (Tester 1) could not be used as female parent due to high incompatibility.This compatible clone information is very useful in determining of both controlled crosses and open crosses in sweet potato in order to increase the breeding efficiency.

Efforts to improve the yield of kidney beans can be done through plant breeding programs, namely through introduction and selection activities. Selection is carried out to identify superior genotypes and will be effective if the relationship and influence between the characters are known. The research was conducted to study the closeness of the relationship between the characters of introduced kidney beans from Timor Leste and their direct and indirect effects through correlation and path analysis. This research was conducted at the Universitas Djuanda Experimental Garden, Ciawi, Bogor, in August-November 2020. The research design used was a randomized complete block design (RCBD) with accession as a factor and repeated 4 times. The results showed that the number of pods character had the highest correlation coefficient value on seed weight per plant as a yielding character for kidney beans with a value of r = 0.91 *. Besides, the number of pods also had a high direct (0.35) and indirect (0.91) effect through the characters of the number of seeds per pod (0.8) and the number of branches (0.3).

Bacillus thuringiensis is an entomopathogenic bacterium isolated from the soil and has been widely used as an active ingredient in the manufacture of bioinsecticides. The target insects are very specific and depend on the type of protein content. Epilachna sp. are important insect pests because both larvae and adults as plant-eating pests. The research aimed was to investigate the effectivity of B. thuringiensis against the larvae of Epilachna sp.. B. thuringiensis- bio-insecticide was prepared using isolates originally from freshwater swamp soil of South Sumatra (SMR04). Epilachna sp larvae were mass-reared with Solanum torvum leaf feed in the laboratory. The design used was a completely randomized design, with 6 treatments and 5 replications. Treatments were spore concentration contained in the bioinsecticide solution included: 1 x 108, 1 x 107, 1 x 106, 1 x 105 spores/mL, commercial bio-insecticide and without treatment as a control. Each replication used 10 individual of the 2nd larvae. Statistical test results showed larval mortality in B. thuringiensis treatment was significantly different from commercial bio-insecticide treatment. The highest mortality of bio-insecticide treatment occurred at a concentration of 108 spores/ml (40.00 %) and the lowest was at a concentration of 105 spores/mL (18.01 %). The lowest LT50 value of bio-insecticide treatment was at a concentration of 108 spores/mL, namely 79.37 hours. The control of larvae included in the Coleoptera order was still not satisfactory yet, considering the presence of protein content in B. thuringiensis strain SMR04 which did not match the type of protein required.

Impact of hexane defatting on physicochemical, oxidative stability and total volatile compounds of Bombay locust (Patanga succincta L.) powder ‘BL’ were studied. Defatted insect powder (F/BL) showed marked decrease in fat content (1.2%) with significantly (P≤0.05) higher protein content (78.7%), compared with those of BL (29.8% fat and 56.5% protein). Based on electrophoresis result, hexane defatting had no effect on peptide distribution of insect powders. F/BL had lower peroxide value with better oxidative stability (P≤0.05), while less variety of volatiles with more off-flavour compounds, particularly lipid oxidation and insect hormone odours, were observed from BL. F/BL was confirmed as an excellent source of amino acids and minerals. The physicochemical and sensory properties of F/BL fortified biscuit stick and the final products were determined. F/BL fortified biscuit stick showed a better acceptance, compared with those from BL at the same level used. Based on sensory characteristics, biscuit stick with 10% F/BL (F/BL-10) was selected and studied for its chemical composition and microstructure. F/BL-10 possessed more porous structure with higher protein and lower energy value, compared with those from 10% BL. Overall, defatted Bombay locusts could be an effective alternative protein source with excellent applicability to baked products, particularly biscuit stick.

Dry matter content is one of the important characteristics of sweet potatoes in addition to high yield. The aim of this study was to evaluate the performance of promising sweet potato clones for higher root yield and dry matter content. A hundred promising sweet potato clones were evaluated and arranged in randomized block design with two replication. The variables observed included: weight of vine, harvest index, number of root per plot, the weight of root per plot, dry matter content, root yield, root skin color, and flesh color. The analysis of variance showed a significant difference among the tested genotypes in all traits observed. A hundred promising sweet potato clones showed that root yield has varied ranged from 4.88–41.38 t h-1 with an average 20.28 t h-1 and dry matter content ranged from 19.19 – 40.65% with an average 30.47%. Fifteen promising clones of a hundred clones tested had high root yield and dry matter content with varying color flesh.

This naturalistic qualitative study is intended to unravelling the working of quantity maxim introduced by Grice (1975) on EFL online classroom interaction amid the Covid-19 pandemic. Besides, this study is designed to investigate whether or not students violating or obeying the quantity maxim by observing and unravelling the utterances produced during online classroom interaction. Moreover, this study also provides factors that influence the non-observance maxim of quantity. The participant consists of 31 students from the fifth semester majoring in English Education at Tidar University. The study is conducted by recording, transcribing, and classifying the conversations done on online classroom interaction, viewed from Gricean maxim perspectives. The results reflect that students both observed and violated the maxim of quantity. The total number of observance maxim is 9, and 14 for the non-observance maxim. This study brings the conclusion that external factors such as unstable internet connection and interference coming from the surrounding environment during the online classroom can cause EFL students to break the maxim of quantity principle. Meanwhile, anxiety as an internal factor plays a crucial factor that leads the EFL students to violate the quantity maxim.

Research on the extraction and stability test of natural dye from red spinach (Alternanthera amoena Voss) has been carried out. This study aims to determine the effect of pH and length of sun exposure on the stability of the dye from red spinach. Extraction using the maceration method with ethanol solvent and measurement using a UV-Vis spectrophotometer. Stability testing is carried out at pH 2-5 and sun exposure for 1-4 hours using a dark and light container. The results obtained, at pH 2 and pH 3, anthocyanins were more stable, compared to pH 4 and 5 which had greater color degradation. In exposure to sunlight, dark containers with a degradation percentage of 31.70% were better than clear containers with a percentage of 48.78%. Keywords: Anthocyanin, red spinach, natural dyes stability.

The concern of oil palm empty fruit bunches (OPEFB) utilization in the rubber field that potential to be a host of white root disease needs to be deeply investigated. The objective of this study was to assess the bacteria on the surface of OPEFB that able to inhibit the growth of R. microporus fungi. The antagonistic assay of eight isolates of bacteria against R. microporus fungi was conducted in the Plant Protection Laboratory of Sungei Putih Research Centre. The isolates were isolated from the surface of OPEFB applied in the rubber field. Nutrient agar (NA) was employed in the isolation and purification of bacteria. The antagonistic assay was carried out in vitro using the dual culture method. The observation was performed on 2, 4, 6, and 8 days after incubation (DAI). The result indicated that the eight isolates have varied in inhibition ability of R. Microporus fungi. The initial inhibition was started since 2 DAI particularly in the isolates B2, B3, B4, and B7. The percentage of inhibition were 30%, 60%, 65% dan 69% respectively. Until 8 HSI, the isolates B2, B3, B4, B6, B7, and B8 were still inhibiting the growth of R. microporus fungi > 80%. The isolates B3 and B4 showed the best performance in fungi inhibition with the percentage > 95%. Whereas, the ability of inhibition of the isolates B1 and B5 were very low as < 50%. The existence of such antagonistic bacteria perhaps led to OPEB was not role as the host of white root disease while applied in the rubber field

Public awareness of foods that have physiological functions for health is increasing. Consumption of orange fleshed sweetpotato not only meets carbohydrate requirements but also beta-carotene which is good for eye health. The aim of this reserach was to evaluate the yield potential and levels of dry matter content of promising clones of orange fleshed sweetpotato rich in beta-carotene. The study was conducted in Malang, East Java in Dry Season II 2016. Genetic materials used were 20 sweetpotato clones, including Beta-1 and Beta-2 varieties as a check. The treatment design used a randomized block design with three replications. The results showed that the clones tested had a potential yield ranging from 20.9 - 35.1 t/ha with an average of 25.2 t/ha. There were three clones had a higher yield potential than the check varieties Beta-1 and Beta-2. Dry matter content ranged from 17.0 - 31.6% with an average of 25.7%. Fifteen clones had higher levels of dry matter content than check varieties Beta-1 and Beta-2. MSU 14018-06, MSU 14001-20 and MSU 14027-02 clones had high yield potential and dry matter content. All three clones have the opportunity to be released as new superior varieties of orange fleshed sweet potato rich in beta-carotene.

Tomatoes (Lycopersicon esculentum Mill.) are a source of carotenoids they are easy to find. These compounds function as precursors of vitamin A, antioxidant, and prevent cancer. The extraction of carotenoid compounds for commercial products usually uses fresh plant s, which are less efficient and require a lot of raw materials. The supply of these raw materials can be done through tissue culture. The frequency of subculture or supply of nutrients in tissue culture is very influential on the content of callus carotenoids produced. This study aim ed to determine the effect of subculture frequency on growth, development and callus carotenoid content and to find out the right frequency of subculture to produce callus with optimal growth, development and carotenoid content. The design this study was a single Completely Randomized Design (CRD) with 4 treatments of subculture frequency and 5 replications. The data obtained were analyzed by ANOVA at the 95% test level followed by DMRT in case a significant different was found The results showed subculture frequency affected growth, development and carotenoid content of callus Lycopersicon esculentum. Mill. The most optimal treatment to induce growth and production of carotenoids in this study was treatment of thrice subculture while the most optimal treatment in inducing development was the twice subculture treatment .

In the millennial era unhealthy eating patterns and lifestyles associated with excess weight gain (obesity), diabetes mellitus, cholesterol and pregnancy complications, concomitant diseases such as heart disease, cholesterol, mothers exposed to alcohol, cigarettes or narcotics must be know. This study aimed to know the effectiveness of media books for pregnancy detection. The keywords used were detection of pregnancy risk, symptoms and signs of pregnancy. This literature review used articles from Google Scholar, Science Direct, Pubmed, DOAJ published from 2010 to 2020. The inclusion criteria are full text articles, using English language, survey research, workshop, and pilot study to health promotion. The exclusion criteria are articles was non English language, incomplete articles, case study. Containing symptoms and danger signs is effective for risk detection in early pregnancy

Microbial enhanced oil recovery (MEOR) is a tertiary oil recovery process which aims to increase the crude oil recovery uptake to 55% from its original oil in place. In its application, indigenous microorganisms are commonly used as the main biological agents, but the isolation processes become more complicated due to environment’s extreme condition. This study aims to determine the optimum formulation for the enrichment and isolation medium, in order to obtain a highly diverse of indigenous hydrocarbonoclastic bacteria. The isolation process was carried out in two stages; the isolation of mild oil fraction degrading bacteria, and isolation of the heavy oil fraction degrading bacteria which confirmed by the SARA (saturated, aromatic, resinic, and asphaltic) fraction chromatography analysis. Four enrichment and five isolation media were formulated based on the distinction in the macro-and micronutrient composition. Overall, a total of 92 isolates were successfully isolated. The highest bacterial diversities were achieved in the enrichment medium B (crude oil used as carbon source which supplemented with sodium lactate) and isolation medium I (nutrient agar with twice agar concentration), each with 37 and 33% of obtained isolates, respectively. The newly formulated medium can be further utilized to isolate various types of indigenous bacteria from the oil well which were able to degrade SARA fraction from crude oil. The result showed that ABG2-7 as the isolate with the highest activity on SARA degradation. The amount of 15% inoculum of ABG2-7 isolate was able to degrade heavy crude oil, thus might serve as potential bacteria for MEOR application.

Germplasm as a source of genes in sweetpotato breeding requires information on appearance and genetic parameters. The objectives of this research were to determine the performance and genetic parameters of sweet potato accessions. The research was conducted at Kendalpayak Research Station, Malang, East Java, Indonesia. The materials used were thirty sweet potato accessions from Indonesian Legumes and Tuber Crops Research Institute (ILETRI) germplasm collection. The research was arranged in a Randomized Block Design (RBD) with two replications. The variables observed included: the vines length, the weight of vines, the number and weight of the saleable root per plot, the number and weight of the non-saleable root per plot, the number and weight of root per plant, the root yield, the harvest index, and the dry matter content. The results of ANOVA showed a significant difference among the tested genotypes in almost all traits observed except on weight of non-saleable root. PCV estimation was higher than GCV estimation for all the observed characters. The weight of the saleable root per plot, the weight of root per plant, and the root yield that showed a wide range of PCV and GCV as well as high broad-sense heritability indicated that these traits had additive gene effect and more reliable for effective selection. The broad GCV in a population is effective for selection to obtain the superior variety.

Ambonese arrack is one of the traditional fermented beverage product in Maluku, Indonesia. The microbiological research of this beverage that is processed using coconut sap as raw material has never been done before. The research aimed to analyze the microbial succession and chemical characteristics during fermentation of Ambonese arrack. The sample of coconut sap was taken from traditional producer in Mahia village, Ambon. The dominant microbes in the fermentation of Ambonese arrack were Pichia polymorpha and Kloeckera javanica. The highest numbers of these two microbes was obtained after 15 hours fermentation (9.6 log Cfu/mL and 9.9 Cfu/mL, respectively). The sugar content decreased from 593.3 mg/L to 474.3 mg/L, whereas ethanol content increased from 0.0018 g/L to 0.0100 g/L. The pH value decreased from 4.70 to 3.10. The research has isolated indigenous microbes in Ambonese arrack fermentation which was considered as novelty. The bacteria that play a role in fermentation can be used as a starter in the fermentation of various beverage products, especially Ambonese arrack. The results of this research can improve the quality of this fermentation product in the future.

Penicillium marneffei is a thermally dimorphic fungus that causes penicilliosis, and become the third-most-common opportunistic fungal infection in immunocompromised patients in Southeast Asia. Azoles and amphotericin B have been introduced for the treatment, however, it is important to investigate possible mechanisms of azole resistance for future treatment failure. We identified 177 putative MFS transporters and classified into 17 subfamilies. Among those, members of the Drug:H+ antiporter 1 subfamily are known to confer resistance to antifungals. Out of 39 paralogs, three (encoded by PmMDR1, PmMDR2, and PmMDR3) were heterologously overexpressed in S. cerevisiae AD∆ conferred resistance to various drugs and compounds including azoles, albeit to different degrees. PmMDR1-expressing strain showed resistance to the broadest range of drugs, followed by the PmMDR3, and PmMDR2 conferred weak resistance to a limited range of drugs. We conclude that PmMDR1 and PmMDR3, may be able to serve as multidrug efflux pumps.

Cymbopogon citratus (lemongrass) has a great potential to be commercialized as an anti-diabetic medication due to its alpha glucosidase inhibitor (AGI) activity. To achieve this goal, this paper continues the study of extraction optimization at the pilot scale to determine the effect of sample quantities on AGI activity. This experiment comprised three phases: designing the percolator, optimizing the process parameters and conditions, and determining the correlation between various sample quantities and AGI activity. The effects of macerating the plant material prior to percolation and using different solvent flow rates during extraction were observed. Four different variations were used in the extraction process trials: 63.09cm3 s-1, 94.64 cm3 s-1, 126.20 cm3 s-1, and 189.30 cm3 s-1. Sample quantities of 400 g and 500 g were used to upscale the analysis. The results showed that maceration did not significantly increase AGI activity (P?0.17), but it did shorten the time needed to reach equilibrium concentration. Similarly, the solvent flow rate variations did not affect AGI activity (P?

Isolation and identification of secondary metabolite of ethyl acetate extract of jackfruit stem (Artocarpus heterophyllus Lmk.) as well as its activity as antibacterial have been carried out. The purpose of this study was to know the characterization and antibacterial activity of secondary metabolites from ethyl acetate extract of the Jackfruit stem. Ethyl acetate extract was fractionated and purified using several chromatographic techniques. The identification of the isolated compound was elucidated by spectroscopic UV-Vis, IR, and NMR. Based on data analysis of UV, IR, and NMR spectra showed that the isolated compound was a flavonoid derivative. Antibacterial activity test of the isolated compound to Escherichia coli and Staphylococcus aureus was done by a well diffusion method. The inhibitory zone values of flavonoid compound in concentration series of 1000 µg/ml, 3000 µg/ml, and 5000 µg/ml against E. coli were 9.65 mm, 10.25 mm, and 11.00 mm, respectively. While, the inhibitory zone values in the same concentration series against S. aureus were 9.00 mm, 10.75 mm, and 11.38 mm. Keywords: Jackfruit, Artocarpus heterophyllus Lmk., flavonoid, antibacterial activity.

Background: White turmeric (Curcuma zedoaria), a plant used traditionally for medicine, is easily obtained at a relatively cheap price in Indonesia. White tumeric contains alkaloids, phenols, saponins, glycosides, steroids, terpenoids, and other compounds; and these compounds have shown antimicrobials, antifungal, anticancer, antiallergic, antioxidant, and analgesic effects. The aim of this study was to determine the antibacterial effect of white turmeric (Curcuma zedoaria) rhizome extract on the growth of Bacillus cereus and Staphylococcus epidermidis bacteria. Methods: This was an experimental study with a Post-test Only Control Group Design. It was carried out using the disc diffusion method with six treatments, consisting of negative control (aquadest), positive control (ciprofloxacin), and the extract of white turmeric rhizome with various concentrations. Kruskal Wallis test and one way ANOVA were used to analyze the data. The results showed a statistically significant value smaller than α (0.021 20mm. Conclusions: Curcuma zedoaria has an antibacterial effect against Bacillus cereus and Staphylococcus epidermidis. Further study is needed, exploring the effectivity of white turmeric in the animal models.

This study aims to develop students' interest and creativity in opening business opportunities that can increase experience, motivate them to develop innovations and creations. One of them is by using rattan. Rattan is a group of palms from the tribe (tribus) Calameae which has a climbing habitus, especially Calamus, Daemonorops, and Oncocalamus. Rattan grows wild in the forest or is planted intentionally. Rattan can be harvested at any time, with the attention that the bottom of the stem is not covered by petals, the leaves have dried, the thorns and petals have fallen off. Masaih rattan is easy to find in our area. Therefore we have the initiative to make handicrafts or items that are useful and value for sale. So, we STKIP Singkawang students innovate to make a study of rattan. In addition, to add to the attractiveness and selling value, we also add various forms so that buyers are more interested and for the price problem offered also according to the shape and size of the goods being produced. To market this product we sell it directly and through social media.

A study was conducted on the antibacterial activity test on n-hexane, ethyl acetate, and ethanol extracts of ebony bark (Diospyros celebica Bakh.) using a diffusion method. The purpose of this research is to know the antibacterial activity of ebony bark against Staphylococcus aureus and Escherichia coli bacteria using n-hexane, ethyl acetate dan ethanol. The method of extraction used in this research was maceration method with three polarity levels of solvent. The antibacterial activity test was performed by diffusion-well test. The results showed that ethyl acetate extract gave the highest inhibition of 26.14 mm in gram-positive bacteria (Staphylococcus aureus) and ethanol extract of 25.97 mm in gram-negative bacteria (Escherichia coli). Keywords: Ebony (Diospyros celebica. Bakh), Tree Bark, Inhibitory, Antibacterial

The purposes of this research the examine the effectiveness various concentrations neem leaf ekstract on the intensity of attack Liriomyza spp on shallot Lembah Palu variety . Field research was carried out in Guntarano Village, Tanantovea Subdistrict, Donggala Regency, Central Sulawesi. This research conducted from February to April 2016 .The experiment was done using Randomized Complete Block Design with three replications, consists of six treatments, i.e. P0 : control (without aplication neem leaf ekstract); P1: neem leaf ekstract 10 gr /litre; P2 : neem leaf ekstract 20 gr / litre; P3 : neem leaf ekstract 30 gr / litre; P4 : neem leaf ekstract 40 gr / litre; P5 : neem leaf ekstract 50 gr / litre. To find out the effect of threatment, the analysis of variance, and the real influence is continued with the standard ά = 0.05 BNT test. The results showed that use of seed and leaf extracts of neem plant can control Liriomyza spp attack, only 3,04 percent of attack rate and shallot Lembah Palu variety productivity is 3,02/area.

Palembang fish crackers have a thick characteristic with a strong fishy aroma. Puffing of the thick fish cracker requires particular technique, for example the deep-frying techniques should use two different heating temperatures to obtain good quality of crackers. Another alternative method is to use heat from microwaves. One of the disadvantages of crackers that are processed with heat from the microwave is the easiness of the crackers lose its crispness. This study aimed to analyze the shelf-life of fish crackers puffed by microwave heat and deep-frying technique in some types of packaging. The research design was a factorial completely randomized design with two factors namely the type of packaging (polypropylene, nylon, metalized plastic) and type of crackers (microwaved and fried). Analysis of variance showed that all treatments have a significant effect on water content. The indicator used for the prediction of shelf-life is water content. The water content changes during storage at room temperature for all treatments followed a first-order kinetic with the lowest k value for fish cracker packed in metalized plastic packaging with 57 days storage time (microwavable fish crackers) and 45 days (fried fish crackers)

Kidney stones disease is asignificant health problem in the world and Indonesia is no exception. Traditionally, avocado seeds can be used as a laxative medicine for kidney stones disease. Elixiris a clear and sweet hidroalkohol solution intended for oral use and a flavor is usually added to enhance the taste. This study was to determine theformula of the avocado seed elixir which has the best quality and the level of flavonoids.Elixir testing was performedusing several methods such as organoleptic, limpidity, viscosity, pH and specific weightas well as total flavonoids. The result showed that the best elixir formula was the Formula I which composed of 7%avocado seed extract, 5% ethanol, 10% propylene glycol, 40% sirupus simplex, 0.1%, essence mint and 100%aquadestilata ad. Furthermore, Elixirhad a brown color, mint aroma and pretty sweettaste. The test results showed that elixir had a viscosity of 7.92 cP; pH of 5.65; specific weight of 1.0413 g/mL andtotal flavonoids of 2.1020%.

Dengue exhibits focal clustering in households and neighborhoods, driven by local mosquito population dynamics, human population immunity, and fine scale human and mosquito movement. We tested the hypothesis that spatiotemporal clustering of homotypic dengue cases is disrupted by introduction of the arbovirus-blocking bacterium Wolbachia (wMel-strain) into the Aedes aegypti mosquito population. We analysed 318 serotyped and geolocated dengue cases (and 5,921 test-negative controls) from a randomized controlled trial in Yogyakarta, Indonesia of wMel deployments. We find evidence of spatial clustering up to 300m among the 265 dengue cases (3,083 controls) in the untreated trial arm. Participant pairs enrolled within 30 days and 50m had a 4.7-fold increase (compared to 95% CI on permutation-based null distribution: 0.1, 1.2) in the odds of being homotypic (i.e. potentially transmission-related) as compared to pairs occurring at any distance. In contrast, we find no evidence of spatiotemporal clustering among the 53 dengue cases (2,838 controls) resident in the wMel-treated arm. Introgression of wMel Wolbachia into Aedes aegypti mosquito populations interrupts focal dengue virus transmission leading to reduced case incidence; the true intervention effect may be greater than the 77% efficacy measured in the primary analysis of the Yogyakarta trial.

Although propolis has been reported for having anti-inflammatory activities, its effects on complement system has not been much studied. This research was conducted to find out the effects of Indonesian propolis on the expression levels of C3, C1r/s, Bf, MBL, and C6 in zebrafish larvae which were induced by lipopolysaccharide (LPS). Counting of macrophages migrating to yolk sac and liver histology were carried out. Larvae were divided into four groups: CON (cultured in E3 medium only), LPS (cultured in a medium containing 0.5 μg/L LPS), LPSIBU (cultured in a medium containing LPS, and then treated with 100 μg/L ibuprofen for 24 hours), and LPSPRO (cultured in a medium containing LPS, and then immersed in 14,000 μg/L propolis for 24 hours) groups. The results showed that complement gene expression in larvae from the LPSIBU and LPSPRO groups were generally lower than in larvae from the LPS group. The number of macrophage migrations to the yolk in the LPSPRO group was also lower than in the LPS group. Histological structure of liver in all groups were considered normal. This study shows that Indonesian propolis has the potential to be used as an alternative to the substitution of NSAIDs. Resumo Embora a própolis tenha sido relatada por ter atividade anti-inflamatória, seus efeitos no sistema complemento, uma parte do sistema imunológico inato, não foram muito estudados. Esta pesquisa foi conduzida para descobrir os efeitos da própolis da Indonésia nos níveis de expressão de C3, C1r/s, Bf, MBL e C6 em larvas de peixe-zebra induzidas por lipopolissacarídeo (LPS). Foram realizadas contagens de macrófagos que migram para o saco vitelino e histologia do fígado. As larvas foram divididas em quatro grupos: CON (cultivadas apenas em meio E3), LPS (cultivadas em meio contendo 0,5 μg/L de LPS), LPSIBU (cultivadas em meio contendo LPS e, em seguida, tratadas com 100 μg/L de ibuprofeno por 24 horas) e LPSPRO (cultivado em meio contendo LPS, e então imerso em própolis 14,000 μg/L por 24 horas). Os resultados mostraram que a expressão do gene do complemento em larvas dos grupos LPSIBU e LPSPRO foi geralmente menor que em larvas do grupo LPS. O número de migrações de macrófagos para a gema no grupo LPSPRO também foi menor que no grupo LPS. A estrutura histológica do fígado em todos os grupos foi considerada normal. Este estudo mostra que a própolis indonésia tem potencial para ser utilizada como alternativa na substituição dos AINEs (anti-inflamatórios não esteroides).

BACKGROUND: Aedes aegypti mosquitoes infected with the wMel strain of Wolbachia pipientis are less susceptible than wild-type A. aegypti to dengue virus infection. METHODS: We conducted a cluster-randomized trial involving releases of wMel-infected A. aegypti mosquitoes for the control of dengue in Yogyakarta, Indonesia. We randomly assigned 12 geographic clusters to receive deployments of wMel-infected A. aegypti (intervention clusters) and 12 clusters to receive no deployments (control clusters). All clusters practiced local mosquito-control measures as usual. A test-negative design was used to assess the efficacy of the intervention. Patients with acute undifferentiated fever who presented to local primary care clinics and were 3 to 45 years of age were recruited. Laboratory testing was used to identify participants who had virologically confirmed dengue (VCD) and those who were test-negative controls. The primary end point was symptomatic VCD of any severity caused by any dengue virus serotype. RESULTS: After successful introgression of wMel into the intervention clusters, 8144 participants were enrolled; 3721 lived in intervention clusters, and 4423 lived in control clusters. In the intention-to-treat analysis, VCD occurred in 67 of 2905 participants (2.3%) in the intervention clusters and in 318 of 3401 (9.4%) in the control clusters (aggregate odds ratio for VCD, 0.23; 95% confidence interval [CI], 0.15 to 0.35; P = 0.004). The protective efficacy of the intervention was 77.1% (95% CI, 65.3 to 84.9) and was similar against the four dengue virus serotypes. The incidence of hospitalization for VCD was lower among participants who lived in intervention clusters (13 of 2905 participants [0.4%]) than among those who lived in control clusters (102 of 3401 [3.0%]) (protective efficacy, 86.2%; 95% CI, 66.2 to 94.3). CONCLUSIONS: Introgression of wMel into A. aegypti populations was effective in reducing the incidence of symptomatic dengue and resulted in fewer hospitalizations for dengue among the participants. (Funded by the Tahija Foundation and others; AWED ClinicalTrials.gov number, NCT03055585; Indonesia Registry number, INA-A7OB6TW.).

Health promotion activities about first aid for syncope cases of the Senior High School of Antartika, Buduran, Sidoarjo is one form of community service in the form of counseling aimed at increasing children's knowledge about how first aid in syncope besides fatigue, syncope can also be caused by overheating, being trapped in space closed to lack of oxygen, shocked or surprised. The implementation of these activities on November 20, 2019 took place at the Senior High School of Antartika, Buduran, Sidoarjo. The target is Senior High School students of Antartika, Buduran, Sidoarjo. Before the activity is carried out, there is a process of compiling the activity for approximately 2 weeks before the activity is carried out, starting from determining the theme of counseling to submitting licensing to the parties concerned. As a form of evaluation, the activity was attended by 30 class X students, participants took part in the activity happily and looked very enthusiastic, health promotion activities could run on time and smoothly. Keywords: Knowledge, syncope cases, first aid health promotion.

Serratia sp is the family Enterobacteriaceae gram-negative bacteria that have flagella Peritrik, motile and reported to be anti-fungal. Chitinolytic chitinase activity of bacteria are potentially used as a biological control against pathogenic fungi.The purpose of this research is to know the potential of bacteriaSerratia sp in controlling white root rot disease caused by Rigidoporus microporus on the rubber plant in polibag. This research uses a randomizedblock design (RAK) nonfaktorial with 5 treatments and 5 replications. Parametersmeasured were extensive white root fungus colony growth (cm) in the laboratory,white root fungus attack intensity (%) in polybag, the percentage of inhibition (%)in polybag and visual symptom of the white root fungus attack in polybag. Thereault of this showed that Serratia sp bacteri application infection very obvious inobstructing extensive growth of the white root fungus colony in Laboratory, withthe lowest extensive of colony 3,06 cm on D4 standard (10ml/100 ml PDA) A verysignificant effect in reducing the intensity of white root fungus attacks the inhibition rate reached 54%, or healing reached 47,31% the age of 6 weeks after application.

This study was aimed at determining the concentration of several types of cytokinins and auxin for the induction of turmeric shoots in vitro. The research was conducted at the Tissue Culture Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University, Jatinangor. The study was conducted from October 2017 to February 2018. The source of planting material is in the form of shoots from the turmeric rhizome. The source of explants or planting material came from the field collected at the Tissue Culture Seed Technology Laboratory, Faculty of Agriculture, Padjadjaran University. Explants were taken from rhizome buds with a size of 0.6-2.0 cm. The experiment used a Completely Randomized Design which was analyzed using the Student’s T-test method. The number of experimental and control groups in this study were seven groups. Variation in treatment with different BAP, thidiazuron, zeatin, and NAA concentrations in each group. The results show that Thidiazuron 1 mgL-1 + NAA 1 mgL-1 gives better results on the percentage of live explants and number of shoots on turmeric plants (Curcuma domestica Val.) Clones 41 at the age of 14 weeks after planting.RESPONS EKSPLAN KUNYIT PADA SITOKININ DAN AUKSIN DALAM MEDIA MURASHIGE DAN SKOOGPenelitian ini bertujuan untuk mendapatkan salah satu konsentrasi dari beberapa jenis sitokinin dan auksin untuk induksi tunas kunyit secara in vitro. Penelitian dilakukan di Laboratorium Kultur Jaringan Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran, Jatinangor. Waktu pelaksanaan penelitian ini dimulai pada awal bulan Oktober 2017 sampai bulan Februari 2018. Sumber bahan tanam berupa tunas dari rimpang tanaman kunyit. Sumber eksplan atau bahan tanam berasal dari lapangan yang dikoleksi di Laboratorium Kultur Jaringan Teknologi Benih, Fakultas Pertanian, Universitas Padjadjaran. Eksplan diambil dari mata tunas rimpang dengan ukuran 0,6-2,0 cm. Percobaan menggunakan Rancangan Acak Lengkap yang dianalisis menggunakan metode Student’s T-test. Jumlah kelompok eksperimen dan kontrol dalam penelitian ini adalah tujuh kelompok. Variasi perlakuan dengan penambahan konsentrasi BAP, thidiazuron, zeatin, dan NAA yang berbeda pada setiap kelompok. Hasil penelitian ini menunjukkan bahwa Thidiazuron 1 mgL-1 + NAA 1 mgL-1 memberikan hasil yang lebih baik pada persentase eksplan hidup dan jumlah tunas pada tanaman kunyit (Curcuma domestica Val.) klon 41 pada umur 14 MST (Minggu Setelah Tanam).

Indriani F, Siregar U, Matra DD, Siregar IZ. 2019. Ecological aspects and genetic diversity of Shorea balangeran in two forest types of Muara Kendawang Nature Reserve, West Kalimantan, Indonesia. Biodiversitas 20: 482-488. Shorea balangeran (balangeran) belongs to the Dipterocarpaceae family that is distributed naturally in Indonesia with a wide range of ecological conditions including peat swamp and heath forests. Good adaptability of S. balangeran remains unknown concerning molecular (genetic) responses that are important to provide scientific knowledge for supporting forest restoration, especially for peatland restoration efforts. The main objective of this research was to analyze the ecological aspects and genetic diversity of S. balangeran in two types of forests, namely: peat forests (wetland) and heath forests (dry land). Field research was conducted in Muara Kendawangan Nature Reserve, West Kalimantan following standard vegetation analysis by establishing purposively sample plots (50 m x 50 m). Genetic diversity was assessed by RAPD analysis using ten random polymorphic primers. Results showed that both in heath and peat forests, S. balangeran was dominant, but only at pole and tree stages indicating that poor regeneration capacity of S. balangeran. This finding also confirmed the critically endangered status of S. balangeran as listed in the IUCN Red List of Threatened Species. Genetic analysis using RAPD did not show a clustering of S. balangeran between peat forest and heath forest populations, indicating that S. balangeran has good adaptability to grow in both forest types.

Eichhornia crassipes is well-known as water hyacinth. Water hyacinth grows rapidly in the nutrient-rich water and high light intensity places. The uncontrollable growth of water hyacinth has caused many negative impacts to the environment. For instance, interrupted water transport and decreased population of aquatic lives. The capacity of utilising water hyacinth is slower than water hyacinth growth and water hyacinth is still considered as a threat to theecosystem. This work was focused on the study of the pharmacological activity and heavy metal content of water hyacinth in Lake Cipondoh, Tangerang. Fresh water hyacinth was pre-treated through oven-drying and milling process. After that, each part of the plant was macerated by using multiple extraction method with 96% ethanol/water and three variations of sample-to-solvent ratios (1:30, 1:50, and 1:75 w/v). The result of the experiment showed thatwater hyacinth leaves produced an extract with lowest IC 50 (55.76 ± 6.73 ppm) compared toother parts. The most optimum solvent used to achieve this result was 96% ethanol/water (1:1 v/v). In order to obtain the lowest antioxidant activity, the sample to solvent ratio used was 1:50 and the heavy metal in the extract was very low. With this result, it was concluded that there is a promising opportunity to apply the water hyacinth growing in Lake Cipondoh, Tangerang as herbal medicine ingredient. Through this utilization, the overall number of water hyacinth in Indonesia can be reduced or at the least be controlled, so that the environmental problem caused by this plant can be minimized.

Cymbopogon citratus and Cymbopogon nardus are two species of Cymbopogon that are commonly cultivated in Indonesia. Studies have shown Cymbopogon activities and potencies for development for various diseases including diabetic treatment. Both C. citratus and C. nardus have long history for culinary and medicinal herb in Indonesia and therefore have a sustainable commercial production that will support their development for functional food ingredient. This work provides a study about industrial observation in several herbal industries along with literature review of herbal industries condition in Indonesia as a benchmarking study to summarize the required research or technical development of Cymbopogon as functional food ingredients. During industrial observation it was shown that Cymbopogon usage in herbal industries were more popular as flavorings rather than for its functionalities. The industrialobservation to several reputable herbal industries showed that despite the similar infrastructure used for extraction and pulverization method, the industries might have different supply procurement and quality requirement, extraction principle, product character and quality control, and therefore market character. The literature review have shown several studies of Cymbopogon functionalities in organic solvents, empirical uses of Cymbopogon as medicinalherbs in water extract, optimization in Cymbopogon cultivation. Heavy studies have been done in the extraction, usage, and characterization of essential oils from Cymbopogon. Many literature showed formulation of Cymbogon for culinary purpose but there are yet formulation based on its functional activities. Both industrial observation and literature review called in the requirement of more study of activities and stabilities of the Cymbopogon aqueous andethanolic extracts, optimization of post-harvest processing, extraction, and further processing to produce Cymbopogon ingredients based on activities and efficiency, and the determination of active fraction/compound(s) responsible for the activity and formulation of food that will enable the usage direction of functional food ingredients.

Biosurfactants are one of the microbial bioproducts that are in most demand from microbial-enhanced oil recovery (MEOR). We isolated and screened potential biosurfactant-producing bacteria, followed by biosurfactant production and characterization, and a simulation of the MEOR application to biosurfactants in a sand-packed column. Isolate screening was conducted based on qualitative (hemolytic blood assay and oil-spreading test) and semi-qualitative (emulsification assay and interfacial tension measurement) parameters. Bacterial identification was performed using 16S rRNA phylogenetic analysis. Sequential isolation yielded 32 bacterial isolates, where Pseudomonas sp. G3 was able to produce the most biosurfactant. Pseudomonas sp. G3 had the highest emulsification activity (Ei = 72.90%) in light crude oil and could reduce the interfacial tension between oil and water from 12.6 to 9.7 dyne/cm with an effective critical-micelle concentration of 0.73 g/L. The Fourier transform infrared spectrum revealed that the biosurfactant produced was a glycolipid compound. A stable emulsion of crude extract and biosurfactant formed at pH 2–12, up to 100 °C, and with a NaCl concentration of up to 10% (w/v) in the response-surface method, based on the Box–Behnken design model. The sand-packed column experiment with biosurfactant resulted in 20% additional oil recovery. Therefore, this bacterium and its biosurfactant show potential and the bacterium is suitable for use in MEOR applications.

Cabbage (Brasicca oleracea L.) is one type of vegetable plant from the Brassicaceae family. This plant is a source of nutrients that contain nutrients, vitamins, minerals and fiber for the human body. And has economic value. Cabbage cultivation is influenced by the types of cultivated varieties that are in accordance with growing conditions. In general, cabbage plants grow on highlands. Along with advances in plant breeding technology, cabbage plants are available that can adapt to the lowlands and technically attempt to increase production by fertilizing. The study aimed to determine the effect of beef biourine, red onion extract and beef biourine and red onion extract on the agronomic properties of cabbage plants. The study was conducted for 4 months, from February to June 2018. The location of the study was carried out in the Sempaja Timur Village, Samarinda City, East Kalimantan Province. cabbage seeds, top soil, manure, husks, NPK fertilizer, cow urine, empon-empon, soybeans, brown sugar, shallots. The design used in this study is Factorial Randomized Block Design (RBD) with 2 treatments. The first treatment was beef biourine (S) with 4 levels, namely with 3 replications: s0: control (water), s1: 25% concentration of cow biourine, s2: 75% concentration of bovine biourine, s3: 100% concentration of bovine biourine and second treatment red onion extract (B) with 4 levels, namely: b0: control (water), b1: 25% concentration of red onion extract, b2: 75% concentration of red onion extract, b3: 100% concentration of shallot extract. The results of research on bovine biourine influenced plant height, number of leaves at ages 7, 14, 21, 28.35 and 42 days after planting. The weight of the crop with the best results at the level of b2 (75% biourine cow) weighing 596.58 grams, onion extract affects plant height, number of leaves at 7, 14, 21, 28.35 and 42 hst, crop weight with the best results at the m2 level (75% red onion extract) with a weight of 567.14 grams and the interaction of the treatment of beef biourine and shallots affects plant height, number of leaves at the ages of 7, 14, 21, 28.35 and 42 days and days formed at the level b2m2 (75% beef biourine and 75 red onion extracts).

Drybalanops oblongifolia known as kayu kapur is a plant member of Dipterocarpaceae family. A neolignan, vladinol F, was successfully isolated from the dissolved fraction in acetone-ether of Dryobalanops oblongifolia stem bark. The chemical structure's determination was done based on an analysis of UV-Vis, NMR, and MS spectra and a comparison with the reference. In vitro test of this compound against Plasmodium falciparum 3D7 showed that IC50 value was 3.51 μg/mL.

Effect of Frequency and Application of Abamectin Insecticides on Population Abundance and Attack Level of Leafminer Liriomyza, spp. (Diptera: Agromysidae) is one of the main pests on the Lembah Palu red onion, where this pest is relatively new in the Palu Valley. The aim of the study was to examine the effect of frequency and application of insecticides on population abundance, and the attack rate of L. chinensis (Diptera: Agromyzidae) on the Lembah Palu red onion plant under applied and unapplied insecticide. The treatment of abamectin insecticide was applied once a week with a concentration of 1 ml of formulation per liter of water, beginning at the age of 2 MST and ending at 7 MST. The results showed that there were 2 species of Liriomyza, spp associated with Palu valley red onion i.e. Liriomyza chinensis and Liriomyza sativae. This research found that the application of Abamectin insecticides significantly affected population development and the percentage of Liriomyza, spp. on the lembah Palu red onion.