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Background: We hypothesized that a diet with old man saltbush hay used as an alternative source of nutrients could partially replace the concentrate in the feeding of feedlot lambs. Objective: This study evaluated the behavior and performance of lambs fed diets containing increasing levels of old man saltbush hay plus concentrate. Methods: Twenty-four castrated Santa Inês lambs at approximately eight months of age (22 ± 1.97 kg) were confined in a randomized complete design and fed diets containing 30, 40, 50, and 60% (dry matter: DM) of old man saltbush hay. Results: The intake of DM and neutral detergent fiber was not affected (p>0.05) by the level of old man saltbush hay. Intake of mineral salt decreased (p0,05) por los diferentes niveles de inclusión de hierba-sal. La ingestión de sal mineral se redujo (p0,05) pelos diferentes níveis de erva-sal. A ingestão de sal mineral reduziu (p

Background Evidence indicates a strong link between Zika virus (ZikV) and neurological complications. Acute myelitis, optic neuritis, polyneuropathy, and encephalomyelitis that mimic inflammatory idiopathic demyelination disorders (HDD) after ZikV infection have been reported in Brazil. Objective The present study aims to investigate the possible occurrence of molecular mimicry between ZikV antigens and Multiple Sclerosis (MS) autoantigens, the most frequent HDD of the central nervous system (CNS). Methods A retrospective cohort study with 305 patients admitted due to suspected arbovirus infection in Rio de Janeiro was performed, all subjects were submitted to neurological examination, and a biological sample was collected for serologic and molecular diagnostic. Bioinformatics tools were used to analyze the peptides shared between ZikV antigens and MS autoantigens. Results Of 305 patients, twenty-six were positive for ZikV and 4 presented IDD patterns found in MS cases. Sequence homology comparisons by bioinformatics approach between NS5 ZikV and PLP MS protein revealed a homology of 5/6 consecutive amino acids (CSSVPV/CSAVPV) with 83% identity, deducing a molecular mimicry. Analysis of the 3D structures revealed a similar conformation with alpha helix presentation. Conclusions Molecular mimicry between NS5 Zika virus antigen and PLP MS autoantigens emerge as a possible mechanism for IDD spectrum in genetically susceptible individuals. Resumo Antecedentes Evidências indicam uma forte ligação entre o vírus Zika (ZikV) e complicações neurológicas. Mielite aguda, neurite óptica, polineuropatia e encefalomielite que mimetizam distúrbios inflamatórios de desmielinização idiopáticos (DDII) após infecção por ZikV têm sido relatadas no Brasil. Obejtivo O presente estudo tem como objetivo investigar a possível ocorrência de mimetismo molecular entre antígenos do ZikV e autoantígenos da Esclerose Múltipla (EM), a DDII mais frequente do sistema nervoso central (SNC). Métodos Foi realizado um estudo de coorte retrospectivo com 305 pacientes internados por suspeita de infecção por arbovirus no Rio de Janeiro, todos os indivíduos foram submetidos a exame neurológico e coleta de amostra biológica para diagnóstico sorológico e molecular. Ferramentas de bioinformática foram usadas para analisar os peptídeos compartilhados entre antígenos do ZikV e autoantígenos da EM. Resultados Dos 305 pacientes, vinte e seis foram positivos para ZikV e 4 apresentaram padrão IDD encontrado em casos de EM. As comparações de homologia de sequência por abordagem de bioinformática entre a proteína NS5 ZikV e PLP EM revelaram uma homologia de 5/6 aminoácidos consecutivos (CSSVPV/CSAVPV) com 83% de identidade, deduzindo um mimetismo molecular. A análise das estruturas 3D revelou uma conformação semelhante com apresentação em alfa-hélice. Conclusões O mimetismo molecular entre o antígeno NS5 do vírus Zika e o autoantígeno PLP da EM surge como um possível mecanismo para o espectro IDD em indivíduos geneticamente suscetíveis.

One of the primary constraints in upland rice cultivation is the disease blast (Magnaporthe oryzae), which can provide reduction up to 100% of the grain yield The use of silicon with benefi cial microorganisms (bioagents) can be an alternative for the control of this disease and to provide an increase in the productivity of the rice grain. The objective of this work was to study the eff ect of rates of silicon with bioagents in blast suppression and grain yield of upland rice. The methodology used was tests carried out in field conditions, in two different areas: Capivara and Palmital farms, during the growing season 2015/2016. The experimental design was in a split-plot scheme with four replications. In the main plots were the silicon fertilization rates (0, 2, 4 and 8 ton ha-1) and in the subplots was the bioagents (1-without bioagents, 2-Pseudomonas fluorescens, 3-Burkholderia pyrrocinia, 4-Trichoderma asperellum, 5-a mixture of the three bioagents). The results showed that the use of 2 ton ha-1 of silicon with a mixture of bioagents was the best treatment to control leaf blast. Besides, from rates, 2 to 6 ton ha-1 of silicon in Capivara Farm and up to 8 ton ha-1 of silicon in Palmital Farm provided the highest grain yield. A mixture of bioagents provided the highest grain yield. In this sense, it was concluded that the best recommendation to connect blast control, grain yield and reduced amount of silicon was the use of 2 ton ha-1 of silicon with the mixture of bioagents. Made available in DSpace on 2021-12-02T14:00:55Z (GMT). No. of bitstreams: 1 jpsp-2021.pdf: 463307 bytes, checksum: a70c96be86c141bc7648f0b3558d6b5d (MD5) Previous issue date: 2021

This study evaluated the effects of different pre-slaughter road transportation times on sensory evaluation and instrumental measurements of Nellore cattle meat quality. About 15 farms were classified according to transportation times (five farms per transportation time group) as follows: 1 — less than 2 h (2 and ≤4), and 3 — between 4 and 6 h (>4 and ≤6). The experiment was a completely randomized design with 15 replications and three treatments. Meat analyses were performed on steaks taken from the longissimus dorsi muscle from the 10th rib to the third lumbar vertebra. Road transportation times had no significant effects on pH and mean shear force values. Sarcomere length, myofibrillar fragmentation index, and creatine kinase levels were also not affected by transportation time. Furthermore, road transportation times had no effect on meat tenderness, flavour, odour, and overall acceptability when evaluated by consumers. Thus, a pre-slaughter road transportation time up to 6 h does not affect the sensory evaluation and instrumental measurements of Nellore cattle meat quality when raised using a grazing system.

ATP-binding cassette (ABC) transporters perform multiple functions in reproductive tissues. During ovarian tissue vitrification, the plasma membrane has important functions in the influx or efflux of water, and substances such as cryoprotectants and channel proteins that are required in this process. Thus, the present study aimed to verify the relative abundance of mRNA transcript of ABC transporters ABCB1, ABCG2, and MRP2 after vitrification and in vitro culture (IVC) of ovine ovarian tissue. For this study, the ovarian cortex fragments were proportioned into four groups as fresh control, vitrified control, fresh culture, and vitrified culture groups. After vitrification and in vitro culture, the ovarian tissue was evaluated using morphological procedures. Further, relative abundance of ABCB1, ABCG2, and MRP2 transporter mRNA transcripts in the ovarian cortex subjected to aforementioned treatment conditions were evaluated using qPCR. Our results showed a negative association between degenerated follicles and mRNA transcript abundances of ABCB1 and ABCG2. In addition, the percentage of growing follicles in the ovine ovarian cortex after vitrification was similar to that of the fresh control tissue without in vitro culture. The in vitro culture of fresh and vitrified tissue however, showed a significant decrease in the percentage of growing follicles. To the best of our knowledge, we believe that our data for the first time has studied the relative abundances of ABCB1 and ABCG2 mRNA transcripts in the ovine ovarian cortex. In addition, alterations of these protein channels may be indicative of a deleterious effect of osmotic stress on follicular survival during vitrification. Furthermore, these effects were detectable only after the IVC of the ovarian tissues. Nonetheless, further studies are required to investigate the functions of ABC transporters in ovine folliculogenesis, especially after in vitro culture of ovarian tissue.

The availability of public genomic resources can greatly assist biodiversity assessment, conservation, and restoration efforts by providing evidence for scientifically informed management decisions. Here we survey the main approaches and applications in biodiversity and conservation genomics, considering practical factors, such as cost, time, prerequisite skills, and current shortcomings of applications. Most approaches perform best in combination with reference genomes from the target species or closely related species. We review case studies to illustrate how reference genomes can facilitate biodiversity research and conservation across the tree of life. We conclude that the time is ripe to view reference genomes as fundamental resources and to integrate their use as a best practice in conservation genomics., Trends in Genetics, 39 (7), ISSN:0168-9525

This study aimed to determine the prevalence of ovine brucellosis in the states of Rio Grande do Norte, Paraíba, and Sergipe. A seroepidemiological survey was carried out in 121 properties under 23 municipalities between 2011 and 2012. The 1,034 serological samples collected from the sheep were subjected to the agar gel immunodiffusion (AGID) test using kits produced by the Paraná Institute of Technology (TECPAR). Anti-Brucella ovis antibodies were observed in 5.37% (70/1,304) of the animals evaluated, and the difference in the incidence of brucellosis between the adults and young animals of both sexes, and between the males and females was statistically significant (p < 0.05). The prevalence of brucellosis in Rio Grande do Norte, Paraíba, and was Sergipe 7.66% (36/470), 5.40% (13/241) and 3.54% (21/593), respectively. Of the municipalities visited, 91.30% (21/23) harbored herds with positive serology, and 42.15% (51/121) of the properties had at least one seropositive animal. The lack of training among the workers (p ≤ 0.05) (odds ratio = 3.68; 95% CI = 1.08-12.60; p = 0.038) was identified as a risk factor. The results obtained in this study indicate that the sheep from Rio Grande do Norte, Paraíba, and Sergipe are infected by B. ovis, thus necessitating the adoption of proper sanitary measures for preventing the spread of the disease in the herds. [Caracterização epidemiológica e fatores de risco associados à infecção por Brucella ovis em ovinos dos estados do Rio Grande do Norte, Paraíba e Sergipe]. Resumo: O estudo teve como objetivo determinar a prevalência da Brucelose Ovina nos Estados do Rio Grande do Norte, Paraíba e Sergipe. Foi realizado o levantamento soroepidemiológico em 121 propriedades de 23 municípios durante os anos de 2011 e 2012. As 1.034 amostras sorológicas de ovinos foram submetidas à técnica de Imunodifusão em Gel de Ágar (IDGA), utilizando o kit produzido pelo Instituto de Tecnologia do Paraná (TECPAR). Anticorpos anti-Brucella ovis foram observados em 5,37% (70/1.304) dos animais testados, havendo diferença estatística significativa (p < 0,05) para adultos e jovens de ambos os sexos, e entre machos e fêmeas. A prevalência encontrada no Estado do Rio Grande do Norte, Paraíba e Sergipe foi de 7,66% (36/470), 5,40% (13/241) e 3,54% (21/593), respectivamente. Dos municípios visitados, 91,30% (21/23) eram compostos por rebanhos com sorologia positiva e 42,15% (51/121) das propriedades possuíam pelo menos um animal soropositivo. A falta de capacitação de trabalhadores (p ≤ 0,05) (odds ratio = 3,68; IC 95% = 1,08-12,60; p = 0, 038) foi identificada como fator de risco. Os resultados obtidos neste trabalho indicam que a infecção por B. ovis encontra-se presente nos ovinos dos estados do Rio Grande do Norte, Paraíba e Sergipe, sendo necessária a adoção de medidas sanitárias para evitar a propagação da doença nos rebanhos. Made available in DSpace on 2020-09-19T04:39:42Z (GMT). No. of bitstreams: 1 CNPC-2020-Art-12.pdf: 338704 bytes, checksum: 35ff880bb0934c5c66ed14faef33b4a0 (MD5) Previous issue date: 2020

Data availability: The datasets analyzed for the current study reflect collaborative efforts of two research consortia: ALLFTD and GENFI. Each consortium provides clinical data access based on established policies for data use: processes for request are available for review at allftd.org/data for ALLFTD data and by emailing genfi@ucl.ac.uk. Certain data elements from both consortia (for example raw MRI images) may be restricted due to the potential for identifiability in the context of the sensitive nature of the genetic data. The deidentified combined dataset will be available for request through the FTD Prevention Initiative in 2023 (https://www.thefpi.org/). Code availability: Custom R code is available at https://doi.org/10.5281/zenodo.6687486. Copyright © The Author(s). Unlike familial Alzheimer’s disease, we have been unable to accurately predict symptom onset in presymptomatic familial frontotemporal dementia (f-FTD) mutation carriers, which is a major hurdle to designing disease prevention trials. We developed multimodal models for f-FTD disease progression and estimated clinical trial sample sizes in C9orf72, GRN and MAPT mutation carriers. Models included longitudinal clinical and neuropsychological scores, regional brain volumes and plasma neurofilament light chain (NfL) in 796 carriers and 412 noncarrier controls. We found that the temporal ordering of clinical and biomarker progression differed by genotype. In prevention-trial simulations using model-based patient selection, atrophy and NfL were the best endpoints, whereas clinical measures were potential endpoints in early symptomatic trials. f-FTD prevention trials are feasible but will likely require global recruitment efforts. These disease progression models will facilitate the planning of f-FTD clinical trials, including the selection of optimal endpoints and enrollment criteria to maximize power to detect treatment effects. Data collection and dissemination of the data presented in this paper were supported by the ALLFTD Consortium (U19: AG063911, funded by the National Institute on Aging and the National Institute of Neurological Diseases and Stroke) and the former ARTFL and LEFFTDS Consortia (ARTFL: U54 NS092089, funded by the National Institute of Neurological Diseases and Stroke and National Center for Advancing Translational Sciences; LEFFTDS: U01 AG045390, funded by the National Institute on Aging and the National Institute of Neurological Diseases and Stroke). The manuscript was reviewed by the ALLFTD Executive Committee for scientific content. The authors acknowledge the invaluable contributions of the study participants and families as well as the assistance of the support staffs at each of the participating sites. This work is also supported by the Association for Frontotemporal Degeneration (including the FTD Biomarkers Initiative), the Bluefield Project to Cure FTD, Larry L. Hillblom Foundation (2018-A-025-FEL (A.M.S.)), the National Institutes of Health (AG038791 (A.L.B.), AG032306 (H.J.R.), AG016976 (W.K.), AG062677 (Ron C. Peterson), AG019724 (B.L.M.), AG058233 (Suzee E. Lee), AG072122 (Walter Kukull), P30 AG062422 (B.L.M.), K12 HD001459 (N.G.), K23AG061253 (A.M.S.), AG062422 (RCP), K24AG045333 (H.J.R.)) and the Rainwater Charitable Foundation. Samples from the National Centralized Repository for Alzheimer Disease and Related Dementias (NCRAD), which receives government support under a cooperative agreement grant (U24 AG021886 (T.F.)) awarded by the National Institute on Aging (NIA), were used in this study. This work was also supported by Medical Research Council UK GENFI grant MR/M023664/1 (J.D.R.), the Bluefield Project, the National Institute for Health Research including awards to Cambridge and UCL Biomedical Research Centres and a JPND GENFI-PROX grant (2019–02248). Several authors of this publication are members of the European Reference Network for Rare Neurologic Diseases, project 739510. J.D.R. and L.L.R. are also supported by the National Institute for Health and Care Research (NIHR) UCL/H Biomedical Research Centre, the Leonard Wolfson Experimental Neurology Centre Clinical Research Facility and the UK Dementia Research Institute, which receives its funding from UK DRI Ltd, funded by the UK Medical Research Council, Alzheimer’s Society and Alzheimer’s Research UK. J.D.R. is also supported by the Miriam Marks Brain Research UK Senior Fellowship and has received funding from an MRC Clinician Scientist Fellowship (MR/M008525/1) and the NIHR Rare Disease Translational Research Collaboration (BRC149/NS/MH). M.B. is supported by a Fellowship award from the Alzheimer’s Society, UK (AS-JF-19a-004-517). RC and C.G. are supported by a Frontotemporal Dementia Research Studentships in Memory of David Blechner funded through The National Brain Appeal (RCN 290173). J.B.R. is supported by NIHR Cambridge Biomedical Research Centre (BRC-1215-20014; the views expressed are those of the authors and not necessarily those of the NIHR or the Department of Health and Social Care), the Wellcome Trust (220258), the Cambridge Centre for Parkinson-plus and the Medical Research Council (SUAG/092 G116768); I.L.B. is supported by ANR-PRTS PREV-DemAls, PHRC PREDICT-PGRN, and several authors of this publication are members of the European Reference Network for Rare Neurological Diseases (project 739510). J.L. is funded by the Deutsche Forschungsgemeinschaft (German Research Foundation) under Germany’s Excellence Strategy within the framework of the Munich Cluster for Systems Neurology (EXC 2145 SyNergy – ID 390857198). R.S.-V. was funded at the Hospital Clinic de Barcelona by Instituto de Salud Carlos III, Spain (grant code PI20/00448 to RSV) and Fundació Marató TV3, Spain (grant code 20143810 to R.S.-V.). M.M. was, in part, funded by the UK Medical Research Council, the Italian Ministry of Health and the Canadian Institutes of Health Research as part of a Centres of Excellence in Neurodegeneration grant, by Canadian Institutes of Health Research operating grants (MOP- 371851 and PJT-175242) and by funding from the Weston Brain Institute. R.L. is supported by the Canadian Institutes of Health Research and the Chaire de Recherche sur les Aphasies Primaires Progressives Fondation Famille Lemaire. C.G. is supported by the Swedish Frontotemporal Dementia Initiative Schörling Foundation, Swedish Research Council, JPND Prefrontals, 2015–02926,2018–02754, Swedish Alzheimer Foundation, Swedish Brain Foundation, Karolinska Institutet Doctoral Funding, KI Strat-Neuro, Swedish Dementia Foundation, and Stockholm County Council ALF/Region Stockholm. J.L. is supported by Germany’s Excellence Strategy within the framework of the Munich Cluster for Systems Neurology (German Research Foundation, EXC 2145 Synergy 390857198). The Dementia Research Centre is supported by Alzheimer’s Research UK, Alzheimer’s Society, Brain Research UK, and The Wolfson Foundation. This work was supported by the National Institute for Health Research UCL/H Biomedical Research Centre, the Leonard Wolfson Experimental Neurology Centre Clinical Research Facility and the UK Dementia Research Institute, which receives its funding from UK DRI Ltd, funded by the UK Medical Research Council, Alzheimer’s Society, and Alzheimer’s Research UK.

Introduction: Vitiligo is a dermatological disease that affects about 0.38% to 2.9% of the world population. Currently, the main treatments used for vitiligo involve the use of topical drugs such as corticosteroids and calcineurin inhibitors, phototherapy, systemic treatment with steroids and even surgical grafts and, in acute cases, depigmenting treatments. Natural products are an alternative for the treatment of vitiligo: mamacadela (Brosimum gaudichaudii), a plant rich in furanocoumarins, and sowthistle (Sonchus oleraceus), rich in phenolic substances, are already used to treat vitiligo. There are also popular reports of the use of a preparation containing coffee (Coffea sp) and sunflower seed (Helianthus annuus) to treat vitiligo. Case report: A female patient, 28 years old, diagnosed with vitiligo, reported having obtained a positive result in the repigmentation of the pale white patches after the daily use of a preparation containing coffee and sunflower seed for about one year. Discussion: Data from the scientific literature demonstrated that chemical constituents of these plants, such as chlorogenic acid and its isomers, which have antioxidant and anti-inflammatory action, and substances such as linoleic acid and vitamins E and B, which help in the process of melanin formation on the skin, may be responsible for the observed repigmentation of the patches. Further research on this case report is important for scientific validation and the development of new therapeutic options, especially with less adverse effects, in the treatment of vitiligo.

In this work, we investigated in vitro the antioxidant, cytotoxic and anti-leishmanial activities of a lignin extracted from the leaves of Morinda citrifolia. Initially, an analysis of the composition of the sheets was performed, then the lignin was obtained by alkaline delignification and characterized by different techniques: elemental analysis, FT-R, UV–vis, HSQC-NMR, thermal analysis, Py-GC/MS and by GPC. The results showed that the leaves had in their composition cellulose (31.29%), hemicellulose (25.01%), lignin (18.34%), extractives (14.39%) and ash (10.03%). The lignin extraction yield was 89.8%. The lignin obtained is of the GSH type with the following contents 79.39%, 13.58% and 7.03% respectively. Furthermore, it is low molecular weight and thermally stable. It had a phenolic content of 93.3 mg GAE/g and low antioxidant activity. In macrophage cytotoxicity assays, it presented a CC50 of 31.0 μg/mL, showing less toxicity than amphotericin B. In assays against the promastigote forms of Leishmania amazonensis, lignin presented an IC50 of 29.56 μg/mL, a less effective concentration than amphotericin B (IC50 = 0.14 μg/mL). However, it was able to promote inhibition of the parasites, a fact confirmed by structural changes. These findings reinforce that M. citrifolia lignin is a promising macromolecule for use as an antiparasitic and antioxidant agent.

In September 2020, we tested 13,398 persons in Portugal for antibodies against severe acute respiratory syndrome coronavirus 2 by using a quota sample stratified by age and population density. We found a seroprevalence of 2.2%, 3–4 times larger than the official number of cases at the end of the first wave of the pandemic.

Although Echinostoma robustum (currently a synonym of E. miyagawai) was reported in the Americas based on molecular data, morphological support on adult parasites is still required. Herein, a new species of Echinostoma is described based on worms found in a chicken from Brazil. Molecular phylogenetic analyses based on 28S (1063 bp), ITS (947 bp) and Nad-1 (442 bp) datasets reveal the inclusion of the new species within Echinostoma ‘revolutum’ species complex. Moreover, it was verified the conspecificity between cercariae previously identified as E. robustum in Brazil [identical ITS and only 0.3% of divergence (1 nucleotide) in Nad-1]. Species discovery analyses show that these two isolates form an independent lineage (species) among Echinostoma spp. Compared to E. miyagawai, the new species presents relatively high divergence in Nad-1 (7.88–9.09%). Morphologically, the specimens are distinguished from all nominal species from the ‘revolutum’ species complex by the more posterior position of the testes (length of post-testicular field as a proportion of body length about 20%). They further differ from E. miyagawai and South American Echinostoma spp. by the higher proportion of forebody to the body length. Therefore, combined molecular and morphological evidence supports the proposal of the species named here as Echinostoma pseudorobustum sp. nov.

Ovarian tissue transplantation methods using cooled and cryopreserved samples have been attractive options for fertility preservation in animal models and humans. The aim of this study was to evaluate the impact of previous exposure to cooling, cryopreservation, and VEGF on the overall efficiency of equine ovarian tissue after heterotopic xenotransplantation in mice. The end points evaluated were follicular morphology and development, follicular and stromal cell densities, angiogenesis (i.e. the density of new and mature blood vessels), collagen types I and III fiber densities, and total fibrosis. Ovaries of adult mares were harvested after ovariectomy, and ovarian fragments were xenografted in the i.p. wall of BALB nude mice. Ten types of treatments involving different combinations of cooling, cryopreservation, xenografting procedures, and VEGF exposure were compared. The novel aspect of this study was the use of equine ovarian tissue xenotransplantation in mice, challenging the fragments with different combinations of treatments. The main findings were (i) cooling but not cryopreservation was effective in preserving the follicular morphology, (ii) a greater percentage of developing follicles but lower follicular and stromal cell densities were observed after ovarian tissue engraftment, (iii) exposure to VEGF increased new and mature vessels in cryopreserved-transplanted tissue, and (iv) an appropriate balance in the collagen types I and III fiber ratio in cooling-transplanted tissue was observed after exposure to VEGF. This study contributes to advancing knowledge in the preservation of ovarian tissue after cooling-cryopreservation and transplantation aiming to be applied to genetically superior/valuable horses, livestock, endangered animals, and, possibly, humans. Lay summary Due to ethical limitations involving humans, the female horse (mare) has recently emerged as an alternative model for reproductive comparisons with women to optimize fertility restoration using ovarian tissue transplantation techniques. This study determined if ovarian tissue from donor mares (n = 3), exposed or not to vascular endothelial growth factor (VEGF) before transplantation, better survives for 7 days after transplantation into mouse hosts (n = 12). Tissues submitted to different combinations of cooling, freezing, and transplanting treatments, along with control groups, were evaluated using the parameters morphology, development, the density of immature eggs (follicles), the density of supportive (stromal) cells, collagen protein proportions, and density of blood vessels. Frozen-thawed treatments had lower percentages of normal follicles. Exposure to VEGF increased blood vessel densities in frozen tissue and favored adequate collagen levels in cooled-transplanted treatments. In conclusion, VEGF exposure seems to be beneficial for mare ovarian tissue transplantation and warrants further investigation.

Cereal crops strongly contribute with the world economy and because of this the reduction of their productivity may lead to irreparable economic consequences worldwide. The maize weevil Sitophilus zeamais is the main pest of maize in the field or during processing, storage and commercialization of grains, also attacking processed food. The population control of S. zeamais uses a restrict panel of synthetic insecticides which are associated with environmental contamination, selection of resistant individuals and toxicity to non-target organisms, including humans and other animals. This scenario has stimulated the search for new insecticides and plant metabolites stand out because of their high degree of biodegradability and more selective toxicity. This work provides a review of the effect of plant compounds on S. zeamais to encourage the use of these ingredients in more ecofriendly strategies for pest control. Plant insecticides can exert their toxicity by ingestion, contact and/or fumigation resulting in damage to the development, survival and reproductive potential of S. zeamais, or may present food deterrent activity, which protects the substrates from the attack by the insects. The data reviewed contribute to consolidate the use of natural products in control of S. zeamais.

Developing salt-tolerant plants has emerged as a highly efficient approach to cope with salinity damage on crop growth and productivity. This study aimed to elucidate the mechanisms of salt acclimation in cowpea plants [Vigna unguiculata (L.) Walp] and screen salt-tolerant potential genotypes during the early vegetative stage. Seven cowpea genotypes (Epace, Jurua, Marataua, Milagroso, Pitiuba, Sempre verde, and TVU) were irrigated for 24 days with saline solutions of electrical conductivity 0.8 (control), 4.0 (moderate stress), and 8.0 dS m−1 (severe stress). Growth, water status, membrane damage, and variables related to photosynthetic machinery efficiency were evaluated. Biomass accumulation dramatically decreased with salinity, and the reductions were intensified by increasing the salt level. Nevertheless, under moderate salinity, Pitiuba plants showed less reductions in growth than other genotypes. Under moderate stress, Pitiuba plants exhibited maintenance of osmotic potential and photosynthetic pigments, which was consistent with unaltered membrane and elevated leaf succulence, resulting in improved photochemical performance. Conversely, although TVU, Jurua, and Milagroso plants had activated responses against moderate salinity, including reduced leaf osmotic potential and improved stomatal conductance, these responses were not sufficient to mitigate salt injury. The findings clearly show that the Pitiuba genotype activates coordinated responses to mitigate moderate salt damage, constituting an alternative for cultivating cowpea plants in saline environments.

Rhipsalis baccifera (Sol.) Stearn is a typical cactus from tropical regions with wide geographic distribution, and its therapeutic potential is not yet fully understood, such as antitumoral property. Thus, this study evaluated the cytotoxic ethanolic extract of R. baccifera (EERB) and its antitumor activity against Erlich's tumor in mice. The EERB was obtained, and its phytochemical profile was filed by thin-layer chromatography. The toxicity was evaluated in vitro and in vivo using the microcrustacean Artemia salina Leach and mice. The lethal dose was determined after implantation of a tumor cell suspension, with subsequent treatment with EERB (200 mg/kg and 100 mg/kg) 48h after implantation. These values represent the tenth part of the DL50 and CL50, respectively. The presence of phenols, tannins and triterpenes were demonstrated in the phytochemical results. Toxicity was dose-dependent, and the tumor inhibition was 84.1% and 75.8% at doses of 200 mg/kg and 100 mg/kg, respectively. We can highlight that the growth of Erlich's carcinoma suffered inhibitory effects against the EERB. EERB was found to have low acute toxicity and a high potential for use in antitumor therapy. Thus, new studies involving pre-clinical and clinical analyses of the extract are essential to determine the safe dose.

Chiroptera stands out for the richness of species and a variety of unique characteristics. However, the morphological similarities in some taxa of this group can result in neglected diversity, as pointed out in several molecular studies. In this sense, the DNA barcode has been useful in the identification and delimitation of species. In the state of Parana, in the southern region of Brazil, molecular studies in this group are rare and many species are not registered in the public genetic database. Thus, we identified at the molecular level Chiroptera species that occur in the northwestern region of Parana and compared the data obtained with species deposited in the Barcode of Life Database (BOLD), in addition to carrying out OTUs delimitation tests. We conclude that Chiroptera has an underestimated diversity in Parana, with some species awaiting description. Our results suggest the existence of poorly resolved taxa, and in general the group needs new studies with different approaches to solve its taxonomy.

Introduction Cocaine use disorder is a significant public health issue without a current specific approved treatment. Among different approaches to this disorder, it is possible to highlight a promising immunologic strategy in which an immunogenic agent may reduce the reinforcing effects of the drug if they are able to yield sufficient specific antibodies capable to bind cocaine and/or its psychoactive metabolites before entering into the brain. Several carriers have been investigated in the anti-cocaine vaccine development; however, they generally present a very complex chemical structure, which potentially hampers the proper assessment of the coupling efficiency between the hapten units and the protein structure. Objectives The present study reports the design, synthesis and preclinical evaluation of two novel calix[n]arene-based anti-cocaine immunogens (herein named as V4N2 and V8N2) by the tethering of the hydrolysis-tolerant hapten GNE (15) on calix[4]arene and calix[8]arene moieties. Methods The preclinical assessment corresponded to the immunogenicity and dose–response evaluation of V4N2 and V8N2. The potential of the produced antibodies to reduce the passage of cocaine analogue through the blood–brain-barrier (BBB), modifying its biodistribution was also investigated. Results Both calix[n]arene-based immunogens elicited high titers of cocaine antibodies that modified the biodistribution of a cocaine radiolabeled analogue (99mTc-TRODAT-1) and decreased cocaine-induced behavior, according to an animal model. Conclusion The present results demonstrate the potential of V4N2 and V8N2 as immunogens for the treatment of cocaine use disorder.

Restaurantes self-service são muito procurados pelos consumidores que costumam se alimentar fora do lar. A alface é uma das hortaliças mais consumidas no Brasil, principalmente crua, sendo importante sua higienização, para evitar doenças alimentares. Este estudo objetivou avaliar a qualidade microbiológica e parasitológica de alfaces (Lactuca sativa) prontas para consumo, e a qualidade físico-química e microbiológica da água de consumo de restaurantes tipo self-service no município de Patos, Paraíba. Foram coletadas amostras de alface e água de 25 restaurantes. Nas amostras de alface foram realizadas a contagem de coliformes totais e termotolerantes e Staphylococcus coagulase positiva, e a pesquisa de Salmonella, Escherichia coli e parasitos. Nas amostras de água foram feitas avaliações de coliformes totais e Escherichia coli e análises físico-químicas de cor, turbidez e pH. Foi constatado que 100% das amostras analisadas apresentaram coliformes totais, e 60% apresentaram coliformes termotolerantes acima do tolerado pela legislação brasileira. Foi detectada a presença de Salmonella spp. em 12% das amostras de alface e oocistos de protozoários em 4% e 56% das amostras de água estavam impróprias para consumo. Os estabelecimentos estudados apresentaram condições higiênico-sanitárias insatisfatórias. Palavras-chave: Verduras. Risco Sanitário. Coliformes. Parasitos. Qualidade da Água. Abstract Self-service restaurants are much sought after by consumers who tend to eat out. Lettuce is one of the most consumed vegetables in Brazil, mainly raw, and its sanitization is important, in order to avoid foodborne diseases. This study aimed to evaluate the microbiological and parasitological quality of lettuces (Lactuca sativa) ready for consumption and the physical-chemical and microbiological quality of the drinking water of the self-service restaurants in the municipality of Patos, Paraíba. Samples of lettuce and water were collected from 25 restaurants. In the samples of lettuce were carried out the count of total and thermotolerant coliforms and coagulase positive Staphylococcus, and the also the search for Salmonella, Escherichia coli and parasites. In the samples of water was made the evaluations of total coliforms and Escherichia coli and physical and chemical analysis of color, turbidity and pH. It was found that 100% of the samples of lettuce presented total coliforms, and 60% presented thermotolerant coliforms above the tolerated by the Brazilian legislation. The presence of Salmonella spp. was detected in 12% of the samples of lettuce and protozoan oocysts in 4%. In addition, 56% of the samples of water were unfit for consumption. The establishments studied presented unsatisfactory hygienic and sanitary conditions. Keywords: Vegetables. Health Risk. Coliforms. Parasites. Water Quality.

Seed processing and dormancy break treatments are recommended for rendering seeds restoration-ready. Conversely, fruit structures and seed coats may protect seeds from environmental harm in the field. We evaluated the effects of seed processing (by either keeping fruit structures or extracting seeds) and/or scarification (of physically dormant seeds) on the seedling emergence and establishment of 10 legume tree species from tropical forests and savannas of Central Brazil. We sowed seeds in a greenhouse for reference and in a field experiment conducted in tilled ready-to-seed sites. We monitored seedling emergence and survival for a year. We calculated the costs of harvesting, processing, and pretreating seeds, and considered the final cost of a 1-year-old seedling. Seed extraction resulted in lower emergence for most species in the greenhouse and in the field. It also accelerated emergence of three and four species in the greenhouse and the field, respectively. Scarification resulted in lower seedling emergence in the field for half of the species, while it increased emergence of three species in the greenhouse. Most species presented accelerated emergence both in the greenhouse and the field. The seedling cost was 1.6 to 74.6 times higher when seeds were processed, and 1.3 to 6.0 times when seeds were scarified, except for one species. Keeping fruit structures and seed coats reduced the costs of seeds and increased the success of direct seeding.

Introduction: Inflammatory Bowel Disease (IBD), periodontitis and Systemic Lupus Erythematous (SLE) are multifactorial diseases, one of the factors in the course of these diseases is the rs333 polymorphism in the CC chemokine receptor type five (CCR5) gene. However, the results remain contradictory. Therefore, we aimed to perform a meta-analysis evaluating the relation between this polymorphism and the aforementioned conditions. Material and Methods: A search in the literature was performed in diverse scientific and medical databases for studies published before June 22, 2020. The data were extracted from the studies and the statistical evaluation was performed by the calculations of statistical heterogeneity (I²), Odds Ratio (OR) with 95% of Confidence Intervals (CI) and publication bias. The values of P

The present study evaluated the general welfare state of two strains of transgenic goats bred in a region with a hot and humid tropical climate. Nine females were used, being three transgenic for human lysozyme (hLZ group), three transgenic for human glucocerebrosidase (hGCase group), and three non-transgenic (control group). The temperature and humidity index (THI) were recorded during the morning, afternoon, and evening. The physiological parameters measured were respiratory rate, heart rate, and rectal and vaginal temperatures. Venous blood samples were collected using Vacutainer® tubes containing 10% ethylenediaminetetraacetic acid (EDTA). Also, analysis of erythrogram, leukogram, and some biochemical parameters of serum was performed. It was observed that the afternoon shift presented the largest THI, being potentially more impactful on the physiology of animals. In general, respiratory and heart rates were higher in transgenic animals, especially in the hLZ group compared to the control group (P

The diversity of endotherms and ectotherms may be differently affected by ambient temperature and net primary productivity (NPP). Additionally, little is known about how these drivers affect the diversity of guilds of different trophic levels. We assessed the relative role of temperature and NPP in multitrophic guilds of ectothermic (arthropods: ants, ground beetles, spiders, and harvestmen) and endothermic (large mammals) animals along a tropical elevational gradient. We sampled arthropods at eight elevation belts and large mammals at 14 elevation belts in Atlantic rainforest (ranging from 600 to 2450 m.a.s.l.) of Itatiaia National Park, Southeast Brazil. Overall arthropod species richness was more associated with temperature than overall large-mammal species richness, while the latter was more associated with NPP. When separated into trophic guilds, we found that the species richness associated with NPP increased across arthropod trophic levels from herbivores to predators. Conversely, although NPP influenced large-mammal herbivore species richness, its effects did not seem to accumulate across large-mammal trophic levels since the species richness of large-mammal omnivores was more associated with temperature and none of the variables we studied influenced large-mammal predators. We suggest that thermal physiological differences between ectotherms and endotherms are responsible for the way in which arthropods and large mammals interact with or are constrained by the environment. Furthermore, the inconsistency regarding the role of temperature and NPP on species richness across multitrophic guilds of ectotherms and endotherms could indicate that thermal physiological differences might also interfere with energy use and flux in the food web.

Introduction Exercise is a key factor in immunity which may be affected by a different diet intake. Thus, we aimed to analyze the effects of diet intake and resistance training on spleen structure of ovariectomized female animals. Materials and Methods Female Wistar rats were divided into eight experimental groups: sedentary and nonovariectomized animals plus vegetable protein diet (CVS) or animal protein diet (CAS); trained and nonovariectomized rats plus vegetable protein diet (CVT) or animal protein diet (CT); sedentary and ovariectomized groups plus vegetable protein diet (VOS) or animal protein diet (AOS); and trained and ovariectomized animals plus vegetable protein diet (VOT) or animal protein diet (AOT). Results Groups submitted to both animal protein diet and resistance training, mainly ovariectomized groups, presented a great variability of collagen fibers type III, white pulp, and follicle structure in relation to the other spleen constituents. Conclusion Resistance training with a vegetable protein diet may play a key factor to maintain spleen’s immune responses across age. However, animal protein diet is suggested to decrease spleen’s immune activity.

The objective of this study was to evaluate the effect of exposure to different colors of light during egg incubation on the reproductive parameters of male and female Japanese quails. A total of 1776 eggs were incubated under four lighting conditions for 24 h a day during the entire incubation period: white LEDs, green LEDs, red LEDs and darkness (control). The experimental design was a randomized block (incubation time) with four treatments of six replicates of two cages each. After hatching, the birds were housed in brood cages with 18 birds each to 35 days of age, when they were sexed and transferred to production cages for evaluation of reproductive parameters. After the onset of laying, the number of eggs in each cage was recorded daily, and the values were used to obtain the age of the females at first egg and at 80% laying. At 35 and 60 days of age, several birds from each cage were euthanized for anatomical and histological evaluation of the gonads. Two females from each cage were weighed every three days until 60 days of age to determine the growth curve. After 60 days, eggs from each cage were collected and assessed for external and internal quality. At 70, 74 and 78 days of age, semen collection was performed and seminal quality was evaluated. Then, the males were transferred to cages containing 9 females for the fertility test. Hatchability was higher (P 0.05) in eggs incubated in the dark and under the red LED. The age of maximum growth was higher (P 0.05) in birds from eggs incubated in the dark and under the white LED. There was no difference (P 0.05) in the anatomical and histological characteristics of the testicles between the groups incubated under different light colors, except for the diameter of the seminiferous tubules, which was greater (P = 0.05) in the dark and in the white LED groups. There was no effect (P 0.05) of light color during incubation on the productive index or egg quality of adult birds. There was also no effect (P 0.05) on sperm quality, except for sperm motility, the values of which were higher (P 0.05) in birds from eggs incubated in different colors of light. However, this difference was not sufficient to significantly (P 0.05) influence bird fertility. It is concluded that under the studied conditions, the incubation of quail eggs under white, red, and green LED lamps does not influence the reproductive characteristics of the quails.

Leaf area measurements are used in agronomic studies to evaluate plant growth, light interception, photosynthetic rates, and plant transpiration. It constitute an important indicator of crop productivity, for which the evaluation method must be fast, accurate, and of low cost. The objective of this study was to compare different indirect methods to estimate leaf area in pornunça (Manihot sp.). The research was carried out under field conditions from August 2017 to January 2019 in the semiarid region of Pernambuco State, Northeastern Brazil. Three methods were tested: linear dimensions of leaf (length, width, and the number of lobes), digital image, and leaf scanned image, analyzing 150 healthy leaves from 120 plants of pornunça at different growth stages. The criteria used to determine the best model(s) were a high coefficient of determination, low Akaike information criterion, low sum of squares of residuals, and high Willmott index. Independent of the method of determination, the power models showed the best criteria of adequacy for estimating the leaf area of the pornunça. The digital image, using the power model (Y=LW0.77NL0.49, where L and W are the leaf length and width, and NL is the number of lobes in the leaf) was the best non-destructive method for estimating the leaf area in pornunça plants.

Clostridium septicum (CS) is a pathogen that can cause the death of animals in livestock worldwide through its main virulence factor, alpha-toxin (ATX). The aspects involved in diseases caused by ATX, such as economic impact, prevalence, and rapid clinical course, require that animals should be systematically immunized. This review provides an overview of CS in livestock farming and discusses current immunization methods. Currently, commercial vaccines available against CS involve the cultivation and inactivation of microorganisms and toxins using a time-consuming, expensive, and high biological risk-carrying production platform, and some have been reported to be ineffective. An alternative to this process is the recombinant DNA technology, although recombinant ATX obtained thus far is no longer efficient in stimulating protective antibody titers despite improvements in the production methods. On the other hand, immunized animals have highly favorable levels of survival when subjected to challenge tests, suggesting that high titers of circulating serum antibodies may not be representative of protection after immunization and that the non-immune cellular defenses associated with the particularities of the mechanism of action of ATX may be involved in the immune response of the host. To contribute to the future of global livestock farming through the development of more efficient recombinant vaccines, we suggest novel perspectives and strategies, such as the location of immunodominant epitopes, expression of relevant functional domains, and construction of chimeras, in the rational design of recombinant ATX.

Background Cystic echinococcosis (CE) is a worldwide parasitic zoonosis caused by the larval stage of Echinococcus granulosus sensu lato affecting livestock, particularly sheep and goats. However, often this parasitosis is underestimated. For this reason, this study aimed to evaluate the epidemiological features and spatial distribution of CE in sheep and goats slaughtered in a hyperendemic Mediterranean area. Methods A survey was conducted in the Basilicata region (southern Italy) from 2014 to 2019. A total of 1454 animals (1265 sheep and 189 goats) from 824 farms were examined for hydatid cyst detection by visual inspection, palpation and incision of target organs. All the CE cysts were counted and classified into five morphostructural types (unilocular, multiseptate, calcified, caseous and hyperlaminated). Molecular analysis was performed on 353 cysts. For spatial analysis, a kriging interpolation method was used to create risk maps, while clustering was assessed by Moran’s I test. Results CE prevalence of 72.2% (595/824) and 58.4% (849/1454) was observed at the farm and animal levels, respectively, with higher values in sheep (62.9%) than goats (28.0%). The liver and lungs were the most frequently infected organs in both sheep and goats. Most of recovered cysts were of the calcified and multiseptate morphotypes. All the isolates were identified as E. granulosus sensu stricto (genotypes G1–G3). Spatial distribution showed a moderate clustering of positive animals. Conclusion The findings of this study can be used to better understand the eco-epidemiology of echinococcosis and to improve CE surveillance and prevention programs in regions highly endemic for CE. Graphical abstract

RESUMO No Nordeste brasileiro, cuja vegetação predominante é Caatinga, muitas plantas nativas ou exóticas são potencialmente ricas em propriedades curativas, porém pouco exploradas ainda pela ciência. O trabalho foi realizado durante agosto de 2011 a junho de 2012, onde foram coletadas amostras bacterianas dos tetos dos animais através de swabs estéreis. Isolados e devidamente identificados, os microrganismos foram sujeitos a antibiogramas com os meios alternativos, controles positivos e negativo através da técnica de difusão em Ágar de poço. O objetivo do trabalho foi avaliar a ação dos extratos de Favela (Cnidoscolus phyllacanthus), Catingueira (Caesalpinia pyramidalis) e Nim (Azadiracta indica) sobre bactérias isoladas de tetos de cabras no assentamento de Cordão de Sombra no Município de Mossoró-RN. ABSTRACT In Northeast Brazil, whose predominant vegetation is Caatinga, many native or exotic plants are potentially rich in healing properties, but little explored yet by science. The work was conducted during August 2011 to June 2012, where bacterial samples were collected from the teats of animals through sterile swabs. Once isolated and properly identified, the microorganisms were subjected to antibiograms with alternative means, positive and negative controls through the well agar diffusion technique. The objective of the work was to evaluate the action of extracts of Favela (Cnidoscolus phyllacanthus), Catingueira (Caesalpinia pyramidalis) and Neem (Azadiracta indica) on bacteria isolated from teats of goats in the settlement Cordão de Sombra in the Municipality of Mossoró-RN.

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This study describes the cellulases (CLS), hemicellulases (HCS), and reducing sugars (RS) production by Pleurotus ostreatus PLO6 growth under solid state fermentation (SSF) using stems and leaves mixture of Alstroemeria sp. (AW) as lignocellulosic (LC) substrate (also named biomass). SSF involved, at the same time, CLS and HCS biosynthesis, and its subsequent action over the substrate in order to obtain RS, which are considered as precursors of bioethanol and other bioproducts. The first stage of this research was performed to verify simultaneous obtaining of hemicellulolytic and cellulolytic enzymes, and RS by means of univariate experimentation (response variables: hemicellulolytic and cellulolytic enzyme activities and RS concentration, factor: time). In this preliminary study, P. ostreatus PLO6 was able to synthesize endoglucanases, exoglucanases, β-glucosidases, endoxylanases, β-xylosidases, and RS concurrently. Afterwards, temperature (T), substrate concentration (SC), and time (t) effects on simultaneous obtaining of hemicellulolytic and cellulolytic enzymes and RS were assessed. A statistical factorial design 23 was developed using the same response variables indicated at the preliminary phase. With a significance level of 0.05, temperature was the most significant effect on enzymatic activities (excepting β-xylosidases) and RS production. CLS activities and RS production were maximized at 24 °C, while HCS activities were better at 32 °C. The SC was statistically significant too, when it was maintained at 30%, the CLS (excepting endoglucanases) and HCS production was favorable. The RS highest production was of 44.8 mg·g−1 (respective to raw AW) and 124.3 mg·g−1 (respective to cellulose) at 24 °C, 20% of SC and 8 days of SSF.

We aimed to analyse the effects of Cryptostegia madagascariensis’ invasion on soil biota community composition from a tropical Fluvisol at the Brazilian Northeast. We collected soil organisms from two dissimilar environments (e.g., invaded and non-invaded) using Provid-type traps into transects. There was a positive correlation between the abundance of Araneae, Blattodea, Homoptera and, Pseudoscorpiones with the invaded environment. On the other hand, the frequency of occurrence of Hymenoptera was significantly higher in the invaded environment compared to the non-invaded one. The biological invasion process promoted by C. madagascariensis reduces soil biota diversity, and the frequency of occurrence of important functional groups, such as predators (e.g., Araneae, Dermaptera, and Scorpiones), and litter transformers (e.g., Coleoptera). This creates a negative plant-soil feedback that promote the functional redundancy process by soil organisms and the biological invasion process by the invader into the Caatinga ecoregion.

This study was to evaluate the biological activity of the extract of Botryosphaeria fabicerciana isolated from leaves of Morus nigra. The volatile compounds from the crude extract were analysed by GC-MS which demonstrate that mellein and β-orcinaldehyde were are the major compounds. The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract was observed against Gram-positive bacteria, with a MIC of 15.6 μg/mL towards B. cereus and MIC of 62.5 μg/mL towards S. aureus and B. subtilis. MBC values of 31.25 μg/mL, 62.5 μg/mL, and 250 μg/mL were observed towards B. cereus, B. subtilis, and S. aureus, respectively. The cytotoxicity analyses showed CC50 of 115 μg/mL. The crude extract showed antioxidant activity by the DPPH, ABTS, and FRAP assays. Therefore, the extract of the endophytic fungus presented biotechnological potential as an antibacterial and antioxidant agent.

In the present study, a range of serum biomarkers were quantified in suspected cases of adverse events following YF immunization (YEL-AEFI) to propose a reliable laboratorial algorithm to discriminate confirmed YEL-AEFI (“A1” class) from cases with other illnesses (“C” class). Our findings demonstrated that increased levels of CXCL8, CCL2, CXCL10, IL-1β, IL-6 and TNF-α were observed in YEL-AEFI (“A1” and “C” classes) as compared to primary vaccines without YEL-AEFI [PV(day 3–28)] and reference range (RR) controls. Notably, increased levels of CCL3, CCL4, CCL2, CCL5, IL-1β, IL-15, IL-1Ra and G-CSF were found in “A1” as compared to “C” class. Venn diagrams analysis allowed the pre-selection of biomarkers for further analysis of performance indices. Data demonstrated that CCL3, CCL5, IL-15 and IL-1Ra presented high global accuracy (AUC = 1.00) to discriminate “A1” from “C”. Decision tree was proposed with a reliable algorithm to discriminate YEL-AEFI cases according to cause-specific definitions with outstanding overall accuracy (91%). CCL3, CCL5, IL-15 and IL-1Ra appears as root attributes to identify “A1” followed by VEGF as branch nodes to discriminate Wild Type YFV infection (“C(WT-YFV)”) from cases with other illnesses (“C*”). Together, these results demonstrated the applicability of serum biomarker measurements as putative parameters towards the establishment of accurate laboratorial tools for complementary differential diagnosis of YEL-AEFI cases.

Beans are one of the main foods consumed in Brazil. It is considered an essential ingredient in the basic diet of Brazilians. As a management practice, aiming to supply N and increase crop productivity, producers have used the association of seed inoculation with nitrogen fertilization. Thus, the objective of this study is to evaluate the effects of split nitrogen fertilization and seed inoculation on common bean productivity. The experiment was carried out in completely randomized blocks and a 2x2x3 factorial design with four replications. The first factor was seed inoculation (presence or absence) with peat inoculant containing the strains SEMIA 4077 and SEMIA 4080 from R. tropici and the strain SEMIA 4088 from R. freirei. The second factor was the parceling of nitrogen cover fertilization (80+40 and 60+60 kg ha-1 of N at 20 and 40 days after emergence (DAE)). The third factor was time of assessment (50, 65 and 80 DAE). The inoculated bean showed a 5% higher leaf N content than non-inoculated ones. Transpiration and liquid photosynthesis rates were higher in inoculated plants and in those with 60+60 kg ha-1 of N. However, productivity was 13% higher in uninoculated beans regardless of nitrogen fertilization. Los frijoles son uno de los principales alimentos consumidos en Brasil. Se considera un ingrediente esencial en la dieta básica de los brasileños. Como práctica de manejo, con el objetivo de suministrar N y aumentar la productividad de los cultivos, los productores han utilizado la asociación de la inoculación de semillas con la fertilización con nitrógeno. Por lo tanto, el objetivo de este estudio es evaluar los efectos de la fertilización con nitrógeno dividido y la inoculación de semillas sobre la productividad del frijol común. El experimento se realizó en bloques completamente al azar y un diseño factorial 2x2x3 con cuatro repeticiones. El primer factor fue la inoculación de semillas (presencia o ausencia) con inoculante de turba que contenía las cepas SEMIA 4077 y SEMIA 4080 de R. tropici y la cepa SEMIA 4088 de R. freirei. El segundo factor fue la parcelación de la fertilización de la cubierta de nitrógeno (80 + 40 y 60 + 60 kg ha-1 de N a los 20 y 40 días después de la emergencia (DAE)). El tercer factor fue el tiempo de evaluación (50, 65 y 80 DAE). El frijol inoculado mostró un contenido de N en hojas 5% más alto que los no inoculados. Las tasas de transpiración y fotosíntesis líquida fueron mayores en plantas inoculadas y en aquellas con 60 + 60 kg ha-1 de N. Sin embargo, la productividad fue 13% mayor en frijoles sin inocular independientemente de la fertilización con nitrógeno. O feijão é um dos principais alimentos consumidos no Brasil. É considerado ingrediente essencial na dieta básica do brasileiro. Como prática de manejo, visando fornecer N e aumentar a produtividade das lavouras, os produtores têm utilizado a associação da inoculação de sementes à fertilização com nitrogênio. Assim, o objetivo deste trabalho é avaliar os efeitos da fertilização nitrogenada parcelada e da inoculação de sementes na produtividade do feijoeiro. O experimento foi conduzido em blocos ao acaso e em esquema fatorial 2x2x3 com quatro repetições. O primeiro fator foi a inoculação das sementes (presença ou ausência) com inoculante turfoso contendo as cepas SEMIA 4077 e SEMIA 4080 de R. tropici e a cepa SEMIA 4088 de R. freirei. O segundo fator foi o parcelamento da adubação com cobertura de nitrogênio (80 + 40 e 60 + 60 kg ha-1 de N aos 20 e 40 dias após a emergência (DAE)). O terceiro fator foi o tempo de avaliação (50, 65 e 80 DAE). O feijão inoculado apresentou teor de N foliar 5% maior do que o não inoculado. As taxas de transpiração e fotossíntese líquida foram maiores nas plantas inoculadas e naquelas com 60 + 60 kg ha-1 de N. No entanto, a produtividade foi 13% maior nos grãos não inoculados independentemente da fertilização com nitrogênio.