1. Use of Laccase as a novel, versatile reporter system in filamentous fungi
- Author
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Mander, Gerd J., Huaming Wang, Bodie, Elizabeth, Wagner, Jens, Vienken, Kay, Vinuesa, Claudia, Foster, Caroline, Leeder, Abigail C., Allen, Gethin, Hamill, Valerie, Janssen, Giselle G., Dunn-Coleman, Nigel, Karos, Marvin, Lemaire, Hans Georg, Subkowski, Thomas, Bollschweiler, Claus, Turner, Geoffrey, Nusslein, Bernhard, and Fischer, Reinhard
- Subjects
Aspergillus -- Research ,Fungi -- Research ,Bacterial genetics -- Research ,Biological sciences - Abstract
A report is presented on a novel application of laccases as a reported system in Aspergillus nidulans, Aspergillus niger and Trichoderma reesei. Monitoring of heterologously expressed laccase activity is done in colorometric enzyme assays and on agar plates with 2,2'-azino-di-(3-ethylbenzthiazolinsulfonate) (ABTS) as a substrate.
- Published
- 2006