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Use of Laccase as a novel, versatile reporter system in filamentous fungi

Authors :
Mander, Gerd J.
Huaming Wang
Bodie, Elizabeth
Wagner, Jens
Vienken, Kay
Vinuesa, Claudia
Foster, Caroline
Leeder, Abigail C.
Allen, Gethin
Hamill, Valerie
Janssen, Giselle G.
Dunn-Coleman, Nigel
Karos, Marvin
Lemaire, Hans Georg
Subkowski, Thomas
Bollschweiler, Claus
Turner, Geoffrey
Nusslein, Bernhard
Fischer, Reinhard
Source :
Applied and Environmental Microbiology. July, 2006, Vol. 72 Issue 7, 5020-5026
Publication Year :
2006

Abstract

A report is presented on a novel application of laccases as a reported system in Aspergillus nidulans, Aspergillus niger and Trichoderma reesei. Monitoring of heterologously expressed laccase activity is done in colorometric enzyme assays and on agar plates with 2,2'-azino-di-(3-ethylbenzthiazolinsulfonate) (ABTS) as a substrate.

Subjects

Subjects :
Aspergillus -- Research
Fungi -- Research
Bacterial genetics -- Research
Biological sciences

Details

Language :
English
ISSN :
00992240
Volume :
72
Issue :
7
Database :
Gale General OneFile
Journal :
Applied and Environmental Microbiology
Publication Type :
Academic Journal
Accession number :
edsgcl.150417878

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