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Your search keyword '"Shivaprasad Dp"' showing total 2 results
Start Over Author "Shivaprasad Dp" Topic biofilms
2 results on '"Shivaprasad Dp"'

1. Phenotypic and genotypic characterization of biofilm forming, antimicrobial resistant, pathogenic Escherichia coli isolated from Indian dairy and meat products

Author

Pankaj Taneja, Divya Sachdev, Dinesh Kumar Bhardwaj, Sarita Gupta, Neetu Kumra Taneja, Praveen Patel, Madhusudana Girija Sanal, Ankita Singh Chakotiya, and Shivaprasad Dp

Subjects
Food Safety, Genotype, medicine.drug_class, Antibiotics, India, Biology, medicine.disease_cause, Microbiology, Foodborne Diseases, 03 medical and health sciences, Pathogenic Escherichia coli, Escherichia coli, medicine, Animals, Humans, 030304 developmental biology, 0303 health sciences, Virulence, 030306 microbiology, Biofilm, Quorum Sensing, General Medicine, biochemical phenomena, metabolism, and nutrition, Antimicrobial, biology.organism_classification, Anti-Bacterial Agents, Meat Products, Bacterial adhesin, Quorum sensing, Phenotype, Biofilms, Dairy Products, Bacteria, Food Science
Abstract

Escherichia coli are commensal gastrointestinal microflora of humans, but few strains may cause food-borne diseases. Present study aimed to identify antimicrobial resistant (AMR), biofilm-forming E. coli from Indian dairy and meat products. A total of 32 E. coli isolates were identified and evaluated for biofilm-formation. EMC17, an E. coli isolate was established as a powerful biofilm-former that attained maximum biofilm-formation within 96 h on glass and stainless-steel surfaces. Presence and expression of virulence-associated genes (adhesins, invasins and polysaccharides) and ability to adhere and invade human liver carcinoma HepG2 cell lines implicates EMC17 to be pathotype belonging to Extra-intestinal Pathogenic E. coli (ExPEC). Antibiotic profiling of EMC17 identified it as multi-drug resistant (MDR) strain, possessing extended spectrum β-lactamases (ESBL's) and biofilm phenotype. Early production of quorum sensing molecules (AHLs) alongside EPS production facilitated early onset of biofilm formation by EMC17. Furthermore, the biofilm-forming genes of EMC17 were significantly upregulated 3–27 folds in the biofilm-state. This study showed prevalence of MDR, biofilm-forming, pathogenic E. coli in Indian dairy and meat products that potentially serve as reservoirs for transmission of antimicrobial-resistant (AMR) genes of bacteria from food to humans and pose serious food safety threat.

Published
2021
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2. In vitro and in situ abrogation of biofilm formation in E. coli by vitamin C through ROS generation, disruption of quorum sensing and exopolysaccharide production.

Author

Shivaprasad, DP, Taneja, Neetu Kumra, Lakra, Anupama, and Sachdev, Divya

Subjects
*QUORUM sensing, *REACTIVE oxygen species, *BIOFILMS, *VITAMIN C, *BACTERIAL cells, *ANTIBACTERIAL agents
Abstract

• Pro-oxidant concentrations of VitC (125 mM) exhibited anti-microbial & anti-biofilm activity. • VitC impairs metabolic activity, QS & EPS production in E. coli biofilms. • VitC mediates ROS generation inducing protein & sugar leakage & causing cell death. • VitC-treated bacterial cells effectively downregulated biofilm forming genes. • In situ reduction of E. coli viability via VitC coating of paneer supported enhanced shelf-life. Emergence of antimicrobial drug-resistance amongst food-borne pathogens has led to severe deficit of available therapeutics and requires novel interventions. This study determined the activity of vitamin C (VitC), a natural antioxidant as powerful antibacterial agent against multidrug-resistant (MDR), biofilm-forming E. coli. Our findings revealed that VitC wield antibacterial action in dose-time dependent manner with minimum inhibitory concentration (MIC) of 125 mM. At these concentrations VitC impaired quorum sensing (QS) and exopolysaccharide (EPS) production and induced sugar and protein leakage from the bacterial cells by virtue of reactive oxygen species (ROS) generation. Furthermore, VitC-treated bacteria showed downregulation of genes underpinning biofilm signaling (luxS) and regulation (bssR) by up to 27-folds. Finally, this study demonstrated the promising antimicrobial application of VitC, in situ, in Indian soft cheese (paneer) when applied as a coating. Therefore, VitC can be applied as natural and safe 'antimicrobial' against biofilm-forming bacteria in food systems vis-à-vis other conventional antimicrobials. [ABSTRACT FROM AUTHOR]

Published
2021
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