1. Detailed characterization of an IncFII plasmid carrying blaOXA-48 from Lebanon.
- Author
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Moussa J, Panossian B, Nassour E, Salloum T, Abboud E, and Tokajian S
- Subjects
- Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Escherichia coli genetics, Lebanon, Microbial Sensitivity Tests, Plasmids genetics, Carbapenem-Resistant Enterobacteriaceae, beta-Lactamases genetics
- Abstract
Background: The spread of carbapenem-resistant Enterobacteriaceae is an important challenge and an increasing healthcare problem. OXA-48 is a class D carbapenemase that is usually localized on a conjugative plasmid belonging to the IncL incompatibility group., Methods: In this study, we used a combination of short- and long-read WGS approaches and molecular typing techniques to characterize the genetic environment of the smallest reported 27 029 bp IncFII plasmid carrying blaOXA-48 (pLAU-OXA48)., Results: The plasmid recovered from a clinical Escherichia coli isolate was positive for blaOXA-48, which was located within the Tn6237 composite transposon. Primers targeting junctions between the IncF fragment and Tn6237 for the rapid identification of pLAU-OXA48-like plasmids were designed., Conclusions: To our knowledge, this is the first report showing the complete sequence of an IncFII plasmid carrying blaOXA-48 within Tn6237 using hybrid assembly of long- and short-read sequencing., (© The Author(s) 2020. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For permissions, please email: journals.permissions@oup.com.)
- Published
- 2020
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