Genomic attributes of extended-spectrum β-lactamase-producing Escherichia coli isolated from patients in Lebanon.

Authors :: Tokajian S
Salloum T
Eisen JA
Jospin G
Farra A
Mokhbat JE
Coil DA
Source :: Future microbiology [Future Microbiol] 2017 Mar; Vol. 12, pp. 213-226. Date of Electronic Publication: 2017 Feb 27.
Publication Year :: 2017
Abstract: Aim: Extended-spectrum β-lactamase-producing (ESBL) Escherichia coli are a public threat worldwide. This study aimed at analyzing the genomic and functional attributes of nine ESBLs taken from rectal swabs.<br />Materials & Methods: Samples were isolated from patients admitted for gastrointestinal and urological procedures at the University Medical Center-Rizk Hospital (UMCRH) in Lebanon. Illumina paired-end libraries were prepared and sequenced.<br />Results: The isolates were distributed into five lineages: ST131, ST648, ST405, ST73 and ST38, and harbored bla <subscript>OXA-1</subscript> , bla <subscript>TEM-1B</subscript> , bla <subscript>TEM-1C</subscript> and aac(6')Ib-cr. ST131 isolates were carriers of stx2 converting I phage.<br />Conclusion: This is the first comprehensive genomic analysis performed on ESBLs in Lebanon.

Subjects :: Anti-Bacterial Agents pharmacology
Electrophoresis, Gel, Pulsed-Field
Escherichia coli classification
Escherichia coli drug effects
Escherichia coli enzymology
Genome, Bacterial
Genomics
Humans
Lebanon
Microbial Sensitivity Tests
Phylogeny
beta-Lactamases metabolism
Escherichia coli genetics
Escherichia coli Infections microbiology
beta-Lactamases genetics

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