1. THE PROMISE ON PHAGE DISPLAY: Customized Affinity and Specificity.
- Author
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Weiss, Gregory A. and Penner, Reginald M.
- Subjects
- *
BACTERIOPHAGES , *BIOSENSORS , *POLYPEPTIDES , *CHEMICAL affinity , *TRANSDUCERS , *ANTIGEN-antibody reactions , *PROSTATE-specific membrane antigen , *BIOMARKERS , *IMMUNOTECHNOLOGY - Abstract
The article discusses phage display mechanisms and phage-based biosensors. Topics include the: advantages of using the bacteriophage called filamentous phage; manipulation of polypeptides on virus particle surfaces; selection of a polypeptide receptor and affinity screening; methods for immobilizing filamentous phage on biosensor surfaces; antibody binding on a covalent virus surface; prostate-specific membrane antigen--a cancer biomarker; biosensor transducer--electrochemical impedance spectroscopy; and binding of target molecules to macroscopic gold electrodes.
- Published
- 2008
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