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Abstract: Objective: Hot water extract of adzuki beans (Vigna angularis) was subjected to HP-20 resin chromatography. The fraction eluted from the column using 40% ethanol (EtEx.40) was investigated by its effect on serum lipids in rats fed a high-fat cholesterol and/or cholesterol-free high-fat diet. Methods: The rats were divided into 4 groups. Groups 1 and 2 were fed a high-fat cholesterol diet with or without 3.5% EtEx.40 for 2 wk. Group 3s and 4 were fed a high-fat cholesterol-free diet with or without 3.5% EtEx.40 for 2 wk. Results: In the high-fat-cholesterol diet groups, there was no significant difference in food intake in the experimental diet group when compared with the control group. Serum total cholesterol level was significantly decreased in the rats fed the EtEx.40 diet, but there was no difference in fecal excretion of cholesterol and bile acid between the two dietary groups. Conversely, in the high-fat cholesterol-free diet groups, ingestion of EtEx.40 reduced serum triacylglycerol concentration. Conclusion: Ingestion of EtEx.40 suppressed serum cholesterol level in rats fed the high-fat cholesterol and serum triacylglycerol level in rats fed the high-fat cholesterol-free diet. These mechanisms did not become clear in this experiment. [Copyright &y& Elsevier]

Abstract: Objective: Recently, we reported that 40% ethanol fraction of hot-water extracts of adzuki (Vigna angularis; EtEx.40) suppressed the postprandial blood glucose level and serum insulin level in normal mice and streptozotocin-induced type 1 diabetic rats. The present study examined the hypoglycemic effect of EtEx.40 on blood glucose, insulin concentrations, organ weight, serum composition, and hepatic lipid content in spontaneously diabetic KK-Ay/Ta Jcl mice, a model for type 2 diabetes. Methods: To investigate the prevention of type 2 diabetes by EtEx.40 ingestion, 4-wk-old non-diabetic KK-Ay mice were fed an AIN-76 diet containing 5000 mg of EtEx.40/kg of body weight per day (EtEx.40) or an AIN-76 diet without EtEx.40 for 8 wk. Furthermore, to investigate the improvement of type 2 diabetes, 7-wk-old diabetic KK-Ay mice were fed EtEx.40 for 4 wk. Results: Compared with the control group, EtEx.40 supplementation had a significant effect in lowering blood glucose levels, water intake, serum insulin levels, urinary glucose, urinary microalbumin/creatinine ratio, liver triacylglycerol, and total cholesterol levels. Similar results were observed in 7-wk-old diabetic KK-Ay mice fed EtEx.40 for 4 wk. These effects were also found after short-term administration of EtEx40. Overall, EtEx.40 improved several diabetic symptoms in KK-Ay mice. Conclusion: EtEx.40 obtained from hot-water adzuki extracts showed preventive and ameliorative effects on the progression of diabetes in genetically diabetic KK-Ay mice. In the present study, we conclude that the preventive and ameliorative effects by EtEx.40 were due to the modulation of blood glucose levels and the protective effect against oxidative damage in diabetes mellitus. [Copyright &y& Elsevier]

Investigates the stimulation of melanogenesis by the hot water extract of adzuki beans (Vigna angularis) in cultured murine B16 melanoma cells and C3H mice. Indication that the distilled water fraction (WEx) can stimulate tyrosinase activity in cultured mouse B16 melanoma cells and hair color pigmentation in C3H mice; WEx stimulation of melanogenesis without inhibiting cell growth; Finding that WEx have pigmentation activity on hair color in C3H mice.

Examines the antimetastatic efficacy of hot-water extract from adzuki beans (EtEx.40) on experimental lung metastasis of murine B16 melanoma in mice. Cell proliferation; Assay for tumor invasion and migration; Cell adhesion assay; Gelatin zymography; Measurement of 1,1-diphenyl-2-picrylhydrazyl radical-scavenging activity.

Reports that a hot water extract obtained by boiling adzuki beans, Vigna angularis, to produce bean paste for Japanese cake showed inhibitory activity against alpha-glucosidase, alpha-amylase, maltase, sucrase, and isomaltase after HP-20 column chromatography. Preparation of the test materials; Oral glucose tolerance test on normal mice; Oral glucose tolerance test on rats with streptozotocin-induced diabetes.