1. Generation of Fel d 1 chain 2 genome-edited cats by CRISPR-Cas9 system.
- Author
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Lee SR, Lee KL, Song SH, Joo MD, Lee SH, Kang JS, Kang SM, Idrees M, Kim JW, and Kong IK
- Subjects
- Cats, Animals, Humans, CRISPR-Cas Systems, Allergens analysis, Enzyme-Linked Immunosorbent Assay, Hypersensitivity complications, Asthma etiology
- Abstract
Allergens from domestic cats (Felis catus) cause allergy-related health problems worldwide. Fel d 1 is a major allergen that causes severe allergic reactions in humans, including rhinitis, conjunctivitis, and life-threatening asthma. Therefore, patients with cat allergies anticipate hypoallergenic cats. We successfully generated Fel d 1 chain 2 (CH2) genome-edited cats using the CRISPR-Cas9 system in this study. T7 endonuclease 1 assay and Sanger sequencing were used to confirm the mutation in CH2 genome-edited cats. Fel d 1 level in CH2 genome-edited cats were assessed by enzyme-linked immunosorbent assay (ELISA). Remarkably, ELISA showed that the level of Fel d 1 in the CH2 homozygous genome-edited cat (Name: Alsik) was extremely low compared with that in wild type domestic cats and could be hypoallergenic cats. Additionally, we successfully cloned the CH2 homozygous genome-edited cat using cytoplasm injection clone technology. The cloned CH2 homozygous genome-edited cat was verified using microsatellite analysis. Creating hypoallergenic cats using the CRISPR-Cas9 system is a significant step forward because these cats can safely approach allergic patients., (© 2024. The Author(s).)
- Published
- 2024
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