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Blockade of IL-33/ST2 ameliorates airway inflammation in a murine model of allergic asthma.
- Source :
-
Experimental lung research [Exp Lung Res] 2014 Mar; Vol. 40 (2), pp. 66-76. Date of Electronic Publication: 2014 Jan 21. - Publication Year :
- 2014
-
Abstract
- Objective: Interleukin (IL)-33 is involved in the development of lung inflammation by inducing or amplifying Th2 type-mediated responses in various animal models of allergic asthma. The ST2 gene is a member of the IL-1 receptor family, producing a transmembrane form (ST2L) and a soluble secreted form (sST2). sST2 has been shown to block this IL-33/ST2 signaling pathway. This study aimed to investigate whether anti-IL-33 and sST2 reduced airway inflammation in a murine model of asthma.<br />Methods: BALB/c mice were sensitized and challenged with ovalbumin (OVA), and the effect of sST2 and anti-IL-33 antibody on airway inflammation and airway hyperresponsiveness (AHR) was evaluated. Furthermore, we measured changes in various cytokines in the bronchoalveolar lavage (BAL) fluid when treated with sST2 or anti-IL-33.<br />Results: We observed that anti-IL-33 antibody and sST2 exert a negative regulation on OVA-mediated allergic airway inflammation. Both treatments reduced total cell counts and eosinophil counts in BAL fluid and AHR to methacholine. The Th2 cytokines, such as IL-4, IL-5, and IL-13 in BAL fluid were also significantly decreased after both treatments. However, there were no changes in the level of TGF- ß1 and IL-10 after each treatment.<br />Conclusions: These results suggest that anti-IL-33 as well as sST2 have therapeutic potential for allergic asthma through inhibition of Th2 cytokine production.
- Subjects :
- Animals
Antibodies, Anti-Idiotypic pharmacology
Asthma metabolism
Bronchoalveolar Lavage Fluid
Cytokines metabolism
Disease Models, Animal
Female
Immunoglobulin E blood
Immunoglobulin G blood
Interleukin-1 Receptor-Like 1 Protein
Interleukin-33
Interleukins metabolism
Mice
Mice, Inbred BALB C
Pneumonia metabolism
Pneumonia pathology
Receptors, Interleukin metabolism
Signal Transduction drug effects
Signal Transduction physiology
Th2 Cells drug effects
Th2 Cells metabolism
Th2 Cells pathology
Antibodies, Anti-Idiotypic therapeutic use
Asthma chemically induced
Asthma complications
Interleukins antagonists & inhibitors
Ovalbumin adverse effects
Pneumonia prevention & control
Receptors, Interleukin antagonists & inhibitors
Subjects
Details
- Language :
- English
- ISSN :
- 1521-0499
- Volume :
- 40
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Experimental lung research
- Publication Type :
- Academic Journal
- Accession number :
- 24446582
- Full Text :
- https://doi.org/10.3109/01902148.2013.870261