PURPOSE: To design, construct, and evaluate quantitative MR phantoms that mimic MRI signals from the liver with simultaneous control of three parameters: proton-density fat-fraction (PDFF), [Formula: see text] , and T(1). These parameters are established biomarkers of hepatic steatosis, iron overload, and fibrosis/inflammation, respectively, which can occur simultaneously in the liver. METHODS: Phantoms including multiple vials were constructed. Peanut oil was used to modulate PDFF, MnCl(2) and iron microspheres were used to modulate [Formula: see text] , and NiCl(2) was used to modulate the T(1) of water (T(1,water)). Phantoms were evaluated at both 1.5T and 3.0T using stimulated echo acquisition mode MR spectroscopy (STEAM-MRS) and chemical shift encoded (CSE) MRI. STEAM-MRS data were processed to estimate T(1,water), T(1,fat), [Formula: see text] , and [Formula: see text] for each vial. CSE-MRI data were processed to generate PDFF and [Formula: see text] maps, and measurements were obtained in each vial. Measurements were evaluated using linear regression and Bland-Altman analysis. RESULTS: High quality PDFF and [Formula: see text] maps were obtained with homogeneous values throughout each vial. High correlation was observed between imaging PDFF with target PDFF (slope=0.94–0.97, R(2)=0.994–0.997) and imaging [Formula: see text] with target [Formula: see text] (slope=0.84–0.88, R(2)=0.935–0.943) at both 1.5T and 3.0T. [Formula: see text] and [Formula: see text] were highly correlated with slope close to 1.0 at both 1.5T (slope=0.90, R(2)=0.988) and 3.0T (slope=0.99, R(2)=0.959), similar to the behavior observed in vivo. T(1,water) (500–1200ms) was controlled with varying NiCl(2) concentration, while T(1,fat) (300ms) was independent of NiCl(2) concentration. CONCLUSION: Novel quantitative MRI phantoms that mimic the simultaneous presence of fat, iron, and fibrosis in the liver were successfully developed and validated. KEYWORDS: Quantitative imaging biomarkers, phantom, proton-density fat-fraction, [Formula: see text] , T(1), liver