1. The plant streptolysin S (SLS)-associated gene B confers nitroaromatic tolerance and detoxification
- Author
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Rujia Chen, Yue Lu, Enying Zhang, Zhiyang Chen, Liexiang Huangfu, Zhihao Zuo, Yu Zhao, Minyan Zhu, Zihui Zhang, Mingli Chuan, Qing Bu, Qianfeng Huang, Hanyao Wang, Yang Xu, Pengcheng Li, Youli Yao, Yong Zhou, Chenwu Xu, and Zefeng Yang
- Subjects
Biodegradation, Environmental ,Environmental Engineering ,Bacterial Proteins ,Health, Toxicology and Mutagenesis ,Streptolysins ,Arabidopsis ,Soil Pollutants ,Environmental Chemistry ,Nitroreductases ,Plants ,Pollution ,Waste Management and Disposal ,Trinitrotoluene - Abstract
Nitroaromatic compounds, as the important chemical feedstock, have caused widespread environmental contaminations, and exhibited high toxicity and mutagenic activity to nearly all living organisms. The clean-up of nitroaromatic-contaminated soil and water has long been a major international concern. Here, we uncovered the role of a novel nitroreductase family gene, streptolysin S (SLS)-associated gene B (SagB), in enhancing nitroaromatic tolerance and detoxification of plants, and its potential application in phytoremediation of nitroaromatic contaminations. The expression of both the Arabidopsis and rice SagB genes is significantly induced by multiple hazardous nitroaromatic substances, including explosive pollutant 2,4,6-trinitrotoluene (TNT), natural compound 1-nitropyrene (1-NP) and herbicide pendimethalin (Pen). In vitro and in vivo evidences revealed that plant SagBs possess activities in degradation of these nitroaromatic substances. Arabidopsis and rice transgenic assays suggested that plant SagB genes increase tolerance and detoxification of nitroaromatic through facilitating its transformation to the amino derivative. More importantly, overexpression of plant SagBs increase their ability in TNT uptake, and remove more TNT from the growth culture. Our findings shed novel insights into a plant endogenous nitroreductase-mediated nitroaromatic tolerance and detoxification, and provide a new gene target for phytoremediation of nitroaromatic-contaminated environments.
- Published
- 2022