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Cloning and characterization of Arabidopsis homologues of the animal CstF complex that regulates 3' mRNA cleavage and polyadenylation.
- Source :
-
Journal of Experimental Botany . Nov2002, Vol. 53 Issue 378, p2277-2278. 2p. 2 Diagrams. - Publication Year :
- 2002
-
Abstract
- The 3' cleavage and polyadenylation of mRNAs has been studied in detail in animals and yeast, but not in plants. Aimed at elucidating the regulation of mRNA 3' end formation in plants, three Arabidopsis cDNAs encoding homologues of the animal proteins CstF-64, CstF-77 and CstF-50 that form the cleavage stimulating factor of the polyadenylation machinery have been cloned. It is shown experimentally that the N-terminal domain of the Arabidopsis CstF-64 homologue binds the mRNA 3' noncoding region in an analogous manner to the animal protein. It is also shown that the Arabidopsis CstF-64 and CstF-77 homologues strongly interact with each other in a similar way to their animal counterparts. These results imply that these Arabidopsis homologues belong to the polyadenylation machinery of nuclear mRNAs. [ABSTRACT FROM AUTHOR]
- Subjects :
- *MESSENGER RNA
*ANIMALS
*YEAST
*PLANTS
*ARABIDOPSIS
Subjects
Details
- Language :
- English
- ISSN :
- 00220957
- Volume :
- 53
- Issue :
- 378
- Database :
- Academic Search Index
- Journal :
- Journal of Experimental Botany
- Publication Type :
- Academic Journal
- Accession number :
- 45264737
- Full Text :
- https://doi.org/10.1093/jxb/erf073