1. CK2-mediated phosphorylation of Che-1/AATF is required for its pro-proliferative activity.
- Author
-
Catena V, Bruno T, Iezzi S, Matteoni S, Salis A, Sorino C, Damonte G, and Fanciulli M
- Subjects
- Cell Proliferation physiology, Humans, Phosphorylation, Transfection, Apoptosis Regulatory Proteins metabolism, Casein Kinase II metabolism, Repressor Proteins metabolism
- Abstract
Background: Che-1/AATF (Che-1) is an RNA polymerase II binding protein involved in several cellular processes, including proliferation, apoptosis and response to stress. We have recently demonstrated that Che-1 is able to promote cell proliferation by sustaining global histone acetylation in multiple myeloma (MM) cells where it interacts with histone proteins and competes with HDAC class I members for binding., Methods: Site-directed Mutagenesis was performed to generate a Che-1 mutant (Che-1 3S) lacking three serine residues (Ser
316 , Ser320 and Ser321 ) in 308-325 aa region. Western blot experiments were conducted to examine the effect of depletion or over-expression of Che-1 and Che-1 3S mutant on histone acetylation, in different human cancer cell lines. Proliferation assays were assessed to estimate the change in cells number when Che-1 was over-expressed or deleted. Immunoprecipitation assays were performed to evaluate Che-1/histone H3 interaction when Ser316 , Ser320 and Ser321 were removed. The involvement of CK2 kinase in Che-1 phosphorylation at these residues was analysed by in vitro kinase, 2D gel electrophoresis assays and mass spectrometry analysis., Results: Here, we confirmed that Che-1 depletion reduces cell proliferation with a concomitant general histone deacetylation in several tumor cell lines. Furthermore, we provided evidence that CK2 protein kinase phosphorylates Che-1 at Ser316 , Ser320 and Ser321 and that these modifications are required for Che-1/histone H3 binding. These results improve our understanding onto the mechanisms by which Che-1 regulates histone acetylation and cell proliferation., Conclusions: Che-1 phosphorylation at Ser316 , Ser320 and Ser321 by CK2 promotes the interaction with histone H3 and represents an essential requirement for Che-1 pro-proliferative ability., (© 2021. The Author(s).)- Published
- 2021
- Full Text
- View/download PDF