1. Dental Epithelial Histomorphogenesis in vitro
- Author
-
Bing Hu, Hervé Lesot, Fabienne Perrin-Schmitt, S. Bopp-Kuchler, Amal Nadiri, Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I, and Toussaint, Jean-Luc
- Subjects
0301 basic medicine ,Pathology ,Cellular differentiation ,Apoptosis ,Cell Communication ,Histogenesis ,[SDV.BBM.BM] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,MESH: Enamel Organ ,Epithelium ,Mesoderm ,Tissue Culture Techniques ,Mice ,0302 clinical medicine ,Tissue engineering ,Image Processing, Computer-Assisted ,Morphogenesis ,MESH: Animals ,Mice, Inbred ICR ,MESH: Mesoderm ,Cell Differentiation ,MESH: Image Processing, Computer-Assisted ,Enamel knot ,medicine.anatomical_structure ,MESH: Epithelial Cells ,MESH: Cell Differentiation ,medicine.medical_specialty ,Mesenchyme ,MESH: Tooth Germ ,MESH: Imaging, Three-Dimensional ,Biology ,03 medical and health sciences ,Imaging, Three-Dimensional ,stomatognathic system ,MESH: Cell Communication ,medicine ,Animals ,MESH: Tissue Culture Techniques ,MESH: Mice, Inbred ICR ,General Dentistry ,MESH: Mice ,MESH: Apoptosis ,Enamel Organ ,Enamel organ ,Tooth Germ ,Epithelial Cells ,[SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology ,030206 dentistry ,MESH: Morphogenesis ,stomatognathic diseases ,MESH: Epithelium ,030104 developmental biology - Abstract
International audience; Recent developments in tooth-tissue engineering require that we understand the regulatory processes to be preserved to achieve histomorphogenesis and cell differentiation, especially for enamel tissue engineering. Using mouse first lower molars, our objectives were: (1) to determine whether the cap-stage dental mesenchyme can control dental epithelial histogenesis, (2) to test the role of the primary enamel knot (PEK) in specifying the potentialities of the dental mesenchyme, and (3) to evaluate the importance of positional information in epithelial cells. After tissue dissociation, the dental epithelium was further dissociated into individual cells, re-associated with dental mesenchyme, and cultured. Epithelial cells showed a high plasticity: Despite a complete loss of positional information, they rapidly underwent typical dental epithelial histogenesis. This was stimulated by the mesenchyme. Experiments performed at E13 demonstrated that the initial potentialities of the mesenchyme are not specified by the PEK. Positional information of dental epithelial cells does not require the memorization of their history.
- Published
- 2005