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Copyright of Journal of Practical Medicine / Shiyong Yixue Zazhi is the property of Journal of Practical Medicine Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of China Medical University is the property of Journal of China Medical University Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To investigate the mechanism of peroxiredoxin 2 (PRDX2)in ethanol-induced reproductive damage in male mice. Methods: Male Kunming mice were divided into control group and model group. The control group was treated with distilled water and the model group was treated with ethanol for 12 weeks. Serum was collected for hormone level determination. Sperm was collected, one part was used for semen analysis, the other part was used for Q-PCR detecting the mRNA expressions of PRDX2, BCL-2, BAX and Caspase3, Western blot detecting the protein expressions of PRDX2, BCL-2, BAX, Caspase3 and Cleaved-Caspase3 and Immunofluorescence staining identifying the expression of PRDX2. Statistical analysis results. Results: Compared with the control group, the sperm motility and the androgen level of the model group decreased, the estrogen level increased(P<0.05). Compared with the control group, the PRDX2 and BCL-2 protein and mRNA levels of the model group were significantly decreased (P<0.05). Immunofluorescence showed that PRDX2 could be expressed in sperm, and the fluorescence intensity of sperm in model group was significantly reduced. BAX and Caspase3 mRNA of the model group were significantly higher than that of the control group (P<0.05). The BAX and Cleaved-Caspase3 protein levels of the model group were significantly increased (P<0.05). Pearson correlation coefficient analysis showed that PRDX2 was positively correlated with BCl-2 and negatively correlated with BAX and Caspase3 (P<0.05). Conclusion: Ethanol causes the decrease of sperm PRDX2 expression, indirectly induces sperm apoptosis, affects sperm production and development, and impairs the reproductive function of male mice. [ABSTRACT FROM AUTHOR]

BACKGROUND: Magnesium ions can reduce the sensitivity of vascular endothelial cells to adverse stimuli and regulate vascular endothelial function by scavenging free radicals, stabilizing cell membranes and inhibiting inflammation. However, the effect of magnesium ions on endothelial progenitor cells is still unclear. OBJECTIVE: To explore the effects of magnesium on the proliferation, adhesion and apoptosis of endothelial progenitor cells in patients with preeclampsia. METHODS: From March 2016 to October 2018, 17 preeclampsia pregnant patients and 15 normal pregnant women who were admitted in the Obstetrics Department of Hebei General Hospital were enrolled. Endothelial progenitor cells were isolated and cultured from umbilical cord blood in the two groups of pregnant women. Endothelial progenitor cells isolated from each preeclampsia pregnant patient were divided into four groups: preeclampsia group was intervened with PBS, and low, middle and high concentration magnesium ion groups were intervened with the medium containing 4, 8, and 16 mmol/L magnesium ions, respectively. Endothelial progenitor cells from normal pregnant women were treated with PBS as control group. After 24 hours of intervention, cell proliferation, adhesion, apoptosis, inflammation (interleukin-6, interleukin-8, tumor necrosis factor-α, monocyte chemoattractant protein-1 levels) and oxidative stress (superoxide dismutase and malondialdehyde levels) were detected. The study protocol was approved by the Ethics Committee of Hebei General Hospital with the approval No. 2013linshen13. RESULTS AND CONCLUSION: (1) Compared with the control group, the cell proliferation and adhesion of the preeclampsia group were significantly decreased (P < 0.05); the apoptotic rate significantly increased (P < 0.05); the expression of Bax and Active Caspase-3 increased, and the expression of Bcl-2 decreased (both P < 0.05); the levels of interleukin-6, interleukin-8, tumor necrosis factor-α, monocyte chemoattractant protein-1, and malondialdehyde increased (P < 0.05), and the activity of superoxide dismutase decreased (P < 0.05). (2) Compared with the preeclampsia group, the cell proliferation and adhesion of the magnesium ion intervention groups significantly increased (P < 0.05); the apoptotic rate decreased (P < 0.05); the expression of Bax and Active Caspase-3 decreased, and the expression of Bcl-2 increased (P < 0.05); the levels of interleukin-6, interleukin-8, tumor necrosis factor-α, monocyte chemoattractant protein-1, malondialdehyde decreased (P < 0.05), and the level of superoxide dismutase increased (P < 0.05). And the improvement in the high concentration magnesium ion group was better than that in the low and middle concentration magnesium ion groups (P < 0.05). To conclude, magnesium ions can promote the proliferation and adhesion of endothelial progenitor cells in vitro, inhibit the secretion of inflammatory factors, and reduce the apoptotic rate and oxidative stress level in patients with preeclampsia. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Kunming Medical University / Kunming Yike Daxue Xuebao is the property of Kunming Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To investigate the effects of miRNA-219-5p on the proliferation， apoptosis， and invasion of pancreatic carcinoma cells and its mechanism. Methods Real-time fluorescence quantitative PCR (qPCR) was carried out to determine miR-219-5p expression in the pancreatic carcinoma cell lines Panc-1，AsPC-1，and Bxpc-3 and normal pancreatic ductal epithelial cell line HPDE6-C7. Further, Panc-1 cells were chosen and were divided into two groups： the normal control group (NC group) (transfection with miR-219-5p scramble) and miR-219-5p group (transfection with miR- 219-5p mimics). The cell proliferation and apoptosis were detected by MTT assay and flow cytometry. The invasion ability was checked by transwell assay. The expression of epidermal growth factor receptor (EGFR) and Cleaved Caspase-3 proteins was examined by Western blotting. Results MiR-219-5p expression was lower in pancreatic carcinoma cell lines Panc-1，AsPC-1，and Bxpc-3，as compared with that in the pancreatic ductal epithelial cell line HPDE6-C7 (all P < 0. 01). The 0D490nm of the miR-219-5p group was significantly lower than that of the NC group at 72，96，and 120 h ( all P <0.01)； the apoptosis rate was higher (all P <0. 01). The invasive cells were (75. 3 ± 6. 9)/Hp in the miR-219-5p group，which was significantly lower than that [(172.5 ±10.5)/Hp] in the NC group (P <0.01 ). The expressive level ofEGFRinthemiR-219-5pgroupwassignificantlylowerthanNCgroup(0.31±0.09vs.1.0，P<0.01). The expression of Cleaved Caspase-3 was up-regulated in the miR-219-5p group as compared with that of NC group (2.7 ±0. 15 vs. 1.0, P <0.01). Conclusions The miR-219-5p is lowly expressed in pancreatic carcinoma cell lines. The over-expression of miR-219-5p can inhibit the proliferation and invasion of pancreatic carcinoma cells, and induce the apoptosis, possibly though the down-regulated protein expression of EGFR and up-regulated protein expression of Cleaved Caspase-3. [ABSTRACT FROM AUTHOR]

Objective To study the influence of artesunate (Akt) on the proliferation and apoptosis of human head and neck squamous cell carcinoma (HNSCC), and to explore its molecular mechanism thereof. Methods The HNSCC cell line, UM-SCC-10A cells, was cultured in vitro. The Inhibitory concentration 50 (IC50) was examined by MTT assay. The cell morphological changes were observed under inverted light micro-scope after being interfered by 0, 2.5, 5, 10, 20 and 40μmol/L Akt. Cell cycle changes and apoptosis were measured by flow cytometry. And the expression of cell cycle regulators and apoptotic associated protein were detected by Western blot assay. Results MTT assay demonstrated that Akt significantly inhibited the proliferation of UM-SCC-10A cells in dose-dependent manner. After UM-SCC-10A cells were treated with Akt for 48 h, IC50 was 15.01μmol/L. Morphological changes of cell apoptosis such as karyopyknosis and conglomeration were ob?served by Hoechst 33258 staining. Flow cytometry showed that the apoptosis was associated with cell cycle arrest during the G1 phase. Western blot analysis showed that p53 and p21 protein was up-regulated and Cyclin D protein was down-regulated. Furthermore, results revealed that Bcl-2 associated X protein induced by a mitochondrial pathway, cytochrome C and caspase-3 were up-regulated, and Bcl-2 and procaspase-3 were down-regulated. The mitochondrial membrane potential was reduced. Conclusion Artesunate can induce apoptosis of UM-SCC-10A cells via a mitochondrial pathway, which was associated with cell cycle arrest in the G1 phase. As a result, artesunate has an obvious inhibitory effects on proliferation of UM-SCC-10A cells. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)