镁对子痫前期患者内皮祖细胞增殖、黏附和凋亡的影响.

BACKGROUND: Magnesium ions can reduce the sensitivity of vascular endothelial cells to adverse stimuli and regulate vascular endothelial function by scavenging free radicals, stabilizing cell membranes and inhibiting inflammation. However, the effect of magnesium ions on endothelial progenitor cells is still unclear. OBJECTIVE: To explore the effects of magnesium on the proliferation, adhesion and apoptosis of endothelial progenitor cells in patients with preeclampsia. METHODS: From March 2016 to October 2018, 17 preeclampsia pregnant patients and 15 normal pregnant women who were admitted in the Obstetrics Department of Hebei General Hospital were enrolled. Endothelial progenitor cells were isolated and cultured from umbilical cord blood in the two groups of pregnant women. Endothelial progenitor cells isolated from each preeclampsia pregnant patient were divided into four groups: preeclampsia group was intervened with PBS, and low, middle and high concentration magnesium ion groups were intervened with the medium containing 4, 8, and 16 mmol/L magnesium ions, respectively. Endothelial progenitor cells from normal pregnant women were treated with PBS as control group. After 24 hours of intervention, cell proliferation, adhesion, apoptosis, inflammation (interleukin-6, interleukin-8, tumor necrosis factor-α, monocyte chemoattractant protein-1 levels) and oxidative stress (superoxide dismutase and malondialdehyde levels) were detected. The study protocol was approved by the Ethics Committee of Hebei General Hospital with the approval No. 2013linshen13. RESULTS AND CONCLUSION: (1) Compared with the control group, the cell proliferation and adhesion of the preeclampsia group were significantly decreased (P < 0.05); the apoptotic rate significantly increased (P < 0.05); the expression of Bax and Active Caspase-3 increased, and the expression of Bcl-2 decreased (both P < 0.05); the levels of interleukin-6, interleukin-8, tumor necrosis factor-α, monocyte chemoattractant protein-1, and malondialdehyde increased (P < 0.05), and the activity of superoxide dismutase decreased (P < 0.05). (2) Compared with the preeclampsia group, the cell proliferation and adhesion of the magnesium ion intervention groups significantly increased (P < 0.05); the apoptotic rate decreased (P < 0.05); the expression of Bax and Active Caspase-3 decreased, and the expression of Bcl-2 increased (P < 0.05); the levels of interleukin-6, interleukin-8, tumor necrosis factor-α, monocyte chemoattractant protein-1, malondialdehyde decreased (P < 0.05), and the level of superoxide dismutase increased (P < 0.05). And the improvement in the high concentration magnesium ion group was better than that in the low and middle concentration magnesium ion groups (P < 0.05). To conclude, magnesium ions can promote the proliferation and adhesion of endothelial progenitor cells in vitro, inhibit the secretion of inflammatory factors, and reduce the apoptotic rate and oxidative stress level in patients with preeclampsia. [ABSTRACT FROM AUTHOR]