1. Reactive oxygen species production and antioxidant enzyme activity during epididymal sperm maturation in Corynorhinus mexicanus bats.
- Author
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Arenas-Ríos E, Rosado García A, Cortés-Barberena E, Königsberg M, Arteaga-Silva M, Rodríguez-Tobón A, Fuentes-Mascorro G, and León-Galván MA
- Subjects
- Animals, Chiroptera, Epididymis anatomy & histology, Gene Expression Regulation, Enzymologic physiology, Glutathione Peroxidase genetics, Male, Superoxide Dismutase genetics, Antioxidants metabolism, Epididymis physiology, Glutathione Peroxidase metabolism, Reactive Oxygen Species metabolism, Sperm Maturation physiology, Superoxide Dismutase metabolism
- Abstract
Prolonged sperm storage in the epididymis of Corynorhinus mexicanus bats after testicular regression has been associated with epididymal sperm maturation in the caudal region, although the precise factors linked with this phenomenon are unknown. The aim of this work is to determine the role of reactive oxygen species (ROS) and changes in antioxidant enzymatic activity occurring in the spermatozoa and epididymal fluid over time, in sperm maturation and storage in the caput, corpus and cauda of the bat epididymis. Our data showed that an increment in ROS production coincided with an increase in superoxide dismutase (SOD) activity in epididymal fluid and with a decrease in glutathione peroxidase (GPX) activity in the spermatozoa in at different time points and epididymal regions. The increase in ROS production was not associated with oxidative damage measured by lipid peroxidation. The results of the current study suggest the existence of a shift in the redox balance, which might be associated with sperm maturation and storage., (Copyright © 2016 Society for Biology of Reproduction & the Institute of Animal Reproduction and Food Research of Polish Academy of Sciences in Olsztyn. Published by Elsevier Urban & Partner Sp. z o.o. All rights reserved.)
- Published
- 2016
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