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Alcalase hydrolyzates were prepared from the albumin (AH) and globulin (GH) fractions of eight chickpea (Cicer arietinum L.) genotypes from Mexico and 10 from other countries. Protein content, antioxidant activity (AA) (ABTS, DPPH), and degree of hydrolysis were evaluated and the best genotype was selected by principal component analysis. The hydrolyzates of the chosen genotype were analyzed for its antidiabetic potential measured as inhibition of α-amylase, α-glucosidase, and dipeptidyl peptidase-4 (DPP4). Peptide profiles were obtained by liquid chromatography-mass spectrometry (UPLC-DAD-MS), and the most active peptides were analyzed by molecular docking. The average antioxidant activity of albumin hydrolyzates was higher than that of globulin hydrolyzates. ICC3761 was the selected genotype and peptides purified from the albumin hydrolyzate showed the best antioxidant activity and antidiabetic potential (FEI, FEL, FIE, FKN, FGKG, and MEE). FEI, FEL, and FIE were in the same chromatographic peak and this mixture showed the best ABTS scavenging (78.25%) and DPP4 inhibition (IC 50  = 4.20 µg/ml). MEE showed the best DPPH scavenging (47%). FGKG showed the best inhibition of α-amylase (54%) and α-glucosidase (56%) and may be a competitive inhibitor based on in silico-predicted interactions with catalytic amino acids in the active site of both enzymes. These peptides could be used as nutraceutical supplements against diseases related to oxidative stress and diabetes. PRACTICAL APPLICATION: This study showed that chickpea protein hydrolyzates are good sources of peptides with antidiabetic potential, showing high antioxidant activity and inhibition of enzymes related to carbohydrate metabolism and type 2 diabetes. These hydrolyzates could be formulated in functional foods for diabetes., (© 2021 Institute of Food Technologists®.)

Red arils of Pithecellobium dulce fruit, commonly known as guamuchil, show high antioxidant (AOx) and α-glucosidase inhibitory (IαG) activities, which have been mainly associated with the content of unknown anthocyanins. In this study, the AOx (i.e., DPPH and ABTS as Trolox equivalents, μmol TE/g) and IαG (as half-maximal inhibitory concentration, IC 50 , mg/mL) activities of the anthocyanin-rich fraction (ARF) obtained from red arils were contrasted with those of the methanol extract (ME), and the main ARF anthocyanins were characterized by HPLC-DAD-ESI-MS, GC-MS and 1 H-NMR. The AOx and IαG values of the ARF (DPPH = 597.8; ABTS = 884.01; IαG = 0.06) were better than those of the ME (DPPH = 41.5; ABTS = 142.3; IαG = 17.5); remarkably, the ARF IαG value was about 42 times lower than that of acarbose. The main anthocyanins in ARF were pelargonidin 3-O-glucoside and cyanidin 3-O-glucoside. Thus, the consumption of red P. dulce arils could provide health benefits for prevention/treatment of chronic degenerative diseases such as diabetes.

Chickpea (Cicer arietinum L.) genotypes, nine kabuli from Mexico and 9 desi from other countries, were investigated for their phenolic profiles and antioxidant activity (AA). Phenolics in methanol extracts (ME) were analyzed by ultra-performance liquid chromatography coupled to diode array detection and mass spectrometry (UPLC-DAD-MS), whereas the AA was measured as Trolox equivalents (TE) by ABTS, DPPH and FRAP methods. Twenty phenolic compounds were identified in the ME and their levels showed a great variability among the chickpea genotypes. Phenolic acids and flavonoids were the most abundant compounds in kabuli and desi genotypes, respectively. The AA values (μmol TE/ 100 g dw) by ABTS (278-2417), DPPH (52-1650), and FRAP (41-1181) were mainly associated with the content of sinapic acid hexoside, gallic acid, myricetin, quercetin, catechin, and isorhamnetin, suggesting they are the main compounds responsible for the AA. The sum of the AA obtained for standards of these compounds evaluated at the concentration found in the extracts accounted for 34.3, 69.8, and 47.0% of the AA in the extract by ABTS, DPPH, and FRAP, respectively. In the AA by DPPH, most of the mixtures of these compounds resulted in synergistic interactions. Three desi genotypes with black seeds (ICC 4418, ICC 6306, and ICC 3761) showed the highest AA and flavonoids content, whereas the most promising kabuli genotypes were Surutato 77, Bco. Sin. 92, and Blanoro that showed the highest values of phenolic acids. These genotypes represent good sources of antioxidants for the improvement of nutraceutical properties in chickpea.

Context: Helicteres vegae Cristóbal (Sterculiaceae) (Hv) and Heliopsis sinaloensis B.L. Turner (Asteraceae) (Hs) are endangered and poorly studied plant species; related plants have been used against chronic-degenerative and infectious diseases. Therefore, Hv and Hs could be sources of bioactive compounds against these illnesses., Objective: To determine the chemical composition and biological activities (antioxidant, antimutagenic and antimicrobial) of Hv and Hs leaves (L) and stems (S)., Materials and Methods: Methanol extracts (ME) of each plant/tissue were evaluated for their phytochemicals; phenolics (HPLC-DAD-ESI-MS); antioxidant activity (AA) (0.125-4 mg/mL) (DPPH, ABTS, ORAC and β-carotene discoloration); antimutagenicity (0.5 and 1 mg/plate) (Ames assay, tester strain Salmonella enterica serovar Typhimurium YG1024, 1-nitropyrene as mutagen); activity against human pathogens (1 mg/mL); and toxicity (0.01-2 mg/mL) (Artemia salina assay)., Results: All ME showed flavonoids and triterpenes/steroids. The ME-SHv had the highest content of total phenolics (TP) (2245.82 ± 21.45 mg GAE/100 g d.w.) and condensed tannins (603.71 ± 1.115 mg CE/100 g d.w.). The compounds identified were flavonoids (kaempferol 7-O-coumaroylhexoside, and two kaempferol 7-O-rhamnosylhexosides) and phenolics [rosmarinic acid, and 3'-O-(8″-Z-caffeoyl) rosmarinic acid]. The ME-LHs showed the highest content of flavonoids (357.88 mg RE/g d.w.) and phenolic acids (238.58 mg CAE/g d.w.) by HPLC. The ME-SHv showed the highest AA. All ME were strong antimutagens (63.3-85.7%). Only the Hs extracts were toxic (ME-LHs, LC 50  = 94.9 ± 1.7 μg/mL; ME-SHs, LC 50  = 89.03 ± 4.42 μg/mL)., Discussion and Conclusions: Both Hv and Hs are potential sources of preventive and therapeutic agents against chronic-degenerative diseases.

The phenolic content and antioxidant and antimutagenic activities from the peel and seeds of different tomato types (grape, cherry, bola and saladette type), and simulated tomato industrial byproducts, were studied. Methanolic extracts were used to quantify total phenolic content, groups of phenolic compounds, antioxidant activities, and the profile of phenolic compounds (by HPLC-DAD). Antimutagenic activity was determined by Salmonella typhimurium assay. The total phenolic content and antioxidant activity of tomato and tomato byproducts were comparable or superior to those previously reported for whole fruit and tomato pomace. Phenolic compounds with important biological activities, such as caffeic acid, ferulic acid, chlorogenic acids, quercetin-3-β-O-glycoside, and quercetin, were quantified. Differences in all phenolic determinations due to tomato type and part of the fruit analyzed were observed, peel from grape type showing the best results. Positive antimutagenic results were observed in all samples. All evaluated materials could be used as a source of potential nutraceutical compounds.

We report for the first time the antioxidant and antimutagenic activities of fractions from Randia echinocarpa fruit, which is a Rubiaceae plant native to Sinaloa, Mexico. This fruit has been traditionally used in the prevention or treatment of cancer, among other diseases. The pulp of the fruit was sequentially extracted with solvents of different polarity (i.e. hexane, chloroform, methanol and water). A high extraction yield was obtained with methanol (72.17% d.w.). The aqueous extract showed the highest content of phenolics (2.27 mg/g as ferulic acid equivalents) and the highest antioxidant activity based on the beta-carotene bleaching method (486.15). The commercial antioxidant BHT was used as control (835.05). Antimutagenic activity of the aqueous extract (0-500 microg/tube) was evaluated using the Salmonella microsuspension assay (YG1024 strain) and 1-NP as the mutagen (50 and 100 ng/tube). The aqueous extract was neither toxic nor mutagenic and the percentage of inhibition on 1-NP mutagenicity was 32 and 53% at doses of 50 and 100 ng/tube, respectively. The results of the double incubation assay suggest that the extract inhibited the mutagenicity of 1-NP by a combination of desmutagenic and bioantimutagenic effects.

Chickpea (ICC3761) protein hydrolysates have shown high in vitro antioxidant activity (AoxA) and antidiabetic potential. The aim of this study was to evaluate the in vivo activities (i.e., antioxidant, anti‐inflammatory, hypoglycemic, and anti‐hyperglycemic) of chickpea albumin hydrolysates (CAH) obtained with alcalase and pepsin‐pancreatin (fractions ≤ 10 kDa). The CAH were analyzed for degree of hydrolysis (DH), electrophoretic and chromatographic profiles, and in vitro AoxA (2,2′‐azino‐bis(3‐ethylbenzothiazolin)‐6‐sulfonic acid [ABTS], 2,2‐diphenyl‐1‐pycrilhydrazyl [DPPH]). They were also evaluated for AoxA, anti‐inflammatory and hypo‐ and anti‐hyperglycemic activities in BALB‐c mice. The DH was 20% for the alcalase CAH and 50% for the pepsin‐pancreatin CAH, while the AoxA by ABTS (1 mg/mL) was 64.8% and 64.9% and by DPPH (5 mg/mL) was 48.0% and 31.1%. In the in vivo AoxA assay, mice of non‐damaged control and those treated with both CAH showed similar alkaline phosphatase values, control and pepsin‐pancreatin treated groups had similar malondialdehyde levels, while treated and non‐damaged control groups had higher glutathione levels than the damaged control. Liver histopathology revealed that the pepsin‐pancreatin CAH mitigated most of the pathological changes associated with the induced oxidative damage. Both CAH (2 mg/ear) reduced croton oil‐induced ear edema in mice. The α‐glucosidase inhibition of CAH (100 mg/mL) was 31.1% (alcalase) and 52.4% (pepsin‐pancreatin). Mice treated with alcalase CAH (100 mg/mL) and glibenclamide exhibited similar hypoglycemic activities, whereas only those treated with the pepsin‐pancreatin CAH (200 mg/kg body weight) showed anti‐hyperglycemic activity. The results indicate that CAH can be used as a source of bioactive peptides with antioxidant, anti‐inflammatory, hypoglycemic, and anti‐hyperglycemic activities. [ABSTRACT FROM AUTHOR]

Copyright of CyTA: Journal of Food is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Phenolic compounds are recognized for their antioxidant capacity and potential hypoglycemic activity. Thus, there is a need to develop more efficient extraction techniques. Phenolic extracts from Lippia graveolens aerial parts using supercritical CO2 (SC-CO2) extraction were obtained. We aimed to optimize the temperature and pressure conditions to obtain higher total phenolic content (TPC) from L. graveolens. Furthermore, we evaluated the total flavonoid content, antioxidant capacity, and α-amylase and α-glucosidase inhibition of the optimized supercritical fluid extract (OSFE). The optimal extraction conditions were 35.9 °C and 244.6 bar, with a TPC of 354 ± 8.6 mg GAE/g extract. The OSFE also showed higher TEAC and ORAC values than a conventional methanolic extract (ME); however the yield of the OSFE was lower than the yield of the ME, 5.9% and 25.9%, respectively. Despite that the ME showed 14 flavonoids and one phenolic acid compared to OSFE with 12 flavonoids and two phenolic acids, OSFE presented higher compounds content; both had α-glucosidase and α-amylase inhibitory activity. The use of SC-CO2 is an environment-friendly alternative to obtain extracts rich in phenolic compounds with beneficial bioactivities for human health. [ABSTRACT FROM AUTHOR]

The fruit of 18 Solanum lycopersicum var. cerasiforme accessions from Mexico were evaluated for total phenolics (TP) by the Folin-Ciocalteau assay, phenolic profiles by high performance liquid chromatography-diode array detection-mass spectrometry (HPLC-DAD-MS), antioxidant activity (AoxA) by 2,2´-azino-bis(3-ethylbenzothiazolin)-6-sulfonic acid (ABTS), 2,2-diphenil-1-pycrilhydrazyl (DPPH), and oxygen radical absorbance capacity (ORAC), and antimutagenic activity (AmuA) by the Ames assay. TP was measured as Gallic Acid Equivalents (GAE) and the AoxA as Trolox Equivalents (TE). TP varied from 37 to 86 mg GAE 100 g−1 fresh weight (fw). The AoxA by ABTS (568-1187 µmol TE 100 g−1 fw) and DPPH (157-350 µmol TE 100 g−1 fw) correlated with TP and the levels of caffeoylquinic acids and rutin. The AmuA did not correlate with the levels of phenolics. Some accessions had higher AoxA and AmuA than those reported for commercial cultivars and also showed high levels of caffeoylquinic acids and rutin; thus, their consumption could have good health promoting effects. [ABSTRACT FROM AUTHOR]

Abstract: The fruit of Vitex mollis Kunth contains water‐soluble melanin (SM), whose properties have been poorly described. This study determined physicochemical characteristics, the inhibition of carbohydrate digestive enzymes (amylase and α‐glucosidase), and antioxidant activity (ABTS, DPPH, ORAC, and β‐carotene bleaching) of SM (partially purified, aqueous extraction), water‐insoluble melanin (IM) (pure, alkali‐acid extraction), and organic (i.e., methanol, ethanol, and methanol : water 8:2 vol/vol) and aqueous extracts of V. mollis fruit. SM and IM were characterized by UV and IR spectroscopy, and their spectra showed signals characteristic of melanins. They also showed similar antioxidant (1,321–2,664 μmol TE/g), and α‐glucosidase inhibitory (IC50 1.48–0.022 mg/mL) activities, values that were better than those of the organic extracts (ABTS < 746 µmol TE/g and IC50 > 4.87 mg/mL, respectively). In addition, SMs showed higher extraction yields than IMs. Therefore, SM shares nutraceutical properties with IM and can be obtained by simple and environmentally friendly methods. Practical applications: Melanins show biological activities (e.g., antioxidant, immunostimulatory, UV‐, and radiation‐protective) and their consumption has been associated with health benefits. However, studies of plant melanins are scarce because their extraction involves harsh pH conditions and the yields are usually low. The results showed that melanin was the main pigment in Vitex mollis fruit and water‐soluble. Moreover, the extraction method was simple, ecological, and produced high yields. Both water‐soluble and water‐insoluble melanins showed similar biological activities (antioxidant and α glucosidase inhibition). Species of Vitex are commonly employed as food and in folk medicine (e.g., premenstrual and gynecological disorders, gastrointestinal problems and inflammation, bacterial infections). Soluble melanins of V. mollis could be used as food supplement or in pharmaceutical preparations. [ABSTRACT FROM AUTHOR]

Introduction. Pithecellobium dulce is a legume native to tropical America that produces edible arils which can be white or red. The plant is also grown in Asia and, to date, predominantly fruits produced in Asia have been the subject of scientific studies. We studied white and red arils produced in America. Materials and methods. White aril and red aril fruits were evaluated in an array of reagent-based assays to determine nutritional and nutraceutical properties. Results and discussion. White arils and red arils showed similar physicochemical characteristics, with high content of vitamin C (79.7–82.6 mg·100 g–1 fresh weight) and dietary fiber (5.83–6.12% fw). The anthocyanin content of red arils (29.5 mg·100 g–1 fw, as cyanidin-3-glucoside equivalents) was similar to that of strawberry. Total phenolics (517 mg·100 g–1 fw, as gallic acid equivalents) and antioxidant activities (ABTS, 224 mg; DPPH, 223 mg, as vitamin C equivalents) of red arils were 1.3 times higher than those in white arils. The methanolic extract of red arils showed a higher α-glucosidase inhibition (IC50 2.9 mg·mL–1) than acarbose (IC50 4.9 mg·mL–1). The methanolic extract [(50, 100 and 500) μg per tube] of red and white arils showed positive-strong antimutagenic activities (inhibition in the range 25–70%) in the assay (Salmonella typhimurium YG1024 strain, 1-nitropyrene as mutagen, 200 ng per tube). We are reporting for the first time remarkably high characteristics (i.e., antioxidant, inhibition of α-amylase and α-glucosidase and content of dietary fiber) of P. dulce fruits, mainly of the red ones; properties which combined permit us to suggest that consumption of these fruits could have beneficial health effects in people with diabetes. [ABSTRACT FROM AUTHOR]

Copyright of Revista Mexicana de Biodiversidad is the property of Universidad Nacional Autonoma de Mexico, Instituto de Biologia and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

We report for the first time the antioxidant and antimutagenic activities of fractions from Randia echinocarpa fruit, which is a Rubiaceae plant native to Sinaloa, Mexico. This fruit has been traditionally used in the prevention or treatment of cancer, among other diseases. The pulp of the fruit was sequentially extracted with solvents of different polarity (i.e. hexane, chloroform, methanol and water). A high extraction yield was obtained with methanol (72.17% d.w.). The aqueous extract showed the highest content of phenolics (2.27 mg/g as ferulic acid equivalents) and the highest antioxidant activity based on the β-carotene bleaching method (486.15). The commercial antioxidant BHT was used as control (835.05). Antimutagenic activity of the aqueous extract (0–500 μg/tube) was evaluated using the Salmonella microsuspension assay (YG1024 strain) and 1-NP as the mutagen (50 and 100 ng/tube). The aqueous extract was neither toxic nor mutagenic and the percentage of inhibition on 1-NP mutagenicity was 32 and 53% at doses of 50 and 100 ng/tube, respectively. The results of the double incubation assay suggest that the extract inhibited the mutagenicity of 1-NP by a combination of desmutagenic and bioantimutagenic effects. [ABSTRACT FROM AUTHOR]

The application of technologies to reduce papaya (Carica papaya L.) anthracnose, a disease caused by the fungus Colletotrichum gloeosporioides, most of the time results in a loss of nutrients and other bioactive compounds. This study was performed to determine the effect of hot water (HW; 48°C/20 min), calcium chloride (Ca; 1%/20 min), and their combination (HW‐Ca) in bioactive compounds and antioxidant activity of papaya inoculated with Colletotrichum gloeosporioides stored during 20 days at 12°C. HW‐Ca treated fruits showed higher ascorbic acid, phenolics content, and antioxidant activity than untreated and HW and Ca treated fruit, which correlated with the contents of ascorbic acid, phenolics, and β‐cryptoxanthin. Also, HW‐Ca treatment resulted in fruits showing lower decay area, greater firmness and color retention. The results suggest that HW‐Ca treatment could be used to reach higher antioxidant activity values and to preserve the postharvest quality parameters, whereas the development of anthracnose symptoms was delayed. Practical applications Papaya infected by Colletotrichum gloeosporioides and treated with hot water (HW) in combination with calcium chloride (Ca) had a good firmness, color retention, and higher antioxidant activity and content of ascorbic acid and phenolics, while the development of anthracnose symptoms was delayed. In this sense, the proposed HW‐Ca treatment of papaya is a strategy to protect against Colletotrichum gloeosporioides and to obtain fruits with good quality, resulting in an interesting postharvest alternative to the application of fungicides. [ABSTRACT FROM AUTHOR]

'Keitt' mango is one of the most important cultivars, and it is usually stored at a low temperature during its commercialization to extend shelf life and reach distant markets. However, it is susceptible to chilling injury (CI) and some prestorage treatments are required to reduce the incidence of this disorder. This research shows for the first time the protective effect of a combination hot water-calcium lactate (Ca) against CI in mango fruit cv. Keitt. Fruit were subjected to hot water treatment (HWT) (46.1 °C, 75-90 minutes) or treated with 0.5% Ca or with the combination HWT + Ca, stored at 5 °C for 20 days, and ripened at 21 °C for 7 days. CI index (CII), electrolyte leakage (EL), malondialdehyde (MDA) production, bioactive compounds, antioxidant capacity [2,2'-azino-bis-3-ethylbenzthiazoline-6-sulphonic acid (ABTS) and 2,2-diphenyl-1-picrylhydrazil (DPPH)], and activity of antioxidant enzymes [superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX)] were analyzed in mango samples after 0, 10, and 20 days of cold storage and after ripening. Hot water treatments (HWT and HWT + Ca) were more effective than Ca in providing protection against CI as evidenced by lower incidence of symptoms and lower EL and MDA. HWT + Ca increased the content of phenolics, flavonoids, and carotenoids during the cold storage, which correlated with the antioxidant capacity by ABTS. SOD and APX showed higher activity in HWT + Ca-treated fruit, whereas CAT activity was higher in fruit with HWT and Ca. These results suggest that HWT + Ca provided CI tolerance of 'Keitt' mango by activation of the enzymatic and nonenzymatic antioxidant systems. [ABSTRACT FROM AUTHOR]

• Hydroalcoholic extracts of Echeveria species (HE) show biological activities. • The best adaptogens and immunomodulators were the E. kimnachii and E. subrigida HE. • The best antioxidants were the E. subrigida and E. kimnachii HE. • The HE of E. craigiana, E. kimnachii, and E. subrigida were innocuous. • The best options for stress management were E. kimnachii and E. subrigida. Three Echeveria species (E. craigiana , EC; E. kimnachii , EK; and E. subrigida , ES) can potentially prevent/treat different diseases. This study aims to establish the following biological activities (evaluation assay) of their hydroalcoholic extracts (HE): adaptogenic (anoxia, forced-swim-endurance, and cold-swim-endurance in mice), immunomodulatory (splenocyte proliferation), antioxidant (ABTS, DPPH, and cellular-in- Saccharomyces cerevisiae), and toxicological (Artemia salina and acute in mice). EK and ES were the best adaptogens and immunomodulators (at 10 μg/mL: EK, 34%; ES, 20%). ES had the best antioxidant activities in vitro (DPPH, 1122.6 μmol TE/g, IC 50 = 200.7 μg/mL; ABTS, 2196.8 μmol TE/g, IC 50 = 165.8 μg/mL), and EK was the best in the Saccharomyces cerevisiae assay. The three HE of Echeveria were innocuous in the Artemia salina (LC 50 > 2000 mg/kg) and mice acute toxicity (LD 50 > 2 g/kg b.w.) assays. Thus, EK and ES showed the best potential for stress management. [ABSTRACT FROM AUTHOR]

In order to preserve plant biodiversity by its sustainable use, valuable plant products must be identified to induce the field production of specific species. Echeveria is the most representative genus from Crassulaceae of Mexico because its high endemism, historical–cultural significance and role in arid ecosystems of the country. Echeveria species have important biological activities, but their compounds have not been identified. The objective of the study was to determine biological activities ( i.e. , antimutagenic and antioxidant) and chemical composition by GC–MS of Echeveria kimnachii (EK) and Echeveria subrigida (ES) leaves. Methanol extracts of leaves were liquid–liquid partitioned to get the hexane (HF), ethyl acetate (EAF), and aqueous (AqF) fractions and they were analyzed. Methanol extracts and their fractions (500 μg/plate) showed mutagenicity inhibition (>45%) in the Ames assay using Salmonella Typhimurium YG1024 and 1-nitropyrene (200 ng/plate), and the most active fractions were HF-EK (91.8%) and EAF-ES (97.6%). The highest antioxidant activities (AA) were for EAFs by DPPH (inhibition %: EK = 67.5 and ES = 95.1) and β-carotene-bleaching (AA %: EK = 57.1 and ES = 41.6) methods. GC–MS profiles showed clear differences among EK and ES, hopenone b was specific for EK and brassicasterol and germanicol for ES. The high content of phenolics ( i.e. , isorhamnetin and gallic acid for ES) and organic acids ( i.e. , syringic and malic for EK) was associated with the highest antioxidant activities of the EAFs, and with the highest antimutagenic activity of EAF-ES. By contrast, HF-EK is high in sterols ( i.e. , lupenone, hopenone b and γ-sitosterol) which could be responsible of its strong antimutagenicity. Moreover, both Echeveria showed other secondary metabolites ( e.g. , phytosterols, terpenes and fatty acids) with published remarkable biological activities. Thus, E. kimnachii and E. subrigida could be sources of biological active compounds for pharmaceutical and nutraceutical industries. [ABSTRACT FROM AUTHOR]

Highlights: [•] Leaf extracts of Agave species from Sinaloa Mexico were evaluated for chemical composition and antioxidant- and antibacterial activities. [•] Agave ornithobroma had the higher content of phytochemicals. [•] The antibacterial activity of Agave tequilana and Agave schidigera was higher than those reported for other Agave species. [•] Agave rzedowskiana exhibited the highest antioxidant capacity by the DPPH method. [•] A bioguided purification strategy of a non-polar extract showed that α-tocopherol was the main responsible for the A. rzedowskiana antioxidant activity. [ABSTRACT FROM AUTHOR]