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2 results on '"Bezuglov, Vladimir"'

1. ACTH(6-9)PGP Peptide Protects SH-SY5Y Cells from H 2 O 2 , tert -Butyl Hydroperoxide, and Cyanide Cytotoxicity via Stimulation of Proliferation and Induction of Prosurvival-Related Genes.

Author

Akimov MG, Fomina-Ageeva EV, Dudina PV, Andreeva LA, Myasoyedov NF, and Bezuglov VV

Subjects
Cell Line, Tumor, Humans, Hydrogen Peroxide toxicity, Neuroprotective Agents pharmacology, Proline pharmacology, Reactive Oxygen Species metabolism, Adrenocorticotropic Hormone pharmacology, Antioxidants pharmacology, Apoptosis drug effects, Cell Proliferation drug effects, Oligopeptides pharmacology, Oxidative Stress drug effects, Proline analogs & derivatives
Abstract

Stabilized melanocortin analog peptide ACTH(6-9)PGP (HFRWPGP) possesses a wide range of neuroprotective activities. However, its mechanism of action remains poorly understood. In this paper, we present a study of the proproliferative and cytoprotective activity of the adrenocorticotropic hormone fragment 6-9 (HFRW) linked with the peptide prolyine-glycyl-proline on the SH-SY5Y cells in the model of oxidative stress-related toxicity. The peptide dose-dependently protected cells from H 2 O 2 , tert -butyl hydroperoxide, and KCN and demonstrated proproliferative activity. The mechanism of its action was the modulation of proliferation-related NF-κB genes and stimulation of prosurvival NRF2 -gene-related pathway, as well as a decrease in apoptosis.

Published
2021
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2. Antioxidant and neuroprotective properties of N-arachidonoyldopamine.

Author

Bobrov MY, Lizhin AA, Andrianova EL, Gretskaya NM, Frumkina LE, Khaspekov LG, and Bezuglov VV

Subjects
Animals, Animals, Newborn, Antioxidants chemistry, Antioxidants metabolism, Arachidonic Acids chemistry, Arachidonic Acids metabolism, Biological Assay, Brain metabolism, Cell Survival drug effects, Cell Survival physiology, Cells, Cultured, Culture Media, Serum-Free pharmacology, Cytoprotection physiology, Dopamine chemistry, Dopamine metabolism, Dopamine pharmacology, Dose-Response Relationship, Drug, Excitatory Amino Acid Antagonists pharmacology, Glutamic Acid toxicity, Molecular Structure, Neurons drug effects, Neurons metabolism, Neuroprotective Agents chemistry, Neuroprotective Agents metabolism, Peroxides metabolism, Rats, Rats, Wistar, Antioxidants pharmacology, Arachidonic Acids pharmacology, Brain drug effects, Cytoprotection drug effects, Dopamine analogs & derivatives, Neuroprotective Agents pharmacology
Abstract

N-Acyldopamines were recently described as putative endogenous substances in the rat brain. Among them, N-arachidonoyldopamine (AADA) was characterized as cannabinoid CB1 and vanilloid TRPV1 receptor ligand. The physiological significance of such compounds is yet poorly understood. In this study, we describe the novel properties of AADA as antioxidant and neuroprotectant. Antioxidant potential of AADA and its analogs were first tested in the galvinoxyl assay. It was found that N-acyldopamines are potent antioxidants and that the number of free hydroxyl groups in the phenolic moiety of dopamine is essential for the activity. AADA dose dependently (0.1-10 microM) protected cultured cerebellar granule neurons (CGN) in the model of oxidative stress induced by hydrogen peroxide. N-Oleoyldopamine, another endogenous substance, was much less potent in these conditions while the natural antioxidant alpha-tocopherol was inactive. In this test, AADA decreased the peroxide level in CGN preparations and its neuroprotection was independent of cannabinoid/vanilloid receptors blockade. AADA (10 microM) also protected CGN from death induced by K(+)/serum deprivation and glutamate exitotoxicity. These data indicate that AADA may act as endogenous antioxidant in different pathological conditions.

Published
2008
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