1. Addition of docosahexaenoic acid synergistically enhances the efficacy of apatinib for triple-negative breast cancer therapy.
- Author
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Ma Y, Yu J, Li Q, Su Q, and Cao B
- Subjects
- Antineoplastic Agents administration & dosage, Antineoplastic Agents pharmacology, Apoptosis drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Docosahexaenoic Acids administration & dosage, Docosahexaenoic Acids pharmacology, Drug Synergism, Female, Human Umbilical Vein Endothelial Cells, Humans, Proto-Oncogene Proteins c-akt metabolism, Pyridines administration & dosage, Pyridines pharmacology, Triple Negative Breast Neoplasms pathology, Antineoplastic Agents therapeutic use, Docosahexaenoic Acids therapeutic use, Pyridines therapeutic use, Triple Negative Breast Neoplasms drug therapy
- Abstract
The current study aimed to investigate the antitumor and antiangiogenesis effects of apatinib in triple-negative breast cancer in vitro and also whether the combination of docosahexaenoic acid (DHA) and apatinib is more effective than apatinib monotherapy. The cell counting kit-8 assay was used to measure cell proliferation. Flow cytometry was utilized to determine the cell apoptosis rate. A wound healing assay was utilized to assess cell migration. Western blot analysis was carried out to determine the effects of apatinib and DHA on Bcl-2, BAX, cleaved caspase-3, caspase-3, phosphorylated protein kinase B (p-Akt), and Akt expression. DHA in combination with apatinib showed enhanced inhibitory effects on cell proliferation and migration compared with apatinib or DHA monotherapy. Meanwhile, DHA combined with apatinib strongly increased the cell apoptosis percentage. DHA was observed to enhance the antitumor and antiangiogenesis effects of apatinib via further downregulation of p-Akt expression. Abbreviations : FITC: fluorescein isothiocyanate; PI: propidium iodide.
- Published
- 2020
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