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Addition of docosahexaenoic acid synergistically enhances the efficacy of apatinib for triple-negative breast cancer therapy.
- Source :
-
Bioscience, biotechnology, and biochemistry [Biosci Biotechnol Biochem] 2020 Apr; Vol. 84 (4), pp. 743-756. Date of Electronic Publication: 2019 Dec 31. - Publication Year :
- 2020
-
Abstract
- The current study aimed to investigate the antitumor and antiangiogenesis effects of apatinib in triple-negative breast cancer in vitro and also whether the combination of docosahexaenoic acid (DHA) and apatinib is more effective than apatinib monotherapy. The cell counting kit-8 assay was used to measure cell proliferation. Flow cytometry was utilized to determine the cell apoptosis rate. A wound healing assay was utilized to assess cell migration. Western blot analysis was carried out to determine the effects of apatinib and DHA on Bcl-2, BAX, cleaved caspase-3, caspase-3, phosphorylated protein kinase B (p-Akt), and Akt expression. DHA in combination with apatinib showed enhanced inhibitory effects on cell proliferation and migration compared with apatinib or DHA monotherapy. Meanwhile, DHA combined with apatinib strongly increased the cell apoptosis percentage. DHA was observed to enhance the antitumor and antiangiogenesis effects of apatinib via further downregulation of p-Akt expression. Abbreviations : FITC: fluorescein isothiocyanate; PI: propidium iodide.
- Subjects :
- Antineoplastic Agents administration & dosage
Antineoplastic Agents pharmacology
Apoptosis drug effects
Cell Line, Tumor
Cell Proliferation drug effects
Docosahexaenoic Acids administration & dosage
Docosahexaenoic Acids pharmacology
Drug Synergism
Female
Human Umbilical Vein Endothelial Cells
Humans
Proto-Oncogene Proteins c-akt metabolism
Pyridines administration & dosage
Pyridines pharmacology
Triple Negative Breast Neoplasms pathology
Antineoplastic Agents therapeutic use
Docosahexaenoic Acids therapeutic use
Pyridines therapeutic use
Triple Negative Breast Neoplasms drug therapy
Subjects
Details
- Language :
- English
- ISSN :
- 1347-6947
- Volume :
- 84
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Bioscience, biotechnology, and biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 31889475
- Full Text :
- https://doi.org/10.1080/09168451.2019.1709789