1. Synthesis and in vitro antitumor activity evaluation of copper(II) complexes with 5-pyridin-2-yl-[1,3]dioxolo[4,5-g]isoquinoline derivatives
- Author
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Liu-Yan Zhou, Qian-Qian Cao, Hong Liang, Zhen-Feng Chen, Wen-Ying Shen, Yun-Liang Zhang, Cai-Xing Deng, and Wen-Feng Zhou
- Subjects
Pyridines ,Stereochemistry ,Antineoplastic Agents ,Apoptosis ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Inorganic Chemistry ,HeLa ,chemistry.chemical_compound ,Coordination Complexes ,Organometallic Compounds ,Humans ,Enzyme Inhibitors ,Isoquinoline ,Cytotoxicity ,Telomerase ,chemistry.chemical_classification ,Reactive oxygen species ,biology ,010405 organic chemistry ,Cytochrome c ,Liver cell ,Hep G2 Cells ,biology.organism_classification ,In vitro ,Mitochondria ,0104 chemical sciences ,chemistry ,Quinolines ,biology.protein ,Copper ,HeLa Cells - Abstract
Seven Cu(II) complexes with 5-pyridin-2-yl-[1,3]dioxolo[4,5-g]isoquinoline derivatives as ligands: [Cu2(L1)2Cl4] (1), [Cu(L2)Cl2] (2), [Cu(L1)(NO3)2] (3), [Cu(L2)(NO3)2] (4), [Cu(L3)Cl2] (5), [Cu(L3)Br2] (6) and [Cu(L3)(NO3)2] (7){L1=9-nitro-5-pyridin-2-yl-[1,3]dioxolo[4,5-g]isoquinoline, L2=4-nitro-5-pyridin-2-yl-[1,3]dioxolo[4,5-g]isoquinoline, L3=9-bromo-5-pyridin-2-yl-[1,3]dioxolo[4,5-g]isoquinoline}, were synthesized and characterized. Their in vitro anticancer activities against T-24, MGC-80-3, HeLa, Hep-G2, A549 and SK-OV-3 were evaluated. Compared with their corresponding ligands, most of these complexes exhibited enhanced anticancer activities in contrast to their corresponding ligands and copper salt. Among them, complexes 1 and 3 displayed selective cytotoxicity to HeLa cells comparing with normal liver cell HL-7702, with IC50 values of 5.03 ± 1.20 μM and 10.05 ± 0.52 μM, respectively. Complexes 1 and 3 inhibited telomerase activity by interacting with c-myc promoter elements, and therefore exerted their antitumor activity. Furthermore, complexes 1 and 3 could trigger cell apoptosis via disruption of mitochondrial pathway through notably increased reactive oxygen species (ROS) levels, loss of mitochondrial membrane potential (Δψm), increase of the cytochrome c and apaf-1, decrease of bcl-2, and activation of caspases 3/9. Complexes 1 and 3 exhibited enhanced cytotoxicity, presenting synergetic effect after the ligands coordinated to copper(II) center.
- Published
- 2019