1. [Features of epidemiology and diagnostics of toxoplasmosis during HIV-infection].
- Author
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Gubareva EV, Goncharov DB, Peregudova AB, Parkhomenko IuG, Tishkevich IM, Tishkevich OA, and Kobets NV
- Subjects
- AIDS-Related Opportunistic Infections complications, Adult, Aged, Antibodies, Protozoan blood, Antibodies, Protozoan cerebrospinal fluid, Antigens, Protozoan analysis, Antigens, Protozoan immunology, Blotting, Western methods, Epitopes analysis, Epitopes immunology, Fluorescent Antibody Technique, Indirect, Humans, Immunoglobulin G analysis, Immunoglobulin G blood, Immunoglobulin G cerebrospinal fluid, Middle Aged, Russia epidemiology, Toxoplasma isolation & purification, Toxoplasmosis complications, Toxoplasmosis, Cerebral diagnosis, AIDS-Related Opportunistic Infections diagnosis, AIDS-Related Opportunistic Infections epidemiology, Antibodies, Protozoan analysis, HIV Infections complications, Toxoplasmosis diagnosis, Toxoplasmosis epidemiology
- Abstract
Aim: Comparative assessment of effectiveness of serologic methods for toxoplasmosis diagnostics in patients with HIV-infection., Materials and Methods: Sera and CSF samples from 166 patients with AIDS stage IIIB were tested for antibodies to Toxoplasma gondii by indirect immunofluorescence, ELISA and immunoblotting. Results of serological tests were compared with clinical, pathological data as well as with results of MRI and PCR., Results: Clinical value of IgG detection in blood and CSF by ELISA was shown--high level of antibodies marked reactivation of the invasion. IgG antibodies in CSF were detected only if high levels of IgG were present in the blood. Detection of antigenic determinants with molecular mass 18 - 20 and 65 - 70 kDa in immunoblotting was proposed as a criterion of cerebral toxoplasmosis reactivation., Conclusion: Complex laboratory serologic tests along with data obtained by MRI, PCR and microscopy of T. gondii cysts enhances the effectiveness of toxoplasmosis diagnostics. Knowledge of toxoplasmosis reactivation criteria in patients with AIDS will allow to develop the optimal protocol of toxoplasmosis diagnostics and substantiate measures for its prevention.
- Published
- 2010