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Phytochemical investigation of the stem bark of Stereospermum acuminatissimum resulted in the isolation of seventeen compounds, including four tirucallanes (1 – 4), three hopanes (5 – 7), one oleanane (8), three sterols (9 – 11), three saponins (12 – 14), two cinnamic acids (15 – 16), and one iridoid (17). The dehydration of compound 5 led to compound 5a. The structures of all compounds were elucidated by analysis of spectroscopic and mass spectrometric data. Compounds 1 , 6 and 7 are reported from the Bignoniaceae family for the first time, while compounds 11 – 14 and 16 are reported from S. acuminatissimum for the first time. The in vitro antibacterial activities of some compounds (1 – 5 , 8 , 5a), the crude extract and the ethyl acetate fraction were assessed. The crude extract showed significant antibacterial activity against Staphylococcus aureus , Salmonea pneumonia and Klebsiella pneumoniae with MIC values of 62 μg/mL. Compound 1 revealed moderate antibacterial activities against K. pneumoniae , Shigella flexneri and Methycillin resistant S. aureus with MIC values of 62, 62 and 31 μg/mL respectively. [Display omitted] • Phytochemical investigation of Stereospermum acuminatissimum K. Schum. • Dehydration of one of the isolated compound. • Chemophenetic significance of the isolated compounds. • Antibacterial activities of crude extract, fractions and compounds. [ABSTRACT FROM AUTHOR]

The phytochemical study was done on the leaves of Gambeya lacourtiana. This plant has been used in traditional medicine to treat of different ailments such as uterine heamorrhage, metritis and other vaginal infections. Chromatographic fractionation and purification on the leaves crude extract afforded lupeol acetate (1), lupenone (2), lupeol (3), taraxerol (4) stigmasterol (5), erythrodiol (6), chamaedrydiol (7), methyl pheophorbide-a (8), corosolic acid (9), tormentic acid (10), epicatechin (11) and 22-dihydrospinasterol 3- O - β - D -glucopyranoside (12). The structures of compounds 1 – 12 were elucidated on the basis of 1D and 2D NMR, mass spectrometric and the spectroscopic data as well as comparison with the literature. Compounds 4 , 7 – 10 and 12 were isolated for the first time from Gambeya genus. Crude extract, fractions and compounds 6–12 were evaluated for their antibacterial activity. Methyl pheophorbide-a (8) demonstrated moderate activity against Salmonella typhi CPC with MIC values of 62.5 μg/mL. The chemophenetic significance of these compounds is also discussed. [Display omitted] • Isolation of 12 compounds from the leaves of Gambeya lacourtiana (Sapotaceae). • First report of a chlorophyll type skeleton in the genus Gambeya. • First antibacterial report of the leaves of Gambeya lacourtiana. [ABSTRACT FROM AUTHOR]