1. A Meloidogyne incognita C‐type lectin effector targets plant catalases to promote parasitism
- Author
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Qinghua Sun, Jian Ling, Michaël Quentin, Yan Li, Zhang Xiaoping, Bingyan Xie, Pierre Abad, Jianlong Zhao, Bruno Favery, Yuhong Yang, Zhenchuan Mao, Institute of Vegetables and Flowers (IVF), Chinese Academy of Agricultural Sciences (CAAS), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Chifeng University, 45478PF/French-Chinese bilateral collaboration program PHC XU GUANGQI 2020IVF-BRF2021011/Central Public-interest Scientific Institution Basal Research Fund for Chinese Academy of Agricultural SciencesCAAS-ASTIP-2017-IVF/Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences, ANR-11-LABX-0028,SIGNALIFE,Réseau d'Innovation sur les Voies de Signalisation en Sciences de la Vie(2011), and ANR-15-IDEX-0001,UCA JEDI,Idex UCA JEDI(2015)
- Subjects
0106 biological sciences ,Physiology ,Arabidopsis ,interaction ,Nicotiana benthamiana ,Plant Science ,reactive oxygen species (ROS) ,01 natural sciences ,03 medical and health sciences ,C-type lectin ,Meloidogyne incognita ,Animals ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Lectins, C-Type ,Tylenchoidea ,Plant Diseases ,030304 developmental biology ,0303 health sciences ,biology ,Effector ,catalase ,fungi ,food and beverages ,Helminth Proteins ,biology.organism_classification ,Cell biology ,effector ,Catalase ,biology.protein ,MiCTL1 ,Ectopic expression ,Terra incognita ,010606 plant biology & botany - Abstract
International audience; Root-knot nematodes, Meloidogyne spp., secrete effectors to modulate plant immune responses and establish a parasitic relationship with host plants. However, the functions and plant targets of C-type lectin (CTL)-like effectors of Meloidogyne incognita remain unknown. Here, we characterized a CTL-like effector of M. incognita, MiCTL1a, and identified its target and role in nematode parasitism. In situ hybridization demonstrated the expression of MiCTL1 in the subventral glands; and in planta, immunolocalization showed its secretion during M. incognita parasitism. Virus-induced gene silencing of the MiCTL1 reduced the infection ability of M. incognita in Nicotiana benthamiana. The ectopic expression in Arabidopsis not only increased susceptibility to M. incognita but also promoted root growth. Yeast two-hybrid and co-immunoprecipitation assays revealed that MiCTL1a interacts with Arabidopsis catalases, which play essential roles in hydrogen peroxide homeostasis. Knockout or overexpression of catalases showed either increased or reduced susceptibility to M. incognita, respectively. Moreover, MiCTL1a not only reduced catalase activity in vitro and in planta but also modulated stress-related gene expressions in Arabidopsis. Our data suggest that MiCTL1a interacts with plant catalases and interferes with catalase activity, allowing M. incognita to establish a parasitic relationship with its host by fine-tuning responses mediated by reactive oxygen species.
- Published
- 2021