1. Sperm viability, serological, molecular, and modified seminal plasma agglutination tests in the diagnosis of Leptospira in the semen and serum of bovine bulls
- Author
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Geraldo de Nardi Junior, Antonio Francisco de Souza Filho, Rogério Giuffrida, Gustavo Henrique Batista Lara, Marcelo George Mungai Chacur, Lorrayne de Souza Araújo Martins, Adriana Cortez, Fabio Morato Monteiro, Joeleni Rosa dos Santos, Sérgio Ricardo Maiolino, Rodrigo Garcia Motta, Márcio Garcia Ribeiro, Helio Langoni, Thaís Spessotto Bello, Marcos Bryan Heinemann, Universidade Estadual Paulista (UNESP), Santo Amaro University - UNISA, University of Oeste Paulista, Technology Faculty - FATEC, Institute of Animal Science, and Universidade de São Paulo (USP)
- Subjects
Male ,Serum ,endocrine system ,animal diseases ,Seroprevalence ,Semen ,Biology ,Microbiology ,Serology ,Andrology ,Blood serum ,Agglutination Tests ,RNA, Ribosomal, 16S ,Direct agglutination test ,Media Technology ,medicine ,Animals ,Leptospirosis ,Reproduction by natural means ,Leptospira ,Azoospermia ,SÊMEN ANIMAL ,urogenital system ,Veterinary Microbiology - Research Paper ,medicine.disease ,Spermatozoa ,Sperm ,Agglutination (biology) ,Bull leptospirosis ,Necrospermia ,Cattle ,Molecular diagnosis - Abstract
Made available in DSpace on 2022-05-01T08:44:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The present study investigated the serum microscopic agglutination test (MAT) among 203 bovine bulls with reproduction by natural means, without apparent signs of orchitis or inflammation of accessory reproductive glands. Simultaneously, the semen of all bulls was subjected to sperm viability analysis and PCR based on the 16S rRNA gene. PCR-positive results of semen samples were confirmed by sequencing. A modified seminal plasma agglutination (MSPA) test, replacing the blood serum of all bulls in the MAT with seminal plasma was performed as well. Eight (8/203 = 3.9%) semen samples from bulls were considered nonviable (necrospermia and azoospermia) without relation to the PCR diagnosis. No agglutinin titers were identified in MSPA test. A high frequency (132/203 = 65%) of leptospiral agglutinin titers was identified in the MAT, particularly for the Sejroe serogroup (Hardjo CTG, 100/203 = 49.3%; Wolffi 74/203 = 36.4%; Guaricura 72/203 = 35.5%; and Hardjoprajitno 56/203 = 27.6%). Three (3/203 = 1.5%) semen samples of bulls were positive in the PCR, but these results were not confirmed by sequencing. The high frequency of serovars from the Sejroe serogroup typically adapted to bovines indicates the need for measures for the prophylaxis/control of the pathogen on the sampled farms. Discrepancies among the MAT, sperm viability, and molecular detection of leptospires in semen highlight the need for a combination of methods to diagnose leptospirosis in bovine bulls. To our knowledge, modified seminal plasma agglutination is described for the first time here to investigate anti-Leptospira antibodies produced locally in the genital tract in the diagnosis of bovine leptospirosis among bulls that reproduce by natural means. Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP Santo Amaro University - UNISA Faculty of Veterinary Medicine University of Oeste Paulista Technology Faculty - FATEC APTA Beef Cattle Center Institute of Animal Science University of São Paulo - USP Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP
- Published
- 2021
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