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Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk
Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk
- Source :
- International journal of systematic and evolutionary microbiology (Online) 67 (2017): 3865–3871. doi:10.1099/ijsem.0.002209, info:cnr-pdr/source/autori:Morandi Stefano; Cremonesi Paola; Povolo Milena; Capra Emanuele; Silvetti Tiziana; Castiglioni Bianca; Ribeiro Marcio Garcia; Alves Ana Carolina; da Costa Geraldo Marcio; Luini Mario; Brasca Milena/titolo:Prototheca blaschkeae subsp. brasiliensis subsp. nov., isolated from cow milk./doi:10.1099%2Fijsem.0.002209/rivista:International journal of systematic and evolutionary microbiology (Online)/anno:2017/pagina_da:3865/pagina_a:3871/intervallo_pagine:3865–3871/volume:67, Scopus, Repositório Institucional da UNESP, Universidade Estadual Paulista (UNESP), instacron:UNESP
- Publication Year :
- 2017
- Publisher :
- Microbiology Society, 2017.
-
Abstract
- Made available in DSpace on 2018-12-11T17:34:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-10-01 A strain of an achlorophyllic alga, named PR24T, was isolated from cow milk samples from the state of Minas Gerais, Brazil. Based on 18S rDNA, 28S rRNA, D1/D2 region of the LSU rDNA and SSU rRNA gene sequence similarities, this strain was found to be a member of the genus Prototheca and closely related to Prototheca blaschkeae SAG2064T. However, the novel strain could easily be distinguished from recognized Prototheca species by internal transcribed spacer, species-specific PCR, single-strand conformation polymorphism-PCR analysis and phenotypic characteristics. The inability to grow in Sabouraud broth at pH 4.0 and the different cellular fatty acid composition clearly distinguished PR24T from the reference strain of P. blaschkeae. The combination of genotypic and phenotypic data indicates that strain PR24T represents a subspecies of P. blaschkeae, for which the name Prototheca blaschkeae subsp. brasiliensis subsp. nov. is proposed. The respective type strain is PR24T (=DSM 103592T=IHEM 26958T). Institute of Sciences of Food Production Italian National Research Council (CNR ISPA), Via Celoria 2 Institute of Agricultural Biology and Biotechnology Italian National Research Council (CNR IBBA), Via Einstein Council for Agricultural Research and Economics – Research Centre for Animal Production and Aquaculture (CREA-ZA), Via Lombardo 11 Department of Veterinary Hygiene and Public Health Universidade Estadual Paulista (UNESP) Department of Veterinary Medicine Federal University of Lavras Istituto Zooprofilattico Sperimentale della Lombardia e dell’Emilia Romagna (IZSLER), Via Einstein Department of Veterinary Hygiene and Public Health Universidade Estadual Paulista (UNESP)
- Subjects :
- 0301 basic medicine
Cow milk
030106 microbiology
Prototheca
Biology
Subspecies
Microbiology
Prototheca blaschkeae
03 medical and health sciences
DNA, Algal
Prototheca blaschkeae subsp. brasiliensis
28S ribosomal RNA
RNA, Ribosomal, 28S
Genotype
Botany
RNA, Ribosomal, 18S
Animals
Internal transcribed spacer
Mastitis, Bovine
Phylogeny
Ecology, Evolution, Behavior and Systematics
Ssu rrna gene
Protothecal bovine mastitis
Base Composition
Strain (chemistry)
Fatty Acids
Sequence Analysis, DNA
General Medicine
protothecal bovine mastitis
cow milk
Milk
030104 developmental biology
Cattle
Female
Brazil
Subjects
Details
- ISSN :
- 14665034 and 14665026
- Volume :
- 67
- Database :
- OpenAIRE
- Journal :
- International Journal of Systematic and Evolutionary Microbiology
- Accession number :
- edsair.doi.dedup.....f242a0c76b6a846c5078ea4e7e74f8f9
- Full Text :
- https://doi.org/10.1099/ijsem.0.002209