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Your search keyword '"Kinase activity"' showing total 2 results
2 results on '"Kinase activity"'

1. Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei.

Author

Liu, Peng-fei, Liu, Qing-hui, Wu, Yin, and Huang, Jie

Subjects
*NUCLEOSIDE diphosphate kinases, *WHITE spot syndrome virus, *WHITELEG shrimp, *DNA replication, *DNA copy number variations, *DISEASES
Abstract

Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP’s) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection. After synthesizing dsRNA-NDK and confirming the efficacy of NDK silencing, we recorded the cumulative mortality of WSSV-infected shrimp injected with NDK and dsRNA-NDK. A comparison between the results demonstrated that silencing NDK delayed the death of shrimps. These findings indicate that NDK has an important role influencing the replication of WSSV replication in shrimp. Furthermore, NDK may have potential target as a new therapeutic strategy against WSSV infection in shrimp. [ABSTRACT FROM AUTHOR]

Published
2017
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2. Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei

Author

Peng-fei Liu, Qing-Hui Liu, Jie Huang, and Yin Wu

Subjects
0301 basic medicine, Hemocytes, White spot syndrome, Gene Dosage, Glycobiology, Gene Expression, lcsh:Medicine, Artificial Gene Amplification and Extension, Polymerase Chain Reaction, Biochemistry, law.invention, White Blood Cells, RNA interference, Animal Cells, law, Medicine and Health Sciences, lcsh:Science, Multidisciplinary, biology, Nucleosides, 04 agricultural and veterinary sciences, Complementary DNA, Recombinant Proteins, Glycosylamines, Nucleoside-diphosphate kinase, Crustaceans, Shrimp, Nucleic acids, Genetic interference, Organ Specificity, Recombinant DNA, Epigenetics, Cellular Types, Research Article, animal structures, Arthropoda, Forms of DNA, Immune Cells, Immunology, Research and Analysis Methods, Virus, 03 medical and health sciences, White spot syndrome virus 1, Extraction techniques, Penaeidae, Genetics, Animals, Molecular Biology Techniques, Molecular Biology, Blood Cells, lcsh:R, fungi, Organisms, DNA replication, Biology and Life Sciences, Cell Biology, DNA, biology.organism_classification, Invertebrates, Virology, RNA extraction, Enzyme Activation, 030104 developmental biology, Nucleoside-Diphosphate Kinase, 040102 fisheries, RNA, 0401 agriculture, forestry, and fisheries, lcsh:Q, Nucleoside
Abstract

Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP’s) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection. After synthesizing dsRNA-NDK and confirming the efficacy of NDK silencing, we recorded the cumulative mortality of WSSV-infected shrimp injected with NDK and dsRNA-NDK. A comparison between the results demonstrated that silencing NDK delayed the death of shrimps. These findings indicate that NDK has an important role influencing the replication of WSSV replication in shrimp. Furthermore, NDK may have potential target as a new therapeutic strategy against WSSV infection in shrimp.

Published
2017

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