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Increased nucleoside diphosphate kinase activity induces white spot syndrome virus infection in Litopenaeus vannamei
- Source :
- PLoS ONE, Vol 12, Iss 5, p e0175741 (2017), PLoS ONE
- Publication Year :
- 2017
- Publisher :
- Public Library of Science (PLoS), 2017.
-
Abstract
- Nucleoside diphosphate kinase (NDK), which has the same sequence as oncoprotein (OP) in humans, can induce nucleoside triphosphates in DNA replication by maintenance of the deoxynucleotide triphosphate (dNTP’s) and is known to be regulated by viral infection in the shrimp Litopenaeus vannamei. This paper describes the relationship between NDK and white spot syndrome virus (WSSV) infection. The recombinant NDK was produced by a prokaryotic expression system. WSSV copy numbers and mRNA levels of IE1 and VP28 were significantly increased in shrimp injected with recombinant NDK at 72 h after WSSV infection. After synthesizing dsRNA-NDK and confirming the efficacy of NDK silencing, we recorded the cumulative mortality of WSSV-infected shrimp injected with NDK and dsRNA-NDK. A comparison between the results demonstrated that silencing NDK delayed the death of shrimps. These findings indicate that NDK has an important role influencing the replication of WSSV replication in shrimp. Furthermore, NDK may have potential target as a new therapeutic strategy against WSSV infection in shrimp.
- Subjects :
- 0301 basic medicine
Hemocytes
White spot syndrome
Gene Dosage
Glycobiology
Gene Expression
lcsh:Medicine
Artificial Gene Amplification and Extension
Polymerase Chain Reaction
Biochemistry
law.invention
White Blood Cells
RNA interference
Animal Cells
law
Medicine and Health Sciences
lcsh:Science
Multidisciplinary
biology
Nucleosides
04 agricultural and veterinary sciences
Complementary DNA
Recombinant Proteins
Glycosylamines
Nucleoside-diphosphate kinase
Crustaceans
Shrimp
Nucleic acids
Genetic interference
Organ Specificity
Recombinant DNA
Epigenetics
Cellular Types
Research Article
animal structures
Arthropoda
Forms of DNA
Immune Cells
Immunology
Research and Analysis Methods
Virus
03 medical and health sciences
White spot syndrome virus 1
Extraction techniques
Penaeidae
Genetics
Animals
Molecular Biology Techniques
Molecular Biology
Blood Cells
lcsh:R
fungi
Organisms
DNA replication
Biology and Life Sciences
Cell Biology
DNA
biology.organism_classification
Invertebrates
Virology
RNA extraction
Enzyme Activation
030104 developmental biology
Nucleoside-Diphosphate Kinase
040102 fisheries
RNA
0401 agriculture, forestry, and fisheries
lcsh:Q
Nucleoside
Subjects
Details
- ISSN :
- 19326203
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- PLOS ONE
- Accession number :
- edsair.doi.dedup.....1c6f0c5692bf9892e2f3882436759569