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Maize is an important feed and industrial cereal crop and is crucial for global food security. The development of drought-tolerant genotypes is a major aim of breeding programs to fight water scarcity and maintain sustainable maize production. Late embryogenesis abundant (LEA) proteins are a family of proteins related to osmotic regulation that widely exist in organisms. Here, we implemented a previously generated maize transcriptomic dataset to identify a drought-responsive gene designated ZmNHL1. Bioinformatics analysis of ZmNHL1 showed that the protein encoded by ZmNHL1 belongs to the LEA-2 protein family. Tissue specific expression analysis showed that ZmNHL1 is relatively abundant in stems and leaves, highly expressed in tassels and only slightly expressed in roots, pollens and ears. Moreover, the activity of SOD and POD of plants from three 35S::ZmNHL1 transgenic lines under either the induced drought stress conditions (by 20% PEG6000) or the natural water deficit treatment (by water withholding) were higher than that of the WT plants, while the electrolyte leakage of the 35S::ZmNHL1 transgenic plants was lower than that of the WT plants under both drought treatments. Our data further revealed that ZmNHL1 promotes maize tolerance to drought stress in 35S::ZmNHL1 transgenic plants by improving ROS scavenging and maintaining the cell membrane permeability. Overall, our data revealed that ZmNHL1 promotes maize tolerance to drought stress and contributes to provide elite germplasm resources for maize drought tolerance breeding programs.

Serine/threonine protein phosphatases play essential roles in plants. PP2C has diverse functions related to development and stress response, while little is known about the functions of PP2C genes with respect to a variety of stresses in maize. In the present study, three ZmPP2C genes, ZmPP2C55, ZmPP2C28, and ZmPP2C71, were identified. Subcellular localization demonstrated that ZmPP2C28 and ZmPP2C71 were nuclear proteins, and ZmPP2C55 was located in both the nucleus and cytoplasm. qRT–PCR analysis showed that ZmPP2C55, ZmPP2C28, and ZmPP2C71 were expressed in roots, leaves and stems, and the three genes were responsive to drought, salt, high-temperature stress and exogenous ABA treatment. To explore the function of the ZmPP2C gene, ZmPP2C55-overexpressing transgenic lines were generated. The transgenic plants exhibited higher RWC, proline content, POD and SOD activities, GSH content and GSH/GSSG ratio and lower MDA content, electrolyte leakage and GSSG content compared with WT plants under natural stress treatment when seedlings were at the three-leaf. Our results illustrated that the overexpression of ZmPP2C55 positively enhanced tolerance to drought stress.

A field experiment was conducted to explore the effect of different sowing time and seeding rate on yield components and water use efficiency (WUE) of winter wheat by regulating the growth redundancy and physiological traits of root and shoot. Winter wheat sowing was performed on Oct.12 (T1), Oct.22 (T2), Nov.3 (T3), respectively. There were three treatments of different seeding rates, i.e. 112.5 kg hm−2 (D1), 150 kg hm−2 (D2) and 225 kg hm−2(D3) in each sowing time. The results showed that sowing time and seeding rate significantly influenced the morphological and physiological characteristics of winter wheat. Plant height, ineffective tiller number, leaf area and root biomass decreased significantly along with the delay of sowing date. Plant height, ineffective tiller number and root biomass increased significantly and leaf area decreased significantly with increasing seeding rate. Under the same seeding rate condition, T1 had the highest number of ineffective tiller, and T3 had the lowest one. Delayed sowing (T2 and T3) increased significantly root activities and leaf photosynthetic rate (Pn) in the late growing stage, and decreased significantly root respiration rate (Rroot) per unit area of wheat. In the same sowing time treatment, root activities and leaf Pn decreased significantly and Rroot increased significantly with the increase of seeding rate; however, seeding rate had no effect on root activity and leaf Pn in T3 treatment. Grain yield decreased significantly in T1 and increased significantly in T3 with the increase of seeding rate, and it was the highest in D2 and the lowest in D1 in T2 treatment. Delayed sowing lowered significantly soil water consumption of crop. Under the same sowing time condition, soil water consumption increased significantly as seeding rate increased. WUE decreased significantly with the increase of seeding rate in T1and T2 treatments. In two test years, T2D2 had highest grain yield and WUE, and T3D1 had lowest grain yield and WUE. The interaction of sowing time and seeding rate also had significant effect on soil water consumption, grain yield and WUE. It is clear that appropriate sowing time and seeding rate (T2 Treatmeat) can increase grain yield and WUE of winter wheat by regulating the growth redundancy and physiological traits of root and shoot, but excessive reduction of vegetative organs (T3 Treatmeat) can affect crop production.

Increasing planting density is an important ways to increase maize yield. A hot topic of conversation in the current research is how to improve crop light efficiency and yield potential by optimizing the cultivation mode under high density planting is a hot topic in current research. Thus, in this study, a field experiment was conducted to explore the effects of stereo-planting patterns on water and the utilization light resource and maize yields. Planting patterns included the conventional flat planting pattern (as the control, CK) and the stereo-planting in ridge and furrow (T). Each planting pattern had three planting densities, i.e., 60,000 plants ha−1 (D1), 75,000 plants ha−1 (D2) and 90,000 plants ha−1 (D3). The results showed that stereo-planting affected the physiological characteristics of plants by changing the spatial distribution of soil moisture. At the silking stage (R1), photosynthetic rate (Pn) of plants on the ridge was similar to CK, and transpiration rate (Tr) was significantly lower than that of CK. Pn of maize in the furrow was significantly higher than that of CK, and Tr was similar to CK. Stereoscopic planting had different effects on intraspecific competition intensity in maize population in different growing stages. In the six-leaf stage (V6), stereo-planting increased competition intensity of maize on the ridge, but lowered that of maize in the furrow by affecting the spatial distribution of soil moisture. During the R1 stage, stereo-planting increased the light transmittance rate within the canopy and eased the plant’s competition for light by reducing plant height and leaf area of maize under three density conditions. Stereo-planting had no effect on grain yield and dry matter accumulation of ridge-planted maize in the later growing stage, but it did increased the dry matter accumulation and grain yield of furrow-planted maize due to the improvement of the light environment and photosynthetic characteristics of the population. In two test years, stereo-planting increased 5.0–11.0% average yield of maize compared to CK under three density conditions. These results indicate that stereo-planting can reduce the plant’s competition for light and water resources and improve its physiological traits of plant by optimizing its spatial distribution of soil moisture and canopy structure, thus further increasing grain yield of maize under high-density planting conditions.

Excessive water and fertilizer input in conventional farming practices not only lead to low production but also damaged the ecosystem by causing groundwater decline and groundwater nitrate contamination. The two-year field experiments were conducted at Zhengzhou of China and TandoJam of Pakistan sites, respectively. The WHCNS (soil-water-heat-carbon-nitrogen-simulator) model was used to simulate soil water dynamic, nitrogen (N) fate and maize yield under different irrigation practices, including farmer-based full flood irrigation (FI), drip irrigation (DI) and rainfed (RF) at the Zhengzhou site. Treatments of the TandoJam site were full flood irrigation (F100), moderate deficit irrigation (F75) and high deficit irrigation (F50) to meet the 100%, 75% and 50% crop evapotranspiration, respectively. Results showed that the model precisely simulated water consumption, fates of N, crop growth, and yield at both sites. In Zhengzhou site, we found the lowest grain yield in the RF system, whereas the DI increased average grain yield by 9.8%, decreased 62.5% water consumption compared to FI for the two years. Moreover, the DI had a negligible amount of drainage, runoff, and N leaching, which subsequently improved water use efficiency (WUE) up-to 24.5% and 9.1% fertilizer N use efficiency (FNUE). In TandoJam site, compared to F100, the F75 produced almost equal grain yield with 25.1% water-saving, reduced 42.5% drainage and 11.8% runoff, 57% N leaching, enhanced 4.1% WUE and 14.9% FNUE. Furthermore, results suggested that too deficit irrigation, for example, F50, could cause serious yield loss or complete crop failure, because the weather condition of the TandoJam site is very hot.

In the rain-fed areas of northern China, maize ( Zea mays L.) is a main field crop, as it is well adapted to high temperatures and bright sunshine. However, low and variable rainfall and high evapotranspiration rates are common in water-limited environments during the growing season, and often mismatched rainfall events with the critical growth stages, making yield unstable. In this study, the performance of a furrow-planting and straw-mulching system was compared with the conventional flat-planting system in a double-crop culture of winter wheat ( Triticum aestivum L.) and summer maize for two consecutive years (2005–2006 and 2006–2007). The four tested treatments were: conventional flat planting (F), furrow planting between ridges (B), flat planting with wheat straw-mulching (FS), and furrow planting between ridges with wheat-straw mulch (BS). Soil water content and leaf area index (LAI) were measured throughout the growing season each year, and grain yield and precipitation-use efficiency (PUE Y ) were determined. On average, ridge tillage combined with furrow planting increased maize yield by 430 kg ha −1 (7.3%) and PUE Y by 10.7% (1.5 kg ha −1 mm −1 ), compared with the conventional flat planting; furrow planting coupled with straw mulching increased yield by an additional 16.9% and PUE Y by 19.4%, respectively. From jointing to maturity, LAI values of BS were significantly higher than those of F-system (55.6% vs. 26.1% in 2006 and 81.4% vs. 21.7% in 2007). Our data suggest that maize production adopted by furrow planting with straw-covered ridges performed best under seasonal average rainfall below 480 mm, which was associated with better synchronization of seasonal soil water supply and crop needs, leading to improved maize yield and PUE Y .