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Overexpression of ZmPP2C55 positively enhances tolerance to drought stress in transgenic maize plants
- Source :
- Plant science : an international journal of experimental plant biology. 314
- Publication Year :
- 2021
-
Abstract
- Serine/threonine protein phosphatases play essential roles in plants. PP2C has diverse functions related to development and stress response, while little is known about the functions of PP2C genes with respect to a variety of stresses in maize. In the present study, three ZmPP2C genes, ZmPP2C55, ZmPP2C28, and ZmPP2C71, were identified. Subcellular localization demonstrated that ZmPP2C28 and ZmPP2C71 were nuclear proteins, and ZmPP2C55 was located in both the nucleus and cytoplasm. qRT–PCR analysis showed that ZmPP2C55, ZmPP2C28, and ZmPP2C71 were expressed in roots, leaves and stems, and the three genes were responsive to drought, salt, high-temperature stress and exogenous ABA treatment. To explore the function of the ZmPP2C gene, ZmPP2C55-overexpressing transgenic lines were generated. The transgenic plants exhibited higher RWC, proline content, POD and SOD activities, GSH content and GSH/GSSG ratio and lower MDA content, electrolyte leakage and GSSG content compared with WT plants under natural stress treatment when seedlings were at the three-leaf. Our results illustrated that the overexpression of ZmPP2C55 positively enhanced tolerance to drought stress.
- Subjects :
- Crops, Agricultural
Genotype
Plant Science
Genetically modified crops
Biology
Genes, Plant
Zea mays
chemistry.chemical_compound
Gene Expression Regulation, Plant
Genetics
Proline
Nuclear protein
Gene
Genetically modified maize
Dehydration
food and beverages
Genetic Variation
Natural stress
General Medicine
Glutathione
Subcellular localization
Plants, Genetically Modified
Adaptation, Physiological
Cell biology
Droughts
chemistry
Agronomy and Crop Science
Subjects
Details
- ISSN :
- 18732259
- Volume :
- 314
- Database :
- OpenAIRE
- Journal :
- Plant science : an international journal of experimental plant biology
- Accession number :
- edsair.doi.dedup.....9f98e672564785d2e6285be88f0c3326