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Co-composting is the controlled aerobic degradation of organics, using more than one feedstock. By combining the spent mushroom substrate of Pholiota nameko (SMS) and pig manure (PM), the benefits of each could be used to optimize the composting process and the final product. This study introduced a comprehensive evaluation strategy aimed at identifying the optimal co-composting ratio for these two substrates. A 120-day composting trial was conducted, blending SMS and PM in various ratios to evaluate the benefits of co-composting SMS-PM. The results indicated that dissolved organic matter (DOM) in SMS-derived compost primarily originated from plants, whereas PM-derived compost predominantly consisted of microbial metabolic products, and co-composting combined both sources. An increase in aromaticity and humification degree of DOM occurred during the composting process itself rather than being derived from autochthonous origin. Carbohydrates like phenols and alcohols broke down during composting, and microbes utilized polysaccharides as an energy source for humus formation. As co-composting progressed, the treatments with varying mass ratios of SMS to PM, including 8:2, 7:3, 6:4, 5:5, 4:6, and 3:7 were observed to result in a decline in aliphatic hydroxylated chains alongside an enhancement in aromaticity within the compost. Additionally, there was a conversion from organic carbon (C) to carboxyl C within humic acid (HA) due to oxidation and dehydrogenation processes that facilitated the formation of stable nitrogen-containing compounds characterized by condensed aromatic structures. Following thorough evaluation, it was determined that optimal composting efficacy occurred at a mass ratio of SMS to PM equal to 6:4. Post-compost analysis revealed increases in nutrient content; specifically, germination index (GI) value reached 132.7%, while organic matter content attained 45.3%. Conversely, electrical conductivity (EC), C contents of water-soluble substances and humin (Cwss and C Hu ) decreased by approximately 11.8%, 73.4%, and 29.8% respectively; meanwhile, C contents of humic-extracted acid and HA (C HE and C HA ), along with degree of polymerization (DP), increased by 17.3%, 20.3% and 9.9% respectively. The proposed co-compost formula not only facilitated simultaneous recycling of both SMS and PM waste but also transformed them into high-quality organic fertilizers suitable for soil enrichment-effectively addressing challenges faced by both edible fungi cultivation and livestock industries while augmenting organic fertilizer sources for Black land protection., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 Elsevier Ltd. All rights reserved.)

Spent mushroom substrates (SMS) have been increasingly applied as organic fertilizer worldwide. However, the effects of various SMS on the accumulation and speciation characteristics of soil heavy metal(loid)s (HMs) are generally overlooked. Three types of SMS, including Flammulina velutipes residue (FVR), Agaricus bisporus residue (ABR), and Auricularia auricula residue (AAR), were applied to replace 25 % and 50 % of chemical fertilizer (based on nitrogen application) used in maize-wheat farmland. Compared to chemical fertilizer, the soil Cd, Pb, and As concentrations were decreased by 20.41 %, 5.97 %, and 10.09 %, respectively. And the residual fractions of soil HMs were increased through the application of SMS, indicating a reduction in their bioavailability. Notably, 50 % ABR replacement significantly increased the proportion of residual fraction in soil Cd, Pb, and As by 23.03 %, 15.15 %, and 4.85 %, respectively (P<0.05). A significant negative correlation was observed between the concentrations of HMs in grains and the residual fractions of soil HMs. Thus, compared with chemical fertilizers, the residual fractions of soil HMs were increased by the application of SMS, thereby reducing the concentrations of HMs in grains. Ingestion of crops is the primary route for human exposure to HMs. Therefore, the application of SMS (especially ABR) reduced the accumulation and bioavailability of HMs in soil, which in turn limited the transfer of HMs to crops, resulting in lowered human health risk indices., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)

Currently, large quantities of spent mushroom substrate (SMS) are produced annually. Because SMS has high water retention and nutrients, it has great potential to replace traditional topsoil for raising seedlings in agricultural production. However, few studies have examined the effects of substituting SMS for paddy soil on rice seedling growth and soil nutrients. SMS was mixed with rice soil in different proportions (20%, 50%, and 80%), and chemical fertilizer, organic fertilizer, and peat substrate were added in addition to equivalent nitrogen as a traditional seedling nursery method for comparison. Compared to traditional paddy soil (CK), the seedling qualities of the three SMS ratio treatments were all higher. Adding SMS at different ratios promoted rice seedling root growth, elevated the soluble protein concentration, and amplified the superoxide dismutase (SOD) enzymatic action in rice seedlings. Total porosity and aeration porosity of the soil increased by 17.40% and 32.90%, respectively. Soil organic carbon (SOC), total nitrogen (TN), and total phosphorus (TP) increased by 21.26-118.48%, 50.44-71.68%, and 23.08-80.17%, respectively. Besides, the relative abundance of Bacillus, Bacteroidetes, and other bacteria as well as the abundance of Ascomycota were all significantly increased. Adding 50% SMS increased the abundance of Pseudomonas by 8.42 times. The seedling quality of the 50% SMS treatment was even higher than chemical fertilizer and organic fertilizer treatments, only second to the peat substrate treatment. In summary, partial substitution of paddy soil with SMS can ameliorate substrate properties, improve seedling quality, and increase microbial diversity, indicating the suitability of SMS as a replacement for rice soil in seedling substrates. The 50% SMS ratio is the best. This study provides a basis for SMS to replace traditional rice soil in seedling cultivation., (© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Cultivation of Stropharia rugosoannulata with straw in forestland is effective for straw biodegradation and can prevent the waste of straw resources and environmental pollution and generate economic benefits. However, there is a lack of systematic evaluation of spent mushroom substrate (SMS) input into forestland, such as soil properties and microbial succession. In this experiment, 0 (CK), 10 (SA), 20 (SB), 30 (SC), 40 (SD), and 50 (SE) kg/m 2 straw were used to cultivate S. rugosoannulata, and two soil layers (0-10 cm, 10-20 cm) of the cultivated forestland were analyzed. The results indicated that SMS significantly promoted nutrient accumulation in forestland. The bacterial alpha diversity in the SC treatment group was greater than that in the control and gradually decreased to the control level with interannual changes, while the trend of fungal alpha diversity was opposite to that of bacterial alpha diversity. Furthermore, the SC treatment group positively affected soil nitrogen metabolism-related microorganisms for two consecutive years and significantly promoted tree growth. Habitat niche breadth and null model analysis revealed that bacterial communities were more sensitive than fungal communities after SMS input. Linear mixed model (LMM) analysis revealed that SMS supplementation significantly positively affected bacteria (Gammaproteobacteria and Bacteroidota) and significantly negatively affected fungi (Coniochaetales). The constructed fungal-bacterial co-occurrence networks exhibited modularity, and the five types of bacteria were significantly correlated with soil organic matter (SOM), soil organic carbon (SOC), available potassium (AK), available phosphorus (AAP) and available nitrogen (AN) levels. The structural equation model (SEM) showed that bacterial diversity responded more to changes in soil nutrients than did fungal diversity. Overall, 30 kg/m 2 of straw decomposition and 2 years of continuous cultivation were beneficial to soil health. This study provides new insights into the rational decomposition of straw and maintenance of forestland ecological balance by S. rugosoannulata., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have influenced the work reported in this paper., (Copyright © 2024 Elsevier B.V. All rights reserved.)

Addressing global energy demand, researchers sought eco-friendly biobutanol production from lignocellulosic waste biomass. In the present research work, five different pre-treatment methods viz., Microwave, Ultrasound, Alkali, Acid, and Hybrid, were investigated to explore its biobutanol production potential by utilizing Pleurotus ostreatus spent as substrate. The compositional and physico-chemical changes of the pre-treated Spent Mushroom Substrate (SMS) were assessed using SEM, FTIR, and XRD. Hybrid pre-treatment (Microwave, Alkali, Ultrasound) showed higher delignification when compared to conventional pre-treatment method. Hybrid pre-treated SMS resulted in higher total reducing sugars (521.53 ± 1.84 mg/g) than indigenous SMS (267.89 ± 1.53 mg/g). Fermentation of hybrid pre-treated SMS with Clostridium acetobutylicum MTCC 11274 produced the highest biobutanol concentration (9.84 ± 0.03 g/L) and yielded 0.38 ± 0.02 g/g of biobutanol. This study revealed that hybrid pre-treatment could be a promising solution for enhanced biobutanol production using SMS biomass., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Regulating nitrogen source composition is efficient approach to accelerate the spent mushroom substrate (SMS) composting process. However, currently, most traditional composting study only focuses on total C/N ratio of initial composting material. Rarely research concerns the effect of carbon or nitrogen components at different degradable level and their corresponding decomposed-substances on humification process. This study deciphers and compares the mechanism of mixed manure-N sources on SMS humification from bioavailability and molecular perspective. Two different biodegradable manure-N sources, cattle manure (CM) and Hainan chicken manure (CH), were added into the SMS composting with the different CM:CH ratio of 1:0, 3:1, 1:1, 1:3, and 0:1, respectively. The physicochemical properties and humic substances were determined to evaluate the compost quality. Coupling analysis of spectroscopy, fluorescence, and humic intermediate precursors were conducted to characterizing molecular formation process of humic acid (HA). The results indicated that regulating the carbon-nitrogen nutrient biodegradability of composting material by adding mixed nitrogen sources is an effective strategy to accelerate the SMS humification process. The C1H3 (CM:CH ratio of 1:3) and CH treatments obtained great physicochemical properties and the highest growth rate of HA (31.96% and 27.02%, respectively). The rapid reaction of polysaccharide, ketone, quinone, and amide in DOM (LCP1) might be the key for the fast humification in C1H3 and CH. The polyphenol, reducing sugar and amino acid originated from the labile-carbon-proportion I (LCP1) and recalcitrant-carbon-proportion (RCP), labile-carbon-proportion II (LCP2) and RCP, and labile-nitrogen-proportion I (LNP1), respectively, were the main driving intermediate precursors for the formation of HA. This study deciphers the SMS humification mechanism at molecular level and provides a strategy in accelerating-regulating the composting process. which will be beneficial for enhancing the disposing efficiency of SMS, producing high-quality organic fertilizer, and even popularizing to the similar types of organic waste in practical field., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Lignocellulose biomasses (LCB), including spent mushroom substrate (SMS), pose environmental challenges if not properly managed. At the same time, these renewable resources hold immense potential for biofuel and chemicals production. With the mushroom market growth expected to amplify SMS quantities, repurposing or disposal strategies are critical. This study explores the use of SMS for cultivating microbial communities to produce carbohydrate-active enzymes (CAZymes). Addressing a research gap in using anaerobic digesters for enriching microbiomes feeding on SMS, this study investigates microbial diversity and secreted CAZymes under varied temperatures (37 °C, 50 °C, and 70 °C) and substrates (SMS as well as pure carboxymethylcellulose, and xylan). Enriched microbiomes demonstrated temperature-dependent preferences for cellulose, hemicellulose, and lignin degradation, supported by thermal and elemental analyses. Enzyme assays confirmed lignocellulolytic enzyme secretion correlating with substrate degradation trends. Notably, thermogravimetric analysis (TGA), coupled with differential scanning calorimetry (TGA-DSC), emerged as a rapid approach for saccharification potential determination of LCB. Microbiomes isolated at mesophilic temperature secreted thermophilic hemicellulases exhibiting robust stability and superior enzymatic activity compared to commercial enzymes, aligning with biorefinery conditions. PCR-DGGE and metagenomic analyses showcased dynamic shifts in microbiome composition and functional potential based on environmental conditions, impacting CAZyme abundance and diversity. The meta-functional analysis emphasised the role of CAZymes in biomass transformation, indicating microbial strategies for lignocellulose degradation. Temperature and substrate specificity influenced the degradative potential, highlighting the complexity of environmental-microbial interactions. This study demonstrates a temperature-driven microbial selection for lignocellulose degradation, unveiling thermophilic xylanases with industrial promise. Insights gained contribute to optimizing enzyme production and formulating efficient biomass conversion strategies. Understanding microbial consortia responses to temperature and substrate variations elucidates bioconversion dynamics, emphasizing tailored strategies for harnessing their biotechnological potential.

Selenium nanoparticles (SeNPs) have gained significant attention in the agricultural field due to their favorable bioavailability and low toxicity, making them a highly researched subject. In this study, crude polysaccharides from spent mushroom substrate of Agrocybe aegerita (AaPs) were extracted for preparing the polysaccharide‑selenium-nanoparticles (AaPs-SeNPs) by ascorbic acid reduction method. The structure of AaPs-SeNPs was analyzed and their growth-promoting effects on rice seedlings were studied by adopting different application methods. The results revealed that AaPs-SeNPs exhibited improved free radical scavenging ability, with a lower half-maximal inhibitory concentrations compared to AaPs. Rice seedlings treated with AaPs-SeNPs showed significant enhancements in growth characteristics when compared to AaPs treatment, and foliar application exhibited a better growth-promoting effect compared to root application. Moreover, the growth performance and antioxidant enzyme activities of rice seedlings were enhanced by the addition of AaPs-SeNPs, and the absorption efficiency of essential nutrients such as N/P/K and Fe/Zn/Mn was also improved at appropriate concentrations, which could be one of the key factors contributing to the improved growth performance of plants. This study provides new aspects for the utilization of SMS, and also offers new insights from the perspective of nutrient absorption on how polysaccharide-conjugated selenium nanoparticles enhance crop growth., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier B.V. All rights reserved.)

Nowadays, agricultural by-product disposal is a major concern. The mushroom by-products could be used as an alternative feed source in rabbit diets. Therefore, partial replacement of berseem hay (BH) with spent mushroom substrate (SMS) was conducted in four experimental groups as follows: 0, 20, 40, and 60% of SMS. Forty weaned New Zealand White (NZW) rabbits at 6 th weeks of age with an initial body weight of 520.25 ± 70.01 g were fed the experimental diet for 8 weeks. The results showed that ash content and cell wall constituents in SMS were higher than in BH, while the other nutrient compounds in SMS were lower than in BH. Dietary SMS at a level of 60% increased the final body weight ( p  = 0.05) and feed conversion ratio ( p ≤ 0.05). However, average daily gain and total feed intake were not affected by treatments. The replacement of SMS at levels of 40 and 60% significantly improved nutrient digestibility and total digestible nutrient value. SMS 60% decreased ( p  = 0.040) faecal N and improved ( p  = 0.006) retained nitrogen. The SMS replacement increased caecum length ( p  = 0.001), and full and empty caecum weight ( p  = 0.001 and 0.021, respectively) compared to the control. The SMS inclusion caused a decrease ( p  = 0.021 and 0.007) in the pH and NH 3 -N concentrations, respectively. Total VFA, acetic acid, butyric acid, and propionic acid proportions increased with the dietary inclusion of SMS in a level-dependent manner. Using SMS as a replacement for BH in growing rabbit diets reduced the total feed cost, and consequently improved net revenue, economic efficiency, and relative economic efficiency.

Spent mushroom substrate (SMS) is the residual biomass generated after harvesting the fruitbodies of edible/medicinal fungi. Disposal of SMS, the main by-product of the mushroom cultivation process, often leads to serious environmental problems and is financially demanding. Efficient recycling and valorization of SMS are crucial for the sustainable development of the mushroom industry in the frame of the circular economy principles. The physical properties and chemical composition of SMS are a solid fundament for developing several applications, and recent literature shows an increasing research interest in exploiting that inherent potential. This review provides a thorough outlook on SMS exploitation possibilities and discusses critically recent findings related to specific applications in plant and mushroom cultivation, animal husbandry, and recovery of enzymes and bioactive compounds.

Using cultivated soils for rice seedlings can reduce the sustainability of arable land and thus giving negative impacts to food production. As a substitute, spent mushroom compost (SMC), which has high water-holding capacity and nutrient content, shows great potentials. To determine the impacts of the proportion of SMC and paddy soil on seedling quality, rhizosphere microbial characteristics, and fungal pathogens in rice seedling substrates, we conducted a 21-day pot experiment for rice seedling under five treatments: CK, 100% paddy soil; R1, 20% SMC and 80% paddy soil; R2, 50% SMC and 50% paddy soil; R3, 80% SMC and 20% paddy soil; and R4, 100% SMC. The results showed that incorporating SMC into the substrate, especially at 50% volume (R2), increased seedling growth and vitality at the seedling growth stage without external fertilization. Moreover, the SMC amendment increased microbial activity and promoted rice seedling recruitment of plant growth-promoting rhizobacteria (PGPR) and fungi (PGPF). In addition, using SMC significantly reduced the abundance of pathogenic fungi, especially Magnaporthe grisea. Overall, the multi-faceted benefits exhibit the strong possibilities of using SMC in sustainable rice productions., (© 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

The extraction of bioactive compounds and cellulose saccharification are potential directions for the valorization of spent mushroom substrate (SMS). Therefore, investigating the suitability of different extraction methods for recovering bioactive compounds from SMS and how the extraction affects the enzymatic saccharification is of uppermost relevance. In this work, bioactive compounds were extracted from Pleurotus spp. SMS using four extraction methods. For Soxhlet extraction (SoE), a 40:60 ethanol/water mixture gave the highest extraction efficiency (EE) (69.9-71.1%) among the seven solvent systems assayed. Reflux extraction with 40:60 ethanol/water increased the extraction yield and EE compared to SoE. A shorter reflux time yielded a higher extraction of carbohydrates than SoE, while a longer time was more effective for extracting phenolics. The extracts from 240 min of reflux had comparable antioxidant activity (0.3-0.5 mM GAE) with that achieved for SoE. Ultrasound-assisted extraction (UAE) at 65 °C for 60 min allowed an EE (~82%) higher than that achieved by either reflux for up to 150 min or SoE. Subcritical water extraction (SWE) at 150 °C resulted in the best extraction parameters among all the tested methods. Vanillic acid and chlorogenic acid were the primary phenolic acids identified in the extracts. A good correlation between the concentration of caffeic acid and the antioxidant activity of the extracts was found. Saccharification tests revealed an enhancement of the enzymatic digestibility of SMS cellulose after the extraction of bioactive compounds. The findings of this initial study provide indications on new research directions for maximizing the recovery of bioactive compounds and fermentable sugars from SMS.

The objective of this study was to investigate the microbial mechanisms for the improvement of composting efficiency after Bacillus subtilis inoculation with soluble phosphorus function in the spent mushroom substrate (SMS) aerobic composting. The methods in this study, including redundant analysis (RDA), co-occurrence network analyze and Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt 2) were carried out studying the dynamic changes of phosphorus (P) components, microbial interactions and metabolic characteristics in the SMS aerobic composting inoculated with phosphorus-solubilizing B. subtilis (PSB). An increase in germination index (GI) (up to 88.4%), total nitrogen (TN) (16.6 g kg -1 ), available P content (0.34 g kg -1 ) and total P (TP) content (3.20 g kg -1 ) and a decrease in total organic carbon (TOC), C/N and electrical conductivity (EC) in final composting stage indicated B. subtilis inoculation could further improve maturity quality of the composting product compared with CK. Other results also demonstrated that PSB inoculation increased the stability of compost, humification degree and bacterial diversity, contributing to P fractions transformation in the composting process. Co-occurrence analysis suggested that PSB strengthened microbial interactions. Metabolic function of bacterial community analysis showed pathways such as carbohydrate metabolism, and amino acid metabolism in the composting were increased by effects of PSB inoculation. In summary, this study reveals a useful basis for better regulating the P nutrient level of the SMS composting and reducing environmental risks by inoculating B. subtilis with P solubilizing function., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

Background: Carbon and nitrogen are essential energy and nutrient substances in the composting process. Corn steep liquor (CSL) is rich in soluble carbon and nitrogen nutrients and active substances and is widely used in the biological industry. Nonetheless, limited research has been done on the effect of CSL on composting. This work firstly reveals the effect of adding CSL to bacterial community composition and carbon and nitrogen conversion during composting. This study provides the choice of auxiliary materials for the spent mushroom substrate compost (SMS) and some novel knowledge about the effect of bacterial community on C and N cycling during composting of SMS and CSL. Two treatments were set up in the experiment: 100% spent mushroom substrate (SMS) as CK and SMS + 0.5% CSL (v/v) as CP., Results: The results showed that the addition of CSL enhanced the initial carbon and nitrogen content of the compost, altered the bacterial community structure, and increased the bacterial diversity and relative abundance, which might be beneficial to the conversion and retention of carbon and nitrogen in the composting process. In this paper, network analysis was used to screen the core bacteria involved in carbon and nitrogen conversion. In the CP network, the core bacteria were divided into two categories, synthesizing and degrading bacteria, and there were more synthesizing bacteria than degrading bacteria, so the degradation and synthesis of organic matter were carried out simultaneously, while only degrading bacteria were found in the CK network. Functional prediction by Faprotax identified 53 groups of functional bacteria, among which 20 (76.68% abundance) and 14 (13.15% abundance) groups of functional bacteria were related to carbon and nitrogen conversion, respectively. Adding CSL stimulated the compensatory effect of core and functional bacteria, enhanced the carbon and nitrogen transformation ability, stimulated the activity of low-abundance bacteria, and reduced the competitive relationship between the bacterial groups. This may be why the addition of CSL accelerated the organic matter degradation and increased carbon and nitrogen preservation., Conclusions: These findings indicate that the addition of CSL promoted the cycling and preservation of carbon and nitrogen in the SMS composts, and the addition of CSL to the compost may be an effective way to dispose of agricultural waste., (© 2023. The Author(s).)

Spent mushroom substrate (SMS) is a promising alternative for supplementing oyster mushroom substrate, replacing conventional cereal bran. Therefore, the objective was to evaluate the production of Pleurotus ostreatus supplemented with Lentinula edodes' SMS, through the nutritional analysis of the substrate. Wheat straw was used as substrate and supplemented with rice bran (RB) or SMS in 0%, 7%,15% and 30%. Ca, K, Mg, Mn, Zn, Cu and Fe contents of the cultivation substrates (before and after harvest) were determined through atomic absorption spectrophotometry. Mycelial growth (cm²/day), mycelial time colonization (days), number of clusters, number of pileus, average clusters weight (g), pileus lenght (cm) and width (cm), productivity (1st, 2nd and 3rd flush) (%), biological efficiency (%) of mushrooms were evaluated. Results indicated mycelial growth was higher (0.87 cm²/day compared to the Control) when the substrate was supplemented regardless of the source. The proportions of 15% of SMS achieved the highest biological efficiency (107% - 15% SMS versus 66% - Control). The only nutrients that showed differences in absorption were Ca, K and Mn, in which substrates supplemented with SMS had greater absorption of Ca (5.37 g.kg - 1 versus 1.94 g.kg - 1 in Control) while substrates supplemented with RB absorbed more K (6.56 g.kg - 1 versus 3.74 g.kg - 1 in Control). The mineral composition of the substrate has a direct impact on the growth and yield of P. ostreatus, highlighting the potential of SMS as a alternative to traditional bran supplementation., (© 2023. The Author(s), under exclusive licence to Springer Nature B.V.)

This study evaluated the adsorption and enzymatic degradation of azo dyes when using SMS. The laccase present in the SMS was characterised, and the maximum activity was obtained at pH 2, a temperature of 45°C, a Michaelis-Menten constant (K m ) of 0.264 mM, and a maximum reaction rate (V max ) of 117.95 µmol L -1 min -1 . The presence of NaCl at 5 mM inhibited enzyme activity while no inhibition was observed by Na 2 SO 4 , typically found in textile wastewater. The maximum dye adsorption (57.22%) was achieved at pH 8.0, 25°C, and 100 g L -1 of SMS while the maximum enzymatic degradation (14.18%) was obtained under the same conditions, except at pH 4.0. The enzymes laccase, lignin peroxidase, and manganese peroxidase trapped in the SMS resulted in higher dye discolouration when compared to that extracted with aqueous solution, meaning that SMS has strong adsorption capacity and is a natural immobilisation matrix, which improves the enzymatic degradation of the dyes. Thus, SMS can be used in the treatment of textile effluents for dye removal by simultaneous mechanisms of adsorption and enzymatic degradation, with reduction of environmental impacts for SMS disposal and reduction of the costs associated with commercial enzymes and adsorbents.

The changes of physicochemical and biochemical parameters of a silty loam (S1) and sandy loam (S2) vineyard soils added with spent mushroom substrate (SMS) or SMS composted with ophite (OF) as rock dust (SMS + OF) were studied. Two doses of SMS or SMS + OF (25 and 100 Mg ha -1 ) were applied for two consecutive years (2020-2021) and changes of soil physicochemical parameters, and dehydrogenase activity (DHA), respiration (RES), microbial biomass (BIO), and the phospholipid fatty acids (PLFAs) profile were assayed on a temporal basis. The results showed an increase in soil organic carbon (OC) content, total and mineralised N, P, and K, especially when the highest SMS dose was applied to soils. Repeated application caused OC content over time up to 2.3 times higher than initial content in the silty loam soil. This increase was not observed in sandy soil, possibly due to a higher bioavailability of OC, as indicated by the evolution of extractable humic acid/fulvic acid pools. In both soils, all biochemical parameters increased after amendment, being favoured both by the OC and by the presence of OF. Significant positive correlations were found between DHA, RES and BIO, and OC content especially in the first part and then levelled off after the second dose application. Total bacterial or fungal PLFAs patterns reflected the variation of BIO by SMS application. The higher growth of fungi vs. bacterial community in amended soils was recorded after the first SMS application, although the opposite effect occurred after the second application, with similar results in both soils. The findings indicate that the application of SMS or SMS + OF in vineyard soils could be an appropriate agronomic management practice for maintaining soil sustainability, although doses and application times of these amendments should first be evaluated depending on soil texture., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 The Authors. Published by Elsevier Inc. All rights reserved.)

Hydrothermal carbonization (HTC) has been recognized as a promising sewage sludge (SS) treatment technology for effective pathogen elimination, bioenergy recovery, organic contaminant destruction and volume reduction. However, the solid product (hydrochar) of SS after HTC as fuel has the problems of high ash content, high nitrogen content and low calorific value. The aqueous phase (AP) produced is still considered a burden and has become a bottleneck in the development of HTC. In this study, co-HTC of SS with spent mushroom substrate (SMS) is conducted, and the fuel properties of hydrochar and the quality of the AP as a liquid fertilizer are investigated. In comparison with hydrochar of single SS, the energy yield and higher heating value of the hydrochar from co-HTC were significantly increased by 12.1-44.8 % and 33.2-137.8 %, respectively, reaching their maximum of 72.75 % and14.98 MJ/kg, respectively. Co-HTC can improve safe handling, storage and transportation, and combustion performance of hydrochar. Furthermore, the AP of co-HTC could significantly increase the biomass of pakchoi, which was 140.9 % and 90.7 % of AP from single SS and Hoagland nutrition solution (represents commercial fertilizer), respectively. The AP of co-HTC as fertilizer can recover 62.03-64.65 % nitrogen from SS and SMS. These findings suggest that co-HTC of SMS with SS is a promising technology for the conversion SS into renewable resource products for fuels and N-rich liquid fertilizer while also significantly improving fuel and fertilizer quality., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The performance of garden waste on spent mushroom substrate (SMS) and chicken manure (CM) co-composting efficiency and humification is unclear. Therefore, this study investigated the impact of garden waste addition on SMS-CM co-composting physicochemical properties, humification process, and the spectral characteristics of dissolved organic matter (DOM). The results showed that garden waste improved the physicochemical properties of SMS-CM co-compost, the thermophilic period was advanced 2 days, the seed germination index increased by 30.2%, and the total organic carbon and total nitrogen content increased by 8.80% and 15.0%, respectively. In addition, garden waste increased humic substances (HS) and humic acid (HA) contents by 10.62% and 34.52%, respectively; the HI, PHA and DP increased by 31.53%, 43.19% and 55.53%, respectively; and the SUVA 254 and SUVA 280 of DOM also increased by 6.39% and 4.39%, respectively. In particular, HA content and DOM humification increase rapidly in the first 10 days. The increase of HA accounted for 52% of the total increase during composting. Fourier-transform infrared and two-dimensional correlation analysis further confirmed that garden waste could facilitate the degradation of organic molecules, including amino acids, polysaccharides, carboxyl groups, phenols, and alcohol, and contributed to the preferential utilization of carboxyl groups and polysaccharides and thus enhanced humification., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Bio-enrichment of edible mushrooms is an outstanding strategy to deliver essential nutrients to human. In this study, an edible fungus; Pleurotus pulmonarius was cultivated on spent mushroom substrate (SMS) supplemented with copper, lithium, and zinc. Proximate and mineral analysis of cultivated mushroom was determined using methods of AOAC. Antimicrobial activity of cultivated mushroom was assessed against microorganisms using agar well diffusion. Antioxidant property of mushroom was assessed against free radicals. Similar (p ≤ 0.05) protein contents of 18.93%, 18.80% and 17.90% were respectively obtained in P. pulmonarius biofortified with Cu + Li + Zn, Cu + Zn and Zn. Crude fibre in element fortified-mushroom ranged from 9.02 to 10.11%, while non-fortified mushroom was 8.66%. Copper content of P. pulmonarius fortified with Cu alone and Cu + Zn were 96.12 mg/100 g and 98.09 mg/100 g, respectively. Mushroom fortified with Zn has the highest zinc content of 520.15 mg/100 g. Mushroom fortified with Li and Li + Zn have a similar (p ≤ 0.05) Li content of 106.02 mg/100 g and 104.30 mg/100 g, respectively. Extract from mushroom-fortified with copper has the highest zone of inhibition (15.1 mm) against Klebsiella pneumoniae at 1.0 mg/ml. Mushroom fortified with Cu + Li + Zn and Li + Zn, respectively have similar (p ≤ 0.05) scavenging activities of 79.10 and 81.0% against DPPH. Mushroom fortified with Zn or Zn + Cu enhanced the growth of Lactobacillus acidophilus and Lactobacillus plantarum. Antimicrobial, antioxidant and prebiotic activities of fortified-mushroom could be attributed to arrays of phytochemicals and bio-accumulated elements. Hence, bio-fortified mushrooms can be used as functional foods and as biopharmaceuticals to treat ailments associated with nutrient deficient., (© 2022. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.)

Two composting experiments were conducted to investigate the effects of commercial microbial agents on microbial succession and nutrient flow such as humification, maturation, and stability during the aerobic composting of the spent mushroom substrate (SMS). The cellulose degradation rate of T (added microbial agents at the initial stage) reached 41.8%, which was much significantly (p < 0.05) higher than that of CK (14.9%). The seed germination index (GI) in T (82.38%) was significantly (p < 0.05) higher than that in CK (74.74%) in the maturation phase. Moreover, the total organic carbon/total nitrogen ratio (C/N) and electrical conductivity (EC) value of T decreased to 10.5 and 2.37 mS/cm, respectively. Chemical detection and fluorescence excitation-emission region integration method (EEM-FRI) analysis showed that the microbial agents significantly accelerated the organic matter (OM) decomposition and promoted the quality of mature compost using SMS as a single raw material. The bacterial abundance of T was significantly richer than the CK due to the addition of microbial agents. The results could provide a comprehensive understanding of adding microbial agents into composting SMS and a scientific feasibility strategy to rational utilization of resources in the edible fungi industry, which was conducive to the waste management and sustainable development of the edible fungi industry., (© 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

In recent years, enormous amounts of spent mushroom substrate (SMS) have been generated because of the rapid development of mushroom production. Since the conventional disposal methods of these residues can cause serious environmental problems, alternative waste management techniques are required to ensure sustainable agriculture. However, SMS might be not suitable for vermicomposting when used alone. Therefore, the primary purpose of this study was to investigate the effect of Azolla microphylla (Azolla) biomass, eggshells, fruit peels, and cassava pulp on the biodegradation process of SMS. The results showed the treatments supplemented with cassava pulp and fruit peel waste improved the growth of earthworms, while the carbon-to-nitrogen ratio of these vermicomposts decreased significantly (p < 0.05) due to the improved total nitrogen contents (7.64 g kg -1 and 6.71 g kg -1 ). Concerning the degradation process and the vermicompost quality, the addition of these agro-residues facilitated the enzyme activities (cellulase, urease, and alkaline phosphatase) and increased the total macronutrient (P, K, Mg, and Ca) and phytohormone (fruit peel waste: AA, GA3, and cytokinin; cassava pulp: cytokinin) contents of the final products compared to the control treatment. On the other hand, Azolla had no additional effect on the fecundity and growth of Eudrilus eugenia. Meanwhile, the treatment supplemented with eggshells was high in Mg (7.15 g kg -1 ) and Ca (305.6 g kg -1 ). Overall, the combined decomposition of SMS-based bedding material with Azolla, eggshells, fruit peel waste, and cassava pulp resulted in mature organic fertilizers with improved chemical properties., (Copyright © 2022. Published by Elsevier Inc.)

A calcium-pyro-hydrochar (Ca-PHC) can be distinguished as a novel sorbent of Pb 2+  and Cd 2+  from an aqueous solution. It was obtained using hydrothermal treatment of the spent mushroom substrate (SMS), followed by a CaCl 2 ·5H 2 O activation and pyrolysis. The characterisation of chars before and after modifications was done by scanning electron microscope (SEM), Brunauer-Emmett-Teller (BET) and Fourier transform infrared (FTIR). Batch experiments were performed to examine Ca-PHC's sorption properties and binding mechanisms to selected metal ions. The maximum sorption capacities of Ca-PHC for Pb 2+  and Cd 2+  were 297 mg g -1 , and 131 mg g -1 , respectively. The obtained results demonstrated that the sorption of Pb 2+  and Cd 2+  by Ca-PHC follows a pseudo-second kinetic model and Freundlich isotherm. The binding of the selected metals onto Ca-PHC was enabled by the ion-exchange mechanism, surface complexation, mineral precipitation and cation-π interaction. Thermodynamic parameters indicate that metal ions binding by Ca-PHC are spontaneous and endothermic. Due to the high adsorption capacities, the obtained Ca-PHC has good potential for application in industrial wastewater treatment. In addition, the demonstrated use of SMS highlights another possibility of applying this specific biomass relevant to sustainable and economical waste management in the growing mushroom industry.

Due to the nutritional values and functional bioactivities of mushrooms, the global market value of the edible mushroom industry has been growing steadily. However, the production of 1 kg of fresh mushroom generates about 5 kg of wet byproducts (known as spent mushroom substrate; SMS). This necessitates proper waste management to mitigate potential environmental threats. Embracing the "waste-to-fuel" concept, SMS as lignocellulosic waste can serve as cheap and abundant feedstock for the production of a variety of biofuels, including biogas, biohydrogen, bioethanol, bio-oil, and solid-biofuels. Mushroom cultivation serves as efficient biological pretreatment for biofuel production, promoting biofuel yield and improving the overall economy. Therefore, integrated mushroom cultivation and biofuel production can simultaneously satisfy the rapidly rising food and energy demand. The article systematically reviewed the recycling and re-utilization of SMS in sustainable biofuel production, discussing the possible challenges and proposing future directions for the green development of the mushroom industry., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

The huge volumes of currently generated agricultural waste pose a challenge to the economy of the 21st century. One of the directions for their reuse may be as fertilizer. Spent mushroom substrate (SMS) could become an alternative to manure (M). A three-year field experiment was carried out, in which the purpose was to test and compare the effect of SMS alone, as well as in multiple variants with mineral fertilization, and in manure with a variety of soil quality indices-such as enzymatic activity, soil phytotoxicity, and greenhouse gas emissions, i.e., CO 2 . The use of SMS resulted in significant stimulation of respiratory and dehydrogenase activity. Inhibition of acid phosphatase and arylsulfatase activity via SMS was recorded. SMS showed varying effects on soil phytotoxicity, dependent on time. A positive effect was noted for the growth index (GI), while inhibition of root growth was observed in the first two years of the experiment. The effect of M on soil respiratory and dehydrogenase activity was significantly weaker compared to SMS. Therefore, M is a safer fertilizer as it does not cause a significant persistent increase in CO 2 emissions. Changes in the phytotoxicity parameters of the soil fertilized with manure, however, showed a similar trend as in the soil fertilized with SMS.

Aerobic composting is increasingly regarded as a promising technology for the recycling of spent mushroom substrate (SMS), and an applicable nitrogen source is necessary to improve the process. This study is the first to investigate the effects of protein-like N source (chicken manure, CM) and high-N source (urea, UR) on humification process and P dynamics during SMS composting. The effect of different N sources on microbial succession was also studied. Results showed that CM addition achieved a longer thermophilic phase (16 d vs 9 d), greater germination indices (131.6% vs 106.3%), and higher total phosphorus content (13.1 g/kg vs 6.56 g/kg) in the end products, as compared to UR. The addition of CM showed beneficial effects on humification and stabilization, including decreased weight loss and fluctuations in the level of functional groups. The P produced in the compost was interconverted and leached in the P pool. In this case, the P detected in the compost was in the form of orthophosphate and MgNH 4 PO 4 ⋅6H 2 O crystal as inorganic P and orthophosphate monoester as organic P. The most abundant microorganisms at the phylum level mainly include Firmicutes, Actinobacteria, and Proteobacteria, accounting for more than 88% of the total microorganisms. The addition of CM to SMS compost resulted in higher organic matter degradation rates. This work clarified the role of various N sources in SMS composting and presented an appropriate waste management method beneficial to bioresource technology and sustainable development of the edible fungi business., (Copyright © 2022 Elsevier Ltd. All rights reserved.)

Some studies have reported the method for treating the spent mushroom substrate (SMS). However, the effective use as a functional raw material based on properties of SMS remains a formidable challenge. In this study, we investigated the usefulness of SMS in agriculture to develop a new method for treating and utilizing it. First, we attempted to isolate chitin/cellulose nanofiber complex (CCNFC) from SMS using chemical pretreatment and mechanical fibrillation. The characterization results like SEM, FT-IR, and XRD showed that we successfully isolated the CCNFC from SMS. Second, we explored the biological activities of the CCNFC for its potential application as a functional agricultural nanomaterial. CCNFC water dispersion with low concentration (0.1 and 1 mg/mL) exhibited significant plant disease resistance and plant growth promotion activities. Our results suggested that SMS may provide a useful source of functional agricultural nanomaterial, which may contribute to treating and applying it in agriculture., (Copyright © 2022 The Authors. Published by Elsevier Ltd.. All rights reserved.)

Poly(ε-L-lysine) and poly(L-diaminopropionic acid) are valuable homopoly (amino acids) with antimicrobial properties and mainly produced in submerged fermentation. In this study, we investigated their co-production using waste biomass and spent mushroom substrate in solid-state fermentation. Simultaneous production of poly(L-diaminopropionic acid) and poly(ε-L-lysine) was achieved in a single fermentation process using pearl oyster mushroom residues as substrate, with the supplement of glycerol and corn steep liquor. After optimization of the fermentation parameters, the maximum yield of poly(ε-L-lysine) and poly(L-diaminopropionic acid) reached 51.4 mg/g substrate and 25.4 mg/g substrate, respectively. The optimal fermentation conditions were 70% initial moisture content, pH of 6.5, 30°C and an inoculum size of 14%. Furthermore, the fermentation time was reduced from 8 days to 6 days using repeated-batch solid-state fermentation. Finally, the antimicrobial effects of poly(L-diaminopropionic acid) and poly(ε-L-lysine) were evaluated in freshly pressed grape juice, which indicated tremendous potential of this mixture in its use as biological preservative.

Commercial mushrooms are cultivated on lignocellulose wastes, such as corncob, saw dust, straw and wood chips. Following the rapidly increasing global mushroom production, the efficient recycling and utilization of the by-product, known as spent mushroom substrate (SMS) has garnered much attention due to the serious pollution issues caused. Embracing the concept of 'circular economy', the SMSs have demonstrated immense potential in wide range of applications, including recycling as the substrate for new cultivation cycle of mushroom, biofertilizer and soil amendment, animal feed, renewable energy production and pollution bioremediation. The review provided an overview and recent advances focusing on these applications, analyzed the possible challenges and proposed future directions for sustainable development of global mushroom industry., (Copyright © 2021. Published by Elsevier Ltd.)

The present investigation was performed to valorize paddy straw (PS) based silica (Si) rich Spent Mushroom Substrate (SMS) of Pleurotus ostreatus for Plant Nutrient and Disease Management in wilt (caused by F. oxysporum f. sp. lycopersici) susceptible tomato plant F1 Hybrid King 180. Raw PS and SMS generated by P. ostreatus cultivated on PS only, and PS amended with 5% soybean cake (SC) were bio-fortified with Trichoderma asperellum (TA). SMS (PS+ 5% SC) was found supporting the growth of T. asperellum to an extent of 12.37 × 10 13 conidia/g substrate. GC-MS analysis of SMS detected several bioactive metabolites like Palmitic acid, Oleic acid, Methyl linoleate, Stigmasterol, etc., known for plant health management. Bioformulations were developed employing Press Mud (PM) and Talcum Powder (TP) as carrier materials. Among the different bioformulations tested in pots study; SMS (PS+ 5% SC) SiTAPM, collectively named as TF-I, provided improved levels of morpho-biochemical and nutritional parameters, i.e., Plant Biomass (2.27 folds), Root Volume (1.75 folds), Chlorophyll (2.66 folds), Carotenoids (2.42 folds), Number of Fruits (1.76 folds), Fruit Biomass (2.02 folds), Total Soluble Sugars (2.32 folds), Total Soluble Proteins (1.70 folds), and nutraceutical parameters as Lycopene (1.42 folds), β-carotene (2.65 folds) and Ascorbic Acid (1.54 folds), along with significant (p < 0.05) reduction in the Disease Severity Index (84.34%-21.23%), over the pathogen affected plant taken as control. The fruits and leaves garnered under TF-I displayed Total Polyphenol Content (TPC) of 74.5 and 126.9 mg g -1 gallic acid, respectively, with 83.73% DPPH and 72.25% FRAP activity, indicating the elicitation of antioxidant properties in tomato fruits. EDS analyses showed 21.53% Si in SMS, and plant mapping investigation indicated a substantial accumulation of Si, which is well conceded to promote growth, disease resistance, and antioxidant parameters. The study also endorsed the use of PM over TP, as TF-I recorded an acceptable conidial count (2.22 × 10 8  cfu/g) towards the end of six months storage period over other bioformulations. Overall, the study envisages the development and application of innovative methodology (TF-I), offering an eco-friendly alternative for producing quality crops and a sustainable solution to waste management, thus delivering a holistic contribution towards the circular economy., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

Most commercially circulating mushrooms are produced via cultivation using artificially produced mushroom substrates. However, after mushroom harvesting, the disposal of spent mushroom substrates (SMSs) is a serious problem for the mushroom industry owing to the need for a disposal site and the cost involved. Thus, in view of the possibility of recycling SMSs as a soil modifier, we examined the effect of soil mixed with SMSs on the infection of Arabidopsis leaves by Alternaria brassicicola, the causal agent of cabbage leaf spot. The mixing of SMSs used for Hypsizygus marmoreus, Pholiota microspora, Lyophyllum decastes, and Auricularia polytricha into culture soil suppressed the lesion formation caused by A. brassicicola. The defense responses of Arabidopsis were not induced by the culturing of these seedlings in soils containing SMSs. Suppressed lesion formation was observed after the seedlings were treated with volatiles emitted from SMSs that were incubated with soil for 7 days and used for H. marmoreus, P. microspora, L. decastes, A. polytricha, Lentinula edodes, and Cyclocybe cylindracea. The volatiles from the SMSs reduced the elongation of A. brassicicola hyphae. GC-MS analyses of extracts from the SMS containing soils led to the detection of various volatile compounds; among these, skatole, 2,4-di-tert-butylphenol, γ-dodecalactone, butyric acid, guaiacol, 6-amyl-2-pyrone, and 1-octen-3-ol were examined for inhibitory activity on A. brassicicola and found to suppress hyphae elongation. These findings indicate that the antifungal volatile compounds emitted by the SMSs suppress A. brassicicola infection., (Copyright © 2021 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.)

To investigate the remediation potential of spent mushroom substrate (SMS) on Cd pollution in a paddy soil, a rice pot experiment was conducted to study the effects of SMS addition on the availability of Cd in soil and the uptake of Cd in rice tissues. Five percent of SMS from Pleurotus eryngii (SMS-A, treatment: A), SMS from Agaricus bisporus (SMS-B, treatment: B), or SMS-A plus SMS-B (1:1, treatment: A+B) were added into a Cd-contaminated paddy soil before planting, respectively. The treatment of no SMS amendment was set up as the control (CK). At the four main growth stages of rice, the soils and plant samples were collected to detect the soil properties, Cd concentration in soils and rice tissues, and Cd fractions in soils. Results indicated that the application of SMS-A, SMS-B, and A+B significantly increased soil pH by 14.0-22.9, 23.9-32.9, and 22.7-30%, organic matter (OM) contents by 12.9-31.5, 22.1-34.5, and 26.1-36.9% comparing with CK. While cation exchange capacities (CECs) were increased by 3.6-8.5, 4.9-13.1, and 0.4-10.0% in A, B, and A+B treatments, respectively, except those at the maturation stage in A and B treatments. However, the CaCl 2 -Cd concentrations in soils were significantly decreased by 64.8-77.9, 76.1-98.9, 73.2-98.9% in A, B, and A+B treatments, respectively, comparing with CK. The reduced availability of Cd was attributed to the changes of Cd from soluble to insoluble fractions in soils amended with SMS and resulted in the decreased Cd uptake in rice tissues. The Cd concentrations in roots significantly decreased by 22.8-36.9, 28.6-36.6, and 26.8-42.6%, while the Cd concentrations in straw decreased by 20.1-46.4, 9.3-41.6, and 16.0-49.1% in A, B, and A+B treatments, respectively. At the maturation stage, the Cd concentrations in brown rice were reduced by 17.7, 15.9, and 19.4% in A, B, and A+B treatments, respectively. Correlation analysis revealed that the Cd concentrations in rice roots, straws, and brown rice were all positively correlated with CaCl 2 -Cd concentrations of soils. Moreover, soil pH and OM were significantly negatively correlated with the Cd concentration in rice tissues, except that between soil pH and the Cd concentration in rice straws. Therefore, the reduced Cd availability in soil and uptake in rice plant tissues together with better soil nutrient conditions by SMS application improved the biomass of root and straw at heading, filling, and maturation stages and the rice production by 32.9-38.8% at the maturation stage. The combined application of SMS-A and SMS-B can be used as a potential method for remediation of Cd-contaminated paddy soil., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.)

Plant defense responses are activated by various exogenous stimuli. We found that an aqueous extract of spent mushroom substrate used for the cultivation of Hypsizygus marmoreus induced defense responses in rice. Fractionation of the spent mushroom substrate extract indicated that the compounds responsible for this induction were neutral and hydrophilic molecules with molecular weights lower than 3 kDa. Compounds with these characteristics, namely glucose, fructose, and sucrose, were detected in the extract at concentrations of 17.4, 3.3, and 1.6 mM, respectively, and the treatment of rice leaves with these sugars induced defense responses. Furthermore, microarray analysis indicated that the genes involved in defense responses were commonly activated by the treatment of leaves with spent mushroom substrate extract and glucose. These findings indicate that the induction of defense responses by treatment with spent mushroom substrate extract is, at least in part, attributable to the sugar constituents of the extract., (© The Author(s) 2021. Published by Oxford University Press on behalf of Japan Society for Bioscience, Biotechnology, and Agrochemistry.)

Synthetic adhesives in the plywood industry are usually volatile compounds such as formaldehyde-based chemical which are costly and hazardous to health and the environment. This phenomenon promotes an interest in developing bio-boards without synthetic adhesives. This study proposed a novel application of natural mycelium produced during mushroom cultivation as natural bio-adhesive material that convert spent mushroom substrate (SMS) into high-performance bio-board material. Different types of spent mushroom substrates were compressed with specific designed mould with optimal temperature at 160 °C and 10 mPa for 20 min. The bio-board made from Ganoderma lucidum SMS had the highest internal bonding strength up to 2.51 mPa. This is far above the 0.4-0.8 range of China and US national standards. In addition, the material had high water and fire resistance, high bonding and densified structures despite free of any adhesive chemicals. These properties and the low cost one step procedure show the potential as a zero-waste economy chain for sustainable agricultural practice for waste and remediation., (Copyright © 2020 Elsevier B.V. All rights reserved.)

Spent mushroom substrate (SMS) is an agricultural waste with a high potential for polycyclic aromatic hydrocarbons (PAH) removal in aged contaminated soils. In this study, fresh and air-dried Pleurotus ostreatus, Pleurotus eryngii, and Auricularia auricular SMSs were used to remove PAHs in agricultural soil under 60-day incubation. The potential of SMS in PAH dissipation was studied by detecting the dissipation rate and the soil physicochemical index, enzyme activity, PAH-degradation bacterial biomass, and microbial diversity. Results showed that SMS significantly enhanced the dissipation of PAHs and fresh SMS had a better effect than air-dried SMS. The highest dissipation rate of 16 PAHs was 34.5%, which was observed in soil amended with fresh P. eryngii SMS, and the PAH dissipation rates with low and high molecular weights were 41.3% and 19.4%, respectively. By comparison, fresh P. eryngii SMS presented high nutrient contents, which promoted the development of PAH-degrading bacteria and changed the soil bacterial community involved in degradation, thereby promoting the PAH dissipation. The lignin-degrading enzymes in fresh SMS were abundant, and the laccase and manganese peroxidase activities in the treatment of fresh P. eryngii SMS was higher than those in other treatments. Fresh P. eryngii SMS improved the relative abundance of Microbacterium, Rhizobium, and Pseudomonas in soil, which were all related to PAH degradation. Consequently, adding fresh P. eryngii SMS was an effective method for remediating aged PAH-contaminated agricultural soils., Competing Interests: Declaration of competing interest All the authors declare that they have no conflict of interest., (Copyright © 2020 Elsevier Ltd. All rights reserved.)

Pleurotus ostreatus is one of the most cultivated mushrooms in the Czech Republic. The production of 1 kg of mushrooms generates about 5 kg of spent mushroom substrate. A gentle and fast method for using this substrate is vermicomposting. Vermicomposting of spent mushroom substrate using Eisenia andrei was conducted for seven months. For control purposes, a treatment without earthworms was also prepared. The vermicomposting process used vertical continuous feeding vermicomposters. The agrochemical and biological parameters were analysed. Values of electrical conductivity were very high in both vermicomposters (higher than 2000 μS/cm). During the vermicomposting process the C/N ratio decreased. The number and biomass of earthworms decreased with the age of the layers. The values of total P, K and Mg were higher in the vermicomposter without earthworms. There were also lower microbial phospholipid fatty acids content - than in the vermicomposter with earthworms. However, the fungal phospholipid fatty acids content were two times higher in the vermicomposter without earthworms. The highest hydrolytic enzyme activity was found in lipase, acid phosphatase and β-D-glucosidase. Most hydrolytic enzymes were more active in the vermicomposter without earthworms, with the exception of arylsulphatase. Mn-peroxidase activity was higher in the vermicomposter without earthworms and laccase activity was below the detection limit., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2020 Elsevier B.V. All rights reserved.)

This study used a laboratory-scale system to investigate the effects of calcium magnesium phosphate fertilizer (CaMgP), biochar, and spent mushroom substrate (SMS) on compost maturity and gasous emissions during pig manure composting. The results showed that the addition of CaMgP, Biochar or SMS had no negative effect on the quality and maturity of compost, and all three additives could reduce the emissions of ammonia (NH 3 ), hydrogen sulfide (H 2 S), dimethyl sulfide (Me 2 S) and dimethyl disulfide (Me 2 SS). Among them, the effect of adding CaMgP on NH 3 emission reduction was the most obvious, reduced 42.90%. The emission reduction of CaMgP to H 2 S was similar to that of SMS, which decreased by 34.91% and 32.88% respectively. The emission reduction effects of the three additives on Me 2 S and Me 2 SS were obvious, all of which were over 50%. However, only adding SMS reduced the N 2 O emission by 37.08%., Competing Interests: Declaration of competing interest None., (Copyright © 2020. Published by Elsevier Ltd.)

The edible mushroom industry produces massive amounts of spent mushroom substrate (SMS). Thus, there is an urgent need for high-value utilization technology to process the SMS, especially SMSs originating from woodchips. Protaetia brevitarsis larvae (PBL) can feed on various types of organic matter and can produce organic fertilizer and insect protein. In this study, we investigated the potential of PBL to utilize and convert SMSs from Auricularia auricula (SMS-AA) and Lentinula edodes (SMS-LE) cultivation. The results showed that the PBL were able to feed on SMS-AA and SMS-LE and form nutrient-enriched organic fertilizer with a low phytotoxicity and high humic acid content. Further analysis of the organic carbon dynamics suggested that PBL can efficiently digest and utilize lignin. This study demonstrates a new strategy for the utilization of SMSs originating from woodchips, and provides a new model for further investigations on the mechanism of lignin decomposition., Competing Interests: Declaration of Competing Interest The authors declare no commercial or financial conflict of interest., (Copyright © 2020. Published by Elsevier Ltd.)

Edible mushroom cultivation is an important industry in intensively managed forest understories. However, proper disposal of spent mushroom substrate (SMS) presents a challenge to its sustainable development. Biochar derived from SMS could be used to improve soil quality while providing a solution for SMS disposal. But SMS biochar pyrolyzed at different temperatures may alter carbon dioxide (CO 2 ) and nitrous oxide (N 2 O) emissions associated with global warming, especially under the context of nitrogen (N) addition and warming. We conducted a factorial incubation study to examine greenhouse gas emissions and N transformations in moso bamboo forest soil amended with SMS-biochar (control vs. pyrolyzed at 300, 450 or 600 °C) in different N-addition (0 or 100 mg N kg -1 soil) and temperature (20, 25 or 30 °C) treatments. Pyrolysis temperature affected pH, C and N of SMS-biochars. N-transformations depended on the interaction of pyrolysis temperature, N-addition, and incubation temperature but were generally lower with 450 °C biochar addition. Soil N 2 O emissions increased with N-addition and they were more sensitive to incubation temperatures without biochar. Soil CO 2 emissions increased with incubation temperature or biochar pyrolyzed at lower temperatures. Pyrolysis temperature might have regulated the effects of SMS-derived biochar on N 2 O emissions via changes in dissolved C, N, pH and associated changes in soil microbial community compositions. Because of the importance of sustainable development of this understory industry, amending soils with biochar produced at higher temperatures may be the best strategy for both the disposal of SMS and the mitigation of greenhouse gas emissions., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

A facultatively anaerobic, Gram-stain-positive, spore-forming Bacillus strain, 17-SMS-01 T , isolated from spent mushroom substrate in the Fangshan District, Beijing, PR China, was initially identified as a Bacillus cereus group species based on 16S rRNA gene sequences. Strain 17-SMS-01 T had the highest sequence similarities to Bacillus wiedmannii FSL W8-0169 T (99.9 %), Bacillus albus N35-10-2 T (99.9 %), Bacillus luti TD41 T (99.9 %) and Bacillus proteolyticus TD42 T (99.9 %). However, the average nucleotide identity (ANI) and digital DNA-DNA hybridization (DDH) values between strain 17-SMS-01 T and the most closely related species were less than the previously proposed cut-off values of 96 % (ANI) and 70 % (DDH) for differentiating species within the genus, suggesting that this strain represents a novel Bacillus group species. The fatty acid profile of strain 17-SMS-01 T , which showed a predominance of iso-C 15 : 0 and anteiso-C 15 : 0 , supported the allocation of the strain to the genus Bacillus . The predominant menaquinone was MK-7 (100%). The major polar lipids were diphosphatidylglycerol, phosphatidyl ethanolamine, phosphatidyl glycerol, an unidentified aminophospholiped and unidentified lipids. The DNA G+C content of the novel strain was 35.0 mol%. The results of physiological and biochemical tests also allowed the phenotypic differentiation of strain 17-SMS-01 T from the most closely related recognized species. On the basis of the phylogenetic and phenotypic evidence, strain 17-SMS-01 T represents a novel Bacillus species, for which the name Bacillus fungorum sp. nov. is proposed. Type strain of the novel species is 17-SMS-01 T (=MCCC 1K03483 T =KCTC 33949 T ).

Paracoccus sp. LXC combined with humic acid (HA) and spent mushroom substrate (SMS) obtained from Auricularia auricular and Sarcomyxa edulis was tested for the remediation of agricultural soil contaminated with aged polycyclic aromatic hydrocarbons (PAHs). The biomass and diversity of bacteria and fungi and the soil enzyme activity were analyzed. PAH removal and dissipation kinetics were examined. The highest degradation rate of PAHs was 56.5% when soil was amended with Paracoccus sp. LXC combined with HA and unsterilized SMS from A. auricular. The half-life of PAHs decreased from 2323.3 days in natural attenuation to 66.6-277.2 days in amended treatments. Soil treated with Paracoccus sp. LXC combined with HA and SMS from A. auricular acquired high contents of organic matter and nutrients. HA and SMS aided the growth of PAH-degrading bacteria and promoted the diversity of bacteria but not of fungi. The degradation rate of PAHs was mainly correlated positively with soil laccase activity. Low- and middle-molecular-weight PAHs were significantly removed by Paracoccus sp. LXC, HA and SMS. High-molecular-weight PAHs were removed by SMS but not by Paracoccus sp. LXC. Biodegradation by Paracoccus sp. LXC combined with HA and SMS is a promising choice for remediating aged PAH-contaminated agricultural soils., (Copyright © 2019 Elsevier B.V. All rights reserved.)

Garden wastes (GW) having high lignin contents could hinder the growth of earthworms and microorganisms in vermicomposting. This study investigated the Eisenia fetida-based vermicomposting of GW mixed with cattle manure (CM) and/or spent mushroom substrate (SMS) at different ratios of GW alone (control), 3:1 GW:SMS, 1:1 GW:SMS, 3:1 GW:CM, 1:1 GW:CM and 2:1:1 GW:SMS:CM to promote earthworm growth and improve the final vermicompost quality. In general, treatments with the addition of SMS and/or CM increased the survival rate, biomass, cocoon and juvenile numbers of E. fetida compared to the control. The addition of SMS and/or CM also significantly increased the activities of dehydrogenase, cellulase, urease, and alkaline phosphatase compared to the control. Furthermore, the addition of SMS and/or CM facilitated the decomposition of organic matter, cellulose and lignin, increased nutrient (N, P and K) concentrations, and accelerated nitrification compared to the control. The addition of SMS and CM led to greater chemical changes of the substrate compared to control. Heavy metal concentrations were increased in the final vermicomposts comparatively to the initial materials, but none of them exceeded the permissible limits. The highest germination index of Chinese cabbage and tomato seeds were both observed in the treatment of 2:1:1 GW:SMS:CM which reached 146.9 and 148.1. Overall, the 2:1:1 GW:SMS:CM treatment had the highest growth and reproduction rates of E. fetida, higher percentage degradation of organic matter, cellulose and lignin, as well as the best quality of the final vermicompost., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Spent mushroom substrate (SMS) and textile dyeing sludge (TDS) were (co-)combusted in changing heating rates, blend ratios and temperature. The increased blend ratio improved the ignition, burnout and comprehensive combustion indices. A comparison of theoretical and experimental thermogravimetric curves pointed to significant interactions between 350 and 600 °C. High content of Fe 2 O 3 in TDS ash may act as catalysis at a high temperature. Ignition activation energy was lower for TDS than SMS due to its low thermal stability. 40% SMS appeared to be the optimal blend ratio that significantly decreased the activation energy, as was verified by the response surface methodology. D3 model best described the (co-)combustions. SMS led to more NO and NO 2 emissions at about 300 °C and less HCN emission than did TDS. The addition of 40% SMS to TDS lowered SO 2 emission. The co-combustion of TDS and SMS appeared to enhance energy generation and emission reduction., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

The feasibility of producing biochar and its steam-activated counterpart in a large scale (1000 kg) from spent mushroom substrate (SMS) and their usage as effective environmental remediation tools to augment current SMS management strategies were explored. Steam-activated SMS biochar exhibited enhanced surface area (332 m 2 /g), pore volume (0.29 cm 3 /g), and porosity (77.1%). The effectiveness of activation was higher on the cationic dye, crystal violet (CV) by 4.1 times increase from 255 mg/g to 1057 mg/g. The biochar and its steam-activated counterpart, respectively exhibited high COD and color removal efficiencies of 49.6% and 40.1%, and 67.7 and 99.6% for CV-spiked real wastewater. Reusability studies confirmed the dominant role of chemisorption in the adsorption process. The lower production cost coupled with the superior physicochemical properties and adsorption performances rendered the biochar with/without steam activation, as a promising alternative adsorbent to serve as a green, viable and effective environmental remediation tool., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Recycling of BR and SMS are crucial for the development of biogas industry and commercial mushroom cultivation. The seed germination test is limited to examine the maturity of compost because of lacking the effect of insoluble part on plant growth. The aim of this study was to evaluate the maturity of compost by analysis the relationship between agronomic parameters of plant growth with physicochemical parameters of compost. The thermophilic period (over 50 °C) was lasted 52 days. TOC, C/N, AP and NH 4+ -N was decreased along with composting process, while TK, TP, AK and NO 3 - -N showed an opposite trend. As for seedling quality, the raw material (T0) showed the worst plant growth but the 100% compost (T1) showed better seedling quality compared with commercial seedlings. According to the analysis of Spearman correlation, the results indicated that TOC, C/N, NH 4 + -N, NO 3 - -N, AK and lignocellulose can be used to evaluate compost maturity., (Copyright © 2019 Elsevier Ltd. All rights reserved.)

Spent mushroom substrate (SMS) as an organic amendment to plant production has received increasing attention on soil phytoremediation. However, organic amendments are known to contribute to greenhouse gas (GHG) emission from soils. Castor oil plant has a high biomass production and phytoremediation potential for heavy metal-contaminated soils. In the present study, the roles of SMS on phytoremediation efficiency of castor oil plant (Ricinus communis L.) from cadmium (Cd) and nickel (Ni)-contaminated soils were investigated, and the impact of SMS application on methane emission from the contaminated soil were evaluated. Pot experiments with SMS-amended and unamended contaminated soils were conducted to investigate Cd and Ni accumulation in R. communis and CH 4 emission. After growing for 3 months in soils with the addition of Cd (10 mg/kg) and Ni (at rates of 200 and 600 mg/kg), the dry biomass and the concentrations of Cd and Ni in the R. communis were measured, and the mobility factors for Cd and Ni were calculated. To assess methane emission, CH 4 fluxes and potential rates of CH 4 production and oxidation were measured pre- and post-incubation. SMS addition significantly improved the growth of R. communis and gave 19.15~82.46% more dry weight as compared to the single plant cultivation in the contaminated soils. SMS also increased plant Cd uptake and the total amount of Cd accumulation in R. communis increased by 28.1-152.1%, respectively, in signal Cd treatment and Cd-Ni complexation treatment, as compared to the single plant cultivation. The high values of mobility factor for Cd in single plant cultivation and co-application of SMS and R. communis pointed to the potential of R. communis to the Cd mobilization from the contaminated soils. Moreover, the addition of SMS tended to stimulate CH 4 uptake that the average increases in CH 4 uptake rate were 3.84-fold (in controls) and 2.91-fold (in single Cd treated soils) by the co-application of SMS and R. communis as compared to the single plant cultivation. The results suggested that the application of SMS could improve the growth of R. communis in Cd and Ni-contaminated soil, enhance heavy metal bioaccumulation, and stimulate soil CH 4 uptake. Therefore, SMS might be useful for enhancing phytoremediation of heavy metals and mitigate CH 4 emission from the contaminated soil. In addition, results in the study implied that implementing carefully designed management strategies (e.g., application of organic residues) during contaminated soil remediation is a promising solution for agricultural waste management and soil phytoremediation.

The present study systematically investigated the combustion characteristics of spent mushroom substrate (SMS) using TG-MS (thermogravimetric/mass spectrometry) and TG-FTIR (thermogravimetric/Fourier transform infrared spectrometry) under five heating rates. The physicochemical characteristics and combustion index pointed to SMS as a promising biofuel for power generation. The high correlation coefficient of the fitting plots and similar activation energy calculated by various methods indicated that four suitable iso-conversional methods were used. The activation energy varied from 130.06 to 192.95 kJ/mol with a mean value of 171.49 kJ/mol using Flynn-Wall-Ozawa and decreased with the increased conversion degree. The most common emissions peaked at the range of 200-400 °C corresponding to volatile combustion stage, except for CO 2 , NO 2 and NO. The peak CO 2 emission occurred at 439.11 °C mainly due to the combustion of fixed carbon., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

Commercial mushrooms are produced on lignocellulose such as straw, saw dust, and wood chips. As such, mushroom-forming fungi convert low-quality waste streams into high-quality food. Spent mushroom substrate (SMS) is usually considered a waste product. This review discusses the applications of SMS to promote the transition to a circular economy. SMS can be used as compost, as a substrate for other mushroom-forming fungi, as animal feed, to promote health of animals, and to produce packaging and construction materials, biofuels, and enzymes. This range of applications can make agricultural production more sustainable and efficient, especially if the CO 2 emission and heat from mushroom cultivation can be used to promote plant growth in greenhouses.

The aim of this study was to investigate the gaseous emissions (CH 4 , N 2 O, and NH 3 ) and compost quality during the pig manure composting by adding spent mushroom substrate (SMS) as a bulking agent. The control treatment was also studied using corn stalk (CS) as a bulking agent. The experiment was conducted in a pilot scale composting reactor under aerobic condition with the initial C/N ratio of 20. Results showed that bulking agents significantly affected gaseous emissions and compost quality. Using SMS as a bulking agent improved composting efficiency by shortening the time for maturity. SMS increased germination index and humic acid of the final compost (by 13.44 and 41.94%, respectively) compared with CS. Furthermore, composting with SMS as a bulking agent could reduce nitrogen loss, NH 3 , and N 2 O emissions (by 13.57, 35.56, and 46.48%, respectively) compared with the control. SMS slightly increased CH 4 emission about 1.1 times of the CS. However, a 33.95% decrease in the global warming potential of CH 4 and N 2 O was obtained by adding SMS treatment. These results indicate that SMS is a favorable bulking agent for reducing gaseous emissions and increasing compost quality.

The effects of spent mushroom substrate (SMS) and sucrose (S) amendment on emissions of nitrogenous gas (mainly NH 3 and N 2 O) and end products quality of sewage sludge (SS) composting were evaluated. Five treatments were composted for 20 days in laboratory-scale using SS with different dosages of SMS and S, without additive amended treatment used as control. The results indicated that SMS amendments especially combination with S promoted dehydrogenase activity, CO 2 production, organic matter degradation and humification in the composting, and maturity indices of composting also showed that the 30%SMS+2%S treatment could be much more appropriate to improve the composting process, such as total Kjeldahl nitrogen, nitrification index, humic acids/fulvic acids ratio and germination index, while the emissions of NH 3 and N 2 O were reduced by 34.1% and 86.2%, respectively. These results shown that the moderate addition of SMS and S could improve the compost maturity and reduce nitrogenous gas emission., (Copyright © 2018 Elsevier Ltd. All rights reserved.)