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Start Over Author "Samuel Golas" Topic adipose tissue
4 results on '"Samuel Golas"'

1. The Vasoactive Effect of Perivascular Adipose Tissue and Hydrogen Sulfide in Thoracic Aortas of Normotensive and Spontaneously Hypertensive Rats

Author

Samuel Golas, Andrea Berenyiova, Miroslava Majzunova, Magdalena Drobna, Muobarak J. Tuorkey, and Sona Cacanyiova

Subjects
adipose tissue, H2S, thoracic aorta, Wistar, SHR, essential hypertension, Microbiology, QR1-502
Abstract

The objective of this study was to investigate the vasoregulatory role of perivascular adipose tissue (PVAT) and its mutual interaction with endogenous and exogenous H2S in the thoracic aorta (TA) of adult normotensive Wistar rats and spontaneously hypertensive rats (SHRs). In SHRs, hypertension was associated with cardiac hypertrophy and increased contractility. Regardless of the strain, PVAT revealed an anticontractile effect; however, PVAT worsened endothelial-dependent vasorelaxation. Since H2S produced by both the vascular wall and PVAT had a pro-contractile effect in SHRs, H2S decreased the sensitivity of adrenergic receptors to noradrenaline in Wistar rats. While H2S had no contribution to endothelium-dependent relaxation in Wistar rats, in SHRs, H2S produced by the vascular wall had a pro-relaxant effect. We observed a larger vasorelaxation induced by exogenous H2S donor in SHRs than in Wistar rats. Additionally, in the presence of PVAT, this effect was potentiated. We demonstrated that PVAT of the TA aggravated endothelial function in SHRs. However, H2S produced by the TA vascular wall had a pro-relaxation effect, and PVAT revealed anti-contractile activity mediated by the release of an unknown factor and potentiated the vasorelaxation induced by exogenous H2S. All these actions could represent a form of compensatory mechanism to balance impaired vascular tone regulation.

Published
2022
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3. The Vasoactive Role of Perivascular Adipose Tissue and the Sulfide Signaling Pathway in a Nonobese Model of Metabolic Syndrome

Author

Irena Markova, Anna Zemancikova, Andrea Berenyiova, Miroslava Majzunova, Sona Cacanyiova, Martina Hüttl, Martina Cebova, Hana Malinska, and Samuel Golas

Subjects
Male, 0301 basic medicine, lcsh:QR1-502, Wistar, Adipose tissue, Aorta, Thoracic, Endogeny, 030204 cardiovascular system & hematology, Biochemistry, lcsh:Microbiology, H2S, Norepinephrine, chemistry.chemical_compound, 0302 clinical medicine, Superoxides, Medicine, Endothelial dysfunction, Hypertriglyceridemia, Vasodilation, Adipose Tissue, HTG, Oxidation-Reduction, Signal Transduction, medicine.medical_specialty, Nitric Oxide Synthase Type III, Oxidative phosphorylation, Article, metabolic syndrome, Nitric oxide, Contractility, 03 medical and health sciences, Internal medicine, perivascular adipose tissue, Animals, Rats, Wistar, Molecular Biology, Superoxide Dismutase, business.industry, Cystathionine gamma-Lyase, isolated artery, medicine.disease, equipment and supplies, Disease Models, Animal, 030104 developmental biology, Endocrinology, chemistry, Vasoconstriction, Endothelium, Vascular, business, Dyslipidemia
Abstract

The aim of this study was to evaluate the mutual relationship among perivascular adipose tissue (PVAT) and endogenous and exogenous H2S in vasoactive responses of isolated arteries from adult normotensive (Wistar) rats and hypertriglyceridemic (HTG) rats, which are a nonobese model of metabolic syndrome. In HTG rats, mild hypertension was associated with glucose intolerance, dyslipidemia, increased amount of retroperitoneal fat, increased arterial contractility, and endothelial dysfunction associated with arterial wall injury, which was accompanied by decreased nitric oxide (NO)-synthase activity, increased expression of H2S producing enzyme, and an altered oxidative state. In HTG, endogenous H2S participated in the inhibition of endothelium-dependent vasorelaxation regardless of PVAT presence, on the other hand, aortas with preserved PVAT revealed a stronger anticontractile effect mediated at least partially by H2S. Although we observed a higher vasorelaxation induced by exogenous H2S donor in HTG rats than in Wistar rats, intact PVAT subtilized this effect. We demonstrate that, in HTG rats, endogenous H2S could manifest a dual effect depending on the type of triggered signaling pathway. H2S within the arterial wall contributes to endothelial dysfunction. On the other hand, PVAT of HTG is endowed with compensatory vasoactive mechanisms, which include stronger anti-contractile action of H2S. Nevertheless, the possible negative impact of PVAT during hypertriglyceridemia on the activity of exogenous H2S donors needs to be taken into consideration.

Published
2021
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4. Arterial Hypertension and Plasma Glucose Modulate the Vasoactive Effects of Nitroso-Sulfide Coupled Signaling in Human Intrarenal Arteries

Author

Samuel Golas, Anton Misak, Zuzana Valaskova, Katarina Krskova, Sona Cacanyiova, Stefan Zorad, Andrea Berenyiova, K. Frimmel, Jan Breza, and M Drobna

Subjects
Blood Glucose, Male, hydrogen sulfide, Pharmaceutical Science, Adipose tissue, Endogeny, 01 natural sciences, Analytical Chemistry, S-Nitrosoglutathione, chemistry.chemical_compound, Renal Artery, Drug Discovery, S-nitrosoglutathione, Endothelial dysfunction, chemistry.chemical_classification, 0303 health sciences, normotensive, Long-term potentiation, Middle Aged, Glutathione, Vasodilation, Protein Transport, human lobar artery, Chemistry (miscellaneous), Hypertension, Molecular Medicine, Female, Signal transduction, Signal Transduction, Serotonin, arterial hypertension, nitroso-sulfide signaling, medicine.medical_specialty, Cystathionine beta-Synthase, Sulfides, Nitric Oxide, 010402 general chemistry, Gene Expression Regulation, Enzymologic, Article, CBS, lcsh:QD241-441, 03 medical and health sciences, Thoracic Arteries, Mediator, lcsh:Organic chemistry, Internal medicine, medicine, Animals, Humans, immunofluorescence, Physical and Theoretical Chemistry, 030304 developmental biology, CTH gene expression, Organic Chemistry, Cystathionine gamma-Lyase, medicine.disease, Rats, 0104 chemical sciences, Enzyme, Endocrinology, chemistry
Abstract

We have investigated the vasoactive effects of the coupled nitro-sulfide signaling pathway in lobar arteries (LAs) isolated from the nephrectomized kidneys of cancer patients: normotensive patients (NT) and patients with arterial hypertension (AH). LAs of patients with AH revealed endothelial dysfunction, which was associated with an increased response to the exogenous NO donor, nitrosoglutathione (GSNO). The interaction of GSNO with the H2S donor triggered a specific vasoactive response. Unlike in normotensive patients, in patients with AH, the starting and returning of the vasorelaxation induced by the end-products of the H2S-GSNO interaction (S/GSNO) was significantly faster, however, without the potentiation of the maximum. Moreover, increasing glycemia shortened the time required to reach 50% of the maximum vasorelaxant response induced by S/GSNO products so modulating their final effect. Moreover, we found out that, unlike K+ channel activation, cGMP pathway and HNO as probable mediator could be involved in mechanisms of S/GSNO action. For the first time, we demonstrated the expression of genes coding H2S-producing enzymes in perivascular adipose tissue and we showed the localization of these enzymes in LAs of normotensive patients and in patients with AH. Our study confirmed that the heterogeneity of specific nitroso-sulfide vasoactive signaling exists depending on the occurrence of hypertension associated with increased plasma glucose level. Endogenous H2S and the end-products of the H2S-GSNO interaction could represent prospective pharmacological targets to modulate the vasoactive properties of human intrarenal arteries.

Published
2020

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